Please review the QC test results indicated by icon below as well as clone information before placing your order.

pX330-Abl gRNA

Targeting construct of human c-Abl (Abl).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Chihara, K et al. Sci Rep. 7(1) : 11480, 2017)
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
Remarks	The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Chihara, K et al. Sci Rep. 7(1) : 11480, 2017)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 専用MTA（CRISPR/Cas9内包遺伝子材料専用 非営利学術目的）をお使いください [Word]
備考	本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15906_A8Crp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40pA3' element Reverse Sequence file: RDB15906_A8Cra.seq
>D05305A4-1_A8Cr_40pA3'elementRever_B02_05_ABI24.ab1     1 TTTTCAGGGG GGGCGGGACC TATGGAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG    61 CCTTTTGCTG GCCTTTTGCT CACATGTGAG GGCCTATTTC CCATGATTCC TTCATATTTG   121 CATATACGAT ACAAGGCTGT TAGAGAGATA ATTGGAATTA ATTTGACTGT AAACACAAAG   181 ATATTAGTAC AAAATACGTG ACGTAGAAAG TAATAATTTC TTGGGTAGTT TGCAGTTTTA   241 AAATTATGTT TTAAAATGGA CTATCATATG CTTACCGTAA CTTGAAAGTA TTTCGATTTC   301 TTGGCTTTAT ATATCTTGTG GAAAGGACGA AACACCGTCT GAGTGAAGCC GCTCGTGTTT   361 TAGAGCTAGA AATAGCAAGT TAAAATAAGG CTAGTCCGTT ATCAACTTGA AAAAGTGGCA   421 CCGAGTCGGT GCTTTTTTGT TTTAGAGCTA GAAATAGCAA GTTAAAATAA GGCTAGTCCG   481 TTTTTAGCGC GTGCGCCAAT TCTGCAGACA AATGGCTCTA GAGGTACCCG TTACATAACT   541 TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAGT   601 AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA   661 CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG   721 TAAATGGCCC GCCTGGCATT GTGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA   781 GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTCGAG GTGAGCCCCA CGTTCTGCTT   841 CACTCTCCCC ATCTCCCCCC CCCTCCCCAC CCCCCAATTT TGTATTTATT TATTTTTTTA 901 ATTATTTTTG TGCAGCGATG GGGGGCAGGG GGGGGGGGGG TG //
Primer: RDB13535#1 Sequence file: RDB15906_A8Crb.seq
>D05305A4-1_A8Cr_RDB13535#1_C02_08_ABI24.ab1     1 GTGGAAAGTG GCCTGAATCA CTTTTTTTCA GGTTGGACCG GTGCCACCAT GGACTATAAG    61 GACCACGACG GAGATTACAA GGATCATGAT ATTGATTACA AAGACGATGA CGATAAGATG   121 GCCCCAAAGA AGAAGCGGAA GGTCGGTATC CACGGAGTCC CAGCAGCCGA CAAGAAGTAC   181 AGCATCGGCC TGGACATCGG CACCAACTCT GTGGGCTGGG CCGTGATCAC CGACGAGTAC   241 AAGGTGCCCA GCAAGAAATT CAAGGTGCTG GGCAACACCG ACCGGCACAG CATCAAGAAG   301 AACCTGATCG