RDB15871 - pCSII-CMV-MCS_B (BFP type) n-GRAPHIC T2A H2B-mCherry

Sequence (full)	RDB15871zzt01.seq compiled by us
Sequence (partial)	RDB15871pka01.seq provided by the depositor

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Kinoshita, N. et al., iScience 15: 28-38 (2019)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. Additional terms and conditions: The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
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Remarks	Remember that you will be working with samples containing infectious virus. This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Kinoshita, N. et al., iScience 15: 28-38 (2019)) を引用し、謝辞の表明を必要とする。本件リソースの使用は学術機関での学術研究に限る。付加的提供条件：レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15871	pCSII-CMV-MCS_B (BFP type) n-GRAPHIC T2A H2B-mCherry	DNA solution

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check

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Primer: YFP_R (Pr0127) Region: insert 5', CMV pro_2, CMV en_2 Sequence file: RDB15871_A8Cda.seq
>D05101A3_A8Cd_2_YFP_R_A01_01_ABI24.ab1 1 NNNNNNNNNN NNNNNNNCCN NGNNNGCNNN NTTGTGGCCG TTTACGTCGC CGTCCNGCTC 61 GACCAGGATG GGCACCACCC CGGTGAACAG CTCCTCGCCC TTGCTCACTC TAGCCTGCAG 121 CGTGGTGTTA TCCTTGGCAA CCACGGACAC TGCCAAGACC AGCACAGCGA CAGTTGGCAG 181 CATCTCTACC ATGGTGGCTC TAGCGGATCT GACGGTTCAC TAAACCAGCT CTGCTTATAT 241 AGACCTCCCA CCGTACACGC CTACCGCCCA TTTGCGTCAA TGGGGCGGAG TTGTTACGAC 301 ATTTTGGAAA GTCCCGTTGA TTTTGGTGCC AAAACAAACT CCCATTGACG TCAATGGGGT 361 GGAGACTTGG AAATCCCCGT GAGTCAAACC GCTATCCACG CCCATTGATG TACTGCCAAA 421 ACCGCATCAC CATGGTAATA GCGATGACTA ATACGTAGAT GTACTGCCAA GTAGGAAAGT 481 CCCATAAGGT CATGTACTGG GCATAATGCC AGGCGGGCCA TTTACCGTCA TTGACGTCAA 541 TAGGGGGCGT ACTTGGCATA TGATACACTT GATGTACTGC CAAGTGGGCA GTTTACCGTA 601 AATACTCCAC CCATTGACGT CAATGGAAAG TCCCTATTGG CGTTACTATG GGAACATACG 661 TCATTATTGA CGTCAATGGG CGGGGGTCGT TGGGCGGTCA GCCAGGCGGG CCATTTACCG 721 TAAGTTATGT AACGCGGANC TCCATATATG GGCTATGAAC TAATGNNCCC NTAATTGATT 781 ACTATTAATC GAATTAATTC NNGTGAATTT CTGCTGTCCC TGTAATAAAC CNNAAAATTT 841 TGAATTTTTG TAATTTGTTT TTGTAANTCT TTANTTTGTA TGTCNGTTGC TANTATGTCN 901 ACNANTCTTT CCCCNGCNCT NNACCCCCNN NNNNCCCNCN NTTTNNTTNA AAANTNGNNG 961 NNNNNNNNNN //
Primer: PRE_rev (Pr0250) Region: PRE, insert 3' Sequence file: RDB15871_A8Cdb.seq
