BL21(DE3)-based E. coli auxotroph host strain used for selective isotope labeling. Genotype: aspC tyrB hisG;
Alternative name | HG008 |
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Clone info. | BL21(DE3)-based E. coli auxotroph host strain used for selective isotope labeling. Genotype: aspC tyrB hisG; Amino acid requirement and/or labeling: Asp, Tyr, (Phe), His. |
Comment | Please refer web site of Escherichia coli auxotrophic expression strains (by the depositor), Lin et al., Methods 55 (4): 370-378 (2011), Iwasaki et al., J. Am. Chem. Soc. 134 (48): 19731-19738 (2012), and/or Lin et al., Methods Enzymol. 565: 45-66 (2015). All ML strains werre engineered by Dr. Gennis research group at University of Illinois at Urbana-Champaign, U.S.A., and YM, MS, EH, and RF strains were engineered by Dr. Iwasaki research group at Nippon Medical School, Japan. This work was supported in part by the Internaitonal Collaborations in Chemistry Grant from JSPS and NSF, the Nagase Science and Technology Foundation Research Grant, and JSPS grants-in-aid. |
Vector backbone | Bacteria culture |
Growth remarks | Please note the requirement of culture medium composition. It is recommended that colonies be isolated and cultured on an appropriate agar plate before performing your experiments. |
Depositor|Developer | Iwasaki, Toshio | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (M.T. Lin et al., Methods Enzymol. 565, 45-66, 2015). Additional terms and conditions: Commercialization or third-party transfer of this strain is prohibited. |
Remarks | It is recommended that colonies be isolated and cultured on an appropriate agar plate before performing your experiments. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (M.T. Lin et al., Methods Enzymol. 565, 45-66, 2015)。 MTAに書く付加的使用条件: 菌株の商品化や第三者移転は禁止されている。 |
備考 | 実験を行う前に、適切な寒天プレート上でコロニーを単離し、培養することを推奨します。 組換え体提供にかかる書式が必要です。 |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB15948 | RF5 | Under QC test. Please contact us. | Bacteria in stab agar |
Materials & Methods section:
The RF5 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15948). |
Reference section:
Lin, M.T., Fukazawa, R., Miyajima-Nakano, Y., Matsushita, S., Choi, S.K., Iwasaki, T., Gennis, R.B., Escherichia coli auxotroph host strains for amino acid-selective isotope labeling of recombinant proteins. Methods Enzymol. 565: 45-66 (2015). PMID 26577727. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
original | Lin, M.T., Escherichia coli auxotroph host strains for amino acid-selective isotope labeling of recombinant proteins. Methods Enzymol. 565: 45-66 (2015). PMID 26577727. [link to RRC of NBRP] |
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reference | Iwasaki, T., Dissection of hydrogen bond interaction network around an iron-sulfur cluster by site-specific isotope labeling of hyperthermophilic archaeal Rieske-type ferredoxin. J. Am. Chem. Soc. 134 (48): 19731-19738 (2012). PMID 23145461. [link to RRC of NBRP] |
reference | Lin, M.T., A rapid and robust method for selective isotope labeling of proteins. Methods 55 (4): 370-378 (2011). PMID 21925267. [link to RRC of NBRP] |
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