Sequencing and PCR primers for primer information.
Bifidobacterium longum–E. coli shuttle vector.
| Catalog number | RDB15972 |
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| Resource name | pTBR101-CM |
| Clone info. | Bifidobacterium longum–E. coli shuttle vector. Efficiently transform B. longum 105-A. For selection of E. coli transformants of this plasmid, the depositor recommends use of both Cm (2.5 microgram/mL) and Ap (100 microgram/mL) because Cm resistance conferred by this plasmid is not strong. For selection of B. longum 105-A transformants, the depositor uses Cm at a concentration of 2.5 microgram/mL. They can successfully select true transformants at this concentration, but it depends on the Bifidobacterium strain. If endogenous Cm resistance is higher than 2.5 microgram/mL in your strain, the depositor recommends change of the CmR marker to the other appropriate markers that you used might be necessary. CmR region can be easily removed from pTBR101-CM by HindIII digestion. Please refer Hirayama et al., AEM, 78, 4984-4994, 2012 for further information. B. longum 105-A (JCM 31944) is available from JCM as a host strain. |
| Vector backbone | pTBR101-CM (Plasmid) |
| Size of vector backbone | 8.4 kb |
| Selectable markers | Cm^r, Amp^r |
| Reference of nucleotide sequence | AB688000 (DDBJ accession number) |
| Depositor | Fukiya, Satoru | Kano, Yasunobu | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. (Hirayama et al., Appl. Environ. Microbiol., 78(14):4984-4994 (2012)). The availability of the BIOLOGICAL RESOURCE is limited to a not-for-profit academic purpose. |
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| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB15972 | pTBR101-CM | DNA solution |
Electronic file
Original reference
Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Bacteria host strains (en) (English text) |
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| Featured content | Bacteria host strains (ja) (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB15972_A8Fbp1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: pBS423_seq3 | Sequence: RDB15972_A8Fba.seq |
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>D04829A1-A8Fb_5_pBS423_seq3_A01_01_ABI08.ab1 1 TGGGGCCGCC GGGGTGAAAA GGCTTCGTTT GTTGACGTTG ATTCGTCTGA GGGCCGTTTA 61 ATGAGCCTGT GGGGGCATCG GGACATGCCG CCCAACTTCA TCGCGGAGAA TCCGGCCAAC 121 GGCCACTGCC ACGCGGGCTG GGTGCTCACC GAACCCGTCT GCCGCACCGA CATGGCCAGA 181 CTCAAGCCCC TGAAGCTCCT GCACGCCGTC ACCGAGGGGC TGCGCCGCAG CGTGGACGGC 241 GACGAGGGCT ATTCCGGCCT GCTCATGAAG AACCCGTTGA GCGACGCGTG GGACAGCGAC 301 CTGTGCCGCG AGGACACCTA CGACCTGCCC GACCTCGTGG CCGCGCTCGA AGCGCACGGG 361 GACATGCCGC CCAAGAGCTG GACGCGCACC AAACGCGCCC GCGAGGTCGG AGTGGGCCGC 421 AACTGCACCC TGTTCGACGA GGCCCGCACC CTCGCCTACC GCGAGGTGCG CCGACTGCCT 481 GACCGCACGC CCGCCTCCTC CGACCTGCTG CGCGAGTACG TGCGCCGCAC CTGCCACGAA 541 ATCAACGCCT CGTTCCCCGA CCCGCTGCCC GTGCGCGAGG TCAACGACAC CGCCAAGAGC 601 ATCCACAAGT GGATCACCAC