Please review the QC test results indicated by icon below as well as clone information before placing your order.

pX330-Syk gRNA

Targeting construct of human spleen tyrosine kinase (Syk).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Chihara, K et al. Sci Rep. 7(1) : 11480, 2017)
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
Remarks	The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Chihara, K et al. Sci Rep. 7(1) : 11480, 2017)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 専用MTA（CRISPR/Cas9内包遺伝子材料専用 非営利学術目的）をお使いください [Word]
備考	本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15904_A8Crp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40pA3' element Reverse Sequence file: RDB15904_A8Cra.seq
>D05305A2-1_A8Cr_40pA3'elementRever_D01_10_ABI24.ab1     1 TTTTAGGTGG GCGGACCTAT GGAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT    61 TTTGCTGGCC TTTTGCTCAC ATGTGAGGGC CTATTTCCCA TGATTCCTTC ATATTTGCAT   121 ATACGATACA AGGCTGTTAG AGAGATAATT GGAATTAATT TGACTGTAAA CACAAAGATA   181 TTAGTACAAA ATACGTGACG TAGAAAGTAA TAATTTCTTG GGTAGTTTGC AGTTTTAAAA   241 TTATGTTTTA AAATGGACTA TCATATGCTT ACCGTAACTT GAAAGTATTT CGATTTCTTG   301 GCTTTATATA TCTTGTGGAA AGGACGAAAC ACCGTTTCGG CAACATCACC CGGGGTTTTA   361 GAGCTAGAAA TAGCAAGTTA AAATAAGGCT AGTCCGTTAT CAACTTGAAA AAGTGGCACC   421 GAGTCGGTGC TTTTTTGTTT TAGAGCTAGA AATAGCAAGT TAAAATAAGG CTAGTCCGTT   481 TTTAGCGCGT GCGCCAATTC TGCAGACAAA TGGCTCTAGA GGTACCCGTT ACATAACTTA   541 CGGTAAATGG CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAGTAA   601 CGCCAATAGG GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT   661 TGGCAGTACA TCAAGTGTAT CATATGCCAA GTACGCCCCC TATTGACGTC AATGACGGTA   721 AATGGCCCGC CTGGCATTGT GCCCAGTACA TGACCTTATG GGACTTTCCT ACTTGGCAGT   781 ACATCTACGT ATTAGTCATC GCTATTACCA TGGTCGAGGT GAGCCCCCAC GTTCTGCTTC   841 ACTCTCCCCA TCTCCCCCCC CCCTCCCCAC CCCCCAATTT TTGTATTTTA TTTTATTTTT 901 TTTAATTTAT TTTTGTGCAG CCGATGGGGG GCGGGGGGGG GGGGGGGACG //
Primer: RDB13535#1 Sequence file: RDB15904_A8Crb.seq
>D05305A2-1_A8Cr_RDB13535#1_E01_13_ABI24.ab1     1 TGACAAGGGC CTGAATCACT TTTTTTCAGG TTGGACCGGT GCCACCATGG ACTATAAGGA    61 CCACGACGGA GCACTACAAG GATCATGATA TTGATTACAA AGACGATGAC GATAAGATGG   121 CCCCAAAGAA GAAGCGGAAG GTCGGTATCC ACGGAGTCCC AGCAGCCGAC AAGAAGTACA   181 GCATCGGCCT GGACATCGGC ACCAACTCTG TGGGCTGGGC CGTGATCACC GACGAGTACA   241 AGGTGCCCAG CAAGAAATTC AAGGTGCTGG GCAACACCGA