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pCSII-CMV-MCS _c-GRAPHIC T2A H2B-Azurite

Lentiviral expression vector of GRAPHIC probe (LZB).

Catalog number RDB15873
Resource name pCSII-CMV-MCS _c-GRAPHIC T2A H2B-Azurite
Clone info. Lentiviral expression vector of GRAPHIC probe (GPI anchored Reconstitution-Activated Proteins Highlight Intercellular Connections), fluorescent indicator for intercellular contacts with optimized intercellular GFP reconstitution. This probe contains an mouse preproacrosin signal peptide (SP), superfolder GFP-CT, basic leucine zipper domain (LZB), mouse Thy-1 GPI-anchored domain, 2A peptide, human histone 2B (H2B), Azurite, see Fig. 1 of Kinoshita, N. et al., iScience 15: 28-38, 2019.
Expression was confirmed by the depositor with detection of reconstituted GFP fluorescence and nucleic Azurite fluorecence. Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-CMV-MCS (plasmid)
Size of vector backbone 8.6 kb
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Gene/insert name mouse Acr cDNA mouse Thy1 cDNA human histon H2BJ cDNA Aequorea victoria GFP cDNA
Depositor|Developer Shimogori, Tomomi | Kinoshita, Nagatoki |
Other clones in our bank

External Database
mouse Acr mouse Thy1 human histon H2BJ Aequorea victoria GFP

      Reference sequence
        

      Distribution information

      Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
      Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Kinoshita, N. et al., iScience 15: 28-38 (2019)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
      Additional terms and conditions for distribution The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
      Ordering Please visit Information of Request for Distribution.[link] 
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      Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
      Schedule A (GFP-A) [Link]
      Remarks Remember that you will be working with samples containing infectious virus.
      Please use Schedule A (GFP-A) additionally.
      提供案内 (日本国内) [open/close]

      提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Kinoshita, N. et al., iScience 15: 28-38 (2019)) を引用し、謝辞の表明を必要とする。本件リソースの使用は学術機関での学術研究に限る。
      付加的提供条件 レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
      提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
      提供依頼書 [Word]
      提供同意書 (非営利機関による非営利学術研究用)[Word]
      Schedule A(GFP-A, 英語版のみ) [Link]
      備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
      Schedule A(GFP-A, 英語版のみ)が追加で必要です。

      Catalog # Resource name Shipping form Fee (non-profit org.)
      RDB15873 pCSII-CMV-MCS _c-GRAPHIC T2A H2B-Azurite DNA solution

      Ordering Information [in Japanese] [in English]
      Please review the QC test results indicated by check icon below as well as clone information before placing your order.

      References and tips

      Electronic file

      Sequence (partial) RDB15873pka01.seq provided by the depositor
      Sequence (full) RDB15873zzt01.seq compiled by us

      Original reference

      original Kinoshita, N., Genetically Encoded Fluorescent Indicator GRAPHIC Delineates Intercellular Connections. iScience 15: 28-38 (2019). PMID 31026667.

      Further references such as user reports and related articles (go to bottom)

      Featured content


      Sequence information

      RIKEN BRC has sequenced portions of this material for quality test.
      Please review the QC test results indicated by check icon as well as clone information before placing your order.

      Test sheet RDB15873_A8Cdp1-1.pdf check

      Nucleotide sequence of a portion of this resource (if available).

