Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCSII-CMV-MCS _Y (YFP- type) c-GRAPHIC T2A H2B-Azurite

Lentiviral expression vector of GRAPHIC probe (LZB).

Sequence (full)	RDB15874zzt01.seq compiled by us
Sequence (partial)	RDB15874pka01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Kinoshita, N. et al., iScience 15: 28-38 (2019)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. Additional terms and conditions: The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word] This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
Remarks	Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Kinoshita, N. et al., iScience 15: 28-38 (2019)) を引用し、謝辞の表明を必要とする。本件リソースの使用は学術機関での学術研究に限る。 付加的提供条件： レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします （Schedule AとSchedule Bを入手[link])。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15874_A8Cdp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: D05101#1 Region: insert 5', CMV pro_2 Sequence file: RDB15874_A8Cda.seq
>D05101A6_A8Cd_1_D05101#1_G02_20_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG GCGTTCTTCT TCTTNNGGGC TTGCAGCTTC    61 TTCTTGAGCT GGGCCATGGT GGCGCTAGCC GATCCGCCAC CGCCAGAGCC ACCTCCGCCT   121 GAACCGCCTC CACCTCTAGC CTTGTACAGC TCGTCCATGC CGAGAGTGAT CCCGGCGGCG   181 GTCACGAACT CCAGCAGGAC CATGTGATCG CGCTTCTCGT TGGGGTCTTT GCTCAGGACG   241 GTCTGGTAGC TCAGGTAGTG GTTGTCGGGC AGCAGCACGG GGCCGTCGCC GATGGGGGTG   301 TTCTGCTGGT AGTGGTCGGC GAGCTGCACG CTGCCGTCCT CGACGTTGTG GCGGACCGTG   361 AAGTTCGCCT TGATGCCGTT CTTTCTAGCC TGCAGCGTGG TGTTATCCTT GGCAACCACG   421 GACACTGCCA AGACCAGCAC AGCGACAGTT GGCAGCATCT CTACCATGGT GGCTCTAGCG   481 GATCTGACGG TTCACTAAAC CAGCTCTGCT TATATAGACC TNNNNNNGTA CACGCCTACC   541 GCCCATTTGC GTCAATGGGG CGGAGTTGTT ACGACATTTT GGAAAGTCCC GTTGATTTTG   601 GTGCCAAAAC AAACTCCCAT TGACGTCAAT GGGGTGGAGA CTTGGAAAAT CCCCGTGAGT   661 CNAANNCGCT ATCCACGCCC ATTGATGTAC TGCCNANNNN NNCATCANNN TGGTAATAGC   721 GATGACTAAT ACGTNNNATG NNACTGNCNN AGNANGAAAG TCCCNNNAAN NGGTCATGTA   781 CTNNNNATAA TGCCANGCGG NNCATTTNNC CGTCATNNNN NGTNCANTNN NGGGCGNACT 841 TNNCATATGN NNACACTTGA NGNNACTGNN NNNNNNNN //
Primer: PRE_rev Region: PRE, insert 3' Sequence file: RDB15874_A8Cdb.seq
