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pQE-Lrp

Expression vector of Escherichia coli K-12 MG1655 leucine-responsive regulatory protein (Lrp).

Catalog number RDB15924
Resource name pQE-Lrp
Clone info. Expression vector of Escherichia coli K-12 MG1655 leucine-responsive regulatory protein (Lrp) tagged with 6xHis at N-terminus.
Expression was confirmed by the depositor with mobility shift assay.
Vector backbone Plasmid
Size of vector backbone 3.5 kb
Selectable markers Amp^r
Gene/insert name E. coli lrp genomic DNA
Depositor Yoneyama, Hiroshi |
 
Other clones in our bank E. coli lrp (KEGG orthology K03719) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Ihara, K., J. Biosci. Bioeng. 123 (4): 444-450 (2017)).
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RDB15924 pQE-Lrp DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Ihara, K., Expression of the alaE gene is positively regulated by the global regulator Lrp in response to intracellular accumulation of l-alanine in Escherichia coli. J. Biosci. Bioeng. 123 (4): 444-450 (2017). PMID 28057466.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Bacteria host strains (en) (English text)
Featured content Bacteria host strains (ja) (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15924_A8E8p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pJB8-Forward | Sequence: RDB15924_A8E8a.seq
>D05325A4-5_A8E8_pJB8-Forward_A01_01_ABI08.ab1
    1 CAAAATTTAT TTGCTTTGTG AGCGGATAAC AATTTCACAC AGAATTCATT AAAGAGGAGA
   61 AATTAACTAT GAGAGGATCG CATCACCATC ACCATCACGG ATCCGTAGAT AGCAAGAAGC
  121 GCCCTGGCAA AGATCTCGAC CGTATCGATC GTAACATTCT TAATGAGTTG CAAAAGGATG
  181 GGCGTATTTC TAACGTCGAG CTTTCTAAAC GTGTGGGACT TTCCCCAACG CCGTGCCTTG
  241 AGCGTGTGCG TCGGCTGGAA AGACAAGGGT TTATTCAGGG CTATACGGCG CTGCTTAACC
  301 CCCATTATCT GGATGCATCA CTTCTGGTAT TCGTTGAGAT TACTCTGAAT CGTGGCGCAC
  361 CGGATGTGTT TGAACAATTC AATACCGCTG TACAAAAACT TGAAGAAATT CAGGAGTGTC
  421 ATTTAGTATC CGGTGATTTC GACTACCTGT TGAAAACACG CGTGCCGGAT ATGTCAGCCT
  481 ACCGTAAGTT GCTGGGGGAA ACCCTGCTGC GTCTGCCTGG CGTCAATGAC ACACGGACAT
  541 ACGTTGTTAT GGAAGAAGTC AAGCAGAGTA ATCGTCTGGT TATTAAGACG CGCTAACACG
  601 GAAGCTTAAT TAGCTGAGCT TGGACTCCTG TTGATAGATC CAGTAATGAC CTCAGAACTC
  661 CATCTGGATT TGTTCAGAAC GCTCGGTTGC CGCCGGGCGT TTTTTATTGG TGAGAATCCA
  721 AGCTAGCTTG GCGAGATTTT CAGGAGCTAA GGAAGCTAAA ATGGAGAAAA AAATCACTGG
  781 ATATACCACC GTTGATATAT CCCAATGGCA TCGTAAAGAA CATTTTGAGG CATTTCAGTC
  841 AGTTGCTCAA TGTACCTATA ACCAGACCGT TCAGCTGGAT ATTACGGCCT TTTTTAAAGA
  901 CCGTAAAGAA AAATAAGCAC AAGTTTTATC CGGGCCTTTA TTCACATTCT TGCCCGCCTG
  961 ATGAATGCTC ATCCGGGAAT TTCGTATGGC AATGAAGACG GTGAGCTGGT GATATGGGGA
 1021 TAGTTGTTCA CCCCTTTATA CACCGATTTC ATGAGCAAAC TGAAACGGTT TTCAATCGCT
1081 CTGGGAAGTG ATTACCCAAC GGAACGGATT TATTCC
//
Primer: Cm_N_rev | Sequence: RDB15924_A8E8b.seq
>D05325A4-5_A8E8_Cm_N_rev_B01_02_ABI08.ab1
    1 CGGGTCCCCA AAATGGTTCT TTACGATGCC ATTGGGATAT ATCAACGGTG GTATATCCAG
   61 TGATTTTTTT CTCCATTTTA GCTTCCTTAG CTCCTGAAAA TCTCGCCAAG CTAGCTTGGA
  121 TTCTCACCAA TAAAAAACGC CCGGCGGCAA CCGAGCGTTC TGAACAAATC CAGATGGAGT
  181 TCTGAGGTCA TTACTGGATC TATCAACAGG AGTCCAAGCT CAGCTAATTA AGCTTCCGTG
  241 TTAGCGCGTC TTAATAACCA GACGATTACT CTGCTTGACT TCTTCCATAA CAACGTATGT
  301 CCGTGTGTCA TTGACGCCAG GCAGACGCAG CAGGGTTTCC CCCAGCAACT TACGGTAGGC
  361 TGACATATCC GGCACGCGTG TTTTCAACAG GTAGTCGAAA TCACCGGATA CTAAATGACA
  421 CTCCTGAATT TCTTCAAGTT TTTGTACAGC GGTATTGAAT TGTTCAAACA CATCCGGTGC
  481 GCCACGATTC AGAGTAATCT CAACGAATAC CAGAAGTGAT GCATCCAGAT AATGGGGGTT
  541 AAGCAGCGCC GTATAGCCCT GAATAAACCC TTGTCTTTCC AGCCGACGCA CACGCTCAAG
  601 GCACGGCGTT GGGGAAAGTC CCACACGTTT AGAAAGCTCG ACGTTAGAAA TACGCCCATC
  661 CTTTTGCAAC TCATTAAGAA TGTTACGATC GATACGGTCG AGATCTTTGC CAGGGCGCTT
  721 CTTGCTATCT ACGGATCCGT GATGGTGATG GTGATGCGAT CCTCTCATAG TTAATTTCTC
  781 CTCTTTAATG AATTCTGTGT GAAATTGTTA TCCGCTCACA AAGCAAATAA ATTTTTTATG
  841 ATTTCTCGAG GTGAAGACGA AAAGGGCCTC GTGATACGCC CTATTTTTTA TAGGTTAATG
  901 TCATGATAAT TAATGGGTTT CTTAGAACGT CAGGTGGCAC TTTTTCGGGG GAAATGTGCC
  961 GCGGAACCCC CTATTTGTTT ATTTTTTCTA AATACATTCC AAATATGTAT CCGCTCATGA
 1021 GACCAATAAC CCCTGATTAA ATGCCTTCCA ATAATATTGG AAAAAGGGAG AAGTATGAGG
1081 TATTCAACAT TTTCCGTGTT CGCCCCTTTA ATTTTCCCCT GATGTTTATA GCGCG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Ihara, K., Expression of the alaE gene is positively regulated by the global regulator Lrp in response to intracellular accumulation of l-alanine in Escherichia coli. J. Biosci. Bioeng. 123 (4): 444-450 (2017). PMID 28057466.

2019.11.02

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