      Primer: D05101#1
      Region: insert 5', CMV pro_2
      Sequence file: RDB15873_A8Cda.seq check
      >D05101A5_A8Cd_1_D05101#1_B02_05_ABI24.ab1
          1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNGGCGTTC TTCTTCTTCN GGGCTTGCAG
         61 CTTCTTCTTG AGCTGGGCCA TGGTGGCGCT AGCCGATCCG CCACCGCCAG AGCCACCTCC
        121 GCCTGAACCG CCTCCACCTC TAGCCTTGTA CAGCTCGTCC ATGCCGAGAG TGATCCCGGC
        181 GGCGGTCACG AACTCCAGCA GGACCATGTG ATCGCGCTTC TCGTTGGGGT CTTTGCTCAG
        241 GACGGTCTGG GTGCTCAGGT AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT CGCCGATGGG
        301 GGTGTTCTGC TGGTAGTGGT CGGCGAGCTG CACGCTGCCG TCCTCGACGT TGTGGCGGAC
        361 CGTGAAGTTC GCCTTGATGC CGTTCTTTCT AGCCTGCAGC GTGGTGTTAT CCTTGGCAAC
        421 CACGGACACT GCCAAGACCA GCACAGCGAC AGTTGGCAGC ATCTCTACCA TGGTGGCTCT
        481 AGCGGATCTG ACGGTTCACT AAACCAGCTC TGCTTATATA GACCTCCCAC CGTACACGCC
        541 TACCGCCCAT TTGCGTCAAT GGGGCGGAGT TGTTACGACA TTTTGGAAAG TCCCGTTGAT
        601 TTTGGTGCCA AAACAAACTC CCATTGACGT CAATGGGGTG GAGACTTGGA AATCCCCGTG
        661 AGTCAAACCG CTATCCACGC CCATTGATGT ACTGCCNAAA ACCNNCATCA CCATGGTAAT
        721 AGCGATGACT AATACGTANA TGTACTGNNC NAANTANGAA AGTCCCATAA NGTCATGTAC
        781 TGNGCATAAT GCCNNNNGGG CCATTTNCCN NTCNTTGACG TCNANTNNNN GGGCGNACTN
        841 NNCATATGAT ACACNTTGAT GTACTGCNNA NNNGGGGCAN NTTTACCGTN NANACNNCNA
        901 CCCNNNNTNG ANNNNNNANT GGNNAAAGNN NCCCTNNNNN NNGNTNNNTN NNNGGNNNNN
      961 NNTNNNNNNN NNTNANNNNN NNNGNNNNNN NNGGNNNNNN NNNN
      //
      Primer: D05101#2
      Region: insert mid
      Sequence file: RDB15873_A8Cdb.seq check
      >D05101A5_A8Cd_1_D05101#2_C02_08_ABI24.ab1
          1 NNNNNNNNNN NNNNNNNNNN NGCNNNNGNG GNNGCGGNNC NGCTAGCGCC NCCNTGGCCC
         61 AGCTCAAGAA GAAGCTGCAA GCCCTGAAGA AGAAGAACGC CCAGCTCAAG TGGAAGCTCC
        121 AGGCCCTGAA GAAGAAGCTG GCCCAGAAGG CTAGCGGTGG AGGCGGTTCA GGCGGAGGTG
        181 GCTCTGGCGG TGGCGGATCG TCTAGCGGTG GAGGCGGTTC AGGCGGAGGT GGCTCTGGCG
        241 GTGGCGGATC GTCTAGCGGT GGAGGCGGTT CAGGCGGAGG TGGCTCTGGC GGTGGCGGAT
        301 CGTCTAGCTA CCAACTCGAG AATGGCGGCA TAAGCCTGCT GGTTCAGAAC ACATCCTGGA
        361 TGCTGCTGCT GCTGCTTTCC CTCTCCCTCC TCCAAGCCCT GGACTTCATT TCTCTGTCTA
        421 GCGAGGGCAG AGGAAGTCTT CTAACATGCG GTGACGTCGA GGAGAATCCT GGCCCAATGC
        481 CAGAGCCAGC GAAGTCTGCT CCCGCCCCGA AAAAGGGCTC CAAGAAGGCG GTGACTAAGG
        541 CGCAGAAGAA AGGCGGCAAG AAGCGCAAGC GCAGCCGCAA GGAGAGCTAT TCCATCTATG
        601 TGTACAAGGT TCTGAAGCAG GTCCACCCTG ACACCGGCAT TTCGTCCAAG GCCATGGGCA
        661 TCATGAATTC GTTTGTGAAC GACATTTTCG AGCGCATCGC AGGTGAGGCT TCCCGCCTGG
        721 CGCATTACAA CAAGCGCTCG ACCATCACCT CCAGGGANAT CCAGACGGCC GTGCGCCTGC
        781 TGCTGCCTGG GGGAGTTGGN CAAGCACGCC GNGTCCGANG GTACTAAGGC CATCACCNAG
        841 TACACCAGCG CTNANGNNNC CATGANCAAN GNCGANGNNC TGTTCANCGG GGGNGGNGCC
        901 CATCCNNGNN CGANCTGGNN GGCGNNGNNA ACGGNCNCNA NNTNNANCGN GNNCNNNNGA
      961 NGNCNANGNN NANGNNNNCT ANNNNNNNNN N
      //
      Primer: EGFP-N_R2
      Region: insert mid
      Sequence file: RDB15873_A8Cdc.seq check
      >D05101A5_A8Cd_1_EGFP-N_R2_D02_11_ABI24.ab1
          1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNCNNG CCNNNGCTCN NGGNGGTCAC
         61 GANGGNGGGC CAGGGCACGG GCAGCTTGCC GGTGGTGCAG ATGAACTTCA GGGTCAGCTT
        121 GCCGTAGGTG GCATCGCCCT CGCCCTCGCC GGACACGCTG AACTTGTGGC CGTTTACGTC
        181 GCCGTCCAGC TCGACCAGGA TGGGCACCAC CCCGGTGAAC AGCTCCTCGC CCTTGCTCAT
        241 GGATCCCTTA GCGCTGGTGT ACTTGGTGAT GGCCTTAGTA CCCTCGGACA CGGCGTGCTT
        301 GGCCAACTCC CCAGGCAGCA GCAGGCGCAC GGCCGTCTGG ATCTCCCTGG AGGTGATGGT
        361 CGAGCGCTTG TTGTAATGCG CCAGGCGGGA AGCCTCACCT GCGATGCGCT CGAAAATGTC
        421 GTTCACAAAC GAATTCATGA TGCCCATGGC CTTGGACGAA ATGCCGGTGT CAGGGTGGAC
        481 CTGCTTCAGA ACCTTGTACA CATAGATGGA ATAGCTCTCC TTGCGGCTGC GCTTGCGCTT
        541 CTTGCCGCCT TTCTTCTGCG CCTTAGTCAC CGCCTTCTTG GAGCCCTTTT TCGGGGCGGG
        601 AGCAGACTTC GCTGGCTCTG GCATTGGGCC AGGATTCTCC TCGACGTCAC CGCATGTTAG
        661 AAGACTTCCT CTGCCCTCGC TAGACAGAGA AATGAAGTCC AGGGCTTGGA GGANGGAGAG
        721 GGAAAGCAGC AGCAGCAGCA TCCAGGATGT GTTCTGAACC AGCAGGCTTA TGCCGCCATT
        781 CTCGAGTTGG TAGCTAGACG ATCCGCCACC GCCAGANCCA NCTCCGCCTG ANCCGCCTCC
        841 ACCGCTAGAC GATNCGNCNN CGCCAGANCC ACCTCCGCCN GAACCGCCTN NNNCGNNNGA
        901 CGATNCGCCA CNNCCNGANC CNCNNCCNNC NNGNNNNNNN NNCNCCGCNA GNCNTTCNGG
      961 NNNNNCNTNC NTNNTTCNNG NNNNNGNNNN NNNNNN
      //
      Primer: PRE_rev
      Region: PRE, insert 3'
      Sequence file: RDB15873_A8Cdd.seq check
      >D05101A5_1_A8Cd_PRE_rev_F03_18_ABI24.ab1
          1 NNNNNNNNNN NNNNNNNNNN NNNCNNCATA GTTAAGANTA CCAGTCAATC TTTCNCAAAT
         61 TTTGTAATCC AGAGGTTGNT TATCGATAAG CTTGATCCCT CGATGTTAAC TCTAGATTAC
        121 TTGTACAGCT CGTCCATGCC GTGAGTGATC CCGGCGGCGG TGCGGAACTC CAGCAGGACC
        181 ATGTGATCGC GCTTCTCGTT GGGGTCTTTG CTCAGGGCGG ACTGGGTGCT CAGGTAGTGG
        241 TTGTCGGGCA GCAGCACGGG GCCGTCGCCG ATGGGGGTGT TCTGCTGGTA GTGGTCGGCG
        301 AGCTGCACGC TGCCGTCCTC GATGTTGTGG CGGATCTTGA AGTTCACCTT GATGCCGTTC
        361 TTCTGCTTGT CGGCCATGAT ATAGATGTTG TGGCTGTTGA AGTTGTACTC CAGCTTGTGC
        421 CCCAGGATGT TGCCGTCCTC CTTGAAGTCG ATGCCCTTCA GCTCGATGCG GTTCACCAGG
        481 GTGTCGCCCT CGAACTTCAC CTCGGCGCGG GTCTTGTAGT TGCCGTCGTC CTTGAAGAAG
        541 ATGGTGCGCT CCTGGACGTA GCCTTCGGGC ATGGCGGACT TGAAGAAGTC GTGCTGCTTC
        601 ATGTGGTCGG GGTAGCGGCT GAAGCACTGC ACGCCGTGGC TCAGGGTGGT CACGAGGGTG
        661 GGCCAGGGCA CGGGCAGCTT GCCGGTGGTG CAGATGAACT TCAGGGTCAG CTTGCCGTAN
        721 GTGGCATCGC CCTCGCCCTC GCCGGACACG CTGAACTTGT GGCCGTTTAC GTCGCCGTCC
        781 AGCTCGACCA GGATGGGCAC CACCCCGGTG NACAGCTCNN CGCNCTNGCT CATGGATNCC
        841 TTANCGCTGG NNTACTNNNN GNTGGNNNTA NNACCCNNNG ANACNGNGNN CTTGGCCNAN
      901 NNCCNNANGN NNCANCNNGN NNNCNNNNNN NNNN
      //
      Primer: HIV-B_R
      Region: HIV-1 psi, 5'LTR
      Sequence file: RDB15873_A8Cde.seq check
      >D05101A5_A8Cd_1_HIV-B_R_E02_14_ABI24.ab1
          1 NNNNNNNNNN NNNNNCNNNN NNANANTTTT GNNGTANTCN CCNGTCGCCG CCCCTCGCCT
         61 CTTGCCGTGC GCGCTTCNGC AAGCCGAGTC CTGCGTCGAG AGAGCTCCTC TGGTTTCCCT
        121 TTCGCTTTCA AGTCCCTGTT CGGGCGCCAC TGCTAGAGAT TTTCCACACT GACTAAAAGG
        181 GTCTGAGGGA TCTCTAGTTA CCAGAGTCAC ACAACAGACG GGCACACACT ACTTGAAGCA
        241 CTCAAGGCAA GCTTTATTGA GGCTTAAGCA GTGGGTTCCC TAGTTAGCCA GAGAGCTCCC
        301 AGGCTCAGAT CTGGTCTAAC CAGAGAGACC CAGTACAGGC AAAACGCGCT GCTTATATAG
        361 ACCTCCCACC GTACACGCCT ACCGCCCATT TGCGTCAATG GGGCGGAGTT GTTACGACAT
        421 TTTGGAAAGT CCCGTTGATT TTGGTGCCAA AACAAACTCC CATTGACGTC AATGGGGTGG
        481 AGACTTGGAA ATCCCCGTGA GTCAAACCGC TATCCACGCC CATTGATGTA CTGCCAAAAC
        541 CGCATCACCA TGGTAATAGC GATGACTAAT ACGTAGATGT ACTGCCAAGT AGGAAAGTCC
        601 CATAAGGTCA TGTACTGGGC ATAATGCCAG GCGGGCCATT TACCGTCATT GACGTCAATA
        661 GGGGGCGTAC TTGGCATATG ATACACTTGA TGTACTGCCA AGTGGGCAGT TTACCGTAAA
        721 TACTCCACCC ATTGACGTCA ATGGAAAGTC CCTATTGGCG TTACTATGGG AACATACGTC
        781 ATTATTGACG TCAATGGGCG GGGGTCGTTG GGCGGTCAGC CAGGCGGGCC ATTTACNGTA
        841 ANTTATGTAA CGCGGANNTC CATATATGGG CTATGAACTA ATGACCCCNN AATTGANNAC
        901 TATTAATANC TANNCNATAA TCAANGTCNA NNNNNATATC NGGNCNGNAC ATCNNNNANN
      961 ANCNNNAANN NNCNNNNNCN NNNNNNNANN NNNNNNNN
      //
      Primer: RRE_check
      Region: RRE
      Sequence file: RDB15873_A8Cdf.seq check
      >D05101A5_A8Cd_1_RRE_check_F02_17_ABI24.ab1
          1 NNNNNNNNNN NNNNNNNNNT TCTTTTCTTG CTGGTTTTGC GATTCTTCAA TTAAGGAGTG
         61 TATTAAGCTT GTGTAATTGT TAATTTCTCT GTCCCACTCC ATCCAGGTCG TGTGATTCCA
        121 AATCTGTTCC AGAGATTTAT TACTCCAACT AGCATTCCAA GGCACAGCAG TGGTGCAAAT
        181 GAGTTTTCCA GAGCAACCCC AAATCCCCAG GAGCTGTTGA TCCTTTAGGT ATCTTTCCAC
        241 AGCCAGGATT CTTGCCTGGA GCTGCTTGAT GCCCCAGACT GTGAGTTGCA ACAGATGCTG
        301 TTGCGCCTCA ATAGCCCTCA GCAAATTGTT CTGCTGCTGC ACTATACCAG ACAATAATTG
        361 TCTGGCCTGT ACCGTCAGCG TCATTGACGC TGCGCCCATA GTGCTTCCTG CTGCTCCCAA
        421 GAACCCAAGG AACAAAGCTC CTATTCCCAC TGCTCTTTTT TCTCTCTGCA CCACTCTTCT
