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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pX330-FcR gamma gRNA (#RDB15903)

Targeting construct of human Fc receptor gamma chain (FcR gamma).

Clone info. Expression vector of gRNA for targeting human Fc receptor gamma chain (FcR gamma) and human codon-optimized SpCas9.
Comment Effect of the targeting vecctor was confirmed by the depositor with immunoblotting.
Vector backbone pX330 (plasmid)
Size of vector backbone 8.5 kb
Selectable markers Amp^r
Gene/insert name Human FCER1G genomic DNA
Depositor|Developer Sada, Kiyonao |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Chihara, K et al. Sci Rep. 7(1) : 11480, 2017)
Remarks
The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
専用MTA（CRISPR/Cas9内包遺伝子材料専用 非営利学術目的）をお使いください [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Chihara, K et al. Sci Rep. 7(1) : 11480, 2017)。
備考
本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15903 pX330-FcR gamma gRNA DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pX330-FcR gamma gRNA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15903).

Reference section:

Chihara, K., Kato, Y., Yoshiki, H., Takeuchi, K., Fujieda, S., Sada, K., Syk-dependent tyrosine phosphorylation of 3BP2 is required for optimal FcRγ-mediated phagocytosis and chemokine expression in U937 cells. Sci. Rep. 7 (1): 11480 (2017). PMID 28904407. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15903_A8Crp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40pA3' element Reverse
Sequence file: RDB15903_A8Cra.seq check
>D05305A1-1_A8Cr_40pA3'elementRever_A01_01_ABI24.ab1
    1 GTTTGGGGGG GGGCGGGGCC TATGGAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG
   61 CCTTTTGCTG GCCTTTTGCT CACATGTGAG GGCCTATTTC CCATGATTCC TTCATATTTG
  121 CATATACGAT ACAAGGCTGT TAGAGAGATA ATTGGAATTA ATTTGACTGT AAACACAAAG
  181 ATATTAGTAC AAAATACGTG ACGTAGAAAG TAATAATTTC TTGGGTAGTT TGCAGTTTTA
  241 AAATTATGTT TTAAAATGGA CTATCATATG CTTACCGTAA CTTGAAAGTA TTTCGATTTC
  301 TTGGCTTTAT ATATCTTGTG GAAAGGACGA AACACCGAGT AAGAGCAAGA CCACTGCGTT
  361 TTAGAGCTAG AAATAGCAAG TTAAAATAAG GCTAGTCCGT TATCAACTTG AAAAAGTGGC
  421 ACCGAGTCGG TGCTTTTTTG TTTTAGAGCT AGAAATAGCA AGTTAAAATA AGGCTAGTCC
  481 GTTTTTAGCG CGTGCGCCAA TTCTGCAGAC AAATGGCTCT AGAGGTACCC GTTACATAAC
  541 TTACGGTAAA TGGCCCGCCT GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAG
  601 TAACGCCAAT AGGGACTTTC CATTGACGTC AATGGGTGGA GTATTTACGG TAAACTGCCC
  661 ACTTGGCAGT ACATCAAGTG TATCATATGC CAAGTACGCC CCCTATTGAC GTCAATGACG
  721 GTAAATGGCC CGCCTGGCAT TGTGCCCAGT ACATGACCTT ATGGGACTTT CCTACTTGGC
  781 AGTACATCTA CGTATTAGTC ATCGCTATTA CCATGGTCGA GGTGAGCCCC ACGTTCTGCT
  841 TCACTCTCCC CATCTCCCCC CCCCCTCCCC ACCCCCAATT TTGTATTTAT TTTATTTTTT
901 TAATTATTTT TGTGCAGCGA TGGGGGGCGG GGGGGGGGGG GGA
//
Primer: RDB13535#1
Sequence file: RDB15903_A8Crb.seq check
>D05305A1-1_A8Cr_RDB13535#1_B01_04_ABI24.ab1
    1 TGGGAATGCC TGAATCACTT TTTTTCAGGT TGGACCGGTG CCACCATGGA CTATAAGGAC
   61 CACGACGGAG ACTACAAGGA TCATGATATT GATTACAAAG ACGATGACGA TAAGATGGCC
  121 CCAAAGAAGA AGCGGAAGGT CGGTATCCAC GGAGTCCCAG CAGCCGACAA GAAGTACAGC
  181 ATCGGCCTGG ACATCGGCAC CAACTCTGTG GGCTGGGCCG TGATCACCGA CGAGTACAAG
  241 GTGCCCAGCA AGAAATTCAA GGTGCTGGGC AACACCGACC GGCACAGCAT CAAGAAGAAC
  301 CTGATCGGAG CCCTGCTGTT CGACAGCGGC GAAACAGCCG AGGCCACCCG GCTGAAGAGA
  361 ACCGCCAGAA GAAGATACAC CAGACGGAAG AACCGGATCT GCTATCTGCA AGAGATCTTC
  421 AGCAACGAGA TGGCCAAGGT GGACGACAGC TTCTTCCACA GACTGGAAGA GTCCTTCCTG
  481 GTGGAAGAGG ATAAGAAGCA CGAGCGGCAC CCCATCTTCG GCAACATCGT GGACGAGGTG
  541 GCCTACCACG AGAAGTACCC CACCATCTAC CACCTGAGAA AGAAACTGGT GGACAGCACC
  601 GACAAGGCCG ACCTGCGGCT GATCTATCTG GCCCTGGCCC ACATGATCAA GTTCCGGGGC
  661 CACTTCCTGA TCGAGGGCGA CCTGAACCCC GACAACAGCG ACGTGGACAA GCTGTTCATC
  721 CAGCTGGTGC AGACCTACAA CCAGCTGTTC GAGGAAAACC CCATCAACGC CAGCGGCGTG
  781 GACGCCAAGG CCATCCTGTC TGCCAGACTG AGCAAGAGCA GACGGCTGGA AAATCTGATC
  841 GCCCAGCTGC CCGGCGAGAA GAAGAATGGC CTGTTCGGAA ACCTGATTGC CCTGAGCCTG
  901 GGCCCTGACC CCCCAACTTC AAGAGCAACT TCGACCCTGT CGAGGATGCC CAAACTGCAG
  961 CTGAGCAGGA CACCTACGAC GAACGACTGC ACACCTGCTG GGCCAGATCG GCCGACAAGT
 1021 ACGGCGACCT GGCTTTCCTG ACGGACAGCA GCTGATCGAC GTCATCTTGC TTGAGCGACA
1081 TTCCATG
//
Primer: BGH_rev
Sequence file: RDB15903_A8Crc.seq check
>D05305A1-1_A8Cr_BGH_rev2_C01_07_ABI24.ab1
    1 CACGGGCTAG GGAGCACGGG GGAGGGGCAA ACAACAGATG GCTGGCAACT AGAAGGCACA
   61 GTCGAGGCTG ATCAGCGAGC TCTAGGAATT CTTACTTTTT CTTTTTTGCC TGGCCGGCCT
  121 TTTTCGTGGC CGCCGGCCTT TTGTCGCCTC CCAGCTGAGA CAGGTCGATC CGTGTCTCGT
  181 ACAGGCCGGT GATGCTCTGG TGGATCAGGG TGGCGTCCAG CACCTCTTTG GTGCTGGTGT
  241 ACCTCTTCCG GTCGATGGTG GTGTCAAAGT ACTTGAAGGC GGCAGGGGCT CCCAGATTGG
  301 TCAGGGTAAA CAGGTGGATG ATATTCTCGG CCTGCTCTCT GATGGGCTTA TCCCGGTGCT
  361 TGTTGTAGGC GGACAGCACT TTGTCCAGAT TAGCGTCGGC CAGGATCACT CTCTTGGAGA
  421 ACTCGCTGAT CTGCTCGATG ATCTCGTCCA GGTAGTGCTT GTGCTGTTCC ACAAACAGCT
  481 GTTTCTGCTC ATTATCCTCG GGGGAGCCCT TCAGCTTCTC ATAGTGGCTG GCCAGGTACA
  541 GGAAGTTCAC ATATTTGGAG GGCAGGGCCA GTTCGTTTCC CTTCTGCAGT TCGCCGGCAG
  601 AGGCCAGCAT TCTCTTCCGG CCGTTTTCCA GCTCGAACAG GGAGTACTTA GGCAGCTTGA
  661 TGATCAGGTC CTTTTTCACT TCTTTGTAGC CCTTGGCTTC CAGAAAGTCG ATGGGATTCT
  721 TCTCGAAGCT GCTTCTTTCC ATGATGGTGA TCCCCAGCAG CTCTTTCACA CTCTTCAGTT
  781 TCTTGGACTT GCCCTTTTCC ACTTTGGCCA CCACCAGCAC AGAATAGGCC ACGGTGGGGC
  841 TGTCGAAGCC GCCGTACTTC TTAGGGTCCC AGTCCTTCTT TCTGGCGATC AGCTTATCGC
  901 TGTTTCCTCT TGGGCAAGGA TAGACTCTTT GCTGAAAGCC GCCCTGTCTG CACCTCGGGT
  961 CTTTTTCACG ATATTCACTT TGGGGCATGC TCAGCACTTT CCCGCCACGG TGGCAAAATC
 1021 CCGGCCCTTA TCCCACACGA TCTCCCCGGA TTTCGACGAT TTGTTCTCCG AATCAGAAGG
1081 GCCGCCTTTC CGA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Chihara, K., Syk-dependent tyrosine phosphorylation of 3BP2 is required for optimal FcRγ-mediated phagocytosis and chemokine expression in U937 cells. Sci. Rep. 7 (1): 11480 (2017). PMID 28904407. [link to RRC of NBRP]