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RF21

BL21(DE3)-based E. coli auxotroph host strain used for selective isotope labeling. Genotype: aspC tyrB ilvE avtA yfbQ(alaA) yfdZ(alaC);

Catalog number RDB15959
Resource name RF21
Clone info. BL21(DE3)-based E. coli auxotroph host strain used for selective isotope labeling. Genotype: aspC tyrB ilvE avtA yfbQ(alaA) yfdZ(alaC); Amino acid requirement and/or labeling: Asp, Tyr, Phe, Ile, Leu, Val.
Comment Please refer web site of Escherichia coli auxotrophic expression strains (by the depositor), Lin et al., Methods 55 (4): 370-378 (2011), Iwasaki et al., J. Am. Chem. Soc. 134 (48): 19731-19738 (2012), and/or Lin et al., Methods Enzymol. 565: 45-66 (2015). All ML strains werre engineered by Dr. Gennis research group at University of Illinois at Urbana-Champaign, U.S.A., and YM, MS, EH, and RF strains were engineered by Dr. Iwasaki research group at Nippon Medical School, Japan. This work was supported in part by the Internaitonal Collaborations in Chemistry Grant from JSPS and NSF, the Nagase Science and Technology Foundation Research Grant, and JSPS grants-in-aid.
Vector backbone Bacteria culture
Growth remarks Please note the requirement of culture medium composition. It is recommended that colonies be isolated and cultured on an appropriate agar plate before performing your experiments.
Depositor|Developer Iwasaki, Toshio |
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            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Iwasaki lab auxotrophic host strains, unpublished).
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Remarks It is recommended that colonies be isolated and cultured on an appropriate agar plate before performing your experiments.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Iwasaki lab auxotrophic host strains, unpublished)。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            遺伝子組換え生物の受入れ確認書 [Word]
            備考 実験を行う前に、適切な寒天プレート上でコロニーを単離し、培養することを推奨します。
            組換え体提供にかかる書式が必要です。

            Catalog # Resource name Availability Shipping form Fee (non-profit org.)
            RDB15959 RF21 Under QC test. Please contact us. Bacteria in stab agar

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References and tips

            Electronic file

            Original reference

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Escherichia coli auxotrophic expression strains (by the depositor) (English text)
            Featured content Bacteria host strains (en) (English text)
            Featured content Bacteria host strains (ja) (Japanese text)

            Sequence information

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


            References

            Original, user report and related articles

            reference Iwasaki, T., Dissection of hydrogen bond interaction network around an iron-sulfur cluster by site-specific isotope labeling of hyperthermophilic archaeal Rieske-type ferredoxin. J. Am. Chem. Soc. 134 (48): 19731-19738 (2012). PMID 23145461.
            reference Lin, M.T., A rapid and robust method for selective isotope labeling of proteins. Methods 55 (4): 370-378 (2011). PMID 21925267.
            protocol Lin, M.T., Escherichia coli auxotroph host strains for amino acid-selective isotope labeling of recombinant proteins. Methods Enzymol. 565: 45-66 (2015). PMID 26577727.

            2022.05.17

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