CCGGCACAGC ATCAAGAAGA   301 ACCTGATCGG AGCCCTGCTG TTCGACAGCG GCGAAACAGC CGAGGCCACC CGGCTGAAGA   361 GAACCGCCAG AAGAAGATAC ACCAGACGGA AGAACCGGAT CTGCTATCTG CAAGAGATCT   421 TCAGCAACGA GATGGCCAAG GTGGACGACA GCTTCTTCCA CAGACTGGAA GAGTCCTTCC   481 TGGTGGAAGA GGATAAGAAG CACGAGCGGC ACCCCATCTT CGGCAACATC GTGGACGAGG   541 TGGCCTACCA CGAGAAGTAC CCCACCATCT ACCACCTGAG AAAGAAACTG GTGGACAGCA   601 CCGACAGGCC GACCTGCGGC TGATCTATCT GGCCCTGGCC CACATGATCA AGTTCCGGGG   661 CCACTTCCTG ATCGAGGGCG ACCTGAACCC CGACAACAGC GACGTGGACA AGCTGTTCAT   721 CCAGCTGGTG CAGACCTACA ACCAGCTGTT CGAGGAAAAC CCCATCAACG CCAGCGGCGT   781 GGACGCCCAA GGCCATCCTG TCTGCCAGAC TGAGCAAGAG CAGACGGCTG GGAAAATCTG   841 ATCGGCCCAG CTGCCCGCCG AGAAGAAGAA TGGCCCTGTT CGGAAACCTG ATTGCCTGAG   901 CCTGGGCCTG GACCCCCAAC TTCAGAACAC TTCGACCTGG TCGAGGATGC CAACCTGCAG   961 CTGAGCAAGA CACCCTTACG AACGAACGAC TGGAACACCT GGCTGGCCGA ATCCGCGACA 1021 GATACGCGAA CTGTTCTGGC CGCAGACTGT CGAGTCATCC TGGCTGAACG G //
Primer: BGH_rev Sequence file: RDB15904_A8Crc.seq
>D05305A2-1_A8Cr_BGH_rev2_F01_16_ABI24.ab1     1 TATGGGTACA GCACGGGGGA GGGGCAAACA ACAGATGGCT GGCAACTAGA AGGCACAGTC    61 GAGGCTGATC AGCGAGCTCT AGGAATTCTT ACTTTTTCTT TTTTGCCTGG CCGGCCTTTT   121 TCGTGGCCGC CGGCCTTTTG TCGCCTCCCA GCTGAGACAG GTCGATCCGT GTCTCGTACA   181 GGCCGGTGAT GCTCTGGTGG ATCAGGGTGG CGTCCAGCAC CTCTTTGGTG CTGGTGTACC   241 TCTTCCGGTC GATGGTGGTG TCAAAGTACT TGAAGGCGGC AGGGGCTCCC AGATTGGTCA   301 GGGTAAACAG GTGGATGATA TTCTCGGCCT GCTCTCTGAT GGGCTTATCC CGGTGCTTGT   361 TGTAGGCGGA CAGCACTTTG TCCAGATTAG CGTCGGCCAG GATCACTCTC TTGGAGAACT   421 CGCTGATCTG CTCGATGATC TCGTCCAGGT AGTGCTTGTG CTGTTCCACA AACAGCTGTT   481 TCTGCTCATT ATCCTCGGGG GAGCCCTTCA GCTTCTCATA GTGGCTGGCC AGGTACAGGA   541 AGTTCACATA TTTGGAGGGC AGGGCCAGTT CGTTTCCCTT CTGCAGTTCG CCGGCAGAGG   601 CCAGCATTCT CTTCCGGCCG TTTTCCAGCT CGAACAGGGA GTACTTAGGC AGCTTGATGA   661 TCAGGTCCTT TTTCACTTCT TTGTAGCCCT TGGCTTCCAG AAAGTCGATG GGATTCTTCT   721 CGAAGCTGCT TCTTTCCATG ATGGTGATCC CCAGCAGCTC TTTCACACTC TTCAGTTTCT   781 TGGACTTGCC CTTTTCCACT TTGGCCACCA CCAGCACAGA ATAGGCCACG GTGGGGCTGT   841 CGAAGCCGCC GTACTTCTTA GGGTCCCAGT CCTTCTTTCT GGCGATCAGC TTATCGCTGT   901 TCCTCTTGGG CAGGATAGAC TCTTTGCTGA AGCCGCCCTG TCTGCACCCT CGGTCTTTTT   961 TCACGATATT CACTTTGGGG GCATGCTCAG CACTTTCCGC CACGGTGCAA AATCCCGGCT 1021 GATATCCCAC ACGATCTCCC TGAATTCGAC CGATTTCTCG AATCAGAGGA CGCCT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl