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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pX330-3BP2 gRNA

Targeting construct of human c-Abl SH3 domain-binding protein-2 (3BP2).

Catalog number RDB15907
Resource name pX330-3BP2 gRNA
Clone info. Expression vector of gRNA for targeting human c-Abl SH3 domain-binding protein-2 (3BP2) and human codon-optimized SpCas9.
Comment Effect of the targeting vecctor was confirmed by the depositor with immunoblotting.
Vector backbone pX330 (plasmid)
Size of vector backbone 8.5 kb
Selectable markers Amp^r
Gene/insert name Human SH3BP2 genomic DNA
Depositor|Developer Sada, Kiyonao |
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External Database
Human SH3BP2

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Chihara, K et al. Sci Rep. 7(1) : 11480, 2017)
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          Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
          Remarks
          The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Chihara, K et al. Sci Rep. 7(1) : 11480, 2017)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          専用MTA(CRISPR/Cas9内包遺伝子材料専用 非営利学術目的)をお使いください [Word]
          備考
          本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15907 pX330-3BP2 gRNA DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Chihara, K., Syk-dependent tyrosine phosphorylation of 3BP2 is required for optimal FcRγ-mediated phagocytosis and chemokine expression in U937 cells. Sci. Rep. 7 (1): 11480 (2017). PMID 28904407.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15907_A8Crp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: SV40pA3' element Reverse
          Sequence file: RDB15907_A8Cra.seq check
          >D05305A5-1_A8Cr_40pA3'elementRever_E02_14_ABI24.ab1
              1 TGTCTAGGGG CGGACTATGG AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT
             61 TGCTGGCCTT TTGCTCACAT GTGAGGGCCT ATTTCCCATG ATTCCTTCAT ATTTGCATAT
            121 ACGATACAAG GCTGTTAGAG AGATAATTGG AATTAATTTG ACTGTAAACA CAAAGATATT
            181 AGTACAAAAT ACGTGACGTA GAAAGTAATA ATTTCTTGGG TAGTTTGCAG TTTTAAAATT
            241 ATGTTTTAAA ATGGACTATC ATATGCTTAC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
            301 TTTATATATC TTGTGGAAAG GACGAAACAC CGGGTACCGC CCTTCTTGTG CGTTTTAGAG
            361 CTAGAAATAG CAAGTTAAAA TAAGGCTAGT CCGTTATCAA CTTGAAAAAG TGGCACCGAG
            421 TCGGTGCTTT TTTGTTTTAG AGCTAGAAAT AGCAAGTTAA AATAAGGCTA GTCCGTTTTT
            481 AGCGCGTGCG CCAATTCTGC AGACAAATGG CTCTAGAGGT ACCCGTTACA TAACTTACGG
            541 TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAGTAACGC
            601 CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG
            661 CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT
            721 GGCCCGCCTG GCATTGTGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA
            781 TCTACGTATT AGTCATCGCT ATTACCATGG TCGAGGTGAG CCCCACGTTC TGCTTCACTC
            841 TCCCCATCTC CCCCCCCCTC CCCACCCCCC AATTTTGTAT TTATTTATTT TTTTAATTAT
          901 TTTTGTGCAG CGATGGGGGG CGCGGGGGGG GGGATC
          //
          Primer: RDB13535#1
          Sequence file: RDB15907_A8Crb.seq check
          >D05305A5-1_A8Cr_RDB13535#1_F02_17_ABI24.ab1
              1 TGGACATGCC TGAATCACTT TTTTTCAGGT TGGACCGGTG CCACCATGGA CTATAAGGAC
             61 CACGACGGAG ACTACAAGGA TCATGATATT GATTACAAAG ACGATGACGA TAAGATGGCC
            121 CCAAAGAAGA AGCGGAAGGT CGGTATCCAC GGAGTCCCAG CAGCCGACAA GAAGTACAGC
            181 ATCGGCCTGG ACATCGGCAC CAACTCTGTG GGCTGGGCCG TGATCACCGA CGAGTACAAG
            241 GTGCCCAGCA AGAAATTCAA GGTGCTGGGC AACACCGACC GGCACAGCAT CAAGAAGAAC
            301 CTGATCGGAG CCCTGCTGTT CGACAGCGGC GAAACAGCCG AGGCCACCCG GCTGAAGAGA
            361 ACCGCCAGAA GAAGATACAC CAGACGGAAG AACCGGATCT GCTATCTGCA AGAGATCTTC
            421 AGCAACGAGA TGGCCAAGGT GGACGACAGC TTCTTCCACA GACTGGAAGA GTCCTTCCTG
            481 GTGGAAGAGG ATAAGAAGCA CGAGCGGCAC CCCATCTTCG GCAACATCGT GGACGAGGTG
            541 GCCTACCACG AGAAGTACCC CACCATCTAC CACCTGAGAA AGAAACTGGT GGACAGCACC
            601 GACAAGGCCG ACCTGCGGCT GATCTATCTG GCCCTGGCCC ACATGATCAA GTTCCGGGGC
            661 CACTTCCTGA TCGAGGGCGA CCTGAACCCC GACAACAGCG ACGTGGACAA GCTGTTCATC
            721 CAGCTGGTGC AGACCTACAA CCAGCTGTTC GAGGAAAACC CCATCAACGC CAGCGGCGTG
            781 GACGCCAAGG CCATCCTGTC TGCCAGACTG AGCAAGAGCA GACGGCTGGA AAATCTGATC
            841 GCCCAGCTGC CCGGCGAGAA GAAGAATGGC CTGTTCGGAA ACCTGATTGC CCTGAGCCTG
            901 GGCCTGACCC CCCAACTTCA AGAGCAACTT CGACCTGGCC CGAGGGATGC CAAACTGCAG
            961 CTGAGCAAAG GACACCCTAC GACGACGACC CTGCACACCC TGCTGGCCCA GATCGGCCGA
           1021 CCCAGTACGC CCGAACCTGA TTTCTGGGCC CGCCCAAGAT CTGGTCGGAA CGTCCATTCC
          1081 TTGCTGAGCG ACCATTTC
          //
          Primer: BGH_rev
          Sequence file: RDB15907_A8Crc.seq check
          >D05305A5-1_A8Cr_BGH_rev2_G02_20_ABI24.ab1
              1 CTCTGGGGTA AGGATGCACG GGGGAGGGGC AAACAACAGA TGGCTGGCAA CTAGAAGGCA
             61 CAGTCGAGGC TGATCAGCGA GCTCTAGGAA TTCTTACTTT TTCTTTTTTG CCTGGCCGGC
            121 CTTTTTCGTG GCCGCCGGCC TTTTGTCGCC TCCCAGCTGA GACAGGTCGA TCCGTGTCTC
            181 GTACAGGCCG GTGATGCTCT GGTGGATCAG GGTGGCGTCC AGCACCTCTT TGGTGCTGGT
            241 GTACCTCTTC CGGTCGATGG TGGTGTCAAA GTACTTGAAG GCGGCAGGGG CTCCCAGATT
            301 GGTCAGGGTA AACAGGTGGA TGATATTCTC GGCCTGCTCT CTGATGGGCT TATCCCGGTG
            361 CTTGTTGTAG GCGGACAGCA CTTTGTCCAG ATTAGCGTCG GCCAGGATCA CTCTCTTGGA
            421 GAACTCGCTG ATCTGCTCGA TGATCTCGTC CAGGTAGTGC TTGTGCTGTT CCACAAACAG
            481 CTGTTTCTGC TCATTATCCT CGGGGGAGCC CTTCAGCTTC TCATAGTGGC TGGCCAGGTA
            541 CAGGAAGTTC ACATATTTGG AGGGCAGGGC CAGTTCGTTT CCCTTCTGCA GTTCGCCGGC
            601 AGAGGCCAGC ATTCTCTTCC GGCCGTTTTC CAGCTCGAAC AGGGAGTACT TAGGCAGCTT
            661 GATGATCAGG TCCTTTTTCA CTTCTTTGTA GCCCTTGGCT TCCAGAAAGT CGATGGGATT
            721 CTTCTCGAAG CTGCTTCTTT CCATGATGGT GATCCCCAGC AGCTCTTTCA CACTCTTCAG
            781 TTTCTTGGAC TTGCCCTTTT CCACTTTGGC CACCACCAGC ACAGAATAGG CCACGGTGGG
            841 GGCTGTCGAA AGCCGCCGTA CTTCTTAGGG TCCCAGTCCT TCTTTCTGGC GATCAGCTTT
            901 ATCGCTGTTC CTCTTTGGGC AGGATAGACT CTTTGCTGAA GCCCGCCTGT CTGCACCTCG
            961 GGTCTTTTTT CACGGATATT CACTGGGGCA TGGCTCAGCA CTTTCCGCCA CGATGCCAAA
           1021 TTCCCTGGTC CGTATCCCAC ACGAATCTCC CGGATCGCGA TTGATCTCGA ATCGAGCCGC
          1081 ATG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Chihara, K., Syk-dependent tyrosine phosphorylation of 3BP2 is required for optimal FcRγ-mediated phagocytosis and chemokine expression in U937 cells. Sci. Rep. 7 (1): 11480 (2017). PMID 28904407.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl