Please review the QC test results indicated by icon below as well as clone information before placing your order.

pTBR101-CM

Bifidobacterium longum–E. coli shuttle vector.

Sequence (full)	RDB15972zzk01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. (Hirayama et al., Appl. Environ. Microbiol., 78(14):4984-4994 (2012)). The availability of the BIOLOGICAL RESOURCE is limited to a not-for-profit academic purpose.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hirayama et al., Appl. Environ. Microbiol., 78(14):4984-4994 (2012))。本リソースは，非営利の学術研究に限って提供する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19984_B3Eip1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: tetA_mid_F (Pr1320) Region: insert(pTB4_p1 3') Sequence file: RDB19984_B3Eia.seq
>15972_19984_B3Ei_2_tetA_mid_F_A01_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN GGNNNCNNTA TCGNCTACGC GATCATGGCG NCCACACCCG    61 TCCTGTGGAT CCTCATCAGC ACCACCCGCG ACACGAACGT ATTACATACG CAATCATATC   121 AGTGTCACAC ATCAGGTACA ACCGTACCGC AACACGCTTC GAGCGGACGC AAGCGGCTTT   181 TGCGCGGAGC ATTTCCGGCC ATTTGCCCAG CTTGTTCCGC GCTTCCGAGA AGGTGTGGAG   241 TTGGGTTTTC TGTGATGCCG CGCGAGCGGT CTGGAGGCTC CGCCGACCAT GGGGCATGGG   301 TCTTCCCGGC AAGCCGATTC GCACCGCGAA TCGAGTGATT GTAGCTACAA TCACGTCGCT   361 TGCTACTCCG GCCTTCGGCA CTCGGGTTGT TGCTCCTGCG CCGAAGGGCG AGGGAGGCTG   421 CGGGCTCGGT TCATGTACAT GAACCGTCTA GCTTGCTTAC CTTCGATTTG ATGGACATCT   481 CCATGTGGAT GTCCATGGAG ATGTCCATGG AGATGTCCAT GGAGATGTCC ATGGAGATGT   541 CCATGGAGAT GTCCATGTGG GTGTCCATGT GGGTGTCCAT GGATATATCC ATCTGAGCGC   601 ATAGGAAAAC GCATGAGGGT GGATGATCGT TCGGGGTGAT GCCTTCTTCC NCGAACGGGA   661 AGGGGCTCTT CCTTTTTCTG GAATGTCATC TTCCAGCGCC CTTCGCCTTG AGCTCCTTGA   721 NCAGCATCGT CGCGTTGTCG CGGATGCTCG CGTCCGTGAN CGGGGANAGC TCGTCCGCAT   781 ANGANCGGCA GANGANCGCG TACANNNNNT CCCGCACCCA GGCGCTGCGC GCGATGCCCA   841 NCTTGAACGC GGCCCGGCNC ANCATCTCGT ATNCGTCGCC NNNGANCAGG NNNTTGANCT   901 NGNGNNNAAA NNNGATTANN CNTGNCNNNN ATATACNNNC CGGGGNNNTT NNNNNNNNNN //
Primer: lacIpro_R1 (Pr0762) Region: insert(pTB4_p3,pTB4_p2) Sequence file: RDB19984_B3Eib.seq
>15972_19984_B3Ei_2_lacIpro_R1_E01_05_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNACNN GCGCTCNTGA NCCCGAAGTG GCGAGCCCGA    61 TCTTCCCCAT CGGTGANGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG   121 ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCCAGGTGC TTGACAGGGT GATGGAGCGT   181 CACCCAGAAC TGTCCGAGAA GGACGTGGTG ACCGCGTTCC GCAGCGTCAT GGTGGACGCG   241 GAGCGCGAGT CCGGCGCGTG GATGGCGATA GGGCTCGACG GGCGCGGCAG GAACGTCGAG   301 ATGCTGTACA GGGTCGTCGG CGACCTCGTG GTGATCTACC ACGCGTTCAC GCCGCCGACC   361 AAGAAGTTCA GGCGCGAGAT AGACCGTCTG CGCGGCGATG GGAGGACGCT ATGAGTGATT   421 TCAGCGAACG CGACAGGCAG TTGATGGCGC TGGTCGGGCT GACCGAGGAG CAGGTGCTGG   481 AGGATGAGCG GATGGCCGAG TCCGAGACTG TCCCGGACGA CCTGACGGGG CGCGTGTACT   541 ACGGGCTCCA TCTCGCCGAG CCCGACGAGC AGATGGTCAG CGTGTCCGTC CGCATGCCCA   601 AGAGCACGCT GGACGGTCTG ACCGCGATGG CCAGGCGGTA CCACATCAGC CGCAGCGAGT   661 ACATGCGCCG TAAGCTCGCT GGAGCGATCT GAGACCTGTC AGGCCGATGG TTCGAATCAC   721 GTAACACCCG CGCACCACCG TGAGGATGTG CGGGTGTTAC GTGATGTGCC ACAGTCGTGG   781 CACGATGCCG CGNGAGCGGT CTGGANGCTC TGCCGACTAN NGGGGCATGG GTCTTCCCNG   841 CNNGCCGATT CNCACCNCNN NNCGAGNGNA NCGTATATNC GTNCACGTNN NTTGNNACNN 901 CNNGNNNNNN NNNN //
Primer: pBS423_seq3_3 (Pr0751) Region: insert(repA mid) Sequence file: RDB19984_B3Eic.seq
>15972_19984_B3Ei_2_pBS423_seq3_3_G01_07_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNGNGNCTT CCCCNNCGTC CCCTGTGCTG CGNCGATGAC    61 TACTCCCAGC TGGTGCNNNN GNNCCGCATC GACGCGCTCA GATGCAAGCA CATCGAGGCG   121 AATCCGGCAG CGCAGGTGAA CATGCTCGTT GTTGACGTTG ATTCGTCTGA GGGCCGTTTA   181 ATGAGCCTGT GGGGGCATCG GGACATGCCG CCCAACTTCA TCGCGGAGAA TCCGGCCAAC   241 GGCCACTGCC ACGCGGGCTG GGTGCTCACC GAACCCGTCT GCCGCACCGA CATGGCCAGA   301 CTCAAGCCCC TGAAGCTCCT GCACGCCGTC ACCGAGGGGC TGCGCCGCAG CGTGGACGGC   361 GACGAGGGCT ATTCCGGCCT GCTCATGAAG AACCCGTTGA GCGACGCGTG GGACAGCGAC   421 CTGTGCCGCG AGGACACCTA CGACCTGCCC GACCTCGTGG CCGCGCTCGA AGCGCACGGG   481 GACATGCCGC CCAAGAGCTG GACGCGCACC AAACGCGCCC GCGAGGTCGG AGTGGGCCGC   541 AACTGCACCC TGTTCGACGA GGCCCGCACC CTCGCCTACC GCGAGGTGCG CCGACTGCCT   601 GACCGCACGC CCGCCTCCTC CGACCTGCTG CGCGAGTACG TGCGCCGCAC CTGCCACGAA   661 ATCAACGCCT CGTTCCCCGA CCCGCTGCCC GTGCGCGAGG TCAACGACAC CGCCNAGAGC   721 ATCCACAAGT GGATCACCAC GCGAAGCCGC ATGTGGCGAG ACGGTGCCGT CGCCNACGCC   781 GCCACGTTCG TCGCCATCCA ATCCGCACGC GGAAAGAAAT CNGGGCNANG NCAGACAAAA   841 CGNATTTGAA GANAAGTTCG CGNANTANGC GCANGNNGNG CNTGNNNNNN GANNNTCCNN   901 ANNNNTCNNN AGNNNNNCCN ATTGCCNNNN NNNGNNNNNN NCNNANNNNN NNNGGNNNNN 961 NNNNN //
Primer: repA_F_rev2 (Pr1322) Region: insert(repA 5',pTB4_p1 5') Sequence file: RDB19984_B3Eid.seq
>15972_19984_B3Ei_2_repA_F_rev2_D01_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGC CCTNNGANGA NCAACGTCAA CAACGAGCAT    61 GTTCACCTGC GCTGNNNNNN NTCGCCTCGA TGTGCTTGCA TCTGAGCGCG TCGATGCGGC   121 TCCGGCGCAC CAGCTGGGAG TAGTCATCGT CGCAGCACAG GGGACGCTGG GGAAGCCACC   181 AGCCGTCGAA CGCCCGCGTC AGCTCCAGCG TCCCCTGCAC ATATTCGTTG CTCATAGCTC   241 ACATCCTATT GCACATTTGT GCATTGCACA AGCGTGCGCC GAACCAAGTC AGATGGTTCA   301 GCCCAGCGAA GCGTCGGCTG TTCCTTCCCG GAGTGGCCCA AAACTGTTGG AATCTGGCCG   361 AAAACACCCC GGACGTATAT ACACGGCATG ACTAATCAAC TTTTGACGCC AGCTCAATGT   421 CCTGCTCACC GGCGACGAAT ACGAGATGCT GTGCCGGGCC GCGTTCAAGC TGGGCATCGC   481 GCGCAGCGCC TGGGTGCGGG AACGGGTGTA CGCGCTCCTC TGCCGCTCCT ATGCGGACGA   541 GCTCTCCCCG CTCACGGACG CGAGCATCCG CGACAACGCG ACGATGCTGC TCAAGGAGCT   601 CAAGGCGAAG GGCGCTGGAA GATGACATTC CAGAAAAAGG AAGAGCCCCT TCCCGTTCGT   661 GGAAGAAGGC ATCACCCCGA ACGATCATCC ACCCTCATGC GTTTTCCTAT GCGCTCAGAT   721 GGATATATCC ATGGACACCC ACATGGACAC CCACATGGAC ATCTCCATGG ACATCTCCAT   781 GGACATCTCC ATGGNCATCT CCATGGACAT CTCCATGNAC ATCCACATGG AGATGTCCAT   841 CAAATCNNAN NNTNNNCAAG CTAGANGGTN CATGTACNTG AANCGANCCC GCNNCCTCCN 901 NNNNCNTNCN GNNNNNNNCN NCNNNCCNAN NGNCGAANNN NNNNNNN //
Primer: repB_F_rev (Pr1321) Region: insert(repB 5',repA 3') Sequence file: RDB19984_B3Eie.seq
>15972_19984_B3Ei_2_repB_F_rev_E01_05_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNANG CCTCGGCTAA CTCCTTGGCG GGCAATGGGC    61 GCTTCTTGCG AATTGNNTGG ATGGTCATTG TCCAAGCACC TCCTGCGCGT ACTGCGCGAA   121 CTTCTCTTCA AATGCGTTTT GTCTGGCCTC GCCCGATTTC TTTCCGCGTG CGGATTGGAT   181 GGCGACGAAC GTGGCGGCGT TGGCGACGGC ACCGTCTCGC CACATGCGGC TTCGCGTGGT   241 GATCCACTTG TGGATGCTCT TGGCGGTGTC GTTGACCTCG CGCACGGGCA GCGGGTCGGG   301 GAACGAGGCG TTGATTTCGT GGCAGGTGCG GCGCACGTAC TCGCGCAGCA GGTCGGAGGA   361 GGCGGGCGTG CGGTCAGGCA GTCGGCGCAC CTCGCGGTAG GCGAGGGTGC GGGCCTCGTC   421 GAACAGGGTG CAGTTGCGGC CCACTCCGAC CTCGCGGGCG CGTTTGGTGC GCGTCCAGCT   481 CTTGGGCGGC ATGTCCCCGT GCGCTTCGAG CGCGGCCACG AGGTCGGGCA GGTCGTAGGT   541 GTCCTCGCGG CACAGGTCGC TGTCCCACGC GTCGCTCAAC GGGTTCTTCA TGAGCAGGCC   601 GGAATAGCCC TCGTCGCCGT CCACGCTGCG GCGCAGCCCC TCGGTGACGG CGTGCANGAG   661 CTTCAGGGGC TTGAGTCTGG CCATGTCGGT GCGGCAGACG GGTTCGGTGA GCACCCAGCC   721 CGCGTGGCAG TGGCCGTTGG CCGGANTCTC CGCGATGAAG TTGGGCGGCA TGTCCCGATG   781 CCCCCACANG CTCATTAAAC GGCCCTCAGA CNAATCANCG TCNACAACNA NCATGTTCAC   841 CTGCGCNGNC NGNATTCNCC NCNATGNGCT NGNNTCTGAN CGCGNNNATG NNGNNNCNNG 901 NNNANNNNCN GGGNNNANNN ATCNNCNNNN CNNNNGGNNN NNNNGGNNNN NNNNNN //
Primer: pTBR101CM_seq7 (Pr0561) Region: insert(repB 3',pTB4_p2,pTB4_p3 3') Sequence file: RDB19984_B3Eif.seq