GAGCCCTGCT GTTCGACAGC GGCGAAACAG CCGAGGCCAC CCGGCTGAAG   361 AGAACCGCCA GAAGAAGATA CACCAGACGG AAGAACCGGA TCTGCTATCT GCAAGAGATC   421 TTCAGCAACG AGATGGCCAA GGTGGACGAC AGCTTCTTCC ACAGACTGGA AGAGTCCTTC   481 CTGGTGGAAG AGGATAAGAA GCACGAGCGG CACCCCATCT TCGGCAACAT CGTGGACGAG   541 GTGGCCTACC ACGAGAAGTA CCCCACCATC TACCACCTGA GAAAGAAACT GGTGGACAGC   601 ACCGACAAGG CCGACCTGCG GCTGATCTAT CTGGCCCTGG CCCACATGAT CAAGTTCCGG   661 GGCCACTTCC TGATCGAGGG CGACCTGAAC CCCGACAACA GCGACGTGGA CAAGCTGTTC   721 ATCCAGCTGG TGCAGACCTA CAACCAGCTG TTCGAGGAAA ACCCCATCAA CGCCAGCGGC   781 GTGGACGCCA AGGCCATCCT GTCTGCCAGA CTGAGCAAGA GCAGACGGCT GGAAAATCTG   841 ATCGCCCAGC TGCCCGGCGA GAAGAAGAAT GGCCTGTTCG GAAACCTGAT TGCCCTGAGC   901 CTGGGCCTGA CCCCCCAACT TCAAGAGCAA CTTCGACCTG CCCGAGGATG CCAAACTGCA   961 GCTGAGCAAA GGACACCTAC GACGACGACC CTGACAACCC TGCTGGGCCC AGAATCGGCG  1021 GACCCAGTTA CGCCGACCCT GGTTTCTGTG GCTAGGAAAC CTGTCGACGT CATTCTTGCC 1081 TGAAGCGACA TCCTGGAGAA AGT //
Primer: BGH_rev Sequence file: RDB15906_A8Crc.seq
>D05305A4-1_A8Cr_BGH_rev2_D02_11_ABI24.ab1     1 CAACGGGGTC AGAAGCACGG GGGAGGGGCA AACAACAGAT GGCTGGCAAC TAGAAGGCAC    61 AGTCGAGGCT GATCAGCGAG CTCTAGGAAT TCTTACTTTT TCTTTTTTGC CTGGCCGGCC   121 TTTTTCGTGG CCGCCGGCCT TTTGTCGCCT CCCAGCTGAG ACAGGTCGAT CCGTGTCTCG   181 TACAGGCCGG TGATGCTCTG GTGGATCAGG GTGGCGTCCA GCACCTCTTT GGTGCTGGTG   241 TACCTCTTCC GGTCGATGGT GGTGTCAAAG TACTTGAAGG CGGCAGGGGC TCCCAGATTG   301 GTCAGGGTAA ACAGGTGGAT GATATTCTCG GCCTGCTCTC TGATGGGCTT ATCCCGGTGC   361 TTGTTGTAGG CGGACAGCAC TTTGTCCAGA TTAGCGTCGG CCAGGATCAC TCTCTTGGAG   421 AACTCGCTGA TCTGCTCGAT GATCTCGTCC AGGTAGTGCT TGTGCTGTTC CACAAACAGC   481 TGTTTCTGCT CATTATCCTC GGGGGAGCCC TTCAGCTTCT CATAGTGGCT GGCCAGGTAC   541 AGGAAGTTCA CATATTTGGA GGGCAGGGCC AGTTCGTTTC CCTTCTGCAG TTCGCCGGCA   601 GAGGCCAGCA TTCTCTTCCG GCCGTTTTCC AGCTCGAACA GGGAGTACTT AGGCAGCTTG   661 ATGATCAGGT CCTTTTTCAC TTCTTTGTAG CCCTTGGCTT CCAGAAAGTC GATGGGATTC   721 TTCTCGAAGC TGCTTCTTTC CATGATGGTG ATCCCCAGCA GCTCTTTCAC ACTCTTCAGT   781 TTCTTGGACT TGCCCTTTTC CACTTTGGCC ACCACCAGCA CAGAATAGGC CACGGTGGGG   841 GCTGTCGAAG CCGCCGTACT TCTTAGGGTC CCAGTCCTTC TTTCTGGCGA TCAGCTTATC   901 GCTGTTCCTC TTGGGCAGGA TAGACTCTTT GCTGAAGCCG CCTGTCTGCA CCTCGGTCTT   961 TTTTCACGAT ATTCACTTGG GGGCATGCTC AGCACTTTTC CCGCACGGGT GGCAAAAATT  1021 CCCCGGCCCC TTATCCCACA CGATCTCCCC GGCTTCGACG GATTGCTCTC GGATCAGAAA 1081 GGTCCGGCT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl