Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pX330-Hck gRNA (#RDB15905)

Targeting construct of human hematopoietic cell kinase (Hck).

Clone info. Expression vector of gRNA for targeting human hematopoietic cell kinase (Hck) and human codon-optimized SpCas9.
Comment Effect of the targeting vecctor was confirmed by the depositor with immunoblotting.
Vector backbone pX330 (plasmid)
Size of vector backbone 8.5 kb
Selectable markers Amp^r
Gene/insert name Human HCK genomic DNA
Depositor|Developer Sada, Kiyonao |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Chihara, K et al. Sci Rep. 7(1) : 11480, 2017)
Remarks
The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
専用MTA（CRISPR/Cas9内包遺伝子材料専用 非営利学術目的）をお使いください [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Chihara, K et al. Sci Rep. 7(1) : 11480, 2017)。
備考
本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15905 pX330-Hck gRNA DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pX330-Hck gRNA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15905).

Reference section:

Chihara, K., Kato, Y., Yoshiki, H., Takeuchi, K., Fujieda, S., Sada, K., Syk-dependent tyrosine phosphorylation of 3BP2 is required for optimal FcRγ-mediated phagocytosis and chemokine expression in U937 cells. Sci. Rep. 7 (1): 11480 (2017). PMID 28904407. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15905_A8Crp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40pA3' element Reverse
Sequence file: RDB15905_A8Cra.seq check
>D05305A3-2_A8Cr_40pA3'elementRever_G01_19_ABI24.ab1
    1 GACAGGAGGC GGAGCCTATG GAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT
   61 TTTGCTGGCC TTTTGCTCAC ATGTGAGGGC CTATTTCCCA TGATTCCTTC ATATTTGCAT
  121 ATACGATACA AGGCTGTTAG AGAGATAATT GGAATTAATT TGACTGTAAA CACAAAGATA
  181 TTAGTACAAA ATACGTGACG TAGAAAGTAA TAATTTCTTG GGTAGTTTGC AGTTTTAAAA
  241 TTATGTTTTA AAATGGACTA TCATATGCTT ACCGTAACTT GAAAGTATTT CGATTTCTTG
  301 GCTTTATATA TCTTGTGGAA AGGACGAAAC ACCGTGGGAT CCGGCACGTA CACGTTTTAG
  361 AGCTAGAAAT AGCAAGTTAA AATAAGGCTA GTCCGTTATC AACTTGAAAA AGTGGCACCG
  421 AGTCGGTGCT TTTTTGTTTT AGAGCTAGAA ATAGCAAGTT AAAATAAGGC TAGTCCGTTT
  481 TTAGCGCGTG CGCCAATTCT GCAGACAAAT GGCTCTAGAG GTACCCGTTA CATAACTTAC
  541 GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAGTAAC
  601 GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT
  661 GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA
  721 ATGGCCCGCC TGGCATTGTG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA
  781 CATCTACGTA TTAGTCATCG CTATTACCAT GGTCGAGGTG AGCCCCACGT TCTGCTTCAC
  841 TCTCCCCATC TCCCCCCCCC CTCCCCACCC CCCAATTTTG TATTTATTTT ATTTTTTTTA
901 ATTTATTTTT GTGCAGCGAT GGGGGGCGCG GGGGGGGGG
//
Primer: RDB13535#1
Sequence file: RDB15905_A8Crb.seq check
>D05305A3-2_A8Cr_RDB13535#1_H01_22_ABI24.ab1
    1 TTGAACATGA CCTGAATCAC TTTTTTTCAG GTTGGACCGG TGCCACCATG GACTATAAGG