>D05101A6_1_A8Cd_PRE_rev_C01_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNANNNNNNN NNNATAGTTA AGANNACNNG TCAATCTTTC    61 NCAAATTTTG TAATCCAGAG GTTGATTATC GATAAGCTTG ATCCCTCGAT GTTAACTCTA   121 GATTACTTGT ACAGCTCGTC CATGCCGTGA GTGATCCCGG CGGCGGTGCG GAACTCCAGC   181 AGGACCATGT GATCGCGCTT CTCGTTGGGG TCTTTGCTCA GGGCGGACTG GGTGCTCAGG   241 TAGTGGTTGT CGGGCAGCAG CACGGGGCCG TCGCCGATGG GGGTGTTCTG CTGGTAGTGG   301 TCGGCGAGCT GCACGCTGCC GTCCTCGATG TTGTGGCGGA TCTTGAAGTT CACCTTGATG   361 CCGTTCTTCT GCTTGTCGGC CATGATATAG ATGTTGTGGC TGTTGAAGTT GTACTCCAGC   421 TTGTGCCCCA GGATGTTGCC GTCCTCCTTG AAGTCGATGC CCTTCAGCTC GATGCGGTTC   481 ACCAGGGTGT CGCCCTCGAA CTTCACCTCG GCGCGGGTCT TGTAGTTGCC GTCGTCCTTG   541 AAGAAGATGG TGCGCTCCTG GACGTANCCT TCGGGCATGG CGGACTTGAA GAAGTCGTGC   601 TGCTTCATGT GGTCGGGGTA GCGGCTGAAG CACTGCACGC CGTGGCTCAN GGTGGTCACG   661 ANGGTGGGCC AGGGCACGGG CAGCTTGCCG GTGGTGCAGA TGAACTTCAG GGTCAGCTTG   721 NCGTANGTGG CATCGCCCTC GCCCTCGCCG GACACGCTGA ACTTGTGGNC GTTTACGTCN   781 CCNTCCNGCT CGACCANGAT GGGNACCNCC CNNGTGAANC AGCTCCTCGC CCTTGCTCAT   841 GNATCCCTTA NCGCTGGTGN ACTNNGNGAT GNNNNNANTN NNNTCGGNNA CNGNCNNGCT   901 NNGNNNACTN CCNNNNNNNN CNNCNNNNNC ANNNNNNNCN GGATNNNCCN NNNNNNNNAN 961 NNNNNNNNN //
Primer: D05101#2 Region: insert mid Sequence file: RDB15874_A8Cdc.seq
>D05101A6_A8Cd_1_D05101#2_H02_23_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GNNGNGGNGG NNCNGCNAGC GCCNCCATGG    61 CCNAGCTCNA GAAGAAGCTG CAAGCCNTGA AGAAGAAGAA CGCCCAGCTC AAGTGGAAGC   121 TCCAGGCCCT GAAGAAGAAG CTGGCCCAGA AGGCTAGCGG TGGAGGCGGT TCAGGCGGAG   181 GTGGCTCTGG CGGTGGCGGA TCGTCTAGCG GTGGAGGCGG TTCAGGCGGA GGTGGCTCTG   241 GCGGTGGCGG ATCGTCTAGC GGTGGAGGCG GTTCAGGCGG AGGTGGCTCT GGCGGTGGCG   301 GATCGTCTAG CTACCAACTC GAGAATGGCG GCATAAGCCT GCTGGTTCAG AACACATCCT   361 GGATGCTGCT GCTGCTGCTT TCCCTCTCCC TCCTCCAAGC CCTGGACTTC ATTTCTCTGT   421 CTAGCGAGGG CAGAGGAAGT CTTCTAACAT GCGGTGACGT CGAGGAGAAT CCTGGCCCAA   481 TGCCAGAGCC AGCGAAGTCT GCTCCCGCCC CGAAAAAGGG CTCCAAGAAG GCGGTGACTA   541 AGGCGCAGAA GAAAGGCGGC AAGAAGCGCA AGCGCAGCCG CAAGGAGAGC TATTCCATCT   601 ATGTGTACAA GGTTCTGAAG CAGGTCCACC CTGACACCGG CATTTCGTCC AAGGCCATGG   661 GCATCATGAA TTCGTTTGTG AACGACATTT TCGAGCGCAT CGCAGGTGAN GCTTCCCGCC   721 TGGCGCATTA CAACAAGCGC TCGACCATCA CCTCCAGGGA GATCCAGACG GCCGTGCGCC   781 TGCTGCTGCC TGGGGAGTTG GCCANNCACG CCGNGTCCGA GGGTACTAAN NNCATCACCA   841 AGTACACCAN CGCTAANGGA TCCATGANCA AGGGCGANGA NCTGTTCNCN NGGGGNGGNG   901 CCCATCCNGG NNCGAGCTGG ANNGCGNNGT AAANCNGNNN CNANGTNNAN CNNGTNCNNN   961 NAGGGNNAAN GGNNNNNGNN NACCTANNNN NNGCTGANNN NNNAANNNNN NNNNGNNNNN 1021 NNNNN //
Primer: EGFP-N_R2 Region: insert mid Sequence file: RDB15874_A8Cdd.seq
