Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCSII-CMV-MCS_B (BFP type) n-GRAPHIC T2A H2B-mCherry (#RDB15871)

Lentiviral expression vector of GRAPHIC probe (LZA).

Clone info. Lentiviral expression vector of GRAPHIC probe (GPI anchored Reconstitution-Activated Proteins Highlight Intercellular Connections), fluorescent indicator for intercellular contacts with optimized intercellular GFP reconstitution. This probe contains an mouse preproacrosin signal peptide (SP), superfolder GFP-NT (BFP type), acidic leucine zipper domain (LZA), mouse Thy-1 GPI-anchored domain, 2A peptide, human histone 2B (H2B), mCherry, see Fig. 1 of Kinoshita, N. et al., iScience 15: 28-38, 2019.
Comment Expression was confirmed by the depositor with detection of reconstituted BFP fluorescence and nucleic mCherry fluorecence.
Vector backbone CSII-CMV-MCS (RDB04377) (plasmid)
Size of vector backbone 8.6 kb
Selectable markers Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Gene/insert name mouse Acr cDNA mouse Thy1 cDNA human histon H2BJ cDNA Aequorea victoria GFP cDNA Discosoma sp. RFP cDNA
Depositor|Developer Shimogori, Tomomi | Kinoshita, Nagatoki |
 
Sequence (full) RDB15871zzt01.seq compiled by us
Sequence (partial) RDB15871pka01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose)[Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Kinoshita, N. et al., iScience 15: 28-38 (2019)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
Additional terms and conditions:
The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Remarks Remember that you will be working with samples containing infectious virus.
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利機関による非営利学術研究用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Kinoshita, N. et al., iScience 15: 28-38 (2019)) を引用し、謝辞の表明を必要とする。本件リソースの使用は学術機関での学術研究に限る。
MTAに書く付加的使用条件:
レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15871 pCSII-CMV-MCS_B (BFP type) n-GRAPHIC T2A H2B-mCherry DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCSII-CMV-MCS_B (BFP type) n-GRAPHIC T2A H2B-mCherry was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15871).

Reference section:

Kinoshita, N., Huang, A.J.Y., McHugh, T.J., Suzuki, S.C., Masai, I., Kim, I.H., Soderling, S.H., Miyawaki, A., Shimogori, T., Genetically Encoded Fluorescent Indicator GRAPHIC Delineates Intercellular Connections. iScience 15: 28-38 (2019). PMID 31026667. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15871_A8Cdp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: YFP_R (Pr0127)
Region: insert 5', CMV pro_2, CMV en_2
Sequence file: RDB15871_A8Cda.seq check
>D05101A3_A8Cd_2_YFP_R_A01_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNCCN NGNNNGCNNN NTTGTGGCCG TTTACGTCGC CGTCCNGCTC
   61 GACCAGGATG GGCACCACCC CGGTGAACAG CTCCTCGCCC TTGCTCACTC TAGCCTGCAG
  121 CGTGGTGTTA TCCTTGGCAA CCACGGACAC TGCCAAGACC AGCACAGCGA CAGTTGGCAG
  181 CATCTCTACC ATGGTGGCTC TAGCGGATCT GACGGTTCAC TAAACCAGCT CTGCTTATAT
  241 AGACCTCCCA CCGTACACGC CTACCGCCCA TTTGCGTCAA TGGGGCGGAG TTGTTACGAC
  301 ATTTTGGAAA GTCCCGTTGA TTTTGGTGCC AAAACAAACT CCCATTGACG TCAATGGGGT
  361 GGAGACTTGG AAATCCCCGT GAGTCAAACC GCTATCCACG CCCATTGATG TACTGCCAAA
  421 ACCGCATCAC CATGGTAATA GCGATGACTA ATACGTAGAT GTACTGCCAA GTAGGAAAGT
  481 CCCATAAGGT CATGTACTGG GCATAATGCC AGGCGGGCCA TTTACCGTCA TTGACGTCAA
  541 TAGGGGGCGT ACTTGGCATA TGATACACTT GATGTACTGC CAAGTGGGCA GTTTACCGTA
  601 AATACTCCAC CCATTGACGT CAATGGAAAG TCCCTATTGG CGTTACTATG GGAACATACG
  661 TCATTATTGA CGTCAATGGG CGGGGGTCGT TGGGCGGTCA GCCAGGCGGG CCATTTACCG
  721 TAAGTTATGT AACGCGGANC TCCATATATG GGCTATGAAC TAATGNNCCC NTAATTGATT
  781 ACTATTAATC GAATTAATTC NNGTGAATTT CTGCTGTCCC TGTAATAAAC CNNAAAATTT
  841 TGAATTTTTG TAATTTGTTT TTGTAANTCT TTANTTTGTA TGTCNGTTGC TANTATGTCN
  901 ACNANTCTTT CCCCNGCNCT NNACCCCCNN NNNNCCCNCN NTTTNNTTNA AAANTNGNNG
961 NNNNNNNNNN
//
Primer: PRE_rev (Pr0250)
Region: PRE, insert 3'
Sequence file: RDB15871_A8Cdb.seq check
>D05101A3_2_A8Cd_PRE_rev_D02_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNCNNN ATAGTTAAGA ATACCAGTCA ATCTTTCNCA
   61 AATTTTGTAA TCCAGAGGTT GNTTATCGAT AAGCTTGATC CCTCGATGTT AACTCTAGAT
  121 TACTTGTACA GCTCGTCCAT GCCGCCGGTG GAGTGGCGGC CCTCGGCGCG TTCGTACTGT
  181 TCCACGATGG TGTAGTCCTC GTTGTGGGAG GTGATGTCCA ACTTGATGTT GACGTTGTAG
  241 GCGCCGGGCA GCTGCACGGG CTTCTTGGCC TTGTAGGTGG TCTTGACCTC AGCGTCGTAG
  301 TGGCCGCCGT CCTTCAGCTT CAGCCTCTGC TTGATCTCGC CCTTCAGGGC GCCGTCCTCG
  361 GGGTACATCC GCTCGGAGGA GGCCTCCCAG CCCATGGTCT TCTTCTGCAT TACGGGGCCG
  421 TCGGAGGGGA AGTTGGTGCC GCGCAGCTTC ACCTTGTAGA TGAACTCGCC GTCCTGCAGG
  481 GAGGAGTCCT GGGTCACGGT CACCACGCCG CCGTCCTCGA AGTTCATCAC GCGCTCCCAC
  541 TTGAAGCCCT CGGGGAAGGA CAGCTTCAAG TAGTCGGGGA TGTCGGCGGG GTGCTTCACG
  601 TAGGCCTTGG AGCCGTACAT GAACTGAGGG GACAGGATGT CCCAGGCGAA NGGCAGGGGG
  661 CCACCCTTGG TCACCTTCAG CTTGGCGGTC TGGGTGCCCT CGTAGGGGCG GCCCTCGCCC
  721 TCGCCCTCGA TCTCGAACTC GTGGCCGTTC ACGGANCCCT CCATGTGCAC CTTGAAGCGC
  781 ATGAACTCCT TGATGATGGC CATGTTATCC TCCTCGCCNN TGCTCACCAT GGNGNCTGNN
  841 TCCTTANCGC TGGTGTACTT GGNGATNGNC TTANNANCNN CGGANACGGN GNGCTNNNNN
901 NNNTCNNNNN GNANCAGCNN GNNNNCNNNN NNNNTGGNTN NNCNNNNNNN NNN
//
Primer: CSII-CMV5' (Pr0021)
Region: insert mid
Sequence file: RDB15871_A8Cdc.seq check
>D05101A3_A8Cd_2_CSII-CMV5'_F01_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNTGTCNNNN NNCNNNGNCT
   61 TNNNNNNNGN NCGTGGTTGC CNNNGNTAAC ACCACGCTGC AGGCTAGAGT GAGCAAGGGC
