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pCAGGS1-CA-mmAhR (#RDB15879)

Expression vector of mouse constitutively active (CA)-aryl hydrocarbon receptor (AhR).

Clone info. Expression vector of mouse constitutively active (CA)-aryl hydrocarbon receptor (AhR) lacking much of the ligand-binding domain (277 to 418 aa), CAG promoter, see Fig. 1A of Kimura, E. et al., PLoS One., 12 (8): e0183497, 2017.
Comment A full-length AhR cDNA fragment was obtained from a mouse liver cDNA library. Expression was confirmed by the depositor with in utero electroporation and fluorescence microscopy.
Vector backbone pCAGGS (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Gene/insert name Mouse Ahr cDNA
Depositor|Developer Tohyama, Chiharu |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kimura, E et al. PLoS One 12 (8): e0183497, 2017).
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kimura, E et al. PLoS One 12 (8): e0183497, 2017)。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15879 pCAGGS1-CA-mmAhR DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCAGGS1-CA-mmAhR was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15879).

Reference section:

Kimura, E., Kubo, K.I., Endo, T., Ling, W., Nakajima, K., Kakeyama, M., Tohyama, C., Impaired dendritic growth and positioning of cortical pyramidal neurons by activation of aryl hydrocarbon receptor signaling in the developing mouse. PLoS One 12 (8): e0183497 (2017). PMID 28820910. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15879_A8Cmp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1
Sequence file: RDB15879_A8Cma.seq check
>D05162A2-1_A8Cm_pAxCA_F1_C01_03_ABI08.ab1
    1 CCCGCATGTT CCTACAATGT TTCATGCCTT CTTCTTTTTC CTACAGCTCC TGGGCAACGT
   61 GCTGGTTATT GTGCTGTCTC ATCATTTTGG CAAAGAATTC GGGATCCCGG AGCTCCGCTC
  121 GAGGCGGGCA CCATGAGCAG CGGCGCCAAC ATCACCTATG CCAGCCGCAA GCGGCGCAAG
  181 CCGGTGCAGA AAACAGTAAA GCCCATCCCC GCTGAAGGAA TTAAGTCAAA TCCTTCTAAG
  241 CGACACAGAG ACCGGCTGAA CACAGAGTTA GACCGCCTGG CCAGCCTGCT GCCCTTCCCG
  301 CAAGATGTTA TTAATAAGCT GGACAAACTC TCTGTTCTTA GGCTCAGCGT CAGCTACCTG
  361 AGGGCCAAGA GCTTCTTTGA TGTTGCATTA AAGTCCACCC CTGCTGACAG AAATGGAGGC
  421 CAGGACCAGT GTAGAGCACA AATCAGAGAC TGGCAGGATT TGCAAGAAGG AGAGTTCTTG
  481 TTACAGGCGC TGAATGGCTT TGTGCTGGTT GTCACAGCAG ATGCCTTGGT CTTCTATGCT
  541 TCCTCCACTA TCCAAGATTA CCTGGGCTTT CAGCAGTCTG ATGTCATCCA CCAGAGCGTA
  601 TATGAGCTCA TCCATACAGA AGACCGGGCG GAATTCCAGC GCCAGCTTCA CTGGGCTCTA
  661 AACCCAGACT CTGCACAAGG AGTGGACGAA GCCCATGGCC CTCCACAGGC AGCAGTCTAT
  721 TATACCCCAG ACCAGCTTCC TCCAGAGAAC GCTTCTTTCA TGGAGAGGTG CTTCAGGTGC
  781 CGGCTGAGGT GCCTGCTGGA TAATTCATCT GGTTTTCTGG CAATGAATTT CCAAGGGAGG
  841 TTAAAGTATC TTCATGGACA GAACAAGAAA GGGAAGGACG GAGCGCTGCT TCCTCCACAA
  901 CTGGCTTTGT TTGCAATAGC TACTCCACTT CAGCCACCCT CCATCCTGGA AATTCGAACC
  961 GGGGGATCGG GGGGATCCCC TACCAATACG CACCAAAGCA ACACTAGCCA GGAAAGACTG
 1021 GGGCTCCCCC AGTCAACCCC AGTAAAGGAA TTCTTTCCAC CCCCAGTTCT TCTTAATGAG
 1081 TTGCCTCATC CAAGCAGGAT GAGTCAATCT ATCTGTGTCC TTCCGATCAA AGCCCTGGCC
1141 GCCCTGTTAT AGAC
//
Primer: pCA-bgter
Sequence file: RDB15879_A8Cmb.seq check
