Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

MG1655 delta lrp (#RDB15921)

Escherichia coli host strain. MG1655 with a deletion in lrp gene.

Clone info. Escherichia coli host strain. MG1655 with a deletion in lrp gene. Resistance: not applicable (none)
Vector backbone Bacteria culture
Selectable markers no
Growth conditions LB, 37ºC
Growth remarks It is recommended that colonies be isolated and cultured on an appropriate agar plate before performing your experiments.
Gene/insert name E. coli lrp genomic DNA
Depositor|Developer Yoneyama, Hiroshi |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Hori, H et al. Appl. Environ. Microbiol. 77(12): 4027-2034, 2011).
Remarks It is recommended that colonies be isolated and cultured on an appropriate agar plate before performing your experiments.
提供案内 (日本国内) [open/close]

必要書類
遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hori, H et al. Appl. Environ. Microbiol. 77(12): 4027-2034, 2011)。
備考 実験を行う前に、適切な寒天プレート上でコロニーを単離し、培養することを推奨します。
組換え体提供にかかる書式が必要です。

Catalog # Resource name Shipping form Fee
RDB15921 MG1655 delta lrp Bacteria in stab agar

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The MG1655 delta lrp was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15921).

Reference section:

Ihara, K., Sato, K., Hori, H., Makino, Y., Shigenobu, S., Ando, T., Isogai, E., Yoneyama, H., Expression of the alaE gene is positively regulated by the global regulator Lrp in response to intracellular accumulation of l-alanine in Escherichia coli. J. Biosci. Bioeng. 123 (4): 444-450 (2017). PMID 28057466. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet Data Sheet open check

Nucleotide sequence of a portion of this resource (if available).

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ihara, K., Expression of the alaE gene is positively regulated by the global regulator Lrp in response to intracellular accumulation of l-alanine in Escherichia coli. J. Biosci. Bioeng. 123 (4): 444-450 (2017). PMID 28057466. [PubMed] [Article] [RRC of NBRP]