GCGAAGCCGC ATGTGGCGAG ACGGTGCCGT CGCCAACGCC 661 GCCACGTTCG TCGCCATCCA ATCCGCACGC GGAAAGAAAT CGGGCGAGGC CAGACAAAAC 721 GCATTTGAAG AGAAGTTCGC GCAGTACGCG CAGGAGGTGC TTGGACAATG ACCATCCAGA 781 CAATTCGCAA GAAGCGCCCA TTGCCCGCCA AGGAGTTAGC CGAGGCCTAT GGGGTGTCCG 841 TGCGCACGAT CAAGTACTGG AATTCGCAAA CGCGCGAGGG ACTGGATAGA CGAGCAGGCA 901 ACGTTGCGCG AATCCATCCG CGCCTACCAC GACGATGACG GGCCACTCGT GGAGCCAGAC 961 CGCCGAACAC TTCAACATGA CCCAAGGTGC CGTGCGCCAA CGCGCCTACC GCGGCCCGCA 1021 AGGGAAACGC CGGAGGTCGA GGCCAAAAGC TCGCAAGGGC CCGGGAAGTA GCAAAGTGGA 1081 CGTGAACGTT ATATATTACG TTCAAACTTC GGATTTCGGC GGGGTGCCGG AAGTCGGCTT 1141 GGCCCGGGGG AAAAAGAATG C // |
| Primer: pTB101CM_seq7 | Sequence: RDB15972_A8Fbb.seq |
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>D04829A1-5_A8Fb_pTBR101CM_seq7_B01_02.ab1 1 TGAAAGGGGC GGCCGAATCC ATCCGCGCCT ACCACGACGA TGACGGCCAC TCGTGGAGCC 61 AGACCGCCGA ACACTTCACC ATGACCCAAG GTGCCGTGCG CCAACGCGCC TACCGCGCCC 121 GCAAGGAACG CGAGGCCGAG GCAAAAGCCG CAAGGCCGGA GTAGCAAGTG ACGTGAACGT 181 ATATACGTTC ACTCGATTCG CGGTGCGAAT CGGCTTGCCG GGAAGACCCA TGCCCCATAG 241 TCGGCAGAGC CTCCAGACCG CTCACGCGGC ATCGTGCCAC GACTGTGGCA CATCACGTAA 301 CACCCGCACA TCCTCACGGT GGTGCGCGGG TGTTACGTGA TTCGAACCAT CGGCCTGACA 361 GGTCTCAGAT CGCTCCAGCG AGCTTACGGC GCATGTACTC GCTGCGGCTG ATGTGGTACC 421 GCCTGGCCAT CGCGGTCAGA CCGTCCAGCG TGCTCTTGGG CATGCGGACG GACACGCTGA 481 CCATCTGCTC GTCGGGCTCG GCGAGATGGA GCCCGTAGTA CACGCGCCCC GTCAGGTCGT 541 CCGGGACAGT CTCGGACTCG GCCATCCGCT CATCCTCCAG CACCTGCTCC TCGGTCAGCC 601 CGACCAGCGC CATCAACTGC CTGTCGCGTT CGCTGAAATC ACTCATAGCG TCCTCCCATC 661 GCCGCGCAGA CGGTCTATCT CGCGCCTGAA CTTCTTGGTC GGCGGCGTGA ACGCGTGGTA 721 GATCACCACG AGGTCGCCGA CGACCCTGTA CAGCATCTCG ACGTTCCTGC CGCGCCCGTC 781 GAGCCCTATC GCCATCCACG CGCCGGACTC GCGCTCCGCG TCCACCATGA CGCTGCGGAA 841 CGCGGTCACC ACGTCCTTCT CGGACAGTTC TGGGTGACGC TCCATCACCC TGTCAAAGCA 901 CCTGGGATCC TCTACGCCGG ACGCATCGTG GCCGGCATCA CCGGCGCCAC AGGTGCGGTT 961 GCTGGCGCCT ATATCGCCGA ACATCACCGA TGGGGGAAAG AATCGGCTTC GCCACTTCGG 1021 CTTCAATGGA GCGGCTTTGT TTCGGCGTGG GTATTGGGTG GTCAGCCTCG TGGCCCGGGG 1081 AACTTGATGG GCGCCAATCA TCGTTGCAAT GCAACCCAAT TCCTTTTGTC G // |
| Primer: pTB101CM_seq3 | Sequence: RDB15972_A8Fbc.seq |
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>D04829A1-A8Fb_5_pTBR101CM_seq3_B01_02_ABI08.ab1 1 TTGCTTGTGT TAATCCCCCG CGGAGGCGGT CTGGAGGCTC CGCCGGACCA TGGGGCATGG 61 GTCTTCCCGG CAAGCCGATT CGCACCGCGA ATCGAGTGAT TGTAGCTACA ATCACGTCGC 121 TTGCTACTCC GGCCTTCGGC ACTCGGGTTG TTGCTCCTGC GCCGAAGGGC GAGGGAGGCT 181 GCGGGCTCGG TTCATGTACA TGAACCGTCT AGCTTGCTTA CCTTCGATTT GATGGACATC 241 TCCATGTGGA TGTCCATGGA GATGTCCATG GAGATGTCCA TGGAGATGTC CATGGAGATG 301 TCCATGGAGA TGTCCATGTG GGTGTCCATG TGGGTGTCCA TGGATATATC CATCTGAGCG 361 CATAGGAAAA CGCATGAGGG TGGATGATCG TTCGGGGTGA TGCCTTCTTC CACGAACGGG 421 AAGGGGCTCT TCCTTTTTCT GGAATGTCAT CTTCCAGCGC CCTTCGCCTT GAGCTCCTTG 481 AGCAGCATCG TCGCGTTGTC GCGGATGCTC GCGTCCGTGA GCGGGGAGAG CTCGTCCGCA 541 TAGGAGCGGC AGAGGAGCGC GTACACCCGT TCCCGCACCC AGGCGCTGCG CGCGATGCCC 601 AGCTTGAACG CGGCCCGGCA CAGCATCTCG TATTCGTCGC CGGTGAGCAG GACATTGAGC 661 TGGCGTCAAA AGTTGATTAG TCATGCCGTG TATATACGTC CGGGGTGTTT TCGGCCAGAT 721 TCCAACAGTT TTGGGCCACT CCGGGAAGGA ACAGCCGACG CTTCGCTGGG CTGAACCATC 781 TGACTTGGTT CGGCGCACGC TTGTGCAATG CACAAATGTG CAATAGGATG TGAGCTATGA 841 GCAACGAAAT ATGTGCAGGG GGACGCTGGA GCTGACGCGG GCGTTCGACG GGCTGGGTGG 901 CTTCCCCAGC GTCCCCTGTG GCTGCGACGA TGACTACTCC CAGCTGGGTG CGGCCGGAAG 961 CCGCATCGAA CGCGGCTCAG AATGGCAAGG CACATCGAAG GCGAAATCCG GCCAGCCGCA 1021 AGGTGGAACA TTGCTCCGTT GTTTGGAACG TTGGATTTCG CTCTTGAGGG CCCGTTTAAA 1081 TGGAAGCCCT TGTGGGGGGC CATCCGGGGA ACATGTGCCC GGCCCCAAAC CTTTTCCATC 1141 CGCGGAGAAA TCCGGGCCCA ACGGGGCCAC CTGTGTCCCC ACGCCCGCCG GGGCCGATGT 1201 GGGAGTTGG // |
| Primer: Amp_R | Sequence: RDB15972_A8Fbd.seq |
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>D04829A1-A8Fb_5_Amp_R_D01_04_ABI08.ab1 1 CCCCTTTTTT TAATAATTAT TGAGCATTTA TCAGGGTTAA TTGTCTCATG AGCGGATACA 61 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG 121 TGCCACCTGA CGTCTAAGAA ACCATTATTA TCATGACATT AACCTATAAA AATAGGCGTA 181 TCACGAGGCC CTTTCGTCTT CAAGAATTCT CATGTTTGAC AGCTTATCAT CGATAAGCTT 241 GGATCTGGAG CTGTAATATA AAAACCTTCT TCAACTAACG GGGCAGGTTA GTGACATTAG 301 AAAACCGACT GTAAAAAGTA CAGTCGGCAT TATCTCATAT TATAAAAGCC AGTCATTAGG 361 CCTATCTGAC AATTCCTGAA TAGAGTTCAT AAACAATCCT GCATGATAAC CATCACAAAC 421 AGAATGATGT ACCTGTAAAG ATAGCGGTAA ATATATTGAA TTACCTTTAT TAATGAATTT 481 TCCTGCTGTA ATAATGGGTA GAAGGTAATT ACTATTATTA TTGATATTTA AGTTAAACCC 541 AGTAAATGAA GTCCATGGAA TAATAGAAAG AGAAAAAGCA TTTTCAGGTA TAGGTGTTTT 601 GGGAAACAAT TTCCCCGAAC CATTATATTT CTCTACATCA GAAAGGTATA AATCATAAAA 661 CTCTTTGAAG TCATTCTTTA CAGGAGTCCA AATACCAGAG AATGTTTTAG ATACACCATC 721 AAAAATTGTA TAAAGTGGCT CTAACTTATC CCAATAACCT AACTCTCCGT CGCTATTGTA 781 ACCAGTTCTA AAAGCTGTAT TTGAGTTTAT CACCCTTGTC ACTAAGAAAA TAAATGCAGG 841 GTAAAATTTA TATCCTTCTT GTTTTATGTT TCGGGTATAA AACACTAATA TCAATTTTCT 901 GTGGTTATAC TAAAAGTCGT TTGTTGGGTT CAAATAATGA TTAAATATCT CTTTTCTCTT 961 CCAATTTGTC TAAATCAATT TTATTAAAGG TTCATTTGAT ATGCCTTCCT AAATTTTTAT 1021 CTAAAGTGAA ATTAGGAGGC TTACTTTGTC TGCTTTCTTC ATTAGAATCA ATCCTTTTTT 1081 AAAAGTCATA TTAACTGGTA ACGTAAATAT GTATTTTGAA AAATAATCCC AACTTTTTAT 1141 CCCAATTTTT TCGGTAATG // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
| original | Hirayama, Y., Development of a double-crossover markerless gene deletion system in Bifidobacterium longum: functional analysis of the α-galactosidase gene for raffinose assimilation. Appl. Environ. Microbiol. 78 (14): 4984-4994 (2012). PMID 22582061. |
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| reference | Nishiyama, K., Bifidobacterium bifidum Extracellular Sialidase Enhances Adhesion to the Mucosal Surface and Supports Carbohydrate Assimilation. MBio 8 (5). pii: e00928-17 (2017). PMID 28974612. |
| reference | Fukiya, S., Development of practical gene-mutagenesis systems in bifidobacteria. (Japanese text) Seibutsu-kogaku 94 (3): 110-116 (2016). |
2019.11.02
GNP_filter3_RDBDEP_html_190927.pl