RDB15906 - pX330-Abl gRNA

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pX330-Abl gRNA (#RDB15906)

Targeting construct of human c-Abl (Abl).

Clone info.	Expression vector of gRNA for targeting human c-Abl (Abl) and human codon-optimized SpCas9.
Comment	Effect of the targeting vecctor was confirmed by the depositor with immunoblotting.
Vector backbone	pX330 (plasmid)
Size of vector backbone	8.5 kb
Selectable markers	Amp^r
Gene/insert name	Human ABL genomic DNA
Depositor\|Developer	Sada, Kiyonao \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Chihara, K et al. Sci Rep. 7(1) : 11480, 2017)

Remarks
The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Chihara, K et al. Sci Rep. 7(1) : 11480, 2017)
Remarks	The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.

必要書類	提供依頼書 [依頼書の記入例 ] 専用MTA（CRISPR/Cas9内包遺伝子材料専用非営利学術目的）をお使いください [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Chihara, K et al. Sci Rep. 7(1) : 11480, 2017)。
備考	本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15906	pX330-Abl gRNA	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pX330-Abl gRNA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15906).

Reference section:

Chihara, K., Kato, Y., Yoshiki, H., Takeuchi, K., Fujieda, S., Sada, K., Syk-dependent tyrosine phosphorylation of 3BP2 is required for optimal FcRγ-mediated phagocytosis and chemokine expression in U937 cells. Sci. Rep. 7 (1): 11480 (2017). PMID 28904407. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15906_A8Crp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40pA3' element Reverse Sequence file: RDB15906_A8Cra.seq
>D05305A4-1_A8Cr_40pA3'elementRever_B02_05_ABI24.ab1 1 TTTTCAGGGG GGGCGGGACC TATGGAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG 61 CCTTTTGCTG GCCTTTTGCT CACATGTGAG GGCCTATTTC CCATGATTCC TTCATATTTG 121 CATATACGAT ACAAGGCTGT TAGAGAGATA ATTGGAATTA ATTTGACTGT AAACACAAAG 181 ATATTAGTAC AAAATACGTG ACGTAGAAAG TAATAATTTC TTGGGTAGTT TGCAGTTTTA 241 AAATTATGTT TTAAAATGGA CTATCATATG CTTACCGTAA CTTGAAAGTA TTTCGATTTC 301 TTGGCTTTAT ATATCTTGTG GAAAGGACGA AACACCGTCT GAGTGAAGCC GCTCGTGTTT 361 TAGAGCTAGA AATAGCAAGT TAAAATAAGG CTAGTCCGTT ATCAACTTGA AAAAGTGGCA 421 CCGAGTCGGT GCTTTTTTGT TTTAGAGCTA GAAATAGCAA GTTAAAATAA GGCTAGTCCG 481 TTTTTAGCGC GTGCGCCAAT TCTGCAGACA AATGGCTCTA GAGGTACCCG TTACATAACT 541 TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAGT 601 AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA 661 CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG 721 TAAATGGCCC GCCTGGCATT GTGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA 781 GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTCGAG GTGAGCCCCA CGTTCTGCTT 841 CACTCTCCCC ATCTCCCCCC CCCTCCCCAC CCCCCAATTT TGTATTTATT TATTTTTTTA 901 ATTATTTTTG TGCAGCGATG GGGGGCAGGG GGGGGGGGGG TG //
Primer: RDB13535#1 Sequence file: RDB15906_A8Crb.seq
>D05305A4-1_A8Cr_RDB13535#1_C02_08_ABI24.ab1 1 GTGGAAAGTG GCCTGAATCA CTTTTTTTCA GGTTGGACCG GTGCCACCAT GGACTATAAG 61 GACCACGACG GAGATTACAA GGATCATGAT ATTGATTACA AAGACGATGA CGATAAGATG 121 GCCCCAAAGA AGAAGCGGAA GGTCGGTATC CACGGAGTCC CAGCAGCCGA CAAGAAGTAC 181 AGCATCGGCC TGGACATCGG CACCAACTCT GTGGGCTGGG CCGTGATCAC CGACGAGTAC 241 AAGGTGCCCA GCAAGAAATT CAAGGTGCTG GGCAACACCG ACCGGCACAG CATCAAGAAG 301 AACCTGATCG GAGCCCTGCT GTTCGACAGC GGCGAAACAG CCGAGGCCAC CCGGCTGAAG 361 AGAACCGCCA GAAGAAGATA CACCAGACGG AAGAACCGGA TCTGCTATCT GCAAGAGATC 421 TTCAGCAACG AGATGGCCAA GGTGGACGAC AGCTTCTTCC ACAGACTGGA AGAGTCCTTC 481 CTGGTGGAAG AGGATAAGAA GCACGAGCGG CACCCCATCT TCGGCAACAT CGTGGACGAG 541 GTGGCCTACC ACGAGAAGTA CCCCACCATC TACCACCTGA GAAAGAAACT GGTGGACAGC 601 ACCGACAAGG CCGACCTGCG GCTGATCTAT CTGGCCCTGG CCCACATGAT CAAGTTCCGG 661 GGCCACTTCC TGATCGAGGG CGACCTGAAC CCCGACAACA GCGACGTGGA CAAGCTGTTC 721 ATCCAGCTGG TGCAGACCTA CAACCAGCTG TTCGAGGAAA ACCCCATCAA CGCCAGCGGC 781 GTGGACGCCA AGGCCATCCT GTCTGCCAGA CTGAGCAAGA GCAGACGGCT GGAAAATCTG 841 ATCGCCCAGC TGCCCGGCGA GAAGAAGAAT GGCCTGTTCG GAAACCTGAT TGCCCTGAGC 901 CTGGGCCTGA CCCCCCAACT TCAAGAGCAA CTTCGACCTG CCCGAGGATG CCAAACTGCA 961 GCTGAGCAAA GGACACCTAC GACGACGACC CTGACAACCC TGCTGGGCCC AGAATCGGCG 1021 GACCCAGTTA CGCCGACCCT GGTTTCTGTG GCTAGGAAAC CTGTCGACGT CATTCTTGCC 1081 TGAAGCGACA TCCTGGAGAA AGT //
Primer: BGH_rev Sequence file: RDB15906_A8Crc.seq
>D05305A4-1_A8Cr_BGH_rev2_D02_11_ABI24.ab1 1 CAACGGGGTC AGAAGCACGG GGGAGGGGCA AACAACAGAT GGCTGGCAAC TAGAAGGCAC 61 AGTCGAGGCT GATCAGCGAG CTCTAGGAAT TCTTACTTTT TCTTTTTTGC CTGGCCGGCC 121 TTTTTCGTGG CCGCCGGCCT TTTGTCGCCT CCCAGCTGAG ACAGGTCGAT CCGTGTCTCG 181 TACAGGCCGG TGATGCTCTG GTGGATCAGG GTGGCGTCCA GCACCTCTTT GGTGCTGGTG 241 TACCTCTTCC GGTCGATGGT GGTGTCAAAG TACTTGAAGG CGGCAGGGGC TCCCAGATTG 301 GTCAGGGTAA ACAGGTGGAT GATATTCTCG GCCTGCTCTC TGATGGGCTT ATCCCGGTGC 361 TTGTTGTAGG CGGACAGCAC TTTGTCCAGA TTAGCGTCGG CCAGGATCAC TCTCTTGGAG 421 AACTCGCTGA TCTGCTCGAT GATCTCGTCC AGGTAGTGCT TGTGCTGTTC CACAAACAGC 481 TGTTTCTGCT CATTATCCTC GGGGGAGCCC TTCAGCTTCT CATAGTGGCT GGCCAGGTAC 541 AGGAAGTTCA CATATTTGGA GGGCAGGGCC AGTTCGTTTC CCTTCTGCAG TTCGCCGGCA 601 GAGGCCAGCA TTCTCTTCCG GCCGTTTTCC AGCTCGAACA GGGAGTACTT AGGCAGCTTG 661 ATGATCAGGT CCTTTTTCAC TTCTTTGTAG CCCTTGGCTT CCAGAAAGTC GATGGGATTC 721 TTCTCGAAGC TGCTTCTTTC CATGATGGTG ATCCCCAGCA GCTCTTTCAC ACTCTTCAGT 781 TTCTTGGACT TGCCCTTTTC CACTTTGGCC ACCACCAGCA CAGAATAGGC CACGGTGGGG 841 GCTGTCGAAG CCGCCGTACT TCTTAGGGTC CCAGTCCTTC TTTCTGGCGA TCAGCTTATC 901 GCTGTTCCTC TTGGGCAGGA TAGACTCTTT GCTGAAGCCG CCTGTCTGCA CCTCGGTCTT 961 TTTTCACGAT ATTCACTTGG GGGCATGCTC AGCACTTTTC CCGCACGGGT GGCAAAAATT 1021 CCCCGGCCCC TTATCCCACA CGATCTCCCC GGCTTCGACG GATTGCTCTC GGATCAGAAA 1081 GGTCCGGCT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Chihara, K., Syk-dependent tyrosine phosphorylation of 3BP2 is required for optimal FcRγ-mediated phagocytosis and chemokine expression in U937 cells. Sci. Rep. 7 (1): 11480 (2017). PMID 28904407. [link to RRC of NBRP]

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human ABL

External Database
Human ABL

Reference sequence