>D05101A3_2_A8Cd_PRE_rev_D02_11_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNCNNN ATAGTTAAGA ATACCAGTCA ATCTTTCNCA 61 AATTTTGTAA TCCAGAGGTT GNTTATCGAT AAGCTTGATC CCTCGATGTT AACTCTAGAT 121 TACTTGTACA GCTCGTCCAT GCCGCCGGTG GAGTGGCGGC CCTCGGCGCG TTCGTACTGT 181 TCCACGATGG TGTAGTCCTC GTTGTGGGAG GTGATGTCCA ACTTGATGTT GACGTTGTAG 241 GCGCCGGGCA GCTGCACGGG CTTCTTGGCC TTGTAGGTGG TCTTGACCTC AGCGTCGTAG 301 TGGCCGCCGT CCTTCAGCTT CAGCCTCTGC TTGATCTCGC CCTTCAGGGC GCCGTCCTCG 361 GGGTACATCC GCTCGGAGGA GGCCTCCCAG CCCATGGTCT TCTTCTGCAT TACGGGGCCG 421 TCGGAGGGGA AGTTGGTGCC GCGCAGCTTC ACCTTGTAGA TGAACTCGCC GTCCTGCAGG 481 GAGGAGTCCT GGGTCACGGT CACCACGCCG CCGTCCTCGA AGTTCATCAC GCGCTCCCAC 541 TTGAAGCCCT CGGGGAAGGA CAGCTTCAAG TAGTCGGGGA TGTCGGCGGG GTGCTTCACG 601 TAGGCCTTGG AGCCGTACAT GAACTGAGGG GACAGGATGT CCCAGGCGAA NGGCAGGGGG 661 CCACCCTTGG TCACCTTCAG CTTGGCGGTC TGGGTGCCCT CGTAGGGGCG GCCCTCGCCC 721 TCGCCCTCGA TCTCGAACTC GTGGCCGTTC ACGGANCCCT CCATGTGCAC CTTGAAGCGC 781 ATGAACTCCT TGATGATGGC CATGTTATCC TCCTCGCCNN TGCTCACCAT GGNGNCTGNN 841 TCCTTANCGC TGGTGTACTT GGNGATNGNC TTANNANCNN CGGANACGGN GNGCTNNNNN 901 NNNTCNNNNN GNANCAGCNN GNNNNCNNNN NNNNTGGNTN NNCNNNNNNN NNN //
Primer: CSII-CMV5' (Pr0021) Region: insert mid Sequence file: RDB15871_A8Cdc.seq
>D05101A3_A8Cd_2_CSII-CMV5'_F01_16_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNTGTCNNNN NNCNNNGNCT 61 TNNNNNNNGN NCGTGGTTGC CNNNGNTAAC ACCACGCTGC AGGCTAGAGT GAGCAAGGGC 121 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC 181 CACAAGTTCA GCGTGCGCGG CGAGGGCGAG GGCGATGCCA CCAACGGCAA GCTGACCCTG 241 AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG 301 AGCCACGGCG TGCAGTGCTT CGCCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC 361 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTCCTTCAA GGACGACGGC 421 ACCTACAAGA CCCGCGCCGT TGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG 481 CTGAAGGGCG TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGNNNNNN 541 TTCAACAGCC ACNNCATCTA TATCACGGCC GTCAAGCAGG CTAGAGGTGG AGGCGGTTCA 601 GGCGGAGGTG GCTCTGGCGG TGGCGGATCG GCTAGCGCCA CCATGGCCCA GCTCGAAAAG 661 GANCTGCAAG CCCTGGNAGA ANGAGAANGC CCAGCTCGAA TGGGAGCTCC ANGCCCTGGA 721 GAANGAGCTG GNCCNNAANG CTAGCGGTGG AGGCNNNTCA GGCGGANGTG GCTCTGGCGG 781 NNGCGGATCG TCTANCGGNG GANGCGNNTC ANGCGGAGGT GGNTCTGGCG GTGGCGNNTC 841 NNCTNNCGGT GGANGNNGNN TNCAGGCGGA NGTGGCTCTG GNGGNNGNNG GATCGTCTAG 901 CTACCNAANT CGAGAATGGC NGCNTANNCC NTGCTGGNTT CNNAANNNNT NCNNGNNNNG 961 CNNNCNNGCT GCTGNNNTTT CCNNNNNCCC NNNNNNNCNA NGCCCNTNNA CNTNNATTTN 1021 NNNNNNNCNN NNNNNNNGGN NNNNANNNNN NNNNN //
Primer: VN_F1 (Pr0992) Region: insert mid Sequence file: RDB15871_A8Cdd.seq
