RDB15907 - pX330-3BP2 gRNA

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pX330-3BP2 gRNA (#RDB15907)

Targeting construct of human c-Abl SH3 domain-binding protein-2 (3BP2).

Clone info.	Expression vector of gRNA for targeting human c-Abl SH3 domain-binding protein-2 (3BP2) and human codon-optimized SpCas9.
Comment	Effect of the targeting vecctor was confirmed by the depositor with immunoblotting.
Vector backbone	pX330 (plasmid)
Size of vector backbone	8.5 kb
Selectable markers	Amp^r
Gene/insert name	Human SH3BP2 genomic DNA
Depositor\|Developer	Sada, Kiyonao \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Chihara, K et al. Sci Rep. 7(1) : 11480, 2017)

Remarks
The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Chihara, K et al. Sci Rep. 7(1) : 11480, 2017)
Remarks	The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.

必要書類	提供依頼書 [依頼書の記入例 ] 専用MTA（CRISPR/Cas9内包遺伝子材料専用非営利学術目的）をお使いください [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Chihara, K et al. Sci Rep. 7(1) : 11480, 2017)。
備考	本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15907	pX330-3BP2 gRNA	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pX330-3BP2 gRNA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15907).

Reference section:

Chihara, K., Kato, Y., Yoshiki, H., Takeuchi, K., Fujieda, S., Sada, K., Syk-dependent tyrosine phosphorylation of 3BP2 is required for optimal FcRγ-mediated phagocytosis and chemokine expression in U937 cells. Sci. Rep. 7 (1): 11480 (2017). PMID 28904407. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15907_A8Crp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40pA3' element Reverse Sequence file: RDB15907_A8Cra.seq
>D05305A5-1_A8Cr_40pA3'elementRever_E02_14_ABI24.ab1 1 TGTCTAGGGG CGGACTATGG AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT 61 TGCTGGCCTT TTGCTCACAT GTGAGGGCCT ATTTCCCATG ATTCCTTCAT ATTTGCATAT 121 ACGATACAAG GCTGTTAGAG AGATAATTGG AATTAATTTG ACTGTAAACA CAAAGATATT 181 AGTACAAAAT ACGTGACGTA GAAAGTAATA ATTTCTTGGG TAGTTTGCAG TTTTAAAATT 241 ATGTTTTAAA ATGGACTATC ATATGCTTAC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 301 TTTATATATC TTGTGGAAAG GACGAAACAC CGGGTACCGC CCTTCTTGTG CGTTTTAGAG 361 CTAGAAATAG CAAGTTAAAA TAAGGCTAGT CCGTTATCAA CTTGAAAAAG TGGCACCGAG 421 TCGGTGCTTT TTTGTTTTAG AGCTAGAAAT AGCAAGTTAA AATAAGGCTA GTCCGTTTTT 481 AGCGCGTGCG CCAATTCTGC AGACAAATGG CTCTAGAGGT ACCCGTTACA TAACTTACGG 541 TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAGTAACGC 601 CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG 661 CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT 721 GGCCCGCCTG GCATTGTGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 781 TCTACGTATT AGTCATCGCT ATTACCATGG TCGAGGTGAG CCCCACGTTC TGCTTCACTC 841 TCCCCATCTC CCCCCCCCTC CCCACCCCCC AATTTTGTAT TTATTTATTT TTTTAATTAT 901 TTTTGTGCAG CGATGGGGGG CGCGGGGGGG GGGATC //
Primer: RDB13535#1 Sequence file: RDB15907_A8Crb.seq