   61 ACCACGACGG AGACTACAAG GATCATGATA TTGATTACAA AGACGATGAC GATAAGATGG
  121 CCCCAAAGAA GAAGCGGAAG GTCGGTATCC ACGGAGTCCC AGCAGCCGAC AAGAAGTACA
  181 GCATCGGCCT GGACATCGGC ACCAACTCTG TGGGCTGGGC CGTGATCACC GACGAGTACA
  241 AGGTGCCCAG CAAGAAATTC AAGGTGCTGG GCAACACCGA CCGGCACAGC ATCAAGAAGA
  301 ACCTGATCGG AGCCCTGCTG TTCGACAGCG GCGAAACAGC CGAGGCCACC CGGCTGAAGA
  361 GAACCGCCAG AAGAAGATAC ACCAGACGGA AGAACCGGAT CTGCTATCTG CAAGAGATCT
  421 TCAGCAACGA GATGGCCAAG GTGGACGACA GCTTCTTCCA CAGACTGGAA GAGTCCTTCC
  481 TGGTGGAAGA GGATAAGAAG CACGAGCGGC ACCCCATCTT CGGCAACATC GTGGACGAGG
  541 TGGCCTACCA CGAGAAGTAC CCCACCATCT ACCACCTGAG AAAGAAACTG GTGGACAGCA
  601 CCGACAAGGC CGACCTGCGG CTGATCTATC TGGCCCTGGC CCACATGATC AAGTTCCGGG
  661 GCCACTTCCT GATCGAGGGC GACCTGAACC CCGACAACAG CGACGTGGAC AAGCTGTTCA
  721 TCCAGCTGGT GCAGACCTAC AACCAGCTGT TCGAGGAAAA CCCCATCAAC GCCAGCGGCG
  781 TGGACGCCAA GGCCATCCTG TCTGCCAGAC TGAGCAAGAG CAGACGGCTG GGAAAATCTG
  841 ATCGCCCAGC TGCCCGGCGA GAAGAAGAAT GGCCTGTTTC GGAAACCTGA TTGCCCTGAG
  901 CCCTGGGCCT GACCCCCAAC TTTCAAGAGC AACTTTCGAC CTGTCGGAGG ATGGCCAAAC
  961 TGCAGCTGAG CAGGACACCT ACCGAACGAC CGACTGACAC TGCTTGGTCC AGATCCGCGA
 1021 CAGTACGCGA ACTGGTTTCT GCGCAGGACT GGTCGGACGC CATCTGGCTG ACGACATTCC
1081 GTG
//
Primer: BGH_rev
Sequence file: RDB15905_A8Crc.seq check
>D05305A3-2_A8Cr_BGH_rev2_A02_02_ABI24.ab1
    1 GTTGGGGGCC AGGAGGCACG GGGGAGGGGC AAACAACAGA TGGCTGGCAA CTAGAAGGCA
   61 CAGTCGAGGC TGATCAGCGA GCTCTAGGAA TTCTTACTTT TTCTTTTTTG CCTGGCCGGC
  121 CTTTTTCGTG GCCGCCGGCC TTTTGTCGCC TCCCAGCTGA GACAGGTCGA TCCGTGTCTC
  181 GTACAGGCCG GTGATGCTCT GGTGGATCAG GGTGGCGTCC AGCACCTCTT TGGTGCTGGT
  241 GTACCTCTTC CGGTCGATGG TGGTGTCAAA GTACTTGAAG GCGGCAGGGG CTCCCAGATT
  301 GGTCAGGGTA AACAGGTGGA TGATATTCTC GGCCTGCTCT CTGATGGGCT TATCCCGGTG
  361 CTTGTTGTAG GCGGACAGCA CTTTGTCCAG ATTAGCGTCG GCCAGGATCA CTCTCTTGGA
  421 GAACTCGCTG ATCTGCTCGA TGATCTCGTC CAGGTAGTGC TTGTGCTGTT CCACAAACAG
  481 CTGTTTCTGC TCATTATCCT CGGGGGAGCC CTTCAGCTTC TCATAGTGGC TGGCCAGGTA
  541 CAGGAAGTTC ACATATTTGG AGGGCAGGGC CAGTTCGTTT CCCTTCTGCA GTTCGCCGGC
  601 AGAGGCCAGC ATTCTCTTCC GGCCGTTTTC CAGCTCGAAC AGGGAGTACT TAGGCAGCTT
  661 GATGATCAGG TCCTTTTTCA CTTCTTTGTA GCCCTTGGCT TCCAGAAAGT CGATGGGATT
  721 CTTCTCGAAG CTGCTTCTTT CCATGATGGT GATCCCCAGC AGCTCTTTCA CACTCTTCAG
  781 TTTCTTGGAC TTGCCCTTTT CCACTTTGGC CACCACCAGC ACAGAATAGG CCACGGGTGG
  841 GGCTGTCGAA GCCGCCGTAC TTCTTAGGGT CCCAGTCCTT CTTTCTGGCG ATCAGCTTAT
  901 CGCTGTTTCC TCTTGGGCAG GATAGACTCT TTGCTGAAGC CCGCCTGTCT GCACCTCGGT
  961 CTTTTTTCAC GATATTCACT TTGGGGCATG CTCAGCACTT TCCGGCACGG TGGCCAAAAT
 1021 CCCCGGCCCT TAATCCACAC GAATCTCCCC CGAATTCGTC GATTGTCTCG ATCAGAGGTC
1081 GCATCCTGGA ATTC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Chihara, K., Syk-dependent tyrosine phosphorylation of 3BP2 is required for optimal FcRγ-mediated phagocytosis and chemokine expression in U937 cells. Sci. Rep. 7 (1): 11480 (2017). PMID 28904407. [link to RRC of NBRP]