>D05101A3_A8Cd_2_VN_F1_A01_01_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NTCTATATNN CGGCCGNCNN NNANNGCTAG 61 AGGTGGAGGC GGTTCAGGCG GAGGTGGCTC TGGCGGTGGC GGATCGGCTA GCGCCACCAT 121 GGCCCAGCTC GAAAAGGAGC TGCAAGCCCT GGAGAAGGAG AACGCCCAGC TCGAATGGGA 181 GCTCCAGGCC CTGGAGAAGG AGCTGGCCCA GAAGGCTAGC GGTGGAGGCG GTTCAGGCGG 241 AGGTGGCTCT GGCGGTGGCG GATCGTCTAG CGGTGGAGGC GGTTCAGGCG GAGGTGGCTC 301 TGGCGGTGGC GGATCGTCTA GCGGTGGAGG CGGTTCAGGC GGAGGTGGCT CTGGCGGTGG 361 CGGATCGTCT AGCTACCAAC TCGAGAATGG CGGCATAAGC CTGCTGGTTC AGAACACATC 421 CTGGATGCTG CTGCTGCTGC TTTCCCTCTC CCTCCTCCAA GCCCTGGACT TCATTTCTCT 481 GTCTAGCGAG GGCAGAGGAA GTCTTCTAAC ATGCGGTGAC GTCGAGGAGA ATCCTGGCCC 541 AATGCCAGAG CCAGCGAAGT CTGCTCCCGC CCCGAAAAAG GGCTCCAAGA AGGCGGTGAC 601 TAAGGCGCAN AAGAAAGGCG GCAAGAAGCG CAAGCGCAGC CGCAAGGAGA GCTATTCCAT 661 CTATGTGTAC AAGGTTCTGA AGCAGGTCCA CCCTGACACC GGCATTTCGT CCAAGGCCAT 721 GGGCATCATG AATTCGTTTG TGAACGACAT TTTCGAGCGC ATCGCAGGTG AGGCTTCCCG 781 CCTGGCGCAT TACANCAAGC GCTCGACCAT CACCTCNAGG GAGATCCAGA NNGNCGTGCG 841 CCTGCTGCTG NCNGGGGAGT TGGNCNAGCA CGCCGTGTCC GANGGTACTA AGGCCATCNN 901 NNAGTACACC AGCGCNNANG ANCCNGCCAC CNTNNTGANN ANGGGCGNNG ANGANAACAT 961 GGNCNTCATN NANNNANTTC NTGCGCTTCN NNGNGNNCAT GNNNGGCTNC NNNNNNNGGC 1021 CNCNANTNCN AGATNNAGGN NNANNNNNAN GNNNNNNCNT NNNNNNGNNN NCNNNNNNNN 1081 NN //
Primer: mRFP-M_R (Pr0674) Region: insert mid Sequence file: RDB15871_A8Cde.seq
>D05101A3_A8Cd_2_mRFP-M_R_B01_02_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GGNNCCGNNN NNGAACTGAG GGGANAGGNN 61 GTCCCAGGCG AAGGGCAGGG GGCCACCCTT GGTCACCTTC AGCTTGGCGG TCTGGGTGCC 121 CTCGTAGGGG CGGCCCTCGC CCTCGCCCTC GATCTCGAAC TCGTGGCCGT TCACGGAGCC 181 CTCCATGTGC ACCTTGAAGC GCATGAACTC CTTGATGATG GCCATGTTAT CCTCCTCGCC 241 CTTGCTCACC ATGGTGGCTG GATCCTTAGC GCTGGTGTAC TTGGTGATGG CCTTAGTACC 301 CTCGGACACG GCGTGCTTGG CCAACTCCCC AGGCAGCAGC AGGCGCACGG CCGTCTGGAT 361 CTCCCTGGAG GTGATGGTCG AGCGCTTGTT GTAATGCGCC AGGCGGGAAG CCTCACCTGC 421 GATGCGCTCG AAAATGTCGT TCACAAACGA ATTCATGATG CCCATGGCCT TGGACGAAAT 481 GCCGGTGTCA GGGTGGACCT GCTTCAGAAC CTTGTACACA TAGATGGAAT AGCTCTCCTT 541 GCGGCTGCGC TTGCGCTTCT TGCCGCCTTT CTTCTGCGCC TTAGTCACCG CCTTCTTGGA 601 GCCCTTTTTC GGGGCGGGAG CAGACTTCGC TGGCTCTGGC ATTGGGCCAG GATTCTCCTC 661 GACGTCACCG CATGTTAGAA GACTTCCTCT GCCCTCGCTA GACAGAGAAA TGAAGTCCAG 721 GGCTTGGAGG AGGGAGAGGG AAAGCANCNN NAGCAGCATN NNNNATGTGT TCTGAACCAG 781 CAGGCTTATG CCGCCATTCT CGAGTTGNNA GCTAGACGAT CCGCCACCGC CAGAGCCACC 841 TCCGCCTGAA CCGCCTCCAC CGCNAGACGA TCCGCCACNG CCAGANCCAC CTCCGCCTGN 901 ACCGNCTNCN CCGCTAGACG ATCCGCCNNC GCNNGANCCA NCTCCGCNTG ANCCGCCNNC 961 NCCGCTAGCC TTCTGGNNCA GCTCCNNNTN CNGGNNNNGA NCTNCNNTNN NANNTGGGCG 1021 TNNNNCTNNT NNNNGGNTNG NAGCTNNNTT TNCNAANCNN NNNNNNNN //
Primer: HIV-B_R (Pr0673) Region: HIV-1 psi, 5'LTR Sequence file: RDB15871_A8Cdf.seq
>D05101A3_A8Cd_2_HIV-B_R_D01_10_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN ANNTTTTGGN GNACTCNCNN NGTCGCCGCC CCTCGCCTCT 61 TGCCGTGCGC GCTTCAGCAA GCCGAGTCCT GCGTCGAGAG AGCTCCTCTG GTTTCCCTTT 121 CGCTTTCAAG TCCCTGTTCG GGCGCCACTG CTAGAGATTT TCCACACTGA CTAAAAGGGT 181 CTGAGGGATC TCTAGTTACC AGAGTCACAC AACAGACGGG CACACACTAC TTGAAGCACT 241 CAAGGCAAGC TTTATTGAGG CTTAAGCAGT GGGTTCCCTA GTTAGCCAGA GAGCTCCCAG 301 GCTCAGATCT GGTCTAACCA GAGAGACCCA GTACAGGCAA AACGCGCTGC TTATATAGAC 361 CTCCCACCGT ACACGCCTAC CGCCCATTTG CGTCAATGGG GCGGAGTTGT TACGACATTT 421 TGGAAAGTCC CGTTGATTTT GGTGCCAAAA CAAACTCCCA TTGACGTCAA TGGGGTGGAG 481 ACTTGGAAAT CCCCGTGAGT CAAACCGCTA TCCACGCCCA TTGATGTACT GCCAAAACCG 541 CATCACCATG GTAATAGCGA TGACTAATAC GTAGATGTAC TGCCAAGTAG GAAAGTCCCA 601 TAAGGTCATG TACTGGGCAT AATGCCAGGC GGGCCATTTA CCGTCATTGA CGTCAATAGG 661 GGGCGTACTT GGCATATGAT ACACTTGATG TACTGCCAAG TGGGCAGTTT ACCGNNNNNC 721 TCCACCCATT GACGTCAATG GAAAGTCCCT ATTGGCGTTA CTATGNGAAC ATACGTCATT 781 ATTGACGTCN ATGGGCGGGG GTCGTTGGGC GGNCAGCCAG GNGGGNCATT TANCGTNNGN 841 NATGTANCGC GNAACTNCNT ATATGGGCNN TGAACTNANG ACCCCGTAAT TGANTACTNN 901 NNNNNNNTAN NCNNNNNNNN NN //
Primer: BGH_rev2 (Pr0606) Region: 3'LTR Sequence file: RDB15871_A8Cdg.seq
>D05101A3_2_A8Cd_BGH_rev2_E02_14_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNGNGGNNGG GCNNNCNACA GNTGGCTGGC AANTAGAAGG 61 TACAGTCGAG GCNNNNNNNN NNNNNNNNAC GGGCCCTGCT AGAGATTTTC CACANNNNNN 121 NNAAGGGTCT GAGGGATCTC TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT 181 GAAGCACTCA AGGCAAGCTT TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA 241 GCTCCCAGGC TCAGATCTGG TCTAACCAGA GAGACCCAGT ACAGTCCGGA TGCAGCTCTC 301 GGGCCATGTG ATGAAATGCT AGGCGGCTGT CAAACCTCCA CTCTAATACT TCTCTCTCCG 361 GGTCATCCAT CCCATGCAGG CTCACAGGGT GTAACAAGCG GGTGTTCTCT CCTTCATTGG 421 CTTCTTCTAC CTTCTCTTGC TCAACTGGTA CTAGCTTGTA GCACCATCCA AAGGTCAGTG 481 GATATCTGAT CCCTGGCCCT GGTGTGTAGT TCTGCCAATC AGGGAAGTAG CCTTGTGTGT 541 GGTAGATCCA CAGATCAAGG ATATCTTGTC TTCGTTGGGA GTGAATTAGC CCTTCCAGTC 601 CCCCCTTTTC TTTTAAAAAG TGGCTAAGAT CTACAGCTGC CTTGTAAGTC ATTGGTCTTA 661 AAGGTACCTG AGGTGTGACT GGAAAACCCA CCTCCTCCTC CTCTTGTGCT TCTAGCCAGG 721 CACAATCAGC ATTGGTAGCT GCTGTATTGC TACTTGTGAT TGCTCCATGT TTTTCTANGT 781 CTCGACGGTN TCGATGCNGG GNNGCGGNCC AAAGGGANAT NCGANTCGTC TGANGGCNAA 841 NGCGAANANN CNNAANANGN CGCANANNCN NNNNCNNNCC GCGGGAANGN ANGNNCNNNN 901 NNNTGANGGN CNNANNGNNN NNNNNNN //
Primer: SV40pro_F (Pr0110) Region: EM7 pro, BleoR, SV40 pA Sequence file: RDB15871_A8Cdh.seq
>D05101A3_2_A8Cd_SV40pro_F_F02_17_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNANNGN GGNNGCTTTT TTGGAGGCCT AGGCTTTTGC 61 NAAAAGCTCC CGGGAGCTTG NATATCCATT TTCGGATCTG ATCAGCACGT GTTGACAATT 121 AATCATCGGC ATAGTATATC GGCATAGNAