RDB15874 - pCSII-CMV-MCS _Y (YFP- type) c-GRAPHIC T2A H2B-Azurite

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pCSII-CMV-MCS _Y (YFP- type) c-GRAPHIC T2A H2B-Azurite (#RDB15874)

Lentiviral expression vector of GRAPHIC probe (LZB).

Clone info.	Lentiviral expression vector of GRAPHIC probe (GPI anchored Reconstitution-Activated Proteins Highlight Intercellular Connections), fluorescent indicator for intercellular contacts with optimized intercellular GFP reconstitution. This probe contains an mouse preproacrosin signal peptide (SP), superfolder GFP-CT (YFP type), basic leucine zipper domain (LZB), mouse Thy-1 GPI-anchored domain, 2A peptide, human histone 2B (H2B), Azurite, see Fig. 1 of Kinoshita, N. et al., iScience 15: 28-38, 2019.
Comment	Expression was confirmed by the depositor with detection of reconstituted YFP fluorescence and nucleic Azurite fluorecence.
Vector backbone	CSII-CMV-MCS (RDB04377) (plasmid)
Size of vector backbone	8.6 kb
Selectable markers	Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Gene/insert name	mouse Acr cDNA mouse Thy1 cDNA human histon H2BJ cDNA Aequorea victoria GFP cDNA
Depositor\|Developer	Shimogori, Tomomi \| Kinoshita, Nagatoki \|

Sequence (full)	RDB15874zzt01.seq compiled by us
Sequence (partial)	RDB15874pka01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose)[Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Kinoshita, N. et al., iScience 15: 28-38 (2019)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
Additional terms and conditions:
The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.

Remarks Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose)[Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Kinoshita, N. et al., iScience 15: 28-38 (2019)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. Additional terms and conditions: The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Remarks	Remember that you will be working with samples containing infectious virus.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利機関による非営利学術研究用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Kinoshita, N. et al., iScience 15: 28-38 (2019)) を引用し、謝辞の表明を必要とする。本件リソースの使用は学術機関での学術研究に限る。 MTAに書く付加的使用条件：レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15874	pCSII-CMV-MCS _Y (YFP- type) c-GRAPHIC T2A H2B-Azurite	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCSII-CMV-MCS _Y (YFP- type) c-GRAPHIC T2A H2B-Azurite was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15874).

Reference section:

Kinoshita, N., Huang, A.J.Y., McHugh, T.J., Suzuki, S.C., Masai, I., Kim, I.H., Soderling, S.H., Miyawaki, A., Shimogori, T., Genetically Encoded Fluorescent Indicator GRAPHIC Delineates Intercellular Connections. iScience 15: 28-38 (2019). PMID 31026667. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15874_A8Cdp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: D05101#1 (Pr0993) Region: insert 5', CMV pro_2 Sequence file: RDB15874_A8Cda.seq
>D05101A6_A8Cd_1_D05101#1_G02_20_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG GCGTTCTTCT TCTTNNGGGC TTGCAGCTTC 61 TTCTTGAGCT GGGCCATGGT GGCGCTAGCC GATCCGCCAC CGCCAGAGCC ACCTCCGCCT 121 GAACCGCCTC CACCTCTAGC CTTGTACAGC TCGTCCATGC CGAGAGTGAT CCCGGCGGCG 181 GTCACGAACT CCAGCAGGAC CATGTGATCG CGCTTCTCGT TGGGGTCTTT GCTCAGGACG 241 GTCTGGTAGC TCAGGTAGTG GTTGTCGGGC AGCAGCACGG GGCCGTCGCC GATGGGGGTG 301 TTCTGCTGGT AGTGGTCGGC GAGCTGCACG CTGCCGTCCT CGACGTTGTG GCGGACCGTG 361 AAGTTCGCCT TGATGCCGTT CTTTCTAGCC TGCAGCGTGG TGTTATCCTT GGCAACCACG 421 GACACTGCCA AGACCAGCAC AGCGACAGTT GGCAGCATCT CTACCATGGT GGCTCTAGCG 481 GATCTGACGG TTCACTAAAC CAGCTCTGCT TATATAGACC TNNNNNNGTA CACGCCTACC 541 GCCCATTTGC GTCAATGGGG CGGAGTTGTT ACGACATTTT GGAAAGTCCC GTTGATTTTG 601 GTGCCAAAAC AAACTCCCAT TGACGTCAAT GGGGTGGAGA CTTGGAAAAT CCCCGTGAGT 661 CNAANNCGCT ATCCACGCCC ATTGATGTAC TGCCNANNNN NNCATCANNN TGGTAATAGC 721 GATGACTAAT ACGTNNNATG NNACTGNCNN AGNANGAAAG TCCCNNNAAN NGGTCATGTA 781 CTNNNNATAA TGCCANGCGG NNCATTTNNC CGTCATNNNN NGTNCANTNN NGGGCGNACT 841 TNNCATATGN NNACACTTGA NGNNACTGNN NNNNNNNN //
Primer: PRE_rev (Pr0250) Region: PRE, insert 3' Sequence file: RDB15874_A8Cdb.seq
>D05101A6_1_A8Cd_PRE_rev_C01_03_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNANNNNNNN NNNATAGTTA AGANNACNNG TCAATCTTTC 61 NCAAATTTTG TAATCCAGAG GTTGATTATC GATAAGCTTG ATCCCTCGAT GTTAACTCTA 121 GATTACTTGT ACAGCTCGTC CATGCCGTGA GTGATCCCGG CGGCGGTGCG GAACTCCAGC 181 AGGACCATGT GATCGCGCTT CTCGTTGGGG TCTTTGCTCA GGGCGGACTG GGTGCTCAGG 241 TAGTGGTTGT CGGGCAGCAG CACGGGGCCG TCGCCGATGG GGGTGTTCTG CTGGTAGTGG 301 TCGGCGAGCT GCACGCTGCC GTCCTCGATG TTGTGGCGGA TCTTGAAGTT CACCTTGATG 361 CCGTTCTTCT GCTTGTCGGC CATGATATAG ATGTTGTGGC TGTTGAAGTT GTACTCCAGC 421 TTGTGCCCCA GGATGTTGCC GTCCTCCTTG AAGTCGATGC CCTTCAGCTC GATGCGGTTC 481 ACCAGGGTGT CGCCCTCGAA