  121 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC
  181 CACAAGTTCA GCGTGCGCGG CGAGGGCGAG GGCGATGCCA CCAACGGCAA GCTGACCCTG
  241 AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG
  301 AGCCACGGCG TGCAGTGCTT CGCCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC
  361 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTCCTTCAA GGACGACGGC
  421 ACCTACAAGA CCCGCGCCGT TGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG
  481 CTGAAGGGCG TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGNNNNNN
  541 TTCAACAGCC ACNNCATCTA TATCACGGCC GTCAAGCAGG CTAGAGGTGG AGGCGGTTCA
  601 GGCGGAGGTG GCTCTGGCGG TGGCGGATCG GCTAGCGCCA CCATGGCCCA GCTCGAAAAG
  661 GANCTGCAAG CCCTGGNAGA ANGAGAANGC CCAGCTCGAA TGGGAGCTCC ANGCCCTGGA
  721 GAANGAGCTG GNCCNNAANG CTAGCGGTGG AGGCNNNTCA GGCGGANGTG GCTCTGGCGG
  781 NNGCGGATCG TCTANCGGNG GANGCGNNTC ANGCGGAGGT GGNTCTGGCG GTGGCGNNTC
  841 NNCTNNCGGT GGANGNNGNN TNCAGGCGGA NGTGGCTCTG GNGGNNGNNG GATCGTCTAG
  901 CTACCNAANT CGAGAATGGC NGCNTANNCC NTGCTGGNTT CNNAANNNNT NCNNGNNNNG
  961 CNNNCNNGCT GCTGNNNTTT CCNNNNNCCC NNNNNNNCNA NGCCCNTNNA CNTNNATTTN
1021 NNNNNNNCNN NNNNNNNGGN NNNNANNNNN NNNNN
//
Primer: VN_F1 (Pr0992)
Region: insert mid
Sequence file: RDB15871_A8Cdd.seq check
>D05101A3_A8Cd_2_VN_F1_A01_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NTCTATATNN CGGCCGNCNN NNANNGCTAG
   61 AGGTGGAGGC GGTTCAGGCG GAGGTGGCTC TGGCGGTGGC GGATCGGCTA GCGCCACCAT
  121 GGCCCAGCTC GAAAAGGAGC TGCAAGCCCT GGAGAAGGAG AACGCCCAGC TCGAATGGGA
  181 GCTCCAGGCC CTGGAGAAGG AGCTGGCCCA GAAGGCTAGC GGTGGAGGCG GTTCAGGCGG
  241 AGGTGGCTCT GGCGGTGGCG GATCGTCTAG CGGTGGAGGC GGTTCAGGCG GAGGTGGCTC
  301 TGGCGGTGGC GGATCGTCTA GCGGTGGAGG CGGTTCAGGC GGAGGTGGCT CTGGCGGTGG
  361 CGGATCGTCT AGCTACCAAC TCGAGAATGG CGGCATAAGC CTGCTGGTTC AGAACACATC
  421 CTGGATGCTG CTGCTGCTGC TTTCCCTCTC CCTCCTCCAA GCCCTGGACT TCATTTCTCT
  481 GTCTAGCGAG GGCAGAGGAA GTCTTCTAAC ATGCGGTGAC GTCGAGGAGA ATCCTGGCCC
  541 AATGCCAGAG CCAGCGAAGT CTGCTCCCGC CCCGAAAAAG GGCTCCAAGA AGGCGGTGAC
  601 TAAGGCGCAN AAGAAAGGCG GCAAGAAGCG CAAGCGCAGC CGCAAGGAGA GCTATTCCAT
  661 CTATGTGTAC AAGGTTCTGA AGCAGGTCCA CCCTGACACC GGCATTTCGT CCAAGGCCAT
  721 GGGCATCATG AATTCGTTTG TGAACGACAT TTTCGAGCGC ATCGCAGGTG AGGCTTCCCG
  781 CCTGGCGCAT TACANCAAGC GCTCGACCAT CACCTCNAGG GAGATCCAGA NNGNCGTGCG
  841 CCTGCTGCTG NCNGGGGAGT TGGNCNAGCA CGCCGTGTCC GANGGTACTA AGGCCATCNN
  901 NNAGTACACC AGCGCNNANG ANCCNGCCAC CNTNNTGANN ANGGGCGNNG ANGANAACAT
  961 GGNCNTCATN NANNNANTTC NTGCGCTTCN NNGNGNNCAT GNNNGGCTNC NNNNNNNGGC
 1021 CNCNANTNCN AGATNNAGGN NNANNNNNAN GNNNNNNCNT NNNNNNGNNN NCNNNNNNNN
1081 NN
//
Primer: mRFP-M_R (Pr0674)
Region: insert mid
Sequence file: RDB15871_A8Cde.seq check