>D05162A2-1_A8Cm_pCA-bgter_D01_04_ABI08.ab1
    1 ACCACGGAAT GTCCCAAAAA TTTTTGGCAG AGGGAAAAGA TCTCAGTGGT ATTTGTGAGC
   61 CAGGGCATTG GCCACACCAG CCACCACCTT CTGATAGGCA GCCTGCACCT GAGGAGTGAA
  121 TTAAGGAAAA AAGCGGCCGC AAAAGGAAAA CCGCTCGAGT CAACTCTGCA CCTTGCTTAG
  181 GAATGCCTGA GAACCTGGAA TTTCAGAGCT GTACTGCGTC TGGTCCACAG GGGTGCGCTG
  241 TGGCCCTGGC TGGCACTGAT ACATGGACAT GGCCCCAGCA TAGTATGCCT GAGGACTGAC
  301 CATGGCGGAC TGTGAGTTTA TCCCATGACT TTGGTTTTCA GGAACTTGTA AACAACTGAC
  361 AAAATCTAAA TTAGAAGTAG TGGGATGCAG GGACGGTTCA AAATCCCTTC CAGGGAAGTC
  421 CAACTGCACA CTCTTGGGAA TGTTCTGGAA GCAGAGGCTG ATTTACAGGG GAGCAAAAGT
  481 TCTGTGTGTA AGGCACACTG TCCACCTTCT GGGTTTGTAG GGAAATTTCC TGGAACAGTC
  541 CCCCTGTAAG CTGGAAAAAA ACTGAAAGTG CTTTAATTTC CTGCCTGGGG GACAGTTGAA
  601 AGAACCGGGA GGGGGGGGGT CCAACTTTGC AAACTGCCCG TGAATTTCCG GGGCTCCGAA
  661 CCCAAATCTT GGCGGGGGGC CCACCATCGA AAACAGCGGC TGCTGGGGGG CCCCAAACCC
  721 GGCGGCCGGG GGCCGCCTGA AATCGTTGCG GTTGCCCCTA AGGGCGGGGC TCCCGCAGCA
  781 TAAGCTTAAG GGTCTAACCC AAGCGTTCTG CTGGGCAACC CAAATTCCCA AAATTTAAAT
  841 TGTTCAGGGA AACCCCGGCG CAAGGTACAG AAATCTCCCC GTATAGCCGA TGATCTTTTA
  901 AATCACACTC CCCCGCCACG CGTGAGAGAC ACTTCTCGAG AAACTCTCTC GTGTCTCGAG
  961 GTGTCTGTAG ATATCCTCCA CAAATATCCC AGAGGTCTCC TCTAGATGCG TAGCGAATCA
 1021 CCGAGATTAA TAATATATTC AAAAAGAGAC CAGTCAATAC ATGCGCCCGC CGCGAGCGAT
1081 CGGAGTAGTC TGCATCAGAC GTCAGCTTCA TCTGATCAAT CCTAGTCTCA TG
//
Primer: RDB15879#1
Sequence file: RDB15879_A8Cmc.seq check
>D05162A2_1_A8Cm_RDB15879#1_G01_07_ABI08.ab1
    1 CAGGCCCAGC CGGTCTATTA TACCCCCAGA CCAGCTTCCT CCAGAGAACG CTTCTTTCAT
   61 GGAGAGGTGC TTCAGGTGCC GGCTGAGGTG CCTGCTGGAT AATTCATCTG GTTTTCTGGC
  121 AATGAATTTC CAAGGGAGGT TAAAGTATCT TCATGGACAG AACAAGAAAG GGAAGGACGG
  181 AGCGCTGCTT CCTCCACAAC TGGCTTTGTT TGCAATAGCT ACTCCACTTC AGCCACCCTC
  241 CATCCTGGAA ATTCGAACCG GGGGATCGGG GGATCCCCTA CCAATACGCA CCAAAAGCAA
  301 CACTAGCAGG AAAGACTGGG CTCCCCAGTC AACCCCAAGT AAGGATTCTT TCCACCCCAG
  361 TTCTCTTATG AGTGCCCTCA TCCAGCAGGA TGAGTCCATC TATCTGTGTC CTCCTTCAAG
  421 CCCTGCGCTG TTAGACAGCC ATTTTCTCAT GGGCTCCGTG AGCAAGTGCG GGAGTTGGCA
  481 AGACAGCTTT GCGGCCGCAG GAAGTGAGGC TGCGCTGAAA CATGAGCAAA TTGGCCATGC
  541 TCAGGACGTG AACCTTGCAC TCTCTGGCGG CCCCTCAGAG CTCTTTCCGG ATAATAAAAA
  601 TAATGACTTG TACAGCATCA TGAGGAACCT TGGGATTGAT TTTGAAGATA TCAGAAGCAT
  661 GCAGAACGAG GAGTTCTTCA GAACTGACTC CACCGCTGCT GGTGAGGTTG ACTTCAAAGA
  721 CATCGACATA ACGGACGAAA TCCTGACCTA CGTGCAGGAT TCCCTGAACA ATTCAACTTT
  781 GCTGAACTCG GCTTGCCAGC AGCAGCCTGT GACTCAGCAC CTAAGCTGTA TGCTGCAGGA
  841 GCGCCTGCAA CTAGAGCAAC ATGCAACAGC TTCAGCAGCC CCCCGCCGCA GGCTCTGGAG
  901 CCCCAGCAGC AGCTGTGTCA GATGGTGTGC CCCCCAGCAA GATCTGGGGT CCGAAGCACA
  961 CGGCAAATCA ACGGCACGTT TTGCAAGTTT GGAACCCCCA CCCCCTCCCG TGTCTTTCAA
 1021 CTGTCCCCAG CAGGACTAAA GCACTATCAG CTTCTTTTCA GCTTTACAGG GACTGCTCAT
1081 GAGTTACCCT ACAATCCAGA GGTGGGAACA CAG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kimura, E., Impaired dendritic growth and positioning of cortical pyramidal neurons by activation of aryl hydrocarbon receptor signaling in the developing mouse. PLoS One 12 (8): e0183497 (2017). PMID 28820910. [link to RRC of NBRP]