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MG1655 delta lacZ

Escherichia coli host strain. MG1655 with a deletion in lacZ.

Catalog number RDB15922
Resource name MG1655 delta lacZ
Clone info. Escherichia coli host strain. MG1655 with a deletion in lacZ. Resistance: kanamycin
Vector backbone Bacteria culture
Selectable markers kan^r
Growth remarks It is recommended that colonies be isolated and cultured on an appropriate agar plate before performing your experiments.
Gene/insert name E. coli lacZ genomic DNA
Depositor|Developer Yoneyama, Hiroshi |
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External Database
E. coli lacZ
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Hori, H et al. Appl. Environ. Microbiol. 77(12): 4027-2034, 2011)
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks It is recommended that colonies be isolated and cultured on an appropriate agar plate before performing your experiments.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hori, H et al. Appl. Environ. Microbiol. 77(12): 4027-2034, 2011)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
          備考 実験を行う前に、適切な寒天プレート上でコロニーを単離し、培養することを推奨します。
          組換え体提供にかかる書式が必要です。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB15922 MG1655 delta lacZ Under QC test. Please contact us. Bacteria in stab agar

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          How to cite this biological resource

          Materials & Methods section:

          The MG1655 delta lacZ was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15922).

          Reference section:

          Ihara, K., Sato, K., Hori, H., Makino, Y., Shigenobu, S., Ando, T., Isogai, E., Yoneyama, H., Expression of the alaE gene is positively regulated by the global regulator Lrp in response to intracellular accumulation of l-alanine in Escherichia coli. J. Biosci. Bioeng. 123 (4): 444-450 (2017). PMID 28057466.

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          Featured content Bacteria host strains (en) (English text)
          Featured content Bacteria host strains (ja) (Japanese text)

          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Ihara, K., Expression of the alaE gene is positively regulated by the global regulator Lrp in response to intracellular accumulation of l-alanine in Escherichia coli. J. Biosci. Bioeng. 123 (4): 444-450 (2017). PMID 28057466.

          2023.05.12

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