        481 CTTTGCCTTG GTGGGTGCTA CTCCTAATGG TTCAATTTTT ACTACTTTAT ATTTATATAA
        541 TTCACTTCTC CAATTGTCCC TCATATCTCC TCCTCCAGGT CTGAAGATCA GCGGCCGGCC
        601 GCTTGCTGTG CGGTGGTCTT ACTTTTGTTT TGCTCTTCCT CTATCTTGTC TAAAGCTTCC
        661 TTGGTGTCTT TTATCTCTAT CCTTTGATGC ACACAATAGA GGGTTGCTAC TGTATTATAT
        721 AATGATCTAA GTTCTTCTGA TCCTGTCTGA ANGGATGGTT GTAGCTGTCC CAGTATTTGT
        781 CTACAGNCTT CTGATGTTTC TAACANGCCA NGNTTAACTG CNAATCGTTC TANCTCCCTG
        841 CTTGCCCATA CTATATGTTT NAATTNATAT TTTTTTCTTT NCCCCNGGCN NTNANCNNAA
        901 TTTTTTTCCC NTCGCNNTCN NATTCTCCCC CNNCNTANTA NTGNNGCNNN NNNACCNNTN
      961 NNNNNNNNN
      //
      Primer: cPPT+CTS_v2_F
      Region: cPPT_CTS, CMV en_2
      Sequence file: RDB15873_A8Cdg.seq check
      >D05101A5_1_A8Cd_cPPT+CTS_V2_F_E03_15_ABI24.ab1
          1 NNNNNNNNNN NNNNNNNNNN NNTNCNAAAA CNAATTACAA AAATTCAAAA TTTTCGGGTT
         61 TATTACAGGG NCAGNNNNNA TTCACTTTGA ATTAATTCGA TTAATAGTAA TCAATTACGG
        121 GGTCATTAGT TCATAGCCCA TATATGGAGT TCCGCGTTAC ATAACTTACG GTAAATGGCC
        181 CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC AATAATGACG TATGTTCCCA
        241 TAGTAACGCC AATAGGGACT TTCCATTGAC GTCAATGGGT GGAGTANNTN CGGTAAACTG
        301 CCCACTTGGC AGTACATCAA GTGTATCATA TGCCAAGTAC GCCCCCTATT GACGTCAATG
        361 ACGGTAAATG GCCCGCCTGG CATTATGCCC AGTACATGAC CTTATGGGAC TTTCCTACTT
        421 GGCAGTACAT CTACGTATTA GTCATCGCTA TTACCATGGT GATGCGGTTT TGGCAGTACA
        481 TCAATGGGCG TGGATAGCGG TTTGACTCAC GGGGATTTCC AAGTCTCCAC CCCATTGACG
        541 TCAATGGGAG TTTGTTTTGG CACCAAAATC AACGGGACTT TCCAAAATGT CGTAACAACT
        601 CCGCCCCATT GACGCAAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT ATAAGCAGAG
        661 CTGGTTTAGT GAACCGNCNN ATCCGCTAGA GCCACCATGG TAGAGATGCT GCCAACTGTC
        721 GCTGTGCTGG TCTTGGCAGT GTCCGTGGTT GCCAAGGATA ACACCACGCN NCAGGCTAGA
        781 AAGAACGGCA TCAAGGCGAA CTTCACGGNC CGCCACAACN TCNANGANGG CANCNNNCAG
        841 CTCGNCNANN ACTACCAGCA NAACNNNNCC CNTCNNNNAN GGNCCCNNNG CNGCNGCCNN
        901 NNNANCACNA NNTGANNNCC NNNANCGNNN NNNNNCAANN ANCNNNNNNN ANNANNCNNN
      961 NNNNNNN
      //
      Primer: BGH_rev2
      Region: bGH pA, 5'LTR
      Sequence file: RDB15873_A8Cdh.seq check
      >D05101A5_1_A8Cd_BGH_rev2_G03_21_ABI24.ab1
          1 NNNNNNNNNN NNNNNNNNNN NNNGGGGGNN GGGCNAACAA CAGATGGCTG GCAACTAGAA
         61 GGCACAGTCG AGGCTGATCN GCGGGTTTAA ACGGGCCCTG CTAGAGATTT TCCACACTGA
        121 CTAAAAGGGT CTGAGGGATC TCTAGTTACC AGAGTCACAC AACAGACGGG CACACACTAC
        181 TTGAAGCACT CAAGGCAAGC TTTATTGAGG CTTAAGCAGT GGGTTCCCTA GTTAGCCAGA
        241 GAGCTCCCAG GCTCAGATCT GGTCTAACCA GAGAGACCCA GTACAGTCCG GATGCAGCTC
        301 TCGGGCCATG TGATGAAATG CTAGGCGGCT GTCAAACCTC CACTCTAATA CTTCTCTCTC
        361 CGGGTCATCC ATCCCATGCA GGCTCACAGG GTGTAACAAG CGGGTGTTCT CTCCTTCATT
        421 GGCTTCTTCT ACCTTCTCTT GCTCAACTGG TACTAGCTTG TAGCACCATC CAAAGGTCAG
        481 TGGATATCTG ATCCCTGGCC CTGGTGTGTA GTTCTGCCAA TCAGGGAAGT AGCCTTGTGT
        541 GTGGTAGATC CACAGATCAA GGATATCTTG TCTTCGTTGG GAGTGAATTA GCCCTTCCAG
        601 TCCCCCCTTT TCTTTTAAAA AGTGGCTAAG ATCTACAGCT GCCTTGTAAG TCATTGGTCT
        661 TAAAGGTACC TGAGGTGTGA CTGGAAAACC CACCTCCTCC TCCTCTTGTG CTTCTAGCNA
        721 GGCACAATCA GCATTGGTAG CTGCTGTATT GCTACTTGTG ATTGCTCCAT GTTTTTCTAG
        781 GTCTCGACGG TATCGATGCG GGGANGCGGN NNNNANGGAG ATNNGANTCN TCTGANGGCG
        841 AANGCGAAGA NNCGNAANAN GNCGCANANN NNGGCNNCNN NCCNNGGGAA NGNANNNTNC
        901 GCTNNNTTNA GGGNNNNNAN NGNNNNNNNC ANAANNNANN TNNNNNNNNG NNNNCNNGNN
      961 GNNNNNNNNN N
      //
      Primer: SV40pro_F
      Region: EM7 pro, BleoR
      Sequence file: RDB15873_A8Cdi.seq check
      >D05101A5_1_A8Cd_SV40pro_F_H03_24_ABI24.ab1
          1 NNNNNNNNNN NNNNNNNNNN NNNNNANTGN NGNNGCTTTT TTGGANGCCT AGGCTTTTGC
         61 AAAAAGCTCC CGGGAGCTTG TATATCNNTT TTCGGATCTG ATCAGCACGT GTTGACAATT
        121 AATCATCGGC ATAGTATATC GGCATAGTAT AATACGACAA GGTGAGGAAC TAAACCATGG
        181 CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA CCGCGCGCGA CGTCGCCGGA GCGGTCGAGT
        241 TCTGGACCGA CCGGCTCGGG TTCTCCCGGG ACTTCGTGGA GGACGACTTC GCCGGTGTGG
        301 TCCGGGACGA CGTGACCCTG TTCATCAGCG CGGTCCAGGA CCAGGTGGTG CCGGACAACA
        361 CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG ACGAGCTGTA CGCCGAGTGG TCGGAGGTCG
        421 TGTCCACGAA CTTCCGGGAC GCCTCCGGGC CGGCCATGAC CGAGATCGGC GAGCAGCCGT
        481 GGGGGCGGGA GTTCGCCCTG CGCGACCCGG CCGGCAACTG CGTGCACTTC GTGGCCGAGG
        541 AGCAGGACTG ACACGTGCTA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG
        601 GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC
        661 TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA
        721 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT
        781 CCAAACTCAT CAATGTATCT TATCATGTCT GTATACNGTC NANNTCTAGC TAGAGCTNGG
        841 CGTAATCATG GNCATANCTG TTTNCTGNGT GAAANNGTTA NNNGCTCNCN ATNNCNCACA
        901 ACATACGANC NNGANNNNTA AANNGTAAAN NNNNGGGGNN CNNANNNNNN GANNNNNNNN
      961 NN
      //

      Please visit Sequencing and PCR primers for primer information.


      References

      Original, user report and related articles

      original Kinoshita, N., Genetically Encoded Fluorescent Indicator GRAPHIC Delineates Intercellular Connections. iScience 15: 28-38 (2019). PMID 31026667.

      2022.01.16

      GNP_filter3_RDBDEP_html_220112.pl