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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pX330-Syk gRNA

Targeting construct of human spleen tyrosine kinase (Syk).

Catalog number RDB15904
Resource name pX330-Syk gRNA
Clone info. Expression vector of gRNA for targeting human spleen tyrosine kinase (Syk) and human codon-optimized SpCas9.
Effect of the targeting vecctor was confirmed by the depositor with immunoblotting.
Vector backbone pX330 (plasmid)
Size of vector backbone 8.5 kb
Selectable markers Amp^r
Gene/insert name Human SYK genomic DNA
Depositor|Developer Sada, Kiyonao |
 
Other clones in our bank human SYK (NCBI Gene 6850) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Chihara, K et al. Sci Rep. 7(1) : 11480, 2017)
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Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
Remarks
The BIOLOGICAL RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. Please refer the Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose).
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15904 pX330-Syk gRNA DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Chihara, K., Syk-dependent tyrosine phosphorylation of 3BP2 is required for optimal FcRγ-mediated phagocytosis and chemokine expression in U937 cells. Sci. Rep. 7 (1): 11480 (2017). PMID 28904407.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15904_A8Crp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40pA3' element Reverse
Sequence file: RDB15904_A8Cra.seq check
>D05305A2-1_A8Cr_40pA3'elementRever_D01_10_ABI24.ab1
    1 TTTTAGGTGG GCGGACCTAT GGAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT
   61 TTTGCTGGCC TTTTGCTCAC ATGTGAGGGC CTATTTCCCA TGATTCCTTC ATATTTGCAT
  121 ATACGATACA AGGCTGTTAG AGAGATAATT GGAATTAATT TGACTGTAAA CACAAAGATA
  181 TTAGTACAAA ATACGTGACG TAGAAAGTAA TAATTTCTTG GGTAGTTTGC AGTTTTAAAA
  241 TTATGTTTTA AAATGGACTA TCATATGCTT ACCGTAACTT GAAAGTATTT CGATTTCTTG
  301 GCTTTATATA TCTTGTGGAA AGGACGAAAC ACCGTTTCGG CAACATCACC CGGGGTTTTA
  361 GAGCTAGAAA TAGCAAGTTA AAATAAGGCT AGTCCGTTAT CAACTTGAAA AAGTGGCACC
  421 GAGTCGGTGC TTTTTTGTTT TAGAGCTAGA AATAGCAAGT TAAAATAAGG CTAGTCCGTT
  481 TTTAGCGCGT GCGCCAATTC TGCAGACAAA TGGCTCTAGA GGTACCCGTT ACATAACTTA
  541 CGGTAAATGG CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAGTAA
  601 CGCCAATAGG GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT
  661 TGGCAGTACA TCAAGTGTAT CATATGCCAA GTACGCCCCC TATTGACGTC AATGACGGTA
  721 AATGGCCCGC CTGGCATTGT GCCCAGTACA TGACCTTATG GGACTTTCCT ACTTGGCAGT
  781 ACATCTACGT ATTAGTCATC GCTATTACCA TGGTCGAGGT GAGCCCCCAC GTTCTGCTTC
  841 ACTCTCCCCA TCTCCCCCCC CCCTCCCCAC CCCCCAATTT TTGTATTTTA TTTTATTTTT
901 TTTAATTTAT TTTTGTGCAG CCGATGGGGG GCGGGGGGGG GGGGGGGACG
//
Primer: RDB13535#1
Sequence file: RDB15904_A8Crb.seq check
>D05305A2-1_A8Cr_RDB13535#1_E01_13_ABI24.ab1
    1 TGACAAGGGC CTGAATCACT TTTTTTCAGG TTGGACCGGT GCCACCATGG ACTATAAGGA
   61 CCACGACGGA GCACTACAAG GATCATGATA TTGATTACAA AGACGATGAC GATAAGATGG
  121 CCCCAAAGAA GAAGCGGAAG GTCGGTATCC ACGGAGTCCC AGCAGCCGAC AAGAAGTACA
  181 GCATCGGCCT GGACATCGGC ACCAACTCTG TGGGCTGGGC CGTGATCACC GACGAGTACA
  241 AGGTGCCCAG CAAGAAATTC AAGGTGCTGG GCAACACCGA CCGGCACAGC ATCAAGAAGA
  301 ACCTGATCGG AGCCCTGCTG TTCGACAGCG GCGAAACAGC CGAGGCCACC CGGCTGAAGA
  361 GAACCGCCAG AAGAAGATAC ACCAGACGGA AGAACCGGAT CTGCTATCTG CAAGAGATCT
  421 TCAGCAACGA GATGGCCAAG GTGGACGACA GCTTCTTCCA CAGACTGGAA GAGTCCTTCC
  481 TGGTGGAAGA GGATAAGAAG CACGAGCGGC ACCCCATCTT CGGCAACATC GTGGACGAGG
  541 TGGCCTACCA CGAGAAGTAC CCCACCATCT ACCACCTGAG AAAGAAACTG GTGGACAGCA
  601 CCGACAGGCC GACCTGCGGC TGATCTATCT GGCCCTGGCC CACATGATCA AGTTCCGGGG
  661 CCACTTCCTG ATCGAGGGCG ACCTGAACCC CGACAACAGC GACGTGGACA AGCTGTTCAT
  721 CCAGCTGGTG CAGACCTACA ACCAGCTGTT CGAGGAAAAC CCCATCAACG CCAGCGGCGT
  781 GGACGCCCAA GGCCATCCTG TCTGCCAGAC TGAGCAAGAG CAGACGGCTG GGAAAATCTG
  841 ATCGGCCCAG CTGCCCGCCG AGAAGAAGAA TGGCCCTGTT CGGAAACCTG ATTGCCTGAG
  901 CCTGGGCCTG GACCCCCAAC TTCAGAACAC TTCGACCTGG TCGAGGATGC CAACCTGCAG
  961 CTGAGCAAGA CACCCTTACG AACGAACGAC TGGAACACCT GGCTGGCCGA ATCCGCGACA
1021 GATACGCGAA CTGTTCTGGC CGCAGACTGT CGAGTCATCC TGGCTGAACG G
//
Primer: BGH_rev
Sequence file: RDB15904_A8Crc.seq check
>D05305A2-1_A8Cr_BGH_rev2_F01_16_ABI24.ab1
    1 TATGGGTACA GCACGGGGGA GGGGCAAACA ACAGATGGCT GGCAACTAGA AGGCACAGTC
   61 GAGGCTGATC AGCGAGCTCT AGGAATTCTT ACTTTTTCTT TTTTGCCTGG CCGGCCTTTT
  121 TCGTGGCCGC CGGCCTTTTG TCGCCTCCCA GCTGAGACAG GTCGATCCGT GTCTCGTACA
  181 GGCCGGTGAT GCTCTGGTGG ATCAGGGTGG CGTCCAGCAC CTCTTTGGTG CTGGTGTACC
  241 TCTTCCGGTC GATGGTGGTG TCAAAGTACT TGAAGGCGGC AGGGGCTCCC AGATTGGTCA
  301 GGGTAAACAG GTGGATGATA TTCTCGGCCT GCTCTCTGAT GGGCTTATCC CGGTGCTTGT
  361 TGTAGGCGGA CAGCACTTTG TCCAGATTAG CGTCGGCCAG GATCACTCTC TTGGAGAACT
  421 CGCTGATCTG CTCGATGATC TCGTCCAGGT AGTGCTTGTG CTGTTCCACA AACAGCTGTT
  481 TCTGCTCATT ATCCTCGGGG GAGCCCTTCA GCTTCTCATA GTGGCTGGCC AGGTACAGGA
  541 AGTTCACATA TTTGGAGGGC AGGGCCAGTT CGTTTCCCTT CTGCAGTTCG CCGGCAGAGG
  601 CCAGCATTCT CTTCCGGCCG TTTTCCAGCT CGAACAGGGA GTACTTAGGC AGCTTGATGA
  661 TCAGGTCCTT TTTCACTTCT TTGTAGCCCT TGGCTTCCAG AAAGTCGATG GGATTCTTCT
  721 CGAAGCTGCT TCTTTCCATG ATGGTGATCC CCAGCAGCTC TTTCACACTC TTCAGTTTCT
  781 TGGACTTGCC CTTTTCCACT TTGGCCACCA CCAGCACAGA ATAGGCCACG GTGGGGCTGT
  841 CGAAGCCGCC GTACTTCTTA GGGTCCCAGT CCTTCTTTCT GGCGATCAGC TTATCGCTGT
  901 TCCTCTTGGG CAGGATAGAC TCTTTGCTGA AGCCGCCCTG TCTGCACCCT CGGTCTTTTT
  961 TCACGATATT CACTTTGGGG GCATGCTCAG CACTTTCCGC CACGGTGCAA AATCCCGGCT
1021 GATATCCCAC ACGATCTCCC TGAATTCGAC CGATTTCTCG AATCAGAGGA CGCCT
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Chihara, K., Syk-dependent tyrosine phosphorylation of 3BP2 is required for optimal FcRγ-mediated phagocytosis and chemokine expression in U937 cells. Sci. Rep. 7 (1): 11480 (2017). PMID 28904407.

2021.03.11

GNP_filter3_RDBDEP_html_210308.pl