CTTCACCTCG GCGCGGGTCT TGTAGTTGCC GTCGTCCTTG 541 AAGAAGATGG TGCGCTCCTG GACGTANCCT TCGGGCATGG CGGACTTGAA GAAGTCGTGC 601 TGCTTCATGT GGTCGGGGTA GCGGCTGAAG CACTGCACGC CGTGGCTCAN GGTGGTCACG 661 ANGGTGGGCC AGGGCACGGG CAGCTTGCCG GTGGTGCAGA TGAACTTCAG GGTCAGCTTG 721 NCGTANGTGG CATCGCCCTC GCCCTCGCCG GACACGCTGA ACTTGTGGNC GTTTACGTCN 781 CCNTCCNGCT CGACCANGAT GGGNACCNCC CNNGTGAANC AGCTCCTCGC CCTTGCTCAT 841 GNATCCCTTA NCGCTGGTGN ACTNNGNGAT GNNNNNANTN NNNTCGGNNA CNGNCNNGCT 901 NNGNNNACTN CCNNNNNNNN CNNCNNNNNC ANNNNNNNCN GGATNNNCCN NNNNNNNNAN 961 NNNNNNNNN //
Primer: D05101#2 (Pr0994) Region: insert mid Sequence file: RDB15874_A8Cdc.seq
>D05101A6_A8Cd_1_D05101#2_H02_23_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GNNGNGGNGG NNCNGCNAGC GCCNCCATGG 61 CCNAGCTCNA GAAGAAGCTG CAAGCCNTGA AGAAGAAGAA CGCCCAGCTC AAGTGGAAGC 121 TCCAGGCCCT GAAGAAGAAG CTGGCCCAGA AGGCTAGCGG TGGAGGCGGT TCAGGCGGAG 181 GTGGCTCTGG CGGTGGCGGA TCGTCTAGCG GTGGAGGCGG TTCAGGCGGA GGTGGCTCTG 241 GCGGTGGCGG ATCGTCTAGC GGTGGAGGCG GTTCAGGCGG AGGTGGCTCT GGCGGTGGCG 301 GATCGTCTAG CTACCAACTC GAGAATGGCG GCATAAGCCT GCTGGTTCAG AACACATCCT 361 GGATGCTGCT GCTGCTGCTT TCCCTCTCCC TCCTCCAAGC CCTGGACTTC ATTTCTCTGT 421 CTAGCGAGGG CAGAGGAAGT CTTCTAACAT GCGGTGACGT CGAGGAGAAT CCTGGCCCAA 481 TGCCAGAGCC AGCGAAGTCT GCTCCCGCCC CGAAAAAGGG CTCCAAGAAG GCGGTGACTA 541 AGGCGCAGAA GAAAGGCGGC AAGAAGCGCA AGCGCAGCCG CAAGGAGAGC TATTCCATCT 601 ATGTGTACAA GGTTCTGAAG CAGGTCCACC CTGACACCGG CATTTCGTCC AAGGCCATGG 661 GCATCATGAA TTCGTTTGTG AACGACATTT TCGAGCGCAT CGCAGGTGAN GCTTCCCGCC 721 TGGCGCATTA CAACAAGCGC TCGACCATCA CCTCCAGGGA GATCCAGACG GCCGTGCGCC 781 TGCTGCTGCC TGGGGAGTTG GCCANNCACG CCGNGTCCGA GGGTACTAAN NNCATCACCA 841 AGTACACCAN CGCTAANGGA TCCATGANCA AGGGCGANGA NCTGTTCNCN NGGGGNGGNG 901 CCCATCCNGG NNCGAGCTGG ANNGCGNNGT AAANCNGNNN CNANGTNNAN CNNGTNCNNN 961 NAGGGNNAAN GGNNNNNGNN NACCTANNNN NNGCTGANNN NNNAANNNNN NNNNGNNNNN 1021 NNNNN //
Primer: EGFP-N_R2 (Pr0905) Region: insert mid Sequence file: RDB15874_A8Cdd.seq
>D05101A6_A8Cd_1_EGFP-N_R2_A03_03_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNCNCNCNNN NGCTCANGGN 61 GGTCACGNNG GNGGGCCAGG GCNNCGGGCA GCTTGCCGGT GGTGCAGATG AACTTCAGGG 121 TCAGCTTGCC GTAGGTGGCA TCGCCCTCGC CCTCGCCGGA CACGCTGAAC TTGTGGCCGT 181 TTACGTCGCC GTCCAGCTCG ACCAGGATGG GCACCACCCC GGTGAACAGC TCCTCGCCCT 241 TGCTCATGGA TCCCTTAGCG CTGGTGTACT TGGTGATGGC CTTAGTACCC TCGGACACGG 301 CGTGCTTGGC CAACTCCCCA