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pCSII-CMV-MCS _c-GRAPHIC

Lentiviral expression vector of GRAPHIC probe (LZB).

Catalog number RDB15872
Resource name pCSII-CMV-MCS _c-GRAPHIC
Clone info. Lentiviral expression vector of GRAPHIC probe (GPI anchored Reconstitution-Activated Proteins Highlight Intercellular Connections), fluorescent indicator for intercellular contacts with optimized intercellular GFP reconstitution. This probe contains an mouse preproacrosin signal peptide (SP), superfolder GFP-CT, basic leucine zipper domain (LZB), mouse Thy-1 GPI-anchored domain, see Fig. 1 of Kinoshita, N. et al., iScience 15: 28-38, 2019.
Expression was confirmed by the depositor with detection of reconstituted GFP fluorescence. Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-CMV-MCS (plasmid)
Size of vector backbone 8.6 kb
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Gene/insert name mouse Acr cDNA mouse Thy1 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Shimogori, Tomomi | Kinoshita, Nagatoki |
Other clones in our bank

External Database
mouse Acr mouse Thy1
    Aequorea victoria GFP

        Reference sequence
          

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Kinoshita, N. et al., iScience 15: 28-38 (2019)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
        Additional terms and conditions for distribution The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
        Ordering Please visit Information of Request for Distribution.[link] 
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        Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
        Schedule A (GFP-A) [Link]
        Remarks Remember that you will be working with samples containing infectious virus.
        Please use Schedule A (GFP-A) additionally.
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        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB15872 pCSII-CMV-MCS _c-GRAPHIC DNA solution

        Ordering Information [in Japanese] [in English]
        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Electronic file

        Sequence (full) RDB15872zzt01.seq compiled by us
        Sequence (partial) RDB15872pka01.seq provided by the depositor

        Original reference

        original Kinoshita, N., Genetically Encoded Fluorescent Indicator GRAPHIC Delineates Intercellular Connections. iScience 15: 28-38 (2019). PMID 31026667.

        Further references such as user reports and related articles (go to bottom)

        Featured content


        Sequence information

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB15872_A8Cdp1-1.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: EGFP-C-rev
        Region: insert 5', CMV pro_2
        Sequence file: RDB15872_A8Cda.seq check
        >D05101A4-1_A8Cd_EGFP-C-rev_E01_13_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NCNNNNGNNG GTCTGGGTGC TCNGGTAGTG GTTGTCGGGC
           61 AGCAGCACGG GGCCGTCGCC GATGGGGGTG TTCTGCTGGT AGTGGTCGGC GAGCTGCACG
          121 CTGCCGTCCT CGACGTTGTG GCGGACCGTG AAGTTCGCCT TGATGCCGTT CTTTCTAGCC
          181 TGCAGCGTGG TGTTATCCTT GGCAACCACG GACACTGCCA AGACCAGCAC AGCGACAGTT
          241 GGCAGCATCT CTACCATGGT GGCTCTAGCG GATCTGACGG TTCACTAAAC CAGCTCTGCT
          301 TATATAGACC TCCCACCGTA CACGCCTACC GCCCATTTGC GTCAATGGGG CGGANTTGTT
          361 ACGACATTTT GGAAAGTCCC GTTGATTTTG GTGCCNAAAC AAACTCCCAT TGACGTCAAT
          421 GGGGTGGAGA CTTGGAAATC CCCGTGANTC NNACCGCTAT CCACGCCCAT TGATGTACTG
          481 CCNAAACCGC ATCACCATGG TAATAGCGAN GACTAATACN TNNATGTACT GNCNAGTNNG
          541 AAAGTCCCNT ANNGNCNTGT ACTGGGCNTA NNGNCNNGCG GNNNTTTANC NNCATNNANN
          601 TCNNNNNGGG GCGTANTTGG CNNNTGANAC NCTNNNNGNA CNNNNNNNNN GGCANNTNAN
        661 NNNAAANNNN NNNNCNNNNG NNNNCNNNGN NAANNNNNNN NNN
        //
        Primer: PRE_rev
        Region: PRE, insert 3'
        Sequence file: RDB15872_A8Cdb.seq check
        >D05101A4_1_A8Cd_PRE_rev_A03_03_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNCNNCA TAGTTAAGAA TACCAGTCAA TCTTTCACAA
           61 ATTTTGTAAT CCAGAGGTTG ATTATCGATA AGCTTGATCC CTCGATGTTA ACTCTAGAGG
          121 ATCCGCGGCC GCTGCAGAAT TCTCGAGACC GGTAGCGCTA GATTACAGAG AAATGAAGTC
          181 CAGGGCTTGG AGGAGGGAGA GGGAAAGCAG CAGCAGCAGC ATCCAGGATG TGTTCTGAAC
          241 CAGCAGGCTT ATGCCGCCAT TCTCGAGTTG GTAGCTAGAC GATCCGCCAC CGCCAGAGCC
          301 ACCTCCGCCT GAACCGCCTC CACCGCTAGA CGATCCGCCA CCGCCAGAGC CACCTCCGCC
          361 TGAACCGCCT CCACCGCTAG ACGATCCGCC ACCGCCAGAG CCACCTCCGC CTGAACCGCC
          421 TCCACCGCTA GCCTTCTGGG CCAGCTTCTT CTTCAGGGCC TGGAGCTTCC ACTTGAGCTG
          481 GGCGTTCTTC TTCTTCAGGG CTTGCAGCTT CTTCTTGAGC TGGGCCATGG TGGCGCTAGC
          541 CGATCCGCCA CCGCCAGAGC CACCTCCGCC TGAACCGCCT CCACCGGATC TCTTGTACAG
          601 CTCGTCCATG CCGAGAGTGA TCCCGGCGGC GGTCACGAAC TCCAGCAGGN NCATGTGATC
          661 GCGCTTCTCG TTGGGGTCTT TGCTCANGAC GGTCTGGGTG CTCAGGTAGT GNTTGTCGGG
          721 CAGCAGCACG GGGCCGTCGC CNATGGGGGT GTTCTGCTGG TAGTGGTCGG CGANCTGCAC
          781 GCTGCCGTCC TCNACGTTGT GNCGGANCGN GAAGTTCGCC TTGATGCCGT TCTTTCTANC
          841 CNGCANNGNG GNGNTATNCT NNGNNANNNN GGNNNCNGNC NNNANCNGCA CAGNGACNGT
        901 NNNNAGCATN NNNACNNNNN NNGNCTNNNN NNNNNN
        //
        Primer: CSII-CMV5'
        Region: insert mid
        Sequence file: RDB15872_A8Cdc.seq check
        >D05101A4_A8Cd_1_CSII-CMV5'_A02_02_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGC TGCCNACTGT CGCTGTGCTG GTCTTGGCAG
           61 TGTCCGTGGT TGCCAAGGNT AACACCACGC TGCAGGCTAG AAAGAACGGC ATCAAGGCGA
          121 ACTTCACGGT CCGCCACAAC GTCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC
          181 AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC
          241 AGACCGTCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG
          301 TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGAGATCC GGTGGAGGCG
          361 GTTCAGGCGG AGGTGGCTCT GGCGGTGGCG GATCGGCTAG CGCCACCATG GCCCAGCTCA
          421 AGAAGAAGCT GCAAGCCCTG AAGAAGAAGA ACGCCCAGCT CAAGTGGAAG CTCCAGGCCC
          481 TGAAGAAGAA GCTGGCCCAG AAGGCTAGCG GTGGAGGCGG TTCAGGCGGA GGTGGCTCTG
          541 GCGGTGGCGG ATCGTCTAGC GGTGGAGGCG GTTCAGGCGG AGGTGGCTCT GGCGGTGGCG
          601 GATCGTCTAG CGGTGGAGGC GGTTCAGGCG GAGGTGGCTC TGGCGGTGGC GGATCGTCTA
          661 GCTACCAACT CGAGAATGGC GGCATAAGCC TGCTGGTTCA GAACACATCC TGGATGCTGC
          721 TGCTGCTGCT TTCCCTCTCC CTCCTCCAAG CCCTGGACTT CATTTCTCTG TAATCTAGCG
          781 CTACCGGTCT CGAGAATTCT GCAGCGGCCG CGGNTCCTCT AGAGTTAACA TCGANGGATC
          841 AAGCTTATCG ATAATCAACC TCTGGANTAC AAAATTTGTG AAAGANTGAC TNNNATTCTT
          901 AACTATGTNG CTNCTTTTAC GCTATGNNNA TANNCTGCNT TNANGNNNTT GNATCANGNT
        961 ATNNNNTNNC CNNTATGNNT TTNNATTTTT NNNNNNNNNN
        //
        Primer: HIV-B_R
        Region: HIV-1 psi, 5'LTR, CMV pro_1, CMV en_1
        Sequence file: RDB15872_A8Cdd.seq check
        >D05101A4_A8Cd_1_HIV-B_R_G01_19_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTTT TGGCGNANTC NCCNGTCGCC GCCCCTCGCC
           61 TCTTGCCGTG CGCGCTTCAG CAAGCCGAGT CCTGCGTCGA GAGAGCTCCT CTGGTTTCCC
          121 TTTCGCTTTC AAGTCCCTGT TCGGGCGCCA CTGCTAGAGA TTTTCCACAC TGACTAAAAG
          181 GGTCTGAGGG ATCTCTAGTT ACCAGAGTCA CACAACAGAC GGGCACACAC TACTTGAAGC
          241 ACTCAAGGCA AGCTTTATTG AGGCTTAAGC AGTGGGTTCC CTAGTTAGCC AGAGAGCTCC
          301 CAGGCTCAGA TCTGGTCTAA CCAGAGAGAC CCAGTACAGG CAAAACGCGC TGCTTATATA
          361 GACCTCCCAC CGTACACGCC TACCGCCCAT TTGCGTCAAT GGGGCGGAGT TGTTACGACA
          421 TTTTGGAAAG TCCCGTTGAT TTTGGTGCCA AAACAAACTC CCATTGACGT CAATGGGGTG
          481 GAGACTTGGA AATCCCCGTG AGTCAAACCG CTATCCACGC CCATTGATGT ACTGCCAAAA
          541 CCGCATCACC ATGGTAATAG CGATGACTAA TACGTAGATG TACTGCCAAG TAGGAAAGTC
          601 CCATAAGGTC ATGTACTGGG CATAATGCCA GGCGGGCCAT TTACCGTCAT TGACGTCAAT
          661 AGGGGGCGTA CTTGGCATAT GATACACTTG ATGTACTGCC AAGTGGGCAG TTTACCGTAA
          721 ATACTCCACC CATTGACGTN NATGGAAAGT CCCTATTGNN GTTACTATGG GAACATACGT
          781 CATTATTGAC GTCNATGGGC GGGGGTCGTT GGGCGGTCAG NNAGGCGGGC CATTTACCGT
          841 AAGTTATGTA ACGCGNANCT CCNTATATGG GNCTATGNAN TAANGACCCC NTAATNNATN
        901 ACTANNAANN ACNNNNCAAT AATCNNNNNN NNNN
        //
        Primer: BGH_rev2
        Region: 3'LTR, PRE
        Sequence file: RDB15872_A8Cde.seq check
        >D05101A4_A8Cd_1_BGH_rev2_A01_01_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNNN GGGGGNNGGG CANNNACAGN NGGCTGGCAA CTAGAAGGCA
           61 CAGTCGAGGC TGATCAGCGG GTTTAAACGG GCCCTGCTAG AGATTTTCCA CACTGACTAA