>D05101A3_A8Cd_2_mRFP-M_R_B01_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GGNNCCGNNN NNGAACTGAG GGGANAGGNN
   61 GTCCCAGGCG AAGGGCAGGG GGCCACCCTT GGTCACCTTC AGCTTGGCGG TCTGGGTGCC
  121 CTCGTAGGGG CGGCCCTCGC CCTCGCCCTC GATCTCGAAC TCGTGGCCGT TCACGGAGCC
  181 CTCCATGTGC ACCTTGAAGC GCATGAACTC CTTGATGATG GCCATGTTAT CCTCCTCGCC
  241 CTTGCTCACC ATGGTGGCTG GATCCTTAGC GCTGGTGTAC TTGGTGATGG CCTTAGTACC
  301 CTCGGACACG GCGTGCTTGG CCAACTCCCC AGGCAGCAGC AGGCGCACGG CCGTCTGGAT
  361 CTCCCTGGAG GTGATGGTCG AGCGCTTGTT GTAATGCGCC AGGCGGGAAG CCTCACCTGC
  421 GATGCGCTCG AAAATGTCGT TCACAAACGA ATTCATGATG CCCATGGCCT TGGACGAAAT
  481 GCCGGTGTCA GGGTGGACCT GCTTCAGAAC CTTGTACACA TAGATGGAAT AGCTCTCCTT
  541 GCGGCTGCGC TTGCGCTTCT TGCCGCCTTT CTTCTGCGCC TTAGTCACCG CCTTCTTGGA
  601 GCCCTTTTTC GGGGCGGGAG CAGACTTCGC TGGCTCTGGC ATTGGGCCAG GATTCTCCTC
  661 GACGTCACCG CATGTTAGAA GACTTCCTCT GCCCTCGCTA GACAGAGAAA TGAAGTCCAG
  721 GGCTTGGAGG AGGGAGAGGG AAAGCANCNN NAGCAGCATN NNNNATGTGT TCTGAACCAG
  781 CAGGCTTATG CCGCCATTCT CGAGTTGNNA GCTAGACGAT CCGCCACCGC CAGAGCCACC
  841 TCCGCCTGAA CCGCCTCCAC CGCNAGACGA TCCGCCACNG CCAGANCCAC CTCCGCCTGN
  901 ACCGNCTNCN CCGCTAGACG ATCCGCCNNC GCNNGANCCA NCTCCGCNTG ANCCGCCNNC
  961 NCCGCTAGCC TTCTGGNNCA GCTCCNNNTN CNGGNNNNGA NCTNCNNTNN NANNTGGGCG
1021 TNNNNCTNNT NNNNGGNTNG NAGCTNNNTT TNCNAANCNN NNNNNNNN
//
Primer: HIV-B_R (Pr0673)
Region: HIV-1 psi, 5'LTR
Sequence file: RDB15871_A8Cdf.seq check
>D05101A3_A8Cd_2_HIV-B_R_D01_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN ANNTTTTGGN GNACTCNCNN NGTCGCCGCC CCTCGCCTCT
   61 TGCCGTGCGC GCTTCAGCAA GCCGAGTCCT GCGTCGAGAG AGCTCCTCTG GTTTCCCTTT
  121 CGCTTTCAAG TCCCTGTTCG GGCGCCACTG CTAGAGATTT TCCACACTGA CTAAAAGGGT
  181 CTGAGGGATC TCTAGTTACC AGAGTCACAC AACAGACGGG CACACACTAC TTGAAGCACT
  241 CAAGGCAAGC TTTATTGAGG CTTAAGCAGT GGGTTCCCTA GTTAGCCAGA GAGCTCCCAG
  301 GCTCAGATCT GGTCTAACCA GAGAGACCCA GTACAGGCAA AACGCGCTGC TTATATAGAC
  361 CTCCCACCGT ACACGCCTAC CGCCCATTTG CGTCAATGGG GCGGAGTTGT TACGACATTT
  421 TGGAAAGTCC CGTTGATTTT GGTGCCAAAA CAAACTCCCA TTGACGTCAA TGGGGTGGAG
  481 ACTTGGAAAT CCCCGTGAGT CAAACCGCTA TCCACGCCCA TTGATGTACT GCCAAAACCG
  541 CATCACCATG GTAATAGCGA TGACTAATAC GTAGATGTAC TGCCAAGTAG GAAAGTCCCA
  601 TAAGGTCATG TACTGGGCAT AATGCCAGGC GGGCCATTTA CCGTCATTGA CGTCAATAGG
  661 GGGCGTACTT GGCATATGAT ACACTTGATG TACTGCCAAG TGGGCAGTTT ACCGNNNNNC
  721 TCCACCCATT GACGTCAATG GAAAGTCCCT ATTGGCGTTA CTATGNGAAC ATACGTCATT
  781 ATTGACGTCN ATGGGCGGGG GTCGTTGGGC GGNCAGCCAG GNGGGNCATT TANCGTNNGN
  841 NATGTANCGC GNAACTNCNT ATATGGGCNN TGAACTNANG ACCCCGTAAT TGANTACTNN
901 NNNNNNNTAN NCNNNNNNNN NN
//
Primer: BGH_rev2 (Pr0606)
Region: 3'LTR
Sequence file: RDB15871_A8Cdg.seq check
>D05101A3_2_A8Cd_BGH_rev2_E02_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGNGGNNGG GCNNNCNACA GNTGGCTGGC AANTAGAAGG
   61 TACAGTCGAG GCNNNNNNNN NNNNNNNNAC GGGCCCTGCT AGAGATTTTC CACANNNNNN
  121 NNAAGGGTCT GAGGGATCTC TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT
  181 GAAGCACTCA AGGCAAGCTT TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA
  241 GCTCCCAGGC TCAGATCTGG TCTAACCAGA GAGACCCAGT ACAGTCCGGA TGCAGCTCTC
  301 GGGCCATGTG ATGAAATGCT AGGCGGCTGT CAAACCTCCA CTCTAATACT TCTCTCTCCG
  361 GGTCATCCAT CCCATGCAGG CTCACAGGGT GTAACAAGCG GGTGTTCTCT CCTTCATTGG
  421 CTTCTTCTAC CTTCTCTTGC TCAACTGGTA CTAGCTTGTA GCACCATCCA AAGGTCAGTG
  481 GATATCTGAT CCCTGGCCCT GGTGTGTAGT TCTGCCAATC AGGGAAGTAG CCTTGTGTGT
  541 GGTAGATCCA CAGATCAAGG ATATCTTGTC TTCGTTGGGA GTGAATTAGC CCTTCCAGTC
  601 CCCCCTTTTC TTTTAAAAAG TGGCTAAGAT CTACAGCTGC CTTGTAAGTC ATTGGTCTTA
  661 AAGGTACCTG AGGTGTGACT GGAAAACCCA CCTCCTCCTC CTCTTGTGCT TCTAGCCAGG
  721 CACAATCAGC ATTGGTAGCT GCTGTATTGC TACTTGTGAT TGCTCCATGT TTTTCTANGT
  781 CTCGACGGTN TCGATGCNGG GNNGCGGNCC AAAGGGANAT NCGANTCGTC TGANGGCNAA
  841 NGCGAANANN CNNAANANGN CGCANANNCN NNNNCNNNCC GCGGGAANGN ANGNNCNNNN
901 NNNTGANGGN CNNANNGNNN NNNNNNN
//
Primer: SV40pro_F (Pr0110)
Region: EM7 pro, BleoR, SV40 pA
Sequence file: RDB15871_A8Cdh.seq check
>D05101A3_2_A8Cd_SV40pro_F_F02_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNANNGN GGNNGCTTTT TTGGAGGCCT AGGCTTTTGC
   61 NAAAAGCTCC CGGGAGCTTG NATATCCATT TTCGGATCTG ATCAGCACGT GTTGACAATT
  121 AATCATCGGC ATAGTATATC GGCATAGNAT NNNACGACAA GGTGAGGAAC TAAACCATGG
  181 CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA CCGCGCGCGA CGTCGCCGGA GCGGTCGAGT
  241 TCTGGACCGA CCGGCTCGGG TTCTCCCGGG ACTTCGTGGA GGACGACTTC GCCGGTGTGG
  301 TCCGGGACGA CGTGACCCTG TTCATCAGCG CGGTCCAGGA CCAGGTGGTG CCGGACAACA
  361 CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG ACGAGCTGTA CGCCGAGTGG TCGGAGGTCG
  421 TGTCCACGAA CTTCCGGGAC GCCTCCGGGC CGGCCATGAC CGAGATCGGC GAGCAGCCGT
  481 GGGGGCGGGA GTTCGCCCTG CGCGACCCGG CCGGCAACTG CGTGCACTTC GTGGCCGAGG
  541 AGCAGGACTG ACACGTGCTA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG
  601 GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC
  661 TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA
  721 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCNAGT TGTGGTTTGT
  781 CCAAACTCAT CNATGTATCT TATCATGTCT GTATACNGTC NACCTCTANC TAGAGCTNGG
  841 CGTAATCATG GNCATAGCTG TTNNCNGNGT GAAATTGTTA TCCGCTCACA ANNNCNCNCA
  901 ACATACGANN NNGNAAGNNT AAAGTGTAAA GNCNNNNGNN NNCNNNANNG ANTGNNNNNN
961 NNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kinoshita, N., Genetically Encoded Fluorescent Indicator GRAPHIC Delineates Intercellular Connections. iScience 15: 28-38 (2019). PMID 31026667. [link to RRC of NBRP]