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ML21

C43(DE3)-based E. coli auxotroph host strain used for selective isotope labeling. Genotype: tyrA hisG;

Catalog number RDB15934
Resource name ML21
Clone info. C43(DE3)-based E. coli auxotroph host strain used for selective isotope labeling. Genotype: tyrA hisG; Amino acid requirement and/or labeling: (Tyr), His.
Comment Please refer web site of Escherichia coli auxotrophic expression strains (by the depositor), Lin et al., Methods 55 (4): 370-378 (2011), Iwasaki et al., J. Am. Chem. Soc. 134 (48): 19731-19738 (2012), and/or Lin et al., Methods Enzymol. 565: 45-66 (2015). All ML strains werre engineered by Dr. Gennis research group at University of Illinois at Urbana-Champaign, U.S.A., and YM, MS, EH, and RF strains were engineered by Dr. Iwasaki research group at Nippon Medical School, Japan. This work was supported in part by the Internaitonal Collaborations in Chemistry Grant from JSPS and NSF, the Nagase Science and Technology Foundation Research Grant, and JSPS grants-in-aid.
Vector backbone Bacteria culture
Growth remarks Please note the requirement of culture medium composition. It is recommended that colonies be isolated and cultured on an appropriate agar plate before performing your experiments.
Depositor|Developer Iwasaki, Toshio |
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            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (M.T. Lin et al., Methods 55, 370-378, 2011).
            Additional terms and conditions:
            Commercialization or third-party transfer of this strain is prohibited.
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            Remarks It is recommended that colonies be isolated and cultured on an appropriate agar plate before performing your experiments.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (M.T. Lin et al., Methods 55, 370-378, 2011)。
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            菌株の商品化や第三者移転は禁止されている。
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            遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
            手続きの概要は、「提供申込みについて[link]」をご覧ください。
            備考 実験を行う前に、適切な寒天プレート上でコロニーを単離し、培養することを推奨します。
            組換え体提供にかかる書式が必要です。

            Catalog # Resource name Availability Shipping form Fee (non-profit org.)
            RDB15934 ML21 Under QC test. Please contact us. Bacteria in stab agar

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            How to cite this biological resource

            Materials & Methods section:

            The ML21 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15934).

            Reference section:

            Lin, M.T., Sperling, L.J., Frericks Schmidt, H.L., Tang, M., Samoilova, R.I., Kumasaka, T., Iwasaki, T., Dikanov, S.A., Rienstra, C.M., Gennis, R.B., A rapid and robust method for selective isotope labeling of proteins. Methods 55 (4): 370-378 (2011). PMID 21925267. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)

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            Featured content

            Featured content Escherichia coli auxotrophic expression strains (by the depositor) (English text)
            Featured content Bacteria host strains (en) (English text)
            Featured content Bacteria host strains (ja) (Japanese text)

            QC test results

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References

            Original, user report and related articles

            original Lin, M.T., A rapid and robust method for selective isotope labeling of proteins. Methods 55 (4): 370-378 (2011). PMID 21925267. [link to RRC of NBRP]
            reference Iwasaki, T., Dissection of hydrogen bond interaction network around an iron-sulfur cluster by site-specific isotope labeling of hyperthermophilic archaeal Rieske-type ferredoxin. J. Am. Chem. Soc. 134 (48): 19731-19738 (2012). PMID 23145461. [link to RRC of NBRP]
            protocol Lin, M.T., Escherichia coli auxotroph host strains for amino acid-selective isotope labeling of recombinant proteins. Methods Enzymol. 565: 45-66 (2015). PMID 26577727. [link to RRC of NBRP]

            2023.10.26

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