Resource data sheet
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pTBR101-CM (#RDB15972)

Bifidobacterium longum–E. coli shuttle vector.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Hirayama, Y., Appl. Environ. Microbiol. 78 (14): 4984-4994 (2012). PMID 22582061. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Bifidobacterium longum–E. coli shuttle vector. Efficiently transform B. longum 105-A.
Comment For selection of E. coli transformants of this plasmid, the depositor recommends use of both Cm (2.5 microgram/mL) and Ap (100 microgram/mL) because Cm resistance conferred by this plasmid is not strong. For selection of B. longum 105-A transformants, the depositor uses Cm at a concentration of 2.5 microgram/mL. They can successfully select true transformants at this concentration, but it depends on the Bifidobacterium strain. If endogenous Cm resistance is higher than 2.5 microgram/mL in your strain, the depositor recommends change of the CmR marker to the other appropriate markers that you used might be necessary. CmR region can be easily removed from pTBR101-CM by HindIII digestion. Please refer Hirayama et al., AEM, 78, 4984-4994, 2012 for further information. B. longum 105-A (JCM 31944) is available from JCM as a host strain.
Vector backbone pTBR101-CM (Plasmid)
Size of vector backbone 8.4 kb
Selectable markers Cm^r, Amp^r
Reference of insert sequence AB688000 (DDBJ accession number)
Depositor|Developer Fukiya, Satoru | Kano, Yasunobu |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. (Hirayama et al., Appl. Environ. Microbiol., 78(14):4984-4994 (2012)). The availability of the BIOLOGICAL RESOURCE is limited to a not-for-profit academic purpose.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hirayama et al., Appl. Environ. Microbiol., 78(14):4984-4994 (2012))。本リソースは，非営利の学術研究に限って提供する。

Catalog # Resource name Shipping form Fee
RDB15972 pTBR101-CM DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pTBR101-CM was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15972).

Reference section:

Hirayama, Y., Sakanaka, M., Fukuma, H., Murayama, H., Kano, Y., Fukiya, S., Yokota, A., Development of a double-crossover markerless gene deletion system in Bifidobacterium longum: functional analysis of the α-galactosidase gene for raffinose assimilation. Appl. Environ. Microbiol. 78 (14): 4984-4994 (2012). PMID 22582061. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Hirayama, Y., Development of a double-crossover markerless gene deletion system in Bifidobacterium longum: functional analysis of the α-galactosidase gene for raffinose assimilation. Appl. Environ. Microbiol. 78 (14): 4984-4994 (2012). PMID 22582061. [PubMed] [Article] [RRC of NBRP]
reference Nishiyama, K., Bifidobacterium bifidum Extracellular Sialidase Enhances Adhesion to the Mucosal Surface and Supports Carbohydrate Assimilation. MBio 8 (5). pii: e00928-17 (2017). PMID 28974612. [PubMed] [Article]
reference Fukiya, S., Development of practical gene-mutagenesis systems in bifidobacteria. (Japanese text) Seibutsu-kogaku 94 (3): 110-116 (2016).