>D05305A5-1_A8Cr_RDB13535#1_F02_17_ABI24.ab1 1 TGGACATGCC TGAATCACTT TTTTTCAGGT TGGACCGGTG CCACCATGGA CTATAAGGAC 61 CACGACGGAG ACTACAAGGA TCATGATATT GATTACAAAG ACGATGACGA TAAGATGGCC 121 CCAAAGAAGA AGCGGAAGGT CGGTATCCAC GGAGTCCCAG CAGCCGACAA GAAGTACAGC 181 ATCGGCCTGG ACATCGGCAC CAACTCTGTG GGCTGGGCCG TGATCACCGA CGAGTACAAG 241 GTGCCCAGCA AGAAATTCAA GGTGCTGGGC AACACCGACC GGCACAGCAT CAAGAAGAAC 301 CTGATCGGAG CCCTGCTGTT CGACAGCGGC GAAACAGCCG AGGCCACCCG GCTGAAGAGA 361 ACCGCCAGAA GAAGATACAC CAGACGGAAG AACCGGATCT GCTATCTGCA AGAGATCTTC 421 AGCAACGAGA TGGCCAAGGT GGACGACAGC TTCTTCCACA GACTGGAAGA GTCCTTCCTG 481 GTGGAAGAGG ATAAGAAGCA CGAGCGGCAC CCCATCTTCG GCAACATCGT GGACGAGGTG 541 GCCTACCACG AGAAGTACCC CACCATCTAC CACCTGAGAA AGAAACTGGT GGACAGCACC 601 GACAAGGCCG ACCTGCGGCT GATCTATCTG GCCCTGGCCC ACATGATCAA GTTCCGGGGC 661 CACTTCCTGA TCGAGGGCGA CCTGAACCCC GACAACAGCG ACGTGGACAA GCTGTTCATC 721 CAGCTGGTGC AGACCTACAA CCAGCTGTTC GAGGAAAACC CCATCAACGC CAGCGGCGTG 781 GACGCCAAGG CCATCCTGTC TGCCAGACTG AGCAAGAGCA GACGGCTGGA AAATCTGATC 841 GCCCAGCTGC CCGGCGAGAA GAAGAATGGC CTGTTCGGAA ACCTGATTGC CCTGAGCCTG 901 GGCCTGACCC CCCAACTTCA AGAGCAACTT CGACCTGGCC CGAGGGATGC CAAACTGCAG 961 CTGAGCAAAG GACACCCTAC GACGACGACC CTGCACACCC TGCTGGCCCA GATCGGCCGA 1021 CCCAGTACGC CCGAACCTGA TTTCTGGGCC CGCCCAAGAT CTGGTCGGAA CGTCCATTCC 1081 TTGCTGAGCG ACCATTTC //
Primer: BGH_rev Sequence file: RDB15907_A8Crc.seq
>D05305A5-1_A8Cr_BGH_rev2_G02_20_ABI24.ab1 1 CTCTGGGGTA AGGATGCACG GGGGAGGGGC AAACAACAGA TGGCTGGCAA CTAGAAGGCA 61 CAGTCGAGGC TGATCAGCGA GCTCTAGGAA TTCTTACTTT TTCTTTTTTG CCTGGCCGGC 121 CTTTTTCGTG GCCGCCGGCC TTTTGTCGCC TCCCAGCTGA GACAGGTCGA TCCGTGTCTC 181 GTACAGGCCG GTGATGCTCT GGTGGATCAG GGTGGCGTCC AGCACCTCTT TGGTGCTGGT 241 GTACCTCTTC CGGTCGATGG TGGTGTCAAA GTACTTGAAG GCGGCAGGGG CTCCCAGATT 301 GGTCAGGGTA AACAGGTGGA TGATATTCTC GGCCTGCTCT CTGATGGGCT TATCCCGGTG 361 CTTGTTGTAG GCGGACAGCA CTTTGTCCAG ATTAGCGTCG GCCAGGATCA CTCTCTTGGA 421 GAACTCGCTG ATCTGCTCGA TGATCTCGTC CAGGTAGTGC TTGTGCTGTT CCACAAACAG 481 CTGTTTCTGC TCATTATCCT CGGGGGAGCC CTTCAGCTTC TCATAGTGGC TGGCCAGGTA 541 CAGGAAGTTC ACATATTTGG AGGGCAGGGC CAGTTCGTTT CCCTTCTGCA GTTCGCCGGC 601 AGAGGCCAGC ATTCTCTTCC GGCCGTTTTC CAGCTCGAAC AGGGAGTACT TAGGCAGCTT 661 GATGATCAGG TCCTTTTTCA CTTCTTTGTA GCCCTTGGCT TCCAGAAAGT CGATGGGATT 721 CTTCTCGAAG CTGCTTCTTT CCATGATGGT GATCCCCAGC AGCTCTTTCA CACTCTTCAG 781 TTTCTTGGAC TTGCCCTTTT CCACTTTGGC CACCACCAGC ACAGAATAGG CCACGGTGGG 841 GGCTGTCGAA AGCCGCCGTA CTTCTTAGGG TCCCAGTCCT TCTTTCTGGC GATCAGCTTT 901 ATCGCTGTTC CTCTTTGGGC AGGATAGACT CTTTGCTGAA GCCCGCCTGT CTGCACCTCG 961 GGTCTTTTTT CACGGATATT CACTGGGGCA TGGCTCAGCA CTTTCCGCCA CGATGCCAAA 1021 TTCCCTGGTC CGTATCCCAC ACGAATCTCC CGGATCGCGA TTGATCTCGA ATCGAGCCGC 1081 ATG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Chihara, K., Syk-dependent tyrosine phosphorylation of 3BP2 is required for optimal FcRγ-mediated phagocytosis and chemokine expression in U937 cells. Sci. Rep. 7 (1): 11480 (2017). PMID 28904407. [link to RRC of NBRP]

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Other resources in our bank

human SH3BP2

External Database
Human SH3BP2

Reference sequence