Please review the QC test results indicated by icon below as well as clone information before placing your order.

pTBR101-CM

Bifidobacterium longum–E. coli shuttle vector.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. (Hirayama et al., Appl. Environ. Microbiol., 78(14):4984-4994 (2012)). The availability of the BIOLOGICAL RESOURCE is limited to a not-for-profit academic purpose.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hirayama et al., Appl. Environ. Microbiol., 78(14):4984-4994 (2012))。本リソースは，非営利の学術研究に限って提供する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15972_A8Fbp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pBS423_seq3 Region: rep A 3',rep B Sequence file: RDB15972_A8Fba.seq
>D04829A1-A8Fb_5_pBS423_seq3_A01_01_ABI08.ab1     1 TGGGGCCGCC GGGGTGAAAA GGCTTCGTTT GTTGACGTTG ATTCGTCTGA GGGCCGTTTA    61 ATGAGCCTGT GGGGGCATCG GGACATGCCG CCCAACTTCA TCGCGGAGAA TCCGGCCAAC   121 GGCCACTGCC ACGCGGGCTG GGTGCTCACC GAACCCGTCT GCCGCACCGA CATGGCCAGA   181 CTCAAGCCCC TGAAGCTCCT GCACGCCGTC ACCGAGGGGC TGCGCCGCAG CGTGGACGGC   241 GACGAGGGCT ATTCCGGCCT GCTCATGAAG AACCCGTTGA GCGACGCGTG GGACAGCGAC   301 CTGTGCCGCG AGGACACCTA CGACCTGCCC GACCTCGTGG CCGCGCTCGA AGCGCACGGG   361 GACATGCCGC CCAAGAGCTG GACGCGCACC AAACGCGCCC GCGAGGTCGG AGTGGGCCGC   421 AACTGCACCC TGTTCGACGA GGCCCGCACC CTCGCCTACC GCGAGGTGCG CCGACTGCCT   481 GACCGCACGC CCGCCTCCTC CGACCTGCTG CGCGAGTACG TGCGCCGCAC CTGCCACGAA   541 ATCAACGCCT CGTTCCCCGA CCCGCTGCCC GTGCGCGAGG TCAACGACAC CGCCAAGAGC   601 ATCCACAAGT GGATCACCAC GCGAAGCCGC ATGTGGCGAG ACGGTGCCGT CGCCAACGCC   661 GCCACGTTCG TCGCCATCCA ATCCGCACGC GGAAAGAAAT CGGGCGAGGC CAGACAAAAC   721 GCATTTGAAG AGAAGTTCGC GCAGTACGCG CAGGAGGTGC TTGGACAATG ACCATCCAGA   781 CAATTCGCAA GAAGCGCCCA TTGCCCGCCA AGGAGTTAGC CGAGGCCTAT GGGGTGTCCG   841 TGCGCACGAT CAAGTACTGG AATTCGCAAA CGCGCGAGGG ACTGGATAGA CGAGCAGGCA   901 ACGTTGCGCG AATCCATCCG CGCCTACCAC GACGATGACG GGCCACTCGT GGAGCCAGAC   961 CGCCGAACAC TTCAACATGA CCCAAGGTGC CGTGCGCCAA CGCGCCTACC GCGGCCCGCA  1021 AGGGAAACGC CGGAGGTCGA GGCCAAAAGC TCGCAAGGGC CCGGGAAGTA GCAAAGTGGA  1081 CGTGAACGTT ATATATTACG TTCAAACTTC GGATTTCGGC GGGGTGCCGG AAGTCGGCTT 1141 GGCCCGGGGG AAAAAGAATG C //
Primer: pTBR101CM_seq7 Region: rep B,pTB4_P2,pTB4_p3 Sequence file: RDB15972_A8Fbb.seq
