BL21(DE3)-based E. coli auxotroph host strain used for selective isotope labeling. Genotype: thrC ilvA;
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External Database Reference sequence |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Iwasaki lab auxotrophic host strains, unpublished). |
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Ordering | Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment ![]() ![]() Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word ![]() |
Remarks | It is recommended that colonies be isolated and cultured on an appropriate agar plate before performing your experiments. |
提供条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Iwasaki lab auxotrophic host strains, unpublished)。 |
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提供依頼 | 手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word ![]() 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word ![]() 遺伝子組換え生物の受入れ確認書 [Word ![]() |
備考 | 実験を行う前に、適切な寒天プレート上でコロニーを単離し、培養することを推奨します。 組換え体提供にかかる書式が必要です。 |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB15960 | EH1 | Under QC test. Please contact us. | Bacteria in stab agar |
Electronic file
Original reference
Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Escherichia coli auxotrophic expression strains (by the depositor) (English text) |
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Featured content | Bacteria host strains (en) (English text) |
Featured content | Bacteria host strains (ja) (Japanese text) |
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
reference | Iwasaki, T., Dissection of hydrogen bond interaction network around an iron-sulfur cluster by site-specific isotope labeling of hyperthermophilic archaeal Rieske-type ferredoxin. J. Am. Chem. Soc. 134 (48): 19731-19738 (2012). PMID 23145461. |
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reference | Lin, M.T., A rapid and robust method for selective isotope labeling of proteins. Methods 55 (4): 370-378 (2011). PMID 21925267. |
protocol | Lin, M.T., Escherichia coli auxotroph host strains for amino acid-selective isotope labeling of recombinant proteins. Methods Enzymol. 565: 45-66 (2015). PMID 26577727. |
2022.05.17
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