Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCSII-CMV-MCS _c-GRAPHIC

Lentiviral expression vector of GRAPHIC probe (LZB).

Sequence (full)	RDB15872zzt01.seq compiled by us
Sequence (partial)	RDB15872pka01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Kinoshita, N. et al., iScience 15: 28-38 (2019)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. Additional terms and conditions: The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word] This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
Remarks	Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Kinoshita, N. et al., iScience 15: 28-38 (2019)) を引用し、謝辞の表明を必要とする。本件リソースの使用は学術機関での学術研究に限る。 付加的提供条件： レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします （Schedule AとSchedule Bを入手[link])。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15872_A8Cdp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C-rev Region: insert 5', CMV pro_2 Sequence file: RDB15872_A8Cda.seq
>D05101A4-1_A8Cd_EGFP-C-rev_E01_13_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NCNNNNGNNG GTCTGGGTGC TCNGGTAGTG GTTGTCGGGC    61 AGCAGCACGG GGCCGTCGCC GATGGGGGTG TTCTGCTGGT AGTGGTCGGC GAGCTGCACG   121 CTGCCGTCCT CGACGTTGTG GCGGACCGTG AAGTTCGCCT TGATGCCGTT CTTTCTAGCC   181 TGCAGCGTGG TGTTATCCTT GGCAACCACG GACACTGCCA AGACCAGCAC AGCGACAGTT   241 GGCAGCATCT CTACCATGGT GGCTCTAGCG GATCTGACGG TTCACTAAAC CAGCTCTGCT   301 TATATAGACC TCCCACCGTA CACGCCTACC GCCCATTTGC GTCAATGGGG CGGANTTGTT   361 ACGACATTTT GGAAAGTCCC GTTGATTTTG GTGCCNAAAC AAACTCCCAT TGACGTCAAT   421 GGGGTGGAGA CTTGGAAATC CCCGTGANTC NNACCGCTAT CCACGCCCAT TGATGTACTG   481 CCNAAACCGC ATCACCATGG TAATAGCGAN GACTAATACN TNNATGTACT GNCNAGTNNG   541 AAAGTCCCNT ANNGNCNTGT ACTGGGCNTA NNGNCNNGCG GNNNTTTANC NNCATNNANN   601 TCNNNNNGGG GCGTANTTGG CNNNTGANAC NCTNNNNGNA CNNNNNNNNN GGCANNTNAN 661 NNNAAANNNN NNNNCNNNNG NNNNCNNNGN NAANNNNNNN NNN //
Primer: PRE_rev Region: PRE, insert 3' Sequence file: RDB15872_A8Cdb.seq
>D05101A4_1_A8Cd_PRE_rev_A03_03_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNCNNCA TAGTTAAGAA TACCAGTCAA TCTTTCACAA    61 ATTTTGTAAT CCAGAGGTTG ATTATCGATA AGCTTGATCC CTCGATGTTA ACTCTAGAGG   121 ATCCGCGGCC GCTGCAGAAT TCTCGAGACC GGTAGCGCTA GATTACAGAG AAATGAAGTC   181 CAGGGCTTGG AGGAGGGAGA GGGAAAGCAG CAGCAGCAGC ATCCAGGATG TGTTCTGAAC   241 CAGCAGGCTT ATGCCGCCAT TCTCGAGTTG GTAGCTAGAC GATCCGCCAC CGCCAGAGCC   301 ACCTCCGCCT GAACCGCCTC CACCGCTAGA CGATCCGCCA CCGCCAGAGC CACCTCCGCC   361 TGAACCGCCT CCACCGCTAG ACGATCCGCC ACCGCCAGAG CCACCTCCGC CTGAACCGCC   421 TCCACCGCTA GCCTTCTGGG CCAGCTTCTT CTTCAGGGCC TGGAGCTTCC ACTTGAGCTG   481 GGCGTTCTTC TTCTTCAGGG CTTGCAGCTT CTTCTTGAGC TGGGCCATGG TGGCGCTAGC   541 CGATCCGCCA CCGCCAGAGC CACCTCCGCC TGAACCGCCT CCACCGGATC TCTTGTACAG   601 CTCGTCCATG CCGAGAGTGA TCCCGGCGGC GGTCACGAAC TCCAGCAGGN NCATGTGATC   661 GCGCTTCTCG TTGGGGTCTT TGCTCANGAC GGTCTGGGTG CTCAGGTAGT GNTTGTCGGG   721 CAGCAGCACG GGGCCGTCGC CNATGGGGGT GTTCTGCTGG TAGTGGTCGG CGANCTGCAC   781 GCTGCCGTCC TCNACGTTGT GNCGGANCGN GAAGTTCGCC TTGATGCCGT TCTTTCTANC   841 CNGCANNGNG GNGNTATNCT NNGNNANNNN GGNNNCNGNC NNNANCNGCA CAGNGACNGT 901 NNNNAGCATN NNNACNNNNN NNGNCTNNNN NNNNNN //
Primer: CSII-CMV5' Region: insert mid Sequence file: RDB15872_A8Cdc.seq
>D05101A4_A8Cd_1_CSII-CMV5'_A02_02_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGC TGCCNACTGT CGCTGTGCTG GTCTTGGCAG    61 TGTCCGTGGT TGCCAAGGNT AACACCACGC TGCAGGCTAG AAAGAACGGC ATCAAGGCGA   121 ACTTCACGGT CCGCCACAAC GTCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC   181 AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC   241 AGACCGTCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG   301 TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGAGATCC GGTGGAGGCG   361 GTTCAGGCGG AGGTGGCTCT GGCGGTGGCG GATCGGCTAG CGCCACCATG GCCCAGCTCA   421 AGAAGAAGCT GCAAGCCCTG AAGAAGAAGA ACGCCCAGCT CAAGTGGAAG CTCCAGGCCC   481 TGAAGAAGAA GCTGGCCCAG AAGGCTAGCG GTGGAGGCGG TTCAGGCGGA GGTGGCTCTG   541 GCGGTGGCGG ATCGTCTAGC GGTGGAGGCG GTTCAGGCGG AGGTGGCTCT GGCGGTGGCG   601 GATCGTCTAG CGGTGGAGGC GGTTCAGGCG GAGGTGGCTC TGGCGGTGGC GGATCGTCTA   661 GCTACCAACT CGAGAATGGC GGCATAAGCC TGCTGGTTCA GAACACATCC TGGATGCTGC   721 TGCTGCTGCT TTCCCTCTCC CTCCTCCAAG CCCTGGACTT CATTTCTCTG TAATCTAGCG   781 CTACCGGTCT CGAGAATTCT GCAGCGGCCG CGGNTCCTCT AGAGTTAACA TCGANGGATC   841 AAGCTTATCG ATAATCAACC TCTGGANTAC AAAATTTGTG AAAGANTGAC TNNNATTCTT   901 AACTATGTNG CTNCTTTTAC GCTATGNNNA TANNCTGCNT TNANGNNNTT GNATCANGNT 961 ATNNNNTNNC CNNTATGNNT TTNNATTTTT NNNNNNNNNN //
Primer: HIV-B_R Region: HIV-1 psi, 5'LTR, CMV pro_1, CMV en_1 Sequence file: RDB15872_A8Cdd.seq
>D05101A4_A8Cd_1_HIV-B_R_G01_19_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTTT TGGCGNANTC NCCNGTCGCC GCCCCTCGCC    61 TCTTGCCGTG CGCGCTTCAG CAAGCCGAGT CCTGCGTCGA GAGAGCTCCT CTGGTTTCCC   121 TTTCGCTTTC AAGTCCCTGT TCGGGCGCCA CTGCTAGAGA TTTTCCACAC TGACTAAAAG   181 GGTCTGAGGG ATCTCTAGTT ACCAGAGTCA CACAACAGAC GGGCACACAC TACTTGAAGC   241 ACTCAAGGCA AGCTTTATTG AGGCTTAAGC AGTGGGTTCC CTAGTTAGCC AGAGAGCTCC   301 CAGGCTCAGA TCTGGTCTAA CCAGAGAGAC CCAGTACAGG CAAAACGCGC TGCTTATATA   361 GACCTCCCAC CGTACACGCC TACCGCCCAT TTGCGTCAAT GGGGCGGAGT TGTTACGACA   421 TTTTGGAAAG TCCCGTTGAT TTTGGTGCCA AAACAAACTC CCATTGACGT CAATGGGGTG   481 GAGACTTGGA AATCCCCGTG AGTCAAACCG CTATCCACGC CCATTGATGT ACTGCCAAAA   541 CCGCATCACC ATGGTAATAG CGATGACTAA TACGTAGATG TACTGCCAAG TAGGAAAGTC   601 CCATAAGGTC ATGTACTGGG CATAATGCCA GGCGGGCCAT TTACCGTCAT TGACGTCAAT   661 AGGGGGCGTA CTTGGCATAT GATACACTTG ATGTACTGCC AAGTGGGCAG TTTACCGTAA   721 ATACTCCACC CATTGACGTN NATGGAAAGT CCCTATTGNN GTTACTATGG GAACATACGT   781 CATTATTGAC GTCNATGGGC GGGGGTCGTT GGGCGGTCAG NNAGGCGGGC CATTTACCGT   841 AAGTTATGTA ACGCGNANCT CCNTATATGG GNCTATGNAN TAANGACCCC NTAATNNATN 901 ACTANNAANN ACNNNNCAAT AATCNNNNNN NNNN //
Primer: BGH_rev2 Region: 3'LTR, PRE Sequence file: RDB15872_A8Cde.seq
>D05101A4_A8Cd_1_BGH_rev2_A01_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN GGGGGNNGGG CANNNACAGN NGGCTGGCAA CTAGAAGGCA    61 CAGTCGAGGC TGATCAGCGG GTTTAAACGG GCCCTGCTAG AGATTTTCCA CACTGACTAA   121 AAGGGTCTGA GGGATCTCTA GTTACCAGAG TCACACAACA GACGGGCACA CACTACTTGA   181 AGCACTCAAG GCAAGCTTTA TTGAGGCTTA AGCAGTGGGT TCCCTAGTTA GCCAGAGAGC   241 TCCCAGGCTC AGATCTGGTC TAACCAGAGA GACCCAGTAC AGTCCGGATG CAGCTCTCGG   301 GCCATGTGAT GAAATGCTAG GCGGCTGTCA AACCTCCACT CTAATACTTC TCTCTCCGGG   361 TCATCCATCC CATGCAGGCT CACAGGGTGT AACAAGCGGG TGTTCTCTCC TTCATTGGCT   421 TCTTCTACCT TCTCTTGCTC AACTGGTACT AGCTTGTAGC ACCATCCAAA GGTCAGTGGA   481 TATCTGATCC CTGGCCCTGG TGTGTAGTTC TGCCAATCAG GGAAGTAGCC TTGTGTGTGG   541 TAGATCCACA GATCAAGGAT ATCTTGTCTT CGTTGGGAGT GAATTAGCCC TTCCAGTCCC   601 CCCTTTTCTT TTAAAAAGTG GCTAAGATCT ACAGCTGCCT TGTAAGTCAT TGGTCTTAAA   661 GGTACCTGAG GTGTGACTGG AAAACCCACC TCCTCCTCCT CTTGTGCTTC TAGCCAGGCA   721 CAATCAGCAT TGGTAGCTGC TGTATTGCTA CTTGTGATTG CTCCATGTTT TTCTAGGTCT   781 CGACGGTATC GATGCGGGGA NGCGGCCCAA AGGGAGATCC GACTCGTCTG ANGGCGAANG   841 CGAANANNCG GAANANGNCG CAGANCNNGC AGCANNNCGC GGGAAGGAAG GTCCNCTGGA   901 TNNAGGGNNG AAGGGNNGTA NCANANNNAC NNCCCGNGNN NAANCCAGGN GGNNNCNCNN 961 GNNANCAGNC NNNNGNNANN GNNNNNNNNN TNNNNNNNNN N //
Primer: SV40pro_F Region: BleoR, SV40 pA Sequence file: RDB15872_A8Cdf.seq
>D05101A4_1_A8Cd_SV40pro_F_C03_09_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNGTGNNG NNGCTTTTTT GGNNGCCTAN GCTTTTGCAA    61 AAAGCTCCCG GGAGCTTGNA TATCNNTTTT CGGATCTGAT CAGCACGTGT TGACAATTAA   121 TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG TGAGGAACTA AACCATGGCC   181 AAGTTGACCA GTGCCGTTCC GGNNNNNNNC GCGCGCGACG TCGCCGGAGC GGTCGAGTTC   241 TGGACCGACC GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG ACGACTTCGC CGGTGTGGTC   301 CGGGACGACG TGACCCTGTT CATCAGCGCG GTCCAGGACC AGGTGGTGCC GGACAACACC   361 CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC GAGCTGTACG CCGAGTGGTC GGAGGTCGTG   421 TCCACGAACT TCCGGGACGC CTCCGGGCCG GCCATGACCG AGATCGGCGA GCAGCCGTGG   481 GGGCGGGAGT TCGCCCTGCG CGACCCGGCC GGCAACTGCG TGCACTTCGT GGCCGAGGAG   541 CAGGACTGAC ACGTGCTACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC   601 TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG   661 GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT   721 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCN   781 NAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCNA CCTCTAGCTA GAGCTTGGCG   841 TANTCATGGN CATANCTGTT NCCTGTGTGA AATTGTTATN CGNNCACAAT TNCACACNAC   901 ATACNNNCNG GNNNCATAAA GNGNNAAGNN NNGGGNNNNN NNNGANNNNN CNNNNNNNNN 961 N //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.10.12

GNP_filter3_RDBDEP_html_221010.pl