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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pHisGST CKI delta (#RDB15964)

Bacterial expression vector of mouse casein kinase 1, delta (CKI delta), residues 1-294.

Clone info. Bacterial expression vector of mouse casein kinase 1, delta (CKI delta), residues 1-294, fused with His-GST at N terminus.
Comment Expression was confirmed by the depositor.
Vector backbone pET-28a(+) (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Kan^r
Gene/insert name mouse Csnk1d cDNA
Depositor|Developer Ueda, Hiroki |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Shinohara, Y et al. Molecular Cell 67, 783–798, 2017)
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Shinohara, Y et al. Molecular Cell 67, 783–798, 2017)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15964 pHisGST CKI delta DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pHisGST CKI delta was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15964).

Reference section:

Shinohara, Y., Koyama, Y.M., Ukai-Tadenuma, M., Hirokawa, T., Kikuchi, M., Yamada, R.G., Ukai, H., Fujishima, H., Umehara, T., Tainaka, K., Ueda, H.R., Temperature-Sensitive Substrate and Product Binding Underlie Temperature-Compensated Phosphorylation in the Clock. Mol. Cell 67 (5): 783-798.e20 (2017). PMID 28886336. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15964_A8Ehp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: GST-M_F
Sequence file: RDB15964_A8Eha.seq check
>D05351A1_A8Eh_1_GST-M_F_A07_01_ABI24.ab1
    1 CGGATAGGCC TTTTGAACTC TCAAGTTGAT TTTCTTAGCA AGCTACCTGA AATGCTGAAA
   61 ATGTTCGAAG ATCGTTTATG TCATAAAACA TATTTAAATG GTGATCATGT AACCCATCCT
  121 GACTTCATGT TGTATGACGC TCTTGATGTT GTTTTATACA TGGACCCAAT GTGCCTGGAT
  181 GCGTTCCCAA AATTAGTTTG TTTTAAAAAA CGTATTGAAG CTATCCCACA AATTGATAAG
  241 TACTTGAAAT CCAGCAAGTA TATAGCATGG CCTTTGCAGG GCTGGCAAGC CACGTTTGGT
  301 GGTGGCGACC ATCCTCCAAA ATCGGATGGG TCTAGCGGCT CCAGCGAACA CCTGTATTTT
  361 CAGGGAGGCA TGGAGCTGAG GGTCGGGAAC AGGTACCGAC TGGGCCGCAA GATCGGCAGC
  421 GGCTCCTTCG GAGACATCTA TCTCGGTACG GACATTGCTG CGGGAGAAGA AGTTGCCATC
  481 AAGCTTGAAT GTGTCAAAAC CAAACATCCT CAGCTCCACA TTGAGAGCAA GATCTACAAA
  541 ATGATGCAGG GAGGAGTGGG CATCCCTACC ATCAGATGGT GTGGGGCTGA GGGGGACTAC
  601 AATGTCATGG TGATGGAGCT ACTGGGACCC AGCCTGGAAG ACCTGTTCAA CTTCTGTTCA
  661 AGGAAGTTTA GTCTCAAAAC TGTTCTGTTG CTTGCTGACC AAATGATAAG TCGTATTGAG
  721 TACATTCATT CGAAGAATTT TATCCACCGA GATGTGAAGC CAGATAACTT CCTCATGGGG
  781 CTGGGAAAGA AAGGCAACCT GGTCTACATC ATTGACTTTG GGCTGGCCAA GAAGTATCGG
  841 GATGCACGCA CCCACCAGCA TATCCCCTAT CGAGAGAACA AGAACCTCAC AGGGACAGCA
  901 CGCTATGCCT CCATCAACAC GCACCTTGGC ATTGAAACAA TCTCGAAAGG GATGACTTGG
  961 AGTCTCTGGG GTACGTGCTG ATGTACTTTC AACCTGCGCT CTCTCCCCTG GGCAAGGGCT
 1021 GCGACCGGCC ACCCAGAGGC CAGAGGTATG AGAGGATCAG TGAGAGAAGA TGGTCCACCT
1081 CCCAATTGAA GGTGGCTGAA TGGA
//
Primer: f1ori_R3
Sequence file: RDB15964_A8Ehb.seq check
>D05351A1_A8Eh_1_f1ori-R3_B07_04_ABI24.ab1