>15972_19984_B3Ei_2_pTBR101CM_seq7_F01_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNNTCC NNCGNCTNNN NCGNNNNNGN NGGCNNCTCG TGGNNCCAGA    61 CCGCCGAACA CTTCAACNTG NCCCAAGGTG CCGTGCGCCA ACGCGCCTAC CGCGCCCGCA   121 AGGAACGCGA GGCCGAGGCA AAAGCCGCAA GGCCGGAGTA GCAAGTGACG TGAACGTATA   181 TACGTTCACT CGATTCGCGG TGCGAATCGG CTTGCCGGGA AGACCCATGC CCCATAGTCG   241 GCAGAGCCTC CAGACCGCTC ACGCGGCATC GTGCCACGAC TGTGGCACAT CACGTAACAC   301 CCGCACATCC TCACGGTGGT GCGCGGGTGT TACGTGATTC GAACCATCGG CCTGACAGGT   361 CTCAGATCGC TCCAGCGAGC TTACGGCGCA TGTACTCGCT GCGGCTGATG TGGTACCGCC   421 TGGCCATCGC GGTCAGACCG TCCAGCGTGC TCTTGGGCAT GCGGACGGAC ACGCTGACCA   481 TCTGCTCGTC GGGCTCGGCG AGATGGAGCC CGTAGTACAC GCGCCCCGTC AGGTCGTCCG   541 GGACAGTCTC GGACTCGGCC ATCCGCTCAT CCTCCAGCAC CTGCTCCTCG GTCAGCCCGA   601 CCAGCGCCAT CAACTGCCTG TCGCGTTCGC TGAAATCACT CATAGCGTCC TCCCATCGCC   661 GCGCAGACGG TCTATCTCGC GCCTGAACTT CTTGGTCGGC GGCGTGAACG CGTGGTAGAT   721 CACCACGANG TCGCCGACGA NCCTGTACAG CATCTCGACG TTCCTGCCGC GCCCGTCGAN   781 NCCTATCGCC ATCCACGCGC CGGANTCGCG CTCCGCGTCC ACCATGACGC TGCGGAACGC   841 GGNCACCACG TCCTTCTCGG ANAGTTCTGG GNGANGCTNC ATCACCCNGN NNNGCNCCNN   901 NNNTNCNCTA CNCCNNANGC NTCNNNNNCN NGNNTCACNN GCNNNNNNNG NNNNNGNNNN 961 NNGNNNNNNN NNN //
Primer: pBR_EcoRI_R (Pr0139) Region: CmR Sequence file: RDB19984_B3Eig.seq
>15972_19984_B3Ei_2_pBR_EcoRI_R_C01_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNCGCNNTGT NAGNNTTNNT ACACGGNGCC TGNNTGCGTT    61 AGCAATTTAA CTGTGATANN NNACCGCATT AACAAGCTTG CGGCAATAGT TACCCTTATT   121 ATCAAGATAA GAAAGAAAAG GATTTTTCGC TACGCTCAAA TCCTTTAAAA AAACACAAAA   181 GACCACATTT TTTAATGTGG TCTTTTATTC TTCAACTAAA GCACCCATTA GTTCAACAAA   241 CGAAAATTGG ATAAAGTGGG ATATTTTNAA AATATATATT TATGTTACAG TAATATTGAC   301 TTTTAAAAAA GGATTGATTC TAATGAAGAA AGCAGACAAG TAAGCCTCCT AAATTCACTT   361 TAGATAAAAA TTTAGGAGGC ATATCAAATG AACTTTAATA AAATTGATTT AGACAATTGG   421 AAGAGAAAAG AGATATTTAA TCATTATTTG AACCAACAAA CGACTTTTAG TATAACCACA   481 GAAATTGATA TTAGTGTTTT ATACCGAAAC ATAAAACAAG AAGGATATAA ATTTTACCCT   541 GCATTTATTT TCTTAGTGAC AAGGGTGATA AACTCAAATA CAGCTTTTAG AACTGGTTAC   601 AATAGCGACG GAGAGTTAGG TTATTGGGAT AAGTTAGAGC CACTTTATAC AATTTTTGAT   661 GGTGTATCTA AAACATTCTC TGGTATTTGG ACTCCTGTAA AGAATGACTT CAAAGAGTTT   721 TATGATTTAT ACCTTTCTGA NGTAGAGAAN TATAATGGTT CGGGGAAATT GTTTCCCNAA   781 ACACCTATAC CTGAAAATGC TTTTTCTCTT TCTATTATTC CATGGACTTC ATTTACTGGG   841 TTTAACTTAA ANTATCAATA ATAATANNAN NTNNCTTCTA CCCNTTATTA CAGCNNNAAA   901 ANTCATTANN NAANGNAATT CNANNTATTT ANNNCTNTNT TTACNNGNNN NNTNNNNNNN 961 NTNNNNNNNN NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.06.14

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