          121 AAGGGTCTGA GGGATCTCTA GTTACCAGAG TCACACAACA GACGGGCACA CACTACTTGA
          181 AGCACTCAAG GCAAGCTTTA TTGAGGCTTA AGCAGTGGGT TCCCTAGTTA GCCAGAGAGC
          241 TCCCAGGCTC AGATCTGGTC TAACCAGAGA GACCCAGTAC AGTCCGGATG CAGCTCTCGG
          301 GCCATGTGAT GAAATGCTAG GCGGCTGTCA AACCTCCACT CTAATACTTC TCTCTCCGGG
          361 TCATCCATCC CATGCAGGCT CACAGGGTGT AACAAGCGGG TGTTCTCTCC TTCATTGGCT
          421 TCTTCTACCT TCTCTTGCTC AACTGGTACT AGCTTGTAGC ACCATCCAAA GGTCAGTGGA
          481 TATCTGATCC CTGGCCCTGG TGTGTAGTTC TGCCAATCAG GGAAGTAGCC TTGTGTGTGG
          541 TAGATCCACA GATCAAGGAT ATCTTGTCTT CGTTGGGAGT GAATTAGCCC TTCCAGTCCC
          601 CCCTTTTCTT TTAAAAAGTG GCTAAGATCT ACAGCTGCCT TGTAAGTCAT TGGTCTTAAA
          661 GGTACCTGAG GTGTGACTGG AAAACCCACC TCCTCCTCCT CTTGTGCTTC TAGCCAGGCA
          721 CAATCAGCAT TGGTAGCTGC TGTATTGCTA CTTGTGATTG CTCCATGTTT TTCTAGGTCT
          781 CGACGGTATC GATGCGGGGA NGCGGCCCAA AGGGAGATCC GACTCGTCTG ANGGCGAANG
          841 CGAANANNCG GAANANGNCG CAGANCNNGC AGCANNNCGC GGGAAGGAAG GTCCNCTGGA
          901 TNNAGGGNNG AAGGGNNGTA NCANANNNAC NNCCCGNGNN NAANCCAGGN GGNNNCNCNN
        961 GNNANCAGNC NNNNGNNANN GNNNNNNNNN TNNNNNNNNN N
        //
        Primer: SV40pro_F
        Region: BleoR, SV40 pA
        Sequence file: RDB15872_A8Cdf.seq check
        >D05101A4_1_A8Cd_SV40pro_F_C03_09_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNGTGNNG NNGCTTTTTT GGNNGCCTAN GCTTTTGCAA
           61 AAAGCTCCCG GGAGCTTGNA TATCNNTTTT CGGATCTGAT CAGCACGTGT TGACAATTAA
          121 TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG TGAGGAACTA AACCATGGCC
          181 AAGTTGACCA GTGCCGTTCC GGNNNNNNNC GCGCGCGACG TCGCCGGAGC GGTCGAGTTC
          241 TGGACCGACC GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG ACGACTTCGC CGGTGTGGTC
          301 CGGGACGACG TGACCCTGTT CATCAGCGCG GTCCAGGACC AGGTGGTGCC GGACAACACC
          361 CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC GAGCTGTACG CCGAGTGGTC GGAGGTCGTG
          421 TCCACGAACT TCCGGGACGC CTCCGGGCCG GCCATGACCG AGATCGGCGA GCAGCCGTGG
          481 GGGCGGGAGT TCGCCCTGCG CGACCCGGCC GGCAACTGCG TGCACTTCGT GGCCGAGGAG
          541 CAGGACTGAC ACGTGCTACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC
          601 TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG
          661 GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT
          721 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCN
          781 NAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCNA CCTCTAGCTA GAGCTTGGCG
          841 TANTCATGGN CATANCTGTT NCCTGTGTGA AATTGTTATN CGNNCACAAT TNCACACNAC
          901 ATACNNNCNG GNNNCATAAA GNGNNAAGNN NNGGGNNNNN NNNGANNNNN CNNNNNNNNN
        961 N
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Kinoshita, N., Genetically Encoded Fluorescent Indicator GRAPHIC Delineates Intercellular Connections. iScience 15: 28-38 (2019). PMID 31026667.

        2021.11.25

        GNP_filter3_RDBDEP_html_211111.pl