Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pX330-Syk gRNA (#RDB15904)

Targeting construct of human spleen tyrosine kinase (Syk).

Clone info. Expression vector of gRNA for targeting human spleen tyrosine kinase (Syk) and human codon-optimized SpCas9.
Comment Effect of the targeting vecctor was confirmed by the depositor with immunoblotting.
Vector backbone pX330 (plasmid)
Size of vector backbone 8.5 kb
Selectable markers Amp^r
Gene/insert name Human SYK genomic DNA
Depositor|Developer Sada, Kiyonao |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Chihara, K et al. Sci Rep. 7(1) : 11480, 2017)
Remarks
The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
専用MTA（CRISPR/Cas9内包遺伝子材料専用 非営利学術目的）をお使いください [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Chihara, K et al. Sci Rep. 7(1) : 11480, 2017)。
備考
本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15904 pX330-Syk gRNA DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pX330-Syk gRNA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15904).

Reference section:

Chihara, K., Kato, Y., Yoshiki, H., Takeuchi, K., Fujieda, S., Sada, K., Syk-dependent tyrosine phosphorylation of 3BP2 is required for optimal FcRγ-mediated phagocytosis and chemokine expression in U937 cells. Sci. Rep. 7 (1): 11480 (2017). PMID 28904407. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15904_A8Crp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40pA3' element Reverse
Sequence file: RDB15904_A8Cra.seq check
>D05305A2-1_A8Cr_40pA3'elementRever_D01_10_ABI24.ab1
    1 TTTTAGGTGG GCGGACCTAT GGAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT
   61 TTTGCTGGCC TTTTGCTCAC ATGTGAGGGC CTATTTCCCA TGATTCCTTC ATATTTGCAT
  121 ATACGATACA AGGCTGTTAG AGAGATAATT GGAATTAATT TGACTGTAAA CACAAAGATA
  181 TTAGTACAAA ATACGTGACG TAGAAAGTAA TAATTTCTTG GGTAGTTTGC AGTTTTAAAA
  241 TTATGTTTTA AAATGGACTA TCATATGCTT ACCGTAACTT GAAAGTATTT CGATTTCTTG
  301 GCTTTATATA TCTTGTGGAA AGGACGAAAC ACCGTTTCGG CAACATCACC CGGGGTTTTA
  361 GAGCTAGAAA TAGCAAGTTA AAATAAGGCT AGTCCGTTAT CAACTTGAAA AAGTGGCACC
  421 GAGTCGGTGC TTTTTTGTTT TAGAGCTAGA AATAGCAAGT TAAAATAAGG CTAGTCCGTT
  481 TTTAGCGCGT GCGCCAATTC TGCAGACAAA TGGCTCTAGA GGTACCCGTT ACATAACTTA
  541 CGGTAAATGG CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAGTAA
  601 CGCCAATAGG GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT
  661 TGGCAGTACA TCAAGTGTAT CATATGCCAA GTACGCCCCC TATTGACGTC AATGACGGTA
  721 AATGGCCCGC CTGGCATTGT GCCCAGTACA TGACCTTATG GGACTTTCCT ACTTGGCAGT
  781 ACATCTACGT ATTAGTCATC GCTATTACCA TGGTCGAGGT GAGCCCCCAC GTTCTGCTTC
  841 ACTCTCCCCA TCTCCCCCCC CCCTCCCCAC CCCCCAATTT TTGTATTTTA TTTTATTTTT
901 TTTAATTTAT TTTTGTGCAG CCGATGGGGG GCGGGGGGGG GGGGGGGACG
//
Primer: RDB13535#1
Sequence file: RDB15904_A8Crb.seq check
>D05305A2-1_A8Cr_RDB13535#1_E01_13_ABI24.ab1
    1 TGACAAGGGC CTGAATCACT TTTTTTCAGG TTGGACCGGT GCCACCATGG ACTATAAGGA