>D04829A1-5_A8Fb_pTBR101CM_seq7_B01_02.ab1     1 TGAAAGGGGC GGCCGAATCC ATCCGCGCCT ACCACGACGA TGACGGCCAC TCGTGGAGCC    61 AGACCGCCGA ACACTTCACC ATGACCCAAG GTGCCGTGCG CCAACGCGCC TACCGCGCCC   121 GCAAGGAACG CGAGGCCGAG GCAAAAGCCG CAAGGCCGGA GTAGCAAGTG ACGTGAACGT   181 ATATACGTTC ACTCGATTCG CGGTGCGAAT CGGCTTGCCG GGAAGACCCA TGCCCCATAG   241 TCGGCAGAGC CTCCAGACCG CTCACGCGGC ATCGTGCCAC GACTGTGGCA CATCACGTAA   301 CACCCGCACA TCCTCACGGT GGTGCGCGGG TGTTACGTGA TTCGAACCAT CGGCCTGACA   361 GGTCTCAGAT CGCTCCAGCG AGCTTACGGC GCATGTACTC GCTGCGGCTG ATGTGGTACC   421 GCCTGGCCAT CGCGGTCAGA CCGTCCAGCG TGCTCTTGGG CATGCGGACG GACACGCTGA   481 CCATCTGCTC GTCGGGCTCG GCGAGATGGA GCCCGTAGTA CACGCGCCCC GTCAGGTCGT   541 CCGGGACAGT CTCGGACTCG GCCATCCGCT CATCCTCCAG CACCTGCTCC TCGGTCAGCC   601 CGACCAGCGC CATCAACTGC CTGTCGCGTT CGCTGAAATC ACTCATAGCG TCCTCCCATC   661 GCCGCGCAGA CGGTCTATCT CGCGCCTGAA CTTCTTGGTC GGCGGCGTGA ACGCGTGGTA   721 GATCACCACG AGGTCGCCGA CGACCCTGTA CAGCATCTCG ACGTTCCTGC CGCGCCCGTC   781 GAGCCCTATC GCCATCCACG CGCCGGACTC GCGCTCCGCG TCCACCATGA CGCTGCGGAA   841 CGCGGTCACC ACGTCCTTCT CGGACAGTTC TGGGTGACGC TCCATCACCC TGTCAAAGCA   901 CCTGGGATCC TCTACGCCGG ACGCATCGTG GCCGGCATCA CCGGCGCCAC AGGTGCGGTT   961 GCTGGCGCCT ATATCGCCGA ACATCACCGA TGGGGGAAAG AATCGGCTTC GCCACTTCGG  1021 CTTCAATGGA GCGGCTTTGT TTCGGCGTGG GTATTGGGTG GTCAGCCTCG TGGCCCGGGG 1081 AACTTGATGG GCGCCAATCA TCGTTGCAAT GCAACCCAAT TCCTTTTGTC G //
Primer: pTBR101CM_seq3 Region: pTB4_p1,repA 5' Sequence file: RDB15972_A8Fbc.seq
>D04829A1-A8Fb_5_pTBR101CM_seq3_B01_02_ABI08.ab1     1 TTGCTTGTGT TAATCCCCCG CGGAGGCGGT CTGGAGGCTC CGCCGGACCA TGGGGCATGG    61 GTCTTCCCGG CAAGCCGATT CGCACCGCGA ATCGAGTGAT TGTAGCTACA ATCACGTCGC   121 TTGCTACTCC GGCCTTCGGC ACTCGGGTTG TTGCTCCTGC GCCGAAGGGC GAGGGAGGCT   181 GCGGGCTCGG TTCATGTACA TGAACCGTCT AGCTTGCTTA CCTTCGATTT GATGGACATC   241 TCCATGTGGA TGTCCATGGA GATGTCCATG GAGATGTCCA TGGAGATGTC CATGGAGATG   301 TCCATGGAGA TGTCCATGTG GGTGTCCATG TGGGTGTCCA TGGATATATC CATCTGAGCG   361 CATAGGAAAA CGCATGAGGG TGGATGATCG TTCGGGGTGA TGCCTTCTTC CACGAACGGG   421 AAGGGGCTCT TCCTTTTTCT GGAATGTCAT CTTCCAGCGC CCTTCGCCTT GAGCTCCTTG   481 AGCAGCATCG TCGCGTTGTC GCGGATGCTC GCGTCCGTGA GCGGGGAGAG CTCGTCCGCA   541 TAGGAGCGGC AGAGGAGCGC GTACACCCGT TCCCGCACCC AGGCGCTGCG CGCGATGCCC   601 AGCTTGAACG CGGCCCGGCA CAGCATCTCG TATTCGTCGC CGGTGAGCAG GACATTGAGC   661 TGGCGTCAAA AGTTGATTAG TCATGCCGTG TATATACGTC CGGGGTGTTT TCGGCCAGAT   721 TCCAACAGTT TTGGGCCACT CCGGGAAGGA ACAGCCGACG CTTCGCTGGG CTGAACCATC   781 TGACTTGGTT CGGCGCACGC TTGTGCAATG CACAAATGTG CAATAGGATG TGAGCTATGA   841 GCAACGAAAT ATGTGCAGGG GGACGCTGGA GCTGACGCGG GCGTTCGACG GGCTGGGTGG   901 CTTCCCCAGC GTCCCCTGTG GCTGCGACGA TGACTACTCC CAGCTGGGTG CGGCCGGAAG   961 CCGCATCGAA CGCGGCTCAG AATGGCAAGG CACATCGAAG GCGAAATCCG GCCAGCCGCA  1021 AGGTGGAACA TTGCTCCGTT GTTTGGAACG TTGGATTTCG CTCTTGAGGG CCCGTTTAAA  1081 TGGAAGCCCT TGTGGGGGGC CATCCGGGGA ACATGTGCCC GGCCCCAAAC CTTTTCCATC  1141 CGCGGAGAAA TCCGGGCCCA ACGGGGCCAC CTGTGTCCCC ACGCCCGCCG GGGCCGATGT 1201 GGGAGTTGG //
Primer: Amp_R Region: Amp R pro,Cm R Sequence file: RDB15972_A8Fbd.seq
>D04829A1-A8Fb_5_Amp_R_D01_04_ABI08.ab1     1 CCCCTTTTTT TAATAATTAT TGAGCATTTA TCAGGGTTAA TTGTCTCATG AGCGGATACA    61 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG   121 TGCCACCTGA CGTCTAAGAA ACCATTATTA TCATGACATT AACCTATAAA AATAGGCGTA   181 TCACGAGGCC CTTTCGTCTT CAAGAATTCT CATGTTTGAC AGCTTATCAT CGATAAGCTT   241 GGATCTGGAG CTGTAATATA AAAACCTTCT TCAACTAACG GGGCAGGTTA GTGACATTAG   301 AAAACCGACT GTAAAAAGTA CAGTCGGCAT TATCTCATAT TATAAAAGCC AGTCATTAGG   361 CCTATCTGAC AATTCCTGAA TAGAGTTCAT AAACAATCCT GCATGATAAC CATCACAAAC   421 AGAATGATGT ACCTGTAAAG ATAGCGGTAA ATATATTGAA TTACCTTTAT TAATGAATTT   481 TCCTGCTGTA ATAATGGGTA GAAGGTAATT ACTATTATTA TTGATATTTA AGTTAAACCC   541 AGTAAATGAA GTCCATGGAA TAATAGAAAG AGAAAAAGCA TTTTCAGGTA TAGGTGTTTT   601 GGGAAACAAT TTCCCCGAAC CATTATATTT CTCTACATCA GAAAGGTATA AATCATAAAA   661 CTCTTTGAAG TCATTCTTTA CAGGAGTCCA AATACCAGAG AATGTTTTAG ATACACCATC   721 AAAAATTGTA TAAAGTGGCT CTAACTTATC CCAATAACCT AACTCTCCGT CGCTATTGTA   781 ACCAGTTCTA AAAGCTGTAT TTGAGTTTAT CACCCTTGTC ACTAAGAAAA TAAATGCAGG   841 GTAAAATTTA TATCCTTCTT GTTTTATGTT TCGGGTATAA AACACTAATA TCAATTTTCT   901 GTGGTTATAC TAAAAGTCGT TTGTTGGGTT CAAATAATGA TTAAATATCT CTTTTCTCTT   961 CCAATTTGTC TAAATCAATT TTATTAAAGG TTCATTTGAT ATGCCTTCCT AAATTTTTAT  1021 CTAAAGTGAA ATTAGGAGGC TTACTTTGTC TGCTTTCTTC ATTAGAATCA ATCCTTTTTT  1081 AAAAGTCATA TTAACTGGTA ACGTAAATAT GTATTTTGAA AAATAATCCC AACTTTTTAT 1141 CCCAATTTTT TCGGTAATG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl