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RF8 (#RDB15950)

BL21(DE3)-based E. coli auxotroph host strain used for selective isotope labeling. Genotype: asnA asnB;

Clone info. BL21(DE3)-based E. coli auxotroph host strain used for selective isotope labeling. Genotype: asnA asnB; Amino acid requirement and/or labeling: Asn.
Comment Please refer web site of Escherichia coli auxotrophic expression strains (by the depositor), Lin et al., Methods 55 (4): 370-378 (2011), Iwasaki et al., J. Am. Chem. Soc. 134 (48): 19731-19738 (2012), and/or Lin et al., Methods Enzymol. 565: 45-66 (2015). All ML strains werre engineered by Dr. Gennis research group at University of Illinois at Urbana-Champaign, U.S.A., and YM, MS, EH, and RF strains were engineered by Dr. Iwasaki research group at Nippon Medical School, Japan. This work was supported in part by the Internaitonal Collaborations in Chemistry Grant from JSPS and NSF, the Nagase Science and Technology Foundation Research Grant, and JSPS grants-in-aid.
Vector backbone Bacteria culture
Growth remarks Please note the requirement of culture medium composition. It is recommended that colonies be isolated and cultured on an appropriate agar plate before performing your experiments.
Depositor|Developer Iwasaki, Toshio |
 

Distribution information

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (M.T. Lin et al., Methods Enzymol. 565, 45-66, 2015).
Additional terms and conditions:
Commercialization or third-party transfer of this strain is prohibited.
Remarks It is recommended that colonies be isolated and cultured on an appropriate agar plate before performing your experiments.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (M.T. Lin et al., Methods Enzymol. 565, 45-66, 2015)。
MTAに書く付加的使用条件：
菌株の商品化や第三者移転は禁止されている。
備考 実験を行う前に、適切な寒天プレート上でコロニーを単離し、培養することを推奨します。
組換え体提供にかかる書式が必要です。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB15950 RF8 Under QC test. Please contact us. Bacteria in stab agar

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The RF8 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15950).

Reference section:

Lin, M.T., Fukazawa, R., Miyajima-Nakano, Y., Matsushita, S., Choi, S.K., Iwasaki, T., Gennis, R.B., Escherichia coli auxotroph host strains for amino acid-selective isotope labeling of recombinant proteins. Methods Enzymol. 565: 45-66 (2015). PMID 26577727. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Lin, M.T., Escherichia coli auxotroph host strains for amino acid-selective isotope labeling of recombinant proteins. Methods Enzymol. 565: 45-66 (2015). PMID 26577727. [link to RRC of NBRP]
reference Iwasaki, T., Dissection of hydrogen bond interaction network around an iron-sulfur cluster by site-specific isotope labeling of hyperthermophilic archaeal Rieske-type ferredoxin. J. Am. Chem. Soc. 134 (48): 19731-19738 (2012). PMID 23145461. [link to RRC of NBRP]
reference Lin, M.T., A rapid and robust method for selective isotope labeling of proteins. Methods 55 (4): 370-378 (2011). PMID 21925267. [link to RRC of NBRP]