GGCAGCAGCA GGCGCACGGC CGTCTGGATC TCCCTGGAGG 361 TGATGGTCGA GCGCTTGTTG TAATGCGCCA GGCGGGAAGC CTCACCTGCG ATGCGCTCGA 421 AAATGTCGTT CACAAACGAA TTCATGATGC CCATGGCCTT GGACGAAATG CCGGTGTCAG 481 GGTGGACCTG CTTCAGAACC TTGTACACAT AGATGGAATA GCTCTCCTTG CGGCTGCGCT 541 TGCGCTTCTT GCCGCCTTTC TTCTGCGCCT TAGTCACCGC CNNNNTGGAG CCCTTTTTCG 601 GGGCGGGAGC ANACTTCNCT GGCTCTGGCA TTGGGCCAGG ATTCTCCTCG ACGTCACCGC 661 ATGTTAGAAN ACTTCCTCTG CCCTCGCTAN ACAGANAAAN NGANGTCNNN GGCTTGNANG 721 AGGGANNAGG GAAANGNNNN CANCANCANC NTCCAGGANG TGNTCTGAAN NNNNNCANGC 781 TNATNNCCGC CNTTCTCNAG TTGGTAGCTA GACGATCCGN NNCCGNCAGA GCCACCTCCN 841 NNTGNNNCGC CTCNNNNNGC NAGACNATCC GCCACCGCCA NANCCNNCNT CCGNCCTGAN 901 NCGCCTNCAC NGCTAGNNGA TCCGNCNCCG CCAGANNCAC CNNCNGCCNG NANCNGNNNN 961 NACCGCNNNN NNNNTNNNGG GGNCANCTNN NTNNTTNNNN GGNCNNNGNN NNCNTNNNNN 1021 NNTTNNNNNN NNGGNNNNTT NNNTTNNNNN NN //
Primer: HIV-B_R (Pr0673) Region: HIV-1 psi, 5'LTR, CMV pro_1, CMV en_1 Sequence file: RDB15874_A8Cde.seq
>D05101A6_A8Cd_1_HIV-B_R_B03_06_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNTT TGGNGNANTC NNNNNNCGCC GCCCCTCGCC 61 TCTTGCCGTG CGCGCTTCNN CAAGCCGAGT CCTGCGTCGA GAGAGCTCCT CTGGTTTCCC 121 TTTCGCTTTC AAGTCCCTGT TCGGGCGCCA CTGCTAGAGA TTTTCCACAC TGACTAAAAG 181 GGTCTGAGGG ATCTCTAGTT ACCAGAGTCA CACAACAGAC GGGCACACAC TACTTGAAGC 241 ACTCAAGGCA AGCTTTATTG AGGCTTAAGC AGTGGGTTCC CTAGTTAGCC AGAGAGCTCC 301 CAGGCTCAGA TCTGGTCTAA CCAGAGAGAC CCAGTACAGG CAAAACGCGC TGCTTATATA 361 GACCTCCCAC CGTACACGCC TACCGCCCAT TTGCGTCAAT GGGGCGGAGT TGTTACGACA 421 TTTTGGAAAG TCCCGTTGAT TTTGGTGCCA AAACAAACTC CCATTGACGT CAATGGGGTG 481 GAGACTTGGA AATCCCCGTG AGTCAAACCG CTATCCACGC CCATTGATGT ACTGCCAAAA 541 CCGCATCACC ATGGTAATAG CGATGACTAA TACGTAGATG TACTGCCAAG TAGGAAAGTC 601 CCATAAGGTC ATGTACTGGG CATAATGCCA GGCGGGCCAT TTANCGTCAT TGACGTCAAT 661 AGGGGGCGTA CTTGGCATAT GATACACTTG ATGTACTGCC AAGTGGGCAG TTTACCGTAA 721 ATACTCCACC CATTGACGTC AATGGAAAGT CNCTATTGGC GTTACTATGG GGAACATACG 781 TCATTATTGA CGTCNATGGG CGGGGGTCGT NGGGCNGGTC AGCCAGGCGG NNCATTTANC 841 GTANNTTATG TAACGCGGAA CTNNNTATAT GGGCTATGAN CTAATGACCC CGNAATTGAT 901 TACTATNNAN NACNANNCNA NNANTCNANG NNNAACNNNN NNATCNGGNN NNNNNNTNNN 961 CGAANNNNNN NNAANNNNNN NNNN //
Primer: BGH_rev2 (Pr0606) Region: BGH pA, 3'LTR Sequence file: RDB15874_A8Cdf.seq
>D05101A6_A8Cd_1_BGH_rev2_E01_05_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNGGGNN NNNNNNNNNN NNNNGANGGC TGNNNNNNNG 61 AAGGCACNNN NNAGGCTGAT CAGCGGGTTT AAACGGGCCC TGCTAGAGAT TTTCCACACT 121 GACTAAAAGG GTCTGAGGGA TCTCTAGTTA CCAGAGTCAC ACAACAGACG GGCACACACT 181 ACTTGAAGCA CTCAAGGCAA GCTTTATTGA GGCTTAAGCA GTGGGTTCCC TAGTTAGCCA 241 GAGAGCTCCC AGGCTCAGAT CTGGTCTAAC CAGAGAGACC CAGTACAGTC CGGATGCAGC 301 TCTCGGGCCA TGTGATGAAA TGCTAGGCGG CTGTCAAACC TCCACTCTAA TACTTCTCTC 361 TCCGGGTCAT CCATCCCATG CAGGCTCACA GGGTGTAACA AGCGGGTGTT CTCTCCTTCA 421 TTGGCTTCTT CTACCTTCTC TTGCTCAACT GGTACTAGCT TGTAGCACCA TCCAAAGGTC 481 NNNGGATATC TGATCCCTGG CCCTGGTGTG TAGTTCTGCC AATCAGGGAA GTANCCTTGT 541 GTGTGGTAGA TCCNCAGATC AAGGATATCT TGTCTTCNTT GGGAGTGAAT TAGCCCTTCC 601 AGTCCCCCCT TTTCTTTTAA AAAGTGGCTN ANANCTACAG CTGCCTTGNN AGTCNNTGGN 661 CTTAAAGGNA NNTGANGNGT GACTGNNAAA CCCACCTCCT NCTNCNCTTG NGNTTCTANC 721 CNNGCNCNAN TCNNNNTNGG NANCTGCNGN ANNNGNNNCN TGTNATNGNT CNNNGTTTTN 781 NNNNNNNNN //
Primer: SV40pro_F (Pr0110) Region: EM7 pro, BleoR, SV40 pA Sequence file: RDB15874_A8Cdg.seq
>D05101A6_1_A8Cd_SV40pro_F_E01_05_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNGCTT TTTTGGNNNG CCNNNGNTTT 61 TGCAANANNN TCCCGGGAGC TTNNNNNNNN NNNNNNNNAT CTGATCAGCA CGTGTTGACA 121 ATTAATCATC GGCATAGTAT ATCGGCATAG TATAATACGA CAAGGTGAGG AACTAAACCA 181 TGGCCAAGTT GACCAGTGCC GTTCCGGTGC TCACCGCGCG CGACGTCGCC GGAGCGGTCG 241 AGTTCTGGAC CGACCGGCTC GGGTTCTCCC GGGACTTCGT GGAGGACGAC TTCGCCGGTG 301 TGGTCCGGGA CGACGTGACC CTGTTCATCA GCGCGGTCCA GGACCAGGTG GTGCCGGACA 361 ACACCCTGGC CTGGGTGTGG GTGCGCGGCC TGGACGAGCT GTACGCCGAG TGGTCGGAGG 421 TCGTGTCCAC GAACTTCCGG GACGCCTCCG GGCCGGCCAT GACCGAGATC GGCGAGCAGC 481 CGTGGGGGCG GGAGTTCGCC CTGCGCGACC CGGCCGGCAA CTGCGTGCAC TTCGTGGCCG 541 AGGAGCAGGA CTGACACGTG CTACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT 601 TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCNGCGC GGGGATCTCA 661 TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC TTATAATGGT TACNAATAAA 721 GCAATAGCAT CACAAATTTC ACNAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT 781 TTGTCCAAAC TCATCNATGT ATCTTATCAT GTCTGTATAC CGTCGANCTC TAGCTAGAGC 841 TNGGCGTAAT CATGGNCATA GCTGTTNCCT GNNTGAAANT GNTANNCGNN NACNATTNCN 901 NNNNNNATNN NNANNNGGNA NNNNNNNAAG NGNNAANNNN NNNNGNNNNC NNANNGANNN 961 NNNCNNANNN NNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Kinoshita, N., Genetically Encoded Fluorescent Indicator GRAPHIC Delineates Intercellular Connections. iScience 15: 28-38 (2019). PMID 31026667. [link to RRC of NBRP]