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p3xFLAG-C/EBP beta (K133R) (#RDB15963)

Expression vector of mouse CCAAT/enhancer-binding protein family members beta (C/EBP beta) with mutation at the SUMO acceptor site.

Clone info. Expression vector of mouse CCAAT/enhancer-binding protein family members beta (C/EBP beta) with mutation at the SUMO acceptor site (K133R), tagged with 3xFLAG at N terminus, CMV promoter.
Comment Myc-tag is not available. Expression was confirmed by the depositor with western blotting.
Vector backbone p3xFLAG-CMV 26 (plasmid)
Size of vector backbone 6.3 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name mouse Cebpb cDNA
Depositor|Developer Ashida, Hitoshi | Mitani, Takakazu |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Mitani, T et al. Biochim. Biophys. Acta. 1864(12): 2438-2448, 2017)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mitani, T et al. Biochim. Biophys. Acta. 1864(12): 2438-2448, 2017)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB15963 p3xFLAG-C/EBP beta (K133R) Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The p3xFLAG-C/EBP beta (K133R) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15963).

Reference section:

Mitani, T., Watanabe, S., Yoshioka, Y., Katayama, S., Nakamura, S., Ashida, H., Theobromine suppresses adipogenesis through enhancement of CCAAT-enhancer-binding protein beta degradation by adenosine receptor A1. Biochim. Biophys. Acta 1864 (12): 2438-2448 (2017). PMID 28965824. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Mitani, T., Theobromine suppresses adipogenesis through enhancement of CCAAT-enhancer-binding protein beta degradation by adenosine receptor A1. Biochim. Biophys. Acta 1864 (12): 2438-2448 (2017). PMID 28965824. [link to RRC of NBRP]