>D05101A6_A8Cd_1_EGFP-N_R2_A03_03_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNCNCNCNNN NGCTCANGGN    61 GGTCACGNNG GNGGGCCAGG GCNNCGGGCA GCTTGCCGGT GGTGCAGATG AACTTCAGGG   121 TCAGCTTGCC GTAGGTGGCA TCGCCCTCGC CCTCGCCGGA CACGCTGAAC TTGTGGCCGT   181 TTACGTCGCC GTCCAGCTCG ACCAGGATGG GCACCACCCC GGTGAACAGC TCCTCGCCCT   241 TGCTCATGGA TCCCTTAGCG CTGGTGTACT TGGTGATGGC CTTAGTACCC TCGGACACGG   301 CGTGCTTGGC CAACTCCCCA GGCAGCAGCA GGCGCACGGC CGTCTGGATC TCCCTGGAGG   361 TGATGGTCGA GCGCTTGTTG TAATGCGCCA GGCGGGAAGC CTCACCTGCG ATGCGCTCGA   421 AAATGTCGTT CACAAACGAA TTCATGATGC CCATGGCCTT GGACGAAATG CCGGTGTCAG   481 GGTGGACCTG CTTCAGAACC TTGTACACAT AGATGGAATA GCTCTCCTTG CGGCTGCGCT   541 TGCGCTTCTT GCCGCCTTTC TTCTGCGCCT TAGTCACCGC CNNNNTGGAG CCCTTTTTCG   601 GGGCGGGAGC ANACTTCNCT GGCTCTGGCA TTGGGCCAGG ATTCTCCTCG ACGTCACCGC   661 ATGTTAGAAN ACTTCCTCTG CCCTCGCTAN ACAGANAAAN NGANGTCNNN GGCTTGNANG   721 AGGGANNAGG GAAANGNNNN CANCANCANC NTCCAGGANG TGNTCTGAAN NNNNNCANGC   781 TNATNNCCGC CNTTCTCNAG TTGGTAGCTA GACGATCCGN NNCCGNCAGA GCCACCTCCN   841 NNTGNNNCGC CTCNNNNNGC NAGACNATCC GCCACCGCCA NANCCNNCNT CCGNCCTGAN   901 NCGCCTNCAC NGCTAGNNGA TCCGNCNCCG CCAGANNCAC CNNCNGCCNG NANCNGNNNN   961 NACCGCNNNN NNNNTNNNGG GGNCANCTNN NTNNTTNNNN GGNCNNNGNN NNCNTNNNNN 1021 NNTTNNNNNN NNGGNNNNTT NNNTTNNNNN NN //
Primer: HIV-B_R Region: HIV-1 psi, 5'LTR, CMV pro_1, CMV en_1 Sequence file: RDB15874_A8Cde.seq
>D05101A6_A8Cd_1_HIV-B_R_B03_06_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNTT TGGNGNANTC NNNNNNCGCC GCCCCTCGCC    61 TCTTGCCGTG CGCGCTTCNN CAAGCCGAGT CCTGCGTCGA GAGAGCTCCT CTGGTTTCCC   121 TTTCGCTTTC AAGTCCCTGT TCGGGCGCCA CTGCTAGAGA TTTTCCACAC TGACTAAAAG   181 GGTCTGAGGG ATCTCTAGTT ACCAGAGTCA CACAACAGAC GGGCACACAC TACTTGAAGC   241 ACTCAAGGCA AGCTTTATTG AGGCTTAAGC AGTGGGTTCC CTAGTTAGCC AGAGAGCTCC   301 CAGGCTCAGA TCTGGTCTAA CCAGAGAGAC CCAGTACAGG CAAAACGCGC TGCTTATATA   361 GACCTCCCAC CGTACACGCC TACCGCCCAT TTGCGTCAAT GGGGCGGAGT TGTTACGACA   421 TTTTGGAAAG TCCCGTTGAT TTTGGTGCCA AAACAAACTC CCATTGACGT CAATGGGGTG   481 GAGACTTGGA AATCCCCGTG AGTCAAACCG CTATCCACGC CCATTGATGT ACTGCCAAAA   541 CCGCATCACC ATGGTAATAG CGATGACTAA TACGTAGATG TACTGCCAAG TAGGAAAGTC   601 CCATAAGGTC ATGTACTGGG CATAATGCCA GGCGGGCCAT TTANCGTCAT TGACGTCAAT   661 AGGGGGCGTA CTTGGCATAT GATACACTTG ATGTACTGCC AAGTGGGCAG TTTACCGTAA   721 ATACTCCACC CATTGACGTC AATGGAAAGT CNCTATTGGC GTTACTATGG GGAACATACG   781 TCATTATTGA CGTCNATGGG CGGGGGTCGT NGGGCNGGTC AGCCAGGCGG NNCATTTANC   841 GTANNTTATG TAACGCGGAA CTNNNTATAT GGGCTATGAN CTAATGACCC CGNAATTGAT   901 TACTATNNAN NACNANNCNA NNANTCNANG NNNAACNNNN NNATCNGGNN NNNNNNTNNN 961 CGAANNNNNN NNAANNNNNN NNNN //
Primer: BGH_rev2 Region: BGH pA, 3'LTR Sequence file: RDB15874_A8Cdf.seq
>D05101A6_A8Cd_1_BGH_rev2_E01_05_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNGGGNN NNNNNNNNNN NNNNGANGGC TGNNNNNNNG    61 AAGGCACNNN NNAGGCTGAT CAGCGGGTTT AAACGGGCCC TGCTAGAGAT TTTCCACACT   121 GACTAAAAGG GTCTGAGGGA TCTCTAGTTA CCAGAGTCAC ACAACAGACG GGCACACACT   181 ACTTGAAGCA CTCAAGGCAA GCTTTATTGA GGCTTAAGCA GTGGGTTCCC TAGTTAGCCA   241 GAGAGCTCCC AGGCTCAGAT CTGGTCTAAC CAGAGAGACC CAGTACAGTC CGGATGCAGC   301 TCTCGGGCCA TGTGATGAAA TGCTAGGCGG CTGTCAAACC TCCACTCTAA TACTTCTCTC   361 TCCGGGTCAT CCATCCCATG CAGGCTCACA GGGTGTAACA AGCGGGTGTT CTCTCCTTCA   421 TTGGCTTCTT CTACCTTCTC TTGCTCAACT GGTACTAGCT TGTAGCACCA TCCAAAGGTC   481 NNNGGATATC TGATCCCTGG CCCTGGTGTG TAGTTCTGCC AATCAGGGAA GTANCCTTGT   541 GTGTGGTAGA TCCNCAGATC AAGGATATCT TGTCTTCNTT GGGAGTGAAT TAGCCCTTCC   601 AGTCCCCCCT TTTCTTTTAA AAAGTGGCTN ANANCTACAG CTGCCTTGNN AGTCNNTGGN   661 CTTAAAGGNA NNTGANGNGT GACTGNNAAA CCCACCTCCT NCTNCNCTTG NGNTTCTANC   721 CNNGCNCNAN TCNNNNTNGG NANCTGCNGN ANNNGNNNCN TGTNATNGNT CNNNGTTTTN 781 NNNNNNNNN //
Primer: SV40pro_F Region: EM7 pro, BleoR, SV40 pA Sequence file: RDB15874_A8Cdg.seq
>D05101A6_1_A8Cd_SV40pro_F_E01_05_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNGCTT TTTTGGNNNG CCNNNGNTTT    61 TGCAANANNN TCCCGGGAGC TTNNNNNNNN NNNNNNNNAT CTGATCAGCA CGTGTTGACA   121 ATTAATCATC GGCATAGTAT ATCGGCATAG TATAATACGA CAAGGTGAGG AACTAAACCA   181 TGGCCAAGTT GACCAGTGCC GTTCCGGTGC TCACCGCGCG CGACGTCGCC GGAGCGGTCG   241 AGTTCTGGAC CGACCGGCTC GGGTTCTCCC GGGACTTCGT GGAGGACGAC TTCGCCGGTG   301 TGGTCCGGGA CGACGTGACC CTGTTCATCA GCGCGGTCCA GGACCAGGTG GTGCCGGACA   361 ACACCCTGGC CTGGGTGTGG GTGCGCGGCC TGGACGAGCT GTACGCCGAG TGGTCGGAGG   421 TCGTGTCCAC GAACTTCCGG GACGCCTCCG GGCCGGCCAT GACCGAGATC GGCGAGCAGC   481 CGTGGGGGCG GGAGTTCGCC CTGCGCGACC CGGCCGGCAA CTGCGTGCAC TTCGTGGCCG   541 AGGAGCAGGA CTGACACGTG CTACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT   601 TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCNGCGC GGGGATCTCA   661 TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC TTATAATGGT TACNAATAAA   721 GCAATAGCAT CACAAATTTC ACNAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT   781 TTGTCCAAAC TCATCNATGT ATCTTATCAT GTCTGTATAC CGTCGANCTC TAGCTAGAGC   841 TNGGCGTAAT CATGGNCATA GCTGTTNCCT GNNTGAAANT GNTANNCGNN NACNATTNCN   901 NNNNNNATNN NNANNNGGNA NNNNNNNAAG NGNNAANNNN NNNNGNNNNC NNANNGANNN 961 NNNCNNANNN NNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.10.12

GNP_filter3_RDBDEP_html_221010.pl