Expression vector of Escherichia coli K-12 MG1655 leucine-responsive regulatory protein (Lrp).
Clone info. | Expression vector of Escherichia coli K-12 MG1655 leucine-responsive regulatory protein (Lrp) tagged with 6xHis at N-terminus. |
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Comment | Expression was confirmed by the depositor with mobility shift assay. |
Vector backbone | Plasmid |
Size of vector backbone | 3.5 kb |
Selectable markers | Amp^r |
Gene/insert name | E. coli lrp genomic DNA |
Depositor|Developer | Yoneyama, Hiroshi | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Ihara, K., J. Biosci. Bioeng. 123 (4): 444-450 (2017)). |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Ihara, K., J. Biosci. Bioeng. 123 (4): 444-450 (2017))。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB15924 | pQE-Lrp | DNA solution |
Materials & Methods section:
The pQE-Lrp was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15924). |
Reference section:
Ihara, K., Sato, K., Hori, H., Makino, Y., Shigenobu, S., Ando, T., Isogai, E., Yoneyama, H., Expression of the alaE gene is positively regulated by the global regulator Lrp in response to intracellular accumulation of l-alanine in Escherichia coli. J. Biosci. Bioeng. 123 (4): 444-450 (2017). PMID 28057466. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB15924_A8E8p1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
Primer: pJB8-Forward Sequence file: RDB15924_A8E8a.seq |
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>D05325A4-5_A8E8_pJB8-Forward_A01_01_ABI08.ab1 1 CAAAATTTAT TTGCTTTGTG AGCGGATAAC AATTTCACAC AGAATTCATT AAAGAGGAGA 61 AATTAACTAT GAGAGGATCG CATCACCATC ACCATCACGG ATCCGTAGAT AGCAAGAAGC 121 GCCCTGGCAA AGATCTCGAC CGTATCGATC GTAACATTCT TAATGAGTTG CAAAAGGATG 181 GGCGTATTTC TAACGTCGAG CTTTCTAAAC GTGTGGGACT TTCCCCAACG CCGTGCCTTG 241 AGCGTGTGCG TCGGCTGGAA AGACAAGGGT TTATTCAGGG CTATACGGCG CTGCTTAACC 301 CCCATTATCT GGATGCATCA CTTCTGGTAT TCGTTGAGAT TACTCTGAAT CGTGGCGCAC 361 CGGATGTGTT TGAACAATTC AATACCGCTG TACAAAAACT TGAAGAAATT CAGGAGTGTC 421 ATTTAGTATC CGGTGATTTC GACTACCTGT TGAAAACACG CGTGCCGGAT ATGTCAGCCT 481 ACCGTAAGTT GCTGGGGGAA ACCCTGCTGC GTCTGCCTGG CGTCAATGAC ACACGGACAT 541 ACGTTGTTAT GGAAGAAGTC AAGCAGAGTA ATCGTCTGGT TATTAAGACG CGCTAACACG 601 GAAGCTTAAT TAGCTGAGCT TGGACTCCTG TTGATAGATC CAGTAATGAC CTCAGAACTC 661 CATCTGGATT TGTTCAGAAC GCTCGGTTGC CGCCGGGCGT TTTTTATTGG TGAGAATCCA 721 AGCTAGCTTG GCGAGATTTT CAGGAGCTAA GGAAGCTAAA ATGGAGAAAA AAATCACTGG 781 ATATACCACC GTTGATATAT CCCAATGGCA TCGTAAAGAA CATTTTGAGG CATTTCAGTC 841 AGTTGCTCAA TGTACCTATA ACCAGACCGT TCAGCTGGAT ATTACGGCCT TTTTTAAAGA 901 CCGTAAAGAA AAATAAGCAC AAGTTTTATC CGGGCCTTTA TTCACATTCT TGCCCGCCTG 961 ATGAATGCTC ATCCGGGAAT TTCGTATGGC AATGAAGACG GTGAGCTGGT GATATGGGGA 1021 TAGTTGTTCA CCCCTTTATA CACCGATTTC ATGAGCAAAC TGAAACGGTT TTCAATCGCT 1081 CTGGGAAGTG ATTACCCAAC GGAACGGATT TATTCC // |
Primer: Cm_N_rev Sequence file: RDB15924_A8E8b.seq |
>D05325A4-5_A8E8_Cm_N_rev_B01_02_ABI08.ab1 1 CGGGTCCCCA AAATGGTTCT TTACGATGCC ATTGGGATAT ATCAACGGTG GTATATCCAG 61 TGATTTTTTT CTCCATTTTA GCTTCCTTAG CTCCTGAAAA TCTCGCCAAG CTAGCTTGGA 121 TTCTCACCAA TAAAAAACGC CCGGCGGCAA CCGAGCGTTC TGAACAAATC CAGATGGAGT 181 TCTGAGGTCA TTACTGGATC TATCAACAGG AGTCCAAGCT CAGCTAATTA AGCTTCCGTG 241 TTAGCGCGTC TTAATAACCA GACGATTACT CTGCTTGACT TCTTCCATAA CAACGTATGT 301 CCGTGTGTCA TTGACGCCAG GCAGACGCAG CAGGGTTTCC CCCAGCAACT TACGGTAGGC 361 TGACATATCC GGCACGCGTG TTTTCAACAG GTAGTCGAAA TCACCGGATA CTAAATGACA 421 CTCCTGAATT TCTTCAAGTT TTTGTACAGC GGTATTGAAT TGTTCAAACA CATCCGGTGC 481 GCCACGATTC AGAGTAATCT CAACGAATAC CAGAAGTGAT GCATCCAGAT AATGGGGGTT 541 AAGCAGCGCC GTATAGCCCT GAATAAACCC TTGTCTTTCC AGCCGACGCA CACGCTCAAG 601 GCACGGCGTT GGGGAAAGTC CCACACGTTT AGAAAGCTCG ACGTTAGAAA TACGCCCATC 661 CTTTTGCAAC TCATTAAGAA TGTTACGATC GATACGGTCG AGATCTTTGC CAGGGCGCTT 721 CTTGCTATCT ACGGATCCGT GATGGTGATG GTGATGCGAT CCTCTCATAG TTAATTTCTC 781 CTCTTTAATG AATTCTGTGT GAAATTGTTA TCCGCTCACA AAGCAAATAA ATTTTTTATG 841 ATTTCTCGAG GTGAAGACGA AAAGGGCCTC GTGATACGCC CTATTTTTTA TAGGTTAATG 901 TCATGATAAT TAATGGGTTT CTTAGAACGT CAGGTGGCAC TTTTTCGGGG GAAATGTGCC 961 GCGGAACCCC CTATTTGTTT ATTTTTTCTA AATACATTCC AAATATGTAT CCGCTCATGA 1021 GACCAATAAC CCCTGATTAA ATGCCTTCCA ATAATATTGG AAAAAGGGAG AAGTATGAGG 1081 TATTCAACAT TTTCCGTGTT CGCCCCTTTA ATTTTCCCCT GATGTTTATA GCGCG // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Ihara, K., Expression of the alaE gene is positively regulated by the global regulator Lrp in response to intracellular accumulation of l-alanine in Escherichia coli. J. Biosci. Bioeng. 123 (4): 444-450 (2017). PMID 28057466. [link to RRC of NBRP] |
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