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RF21

BL21(DE3)-based E. coli auxotroph host strain used for selective isotope labeling. Genotype: aspC tyrB ilvE avtA yfbQ(alaA) yfdZ(alaC);

Catalog number RDB15959
Resource name RF21
Clone info. BL21(DE3)-based E. coli auxotroph host strain used for selective isotope labeling. Genotype: aspC tyrB ilvE avtA yfbQ(alaA) yfdZ(alaC); Amino acid requirement and/or labeling: Asp, Tyr, Phe, Ile, Leu, Val.
Please refer web site of Escherichia coli auxotrophic expression strains (by the depositor), Lin et al., Methods 55 (4): 370-378 (2011), Iwasaki et al., J. Am. Chem. Soc. 134 (48): 19731-19738 (2012), and/or Lin et al., Methods Enzymol. 565: 45-66 (2015). All ML strains werre engineered by Dr. Gennis research group at University of Illinois at Urbana-Champaign, U.S.A., and YM, MS, EH, and RF strains were engineered by Dr. Iwasaki research group at Nippon Medical School, Japan. This work was supported in part by the Internaitonal Collaborations in Chemistry Grant from JSPS and NSF, the Nagase Science and Technology Foundation Research Grant, and JSPS grants-in-aid.
Vector backbone Bacteria culture
Depositor Iwasaki, Toshio |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Iwasaki lab auxotrophic host strains, unpublished).
(Japanese text [open/close])

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15959 RF21 Bacteria in stub agar

Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.

References and tips

Electronic file

Featured content

Featured content Escherichia coli auxotrophic expression strains (by the depositor) (English text)

References

reference Lin, M.T., Escherichia coli auxotroph host strains for amino acid-selective isotope labeling of recombinant proteins. Methods Enzymol. 565: 45-66 (2015). PMID 26577727.
reference Iwasaki, T., Dissection of hydrogen bond interaction network around an iron-sulfur cluster by site-specific isotope labeling of hyperthermophilic archaeal Rieske-type ferredoxin. J. Am. Chem. Soc. 134 (48): 19731-19738 (2012). PMID 23145461.
reference Lin, M.T., A rapid and robust method for selective isotope labeling of proteins. Methods 55 (4): 370-378 (2011). PMID 21925267.

2018.06.12

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