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pET-pfu-FAU-1-His (#RDB15910)

Bacterial expression vector of P. furiosus AU binding 1 FAU-1.

Clone info. Bacterial expression vector of P. furiosus AU binding 1 (FAU-1) tagged with 6xHis at C terminal. For the constitution and cloning, see Fig.5A of Ikeda, Y. et al., Sci. Rep., 7 (1):12674, 2017, and Kanai Aet al., Biochem. J. 372 (Pt 1): 253-261. 2003, respectively.
Comment Expression was confirmed by the depositor with SDS/PAGE. See also the following paper: Expression cloning and characterization of a novel gene that encodes the RNA-binding protein FAU-1 from Pyrococcus furiosus. Kanai A, Oida H, Matsuura N, Doi H. Biochem J. 372(Pt 1):253-261. 2003.
Vector backbone pET23b (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers kan^r
Gene/insert name P. furiosus FAU-1 genomic DNA
Reference of insert sequence AB055587 (DDBJ accession number)
Depositor|Developer Kanai, Akio |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ikeda, Y et al. Biochemical Journal 372: 253-261, 2003)
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ikeda, Y et al. Biochemical Journal 372: 253-261, 2003)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15910 pET-pfu-FAU-1-His DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pET-pfu-FAU-1-His was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15910).

Reference section:

Ikeda, Y., Okada, Y., Sato, A., Kanai, T., Tomita, M., Atomi, H., Kanai, A., An archaeal RNA binding protein, FAU-1, is a novel ribonuclease related to rRNA stability in Pyrococcus and Thermococcus. Sci. Rep. 7 (1): 12674 (2017). PMID 28978920. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15910_A8Dnp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: T7 terminator
Sequence file: RDB15910_A8Dna.seq check
>D05496A1-5_A8Dn_T7terminater_H01_22_ABI24.ab1
    1 AATTAACCTT CCTTTTCGGG CTTTGTTAGC AGCCGGATCT CAGTGGTGGT GGTGGTGGTG
   61 CTCGAGCCTG TCGTAGACCT CCTGGGCAAT CCTCAGCGTT GCCTTATAGA GCTTCTCACT
  121 AATAATTCCC TCCTCGTAGT GTTCCTTTAG CTTCTCCTTA TCTATGATCT CCTTCTTTCC
  181 GTCTGGCCAC CTCACTATGT CGACCTCTAG ATCCACATAT CTTGCCTTAT CTGGATAGAT
  241 CTCCACAGGG GTGTTGATGT TGTAGAACTC TCCCTTAAGG TTGCCATCTT TATCATAGTA
  301 TCTGTGAACA AACCACCACT TTCCAGCCTC TATTTCAGTT ATTGCGTAAT CTCCACTCTC
  361 TATTGGCACT TCCAGTCCAT CATAGAACTT TCCTGGCCTT AGATATCTCT TTATTGTCAC
  421 TTTTAAAGGA TCCGTGGATA CCTCTATTAC TTCCCCTGGG CCAATCTTTA TTATCTGCCC
  481 ATCAGGTTTC ACGTGGTTCA GCGTGAATAT CCAACCAACC TTTGGTCCTT TACTGGTAAT
  541 TATAGCTTCT AGAAATCCTT TACTTATCTT CTGCCTCTGG GACGGGAGTT TTGCCAGAAT
  601 CCCCTCTGCG ACATCTACAG CTAATGTAAA CTCTGGGTCG TAGGACTTGA ACTGGTGGTG
  661 CCCTTCAATT GTGGGAACAA CTTCATTCCT AATCTCATCA AGCTTTTTCT TGACTCCACC
  721 ACCGAATTCA ATCTCGTAAA TTTCCCTCCC CTCTATTATC TCTGCTGGAG CTGAGAACTT
  781 CTCTGCCTCC TTCAGCTTAT CTGCAATTTT TGAAAGCCTC ACTAGTTCAT CCCTTAACGT
  841 GTTCCAATCC TTGTAAGCGG CAGCAGTTCT CCAGAGAACA CCCCACTCTC CAAGATCAAC
  901 ACTTAGTCCC AGGATTCTCA ACCTTTCCCT TTCCTCGGAT CCTTTATTCT TTTCTGGGAA
  961 ATCTTCACGT GTCTCTGAAC TTCCTAATGG CTATGGAAAT CAGCACTGCA TAAGTCACCC
 1021 GGGAATTGTT ATTAAGTTGC TTAAGGTGTT GGGGAGGACG TTGTGCTTCT TCACTTTGGA
 1081 CAACCAACTT TCAATCCTTC GGCCAGCATT CTGGAAGGCT TCTCTATGGA GACGTTCGAT
1141 AACATTCCGA TATATCTACA TAACCAAAGC G
//
Primer: T7_20bp
Sequence file: RDB15910_A8Dnb.seq check
>D05496A1-5_A8Dn_T7_20bp_A02_02_ABI24.ab1
    1 GGAAATTTCA TCTAGAATAT TTTGTTTACT TTAAGAAGGA GATATACATA CATATGGTGA
   61 AAGTGTCTAC AGAATCAGAA ATAGCGGTGA GGATAAGGGG AATATATAGC ACGGCCCTAA
  121 CAAAGCTCCT AATGGACAGG GGTTTCAAGA TAGTACAGCC AAGCGACGTG ATAGCTGAGA
  181 GGTTTGGAAT TGAGAAGAGC TATGAGGACT TTGACGTCGA CATCTACGAC AAAAACCACG
  241 GTGTGACAAT CGTTGGAACC AAAGTGGAGG CGGTGAAGAA GGTCTTTGAG GAGGAGTTTA
  301 TTGACGTCTT TTTTAGAAAG CTCCCCTATA AGCTCCATGG AATTTACAAG GGGCTTGTGG
  361 TGAAAAGAGA TGACAGGTTT GTTTATGTAG ATATTGGGAA TGTTATTGGA ACGGTTCTCA
  421 TAGAGGAGCT TCCAGATGCT GCCGAAGGGG ATGAAGTTGT TGTTCAAGTG AAGAAGCACA
  481 ACGTCCTCCC ACACCTAAGC ACCTTAATAA CAATCCCGGG TGACTATGCA GTGCTGATTC
  541 CAAAGCCCAT AGGAGTTCAG AGACACGTGA AGATTTCCAG AAAGATAAAG GATCCCGAGG
  601 AAAGGGAAAG GTTGAGAATC CTGGGACTAA GTGTTGATCT TGGAGAGTGG GGTGTTCTCT
  661 GGAGAACTGC TGCCGCTTAC AAGGATTGGA ACACGTTAAG GGATGAACTA GTGAGGCTTT
  721 CAAAAATTGC AGATAAGCTG AAGGAGGCAG AGAAGTTCTC AGCTCCAGCA GAGATAATAG
  781 AGGGGAGGGA AATTTACGAG ATTGAATTCG GTGGTGGAGT CAAGAAAAAG CTTGATGAGA
  841 TTAGGAATGA AGTTGTTCCC ACAATTGAAG GGCACCACCA GTTCAAGTCC TACGACCCAG
  901 AGTTTACATT AGCTGTAGAT GTCGCAGAGG GGATTCTGGC AAAACTCCCG TCCCAGAGGC
  961 AGAAGATAAG TAAAGGATTT CTAGAAGCTA TAATTACCCA GTAAAGGACC AAAAGGTTGG
 1021 GTTGGGATAT TCACGCTGAA CCACGTGAAA CCTGATGGGC AGATATAAGA TTGGCCCAGG
 1081 GCAGTATAGA GTATCACCGA TCCTTTAAGT GACCATTAAG AGAATATCTA CGCCAGGCAA
1141 AAGGTTTCCT TATGAATTGG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ikeda, Y., An archaeal RNA binding protein, FAU-1, is a novel ribonuclease related to rRNA stability in Pyrococcus and Thermococcus. Sci. Rep. 7 (1): 12674 (2017). PMID 28978920. [link to RRC of NBRP]
reference Kanai, A., Expression cloning and characterization of a novel gene that encodes the RNA-binding protein FAU-1 from Pyrococcus furiosus. Biochem. J. 372 (Pt 1): 253-261 (2003). PMID 12614195.