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pcDNA3-hRUNX1b-9KR (#RDB15971)

Expression vector of human runt related transcription factor 1 isoform b (RUNX1b) with lysine-to-arginine substitutions.

Clone info. Expression vector of human runt related transcription factor 1 isoform b (RUNX1b), lysine-to-arginine substitutions (K24R, K43R, K83R, K90R, K125R, K144R, K167R, K182R, K188R), see Fig. 4A, B of Yonezawa, T. et al., J. Biol. Chem., 292 (30): 12528-12541, 2017.
Comment Expression was confirmed by the depositor with western blot and immunofluorescent analysis.
Vector backbone pcDNA3.1 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E.coli), Neo^r (mammalian cells).
Gene/insert name human RUNX1 cDNA
Depositor|Developer Goyama, Susumu |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Yonezawa, T et al. J. Biol. Chem. 292 (30) 12528-12541, 2017)
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yonezawa, T et al. J. Biol. Chem. 292 (30): 12528-12541, 2017)。

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RDB15971 pcDNA3-hRUNX1b-9KR Under QC test. Please contact us. DNA solution

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How to cite this biological resource

Materials & Methods section:

The pcDNA3-hRUNX1b-9KR was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15971).

Reference section:

Yonezawa, T., Takahashi, H., Shikata, S., Liu, X., Tamura, M., Asada, S., Fukushima, T., Fukuyama, T., Tanaka, Y., Sawasaki, T., Kitamura, T., Goyama, S., The ubiquitin ligase STUB1 regulates stability and activity of RUNX1 and RUNX1-RUNX1T1. J. Biol. Chem. 292 (30): 12528-12541 (2017). PMID 28536267. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Yonezawa, T., The ubiquitin ligase STUB1 regulates stability and activity of RUNX1 and RUNX1-RUNX1T1. J. Biol. Chem. 292 (30): 12528-12541 (2017). PMID 28536267. [link to RRC of NBRP]