   61 CCACGACGGA GCACTACAAG GATCATGATA TTGATTACAA AGACGATGAC GATAAGATGG
  121 CCCCAAAGAA GAAGCGGAAG GTCGGTATCC ACGGAGTCCC AGCAGCCGAC AAGAAGTACA
  181 GCATCGGCCT GGACATCGGC ACCAACTCTG TGGGCTGGGC CGTGATCACC GACGAGTACA
  241 AGGTGCCCAG CAAGAAATTC AAGGTGCTGG GCAACACCGA CCGGCACAGC ATCAAGAAGA
  301 ACCTGATCGG AGCCCTGCTG TTCGACAGCG GCGAAACAGC CGAGGCCACC CGGCTGAAGA
  361 GAACCGCCAG AAGAAGATAC ACCAGACGGA AGAACCGGAT CTGCTATCTG CAAGAGATCT
  421 TCAGCAACGA GATGGCCAAG GTGGACGACA GCTTCTTCCA CAGACTGGAA GAGTCCTTCC
  481 TGGTGGAAGA GGATAAGAAG CACGAGCGGC ACCCCATCTT CGGCAACATC GTGGACGAGG
  541 TGGCCTACCA CGAGAAGTAC CCCACCATCT ACCACCTGAG AAAGAAACTG GTGGACAGCA
  601 CCGACAGGCC GACCTGCGGC TGATCTATCT GGCCCTGGCC CACATGATCA AGTTCCGGGG
  661 CCACTTCCTG ATCGAGGGCG ACCTGAACCC CGACAACAGC GACGTGGACA AGCTGTTCAT
  721 CCAGCTGGTG CAGACCTACA ACCAGCTGTT CGAGGAAAAC CCCATCAACG CCAGCGGCGT
  781 GGACGCCCAA GGCCATCCTG TCTGCCAGAC TGAGCAAGAG CAGACGGCTG GGAAAATCTG
  841 ATCGGCCCAG CTGCCCGCCG AGAAGAAGAA TGGCCCTGTT CGGAAACCTG ATTGCCTGAG
  901 CCTGGGCCTG GACCCCCAAC TTCAGAACAC TTCGACCTGG TCGAGGATGC CAACCTGCAG
  961 CTGAGCAAGA CACCCTTACG AACGAACGAC TGGAACACCT GGCTGGCCGA ATCCGCGACA
1021 GATACGCGAA CTGTTCTGGC CGCAGACTGT CGAGTCATCC TGGCTGAACG G
//
Primer: BGH_rev
Sequence file: RDB15904_A8Crc.seq check
>D05305A2-1_A8Cr_BGH_rev2_F01_16_ABI24.ab1
    1 TATGGGTACA GCACGGGGGA GGGGCAAACA ACAGATGGCT GGCAACTAGA AGGCACAGTC
   61 GAGGCTGATC AGCGAGCTCT AGGAATTCTT ACTTTTTCTT TTTTGCCTGG CCGGCCTTTT
  121 TCGTGGCCGC CGGCCTTTTG TCGCCTCCCA GCTGAGACAG GTCGATCCGT GTCTCGTACA
  181 GGCCGGTGAT GCTCTGGTGG ATCAGGGTGG CGTCCAGCAC CTCTTTGGTG CTGGTGTACC
  241 TCTTCCGGTC GATGGTGGTG TCAAAGTACT TGAAGGCGGC AGGGGCTCCC AGATTGGTCA
  301 GGGTAAACAG GTGGATGATA TTCTCGGCCT GCTCTCTGAT GGGCTTATCC CGGTGCTTGT
  361 TGTAGGCGGA CAGCACTTTG TCCAGATTAG CGTCGGCCAG GATCACTCTC TTGGAGAACT
  421 CGCTGATCTG CTCGATGATC TCGTCCAGGT AGTGCTTGTG CTGTTCCACA AACAGCTGTT
  481 TCTGCTCATT ATCCTCGGGG GAGCCCTTCA GCTTCTCATA GTGGCTGGCC AGGTACAGGA
  541 AGTTCACATA TTTGGAGGGC AGGGCCAGTT CGTTTCCCTT CTGCAGTTCG CCGGCAGAGG
  601 CCAGCATTCT CTTCCGGCCG TTTTCCAGCT CGAACAGGGA GTACTTAGGC AGCTTGATGA
  661 TCAGGTCCTT TTTCACTTCT TTGTAGCCCT TGGCTTCCAG AAAGTCGATG GGATTCTTCT
  721 CGAAGCTGCT TCTTTCCATG ATGGTGATCC CCAGCAGCTC TTTCACACTC TTCAGTTTCT
  781 TGGACTTGCC CTTTTCCACT TTGGCCACCA CCAGCACAGA ATAGGCCACG GTGGGGCTGT
  841 CGAAGCCGCC GTACTTCTTA GGGTCCCAGT CCTTCTTTCT GGCGATCAGC TTATCGCTGT
  901 TCCTCTTGGG CAGGATAGAC TCTTTGCTGA AGCCGCCCTG TCTGCACCCT CGGTCTTTTT
  961 TCACGATATT CACTTTGGGG GCATGCTCAG CACTTTCCGC CACGGTGCAA AATCCCGGCT
1021 GATATCCCAC ACGATCTCCC TGAATTCGAC CGATTTCTCG AATCAGAGGA CGCCT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Chihara, K., Syk-dependent tyrosine phosphorylation of 3BP2 is required for optimal FcRγ-mediated phagocytosis and chemokine expression in U937 cells. Sci. Rep. 7 (1): 11480 (2017). PMID 28904407. [link to RRC of NBRP]