    1 CGCCATGCCT TCGGAATAGT TCTCCTTTCA GCAAAACCCC TCAAAGACCC GTTTAGAGGC
   61 CCCAAGGGGT TATGCTAGTT ATTGCTCAGC GGTGGCAGCA GCCAACTCAG CTTCCTTTCG
  121 GGCTTTGTTA GCAGCCGGAT CTCAGTGGTG GTGGTGGTGG TGCTCGAGTT ATTTGAGCAT
  181 GTTCCAGTCG AACACATAGT CGTAGGAGAA GCCTTGGCGA TGGAACAGAT TTCTGAAGAG
  241 CTGTCTCAGG TAGGAGTAGT CAGGTTTGTC ATCAAAACGT AAGGAACGGC AGAAATTCAG
  301 GTATGTGGCA AATTCAGAAG GATAGCCCTT GCACAGCACT TCAATGGGAG TGGACATCTT
  361 CTTCTCACTG ATCCTCTCAT ACTTCTGCCT CTTGGTGGCG GCCTTCAGCC CCTGCCAGGG
  421 GAGAGAGCCC AGGTTGAAGT ACATCAGCAC GTACCCCAGA GACTCCAAGT CATCCCTTCG
  481 AGATTGTTCA ATGCCAAGGT GCGTGTTGAT GGAGGCATAG CGTGCTGTCC CTGTGAGGTT
  541 CTTGTTCTCT CGATAGGGGA TATGCTGGTG GGTGCGTGCA TCCCGATACT TCTTGGCCAG
  601 CCCAAAGTCA ATGATGTAGA CCAGGTTGCC TTTCTTTCCC AGCCCCATGA GGAAGTTATC
  661 TGGCTTCACA TCTCGGTGGA TAAAATTCTT CGAATGAATG TACTCAATAC GACTTATCAT
  721 TTGGTCAGCA AGCAACAGAA CAGTTTTGAG ACTAAACTTC CTTGAACAGA AGTTGAACAG
  781 GTCTTCCAGG CTGGGTCCCA GTAGCTCCAT CACCATGACA TTGTAGTCCC CCTCAGCCCC
  841 ACACCATCTG ATGGTAGGGG ATGCCCACTC CTCCCTGCAT CATTTTTGTA GATCTTGCTC
  901 TCAATGTGGA GCTGAGGATG TTTGGTTTTG ACACATTCAA GCTTGATGGC AACTTCTTCT
  961 CCCGCAGCAA TGTCCGTACC GAGATAGATG TCTCCGAATG GAGCCGCTGC CGATCTTTGC
 1021 GGCCCAGTCG GGTACCTGTT TCCCGACCCT CAGCTCCATG CCTCCTGGAA ATACAGGTGA
1081 TTCGCTGGAA GTCTCGCCTA GGAAACCCCC CAT
//
Primer: GST-N_rev
Sequence file: RDB15964_A8Ehc.seq check
>D05351A1_A8Eh_1_GST-N_rev_C07_07_ABI24.ab1
    1 CTTCTTCAGG ATTTCCAAAG AGTCGAGTGG GTTGCACAAG GCCCTTAATT TTCCAATAAC
   61 CTAGTATAGG GGACATGGCA TGAGCGTGCT CATGTTTGTG ATGATTGTGG ATGAGATGAT
  121 CTTTGCCCAT GGTATATCTC CTTCTTAAAG TTAAACAAAA TTATTTCTAG AGGGGAATTG
  181 TTATCCGCTC ACAATTCCCC TATAGTGAGT CGTATTAATT TCGCGGGATC GAGATCTCGA
  241 TCCTCTACGC CGGACGCATC GTGGCCGGCA TCACCGGCGC CACAGGTGCG GTTGCTGGCG
  301 CCTATATCGC CGACATCACC GATGGGGAAG ATCGGGCTCG CCACTTCGGG CTCATGAGCG
  361 CTTGTTTCGG CGTGGGTATG GTGGCAGGCC CCGTGGCCGG GGGACTGTTG GGCGCCATCT
  421 CCTTGCATGC ACCATTCCTT GCGGCGGCGG TGCTCAACGG CCTCAACCTA CTACTGGGCT
  481 GCTTCCTAAT GCAGGAGTCG CATAAGGGAG AGCGTCGAGA TCCCGGACAC CATCGAATGG
  541 CGCAAAACCT TTCGCGGTAT GGCATGATAG CGCCCGGAAG AGAGTCAATT CAGGGTGGTG
  601 AATGTGAAAC CAGTAACGTT ATACGATGTC GCAGAGTATG CCGGTGTCTC TTATCAGACC
  661 GTTTCCCGCG TGGTGAACCA GGCCAGCCAC GTTTCTGCGA AAACGCGGGA AAAAGTGGAA
  721 GCGGCGATGG CGGAGCTGAA TTACATTCCC AACCGCGTGG CACAACAACT GGCGGGCAAA
  781 CAGTCGTTGC TGATTGGCGT TGCCACCTCC AGTCTGGCCC TGCACGCGCC GTCGCAAATT
  841 GTCGCGGCGA TTAAATCTCG CGCCGATCAA CTGGGTGCCA GCGTGGTGGT GTCGATGGTA
  901 GAACGAAGCG GCGTCGAAGC CTGTAAAGCG GCGGTGCACA ATCTTCTCGC GCAACGCGTC
  961 AGTGGGGCTG ATCATTAACT ATCCGCTGGA TGAACCAGGA TGCCATTGCT GTGGAAAGCT
 1021 GCCTGCACTA ATGTTCCGGC GATAATTTCA TGGAATGGTC TCTGAACAGA ACACCCCAAT
1081 TCGACAAGTA GTAATTTTTT CCTCCCCAAT GTG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Shinohara, Y., Temperature-Sensitive Substrate and Product Binding Underlie Temperature-Compensated Phosphorylation in the Clock. Mol. Cell 67 (5): 783-798.e20 (2017). PMID 28886336. [link to RRC of NBRP]