BL21(DE3)-based E. coli auxotroph host strain used for selective isotope labeling. Genotype: aspC tyrB ilvE avtA yfbQ(alaA) yfdZ(alaC);
|Clone info.||BL21(DE3)-based E. coli auxotroph host strain used for selective isotope labeling. Genotype: aspC tyrB ilvE avtA yfbQ(alaA) yfdZ(alaC); Amino acid requirement and/or labeling: Asp, Tyr, Phe, Ile, Leu, Val.
Please refer web site of Escherichia coli auxotrophic expression strains (by the depositor), Lin et al., Methods 55 (4): 370-378 (2011), Iwasaki et al., J. Am. Chem. Soc. 134 (48): 19731-19738 (2012), and/or Lin et al., Methods Enzymol. 565: 45-66 (2015). All ML strains werre engineered by Dr. Gennis research group at University of Illinois at Urbana-Champaign, U.S.A., and YM, MS, EH, and RF strains were engineered by Dr. Iwasaki research group at Nippon Medical School, Japan. This work was supported in part by the Internaitonal Collaborations in Chemistry Grant from JSPS and NSF, the Nagase Science and Technology Foundation Research Grant, and JSPS grants-in-aid.
|Vector backbone||Bacteria culture|
|Growth remarks||Please note the requirement of culture medium composition. It is recommended that colonies be isolated and cultured on an appropriate agar plate before performing your experiments.|
|Depositor|Developer||Iwasaki, Toshio ||
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
|Terms and conditions set forth by the DEPOSITOR||In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Iwasaki lab auxotrophic host strains, unpublished).|
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|Remarks||It is recommended that colonies be isolated and cultured on an appropriate agar plate before performing your experiments. |
|提供条件||利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Iwasaki lab auxotrophic host strains, unpublished)。|
|Catalog #||Resource name||Availability||Shipping form||Fee (non-profit org.)|
|RDB15959||RF21||Under QC test. Please contact us.||Bacteria in stab agar|
Further references such as user reports and related articles (go to bottom)
|Featured content||Escherichia coli auxotrophic expression strains (by the depositor) (English text)|
|Featured content||Bacteria host strains (en) (English text)|
|Featured content||Bacteria host strains (ja) (Japanese text)|
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
|reference||Iwasaki, T., Dissection of hydrogen bond interaction network around an iron-sulfur cluster by site-specific isotope labeling of hyperthermophilic archaeal Rieske-type ferredoxin. J. Am. Chem. Soc. 134 (48): 19731-19738 (2012). PMID 23145461.|
|reference||Lin, M.T., A rapid and robust method for selective isotope labeling of proteins. Methods 55 (4): 370-378 (2011). PMID 21925267.|
|protocol||Lin, M.T., Escherichia coli auxotroph host strains for amino acid-selective isotope labeling of recombinant proteins. Methods Enzymol. 565: 45-66 (2015). PMID 26577727.|