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pcDNA3-hRUNX1b-9KR

Expression vector of human runt related transcription factor 1 isoform b (RUNX1b) with lysine-to-arginine substitutions.

Catalog number RDB15971
Resource name pcDNA3-hRUNX1b-9KR
Clone info. Expression vector of human runt related transcription factor 1 isoform b (RUNX1b), lysine-to-arginine substitutions (K24R, K43R, K83R, K90R, K125R, K144R, K167R, K182R, K188R), see Fig. 4A, B of Yonezawa, T. et al., J. Biol. Chem., 292 (30): 12528-12541, 2017.
Expression was confirmed by the depositor with western blot and immunofluorescent analysis.
Vector backbone pcDNA3.1 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E.coli), Neo^r (mammalian cells).
Gene/insert name human RUNX1 cDNA
Depositor Goyama, Susumu |
 
Other clones in our bank human RUNX1 (NCBI Gene 861) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Yonezawa, T et al. J. Biol. Chem. 292 (30) 12528-12541, 2017)
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RDB15971 pcDNA3-hRUNX1b-9KR Under QC test. Please contact us. DNA solution

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References and tips

Electronic file

Original reference

original Yonezawa, T., The ubiquitin ligase STUB1 regulates stability and activity of RUNX1 and RUNX1-RUNX1T1. J. Biol. Chem. 292 (30): 12528-12541 (2017). PMID 28536267.

Further references such as user reports and related articles (go to bottom)

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.

References

Original, user report and related articles

original Yonezawa, T., The ubiquitin ligase STUB1 regulates stability and activity of RUNX1 and RUNX1-RUNX1T1. J. Biol. Chem. 292 (30): 12528-12541 (2017). PMID 28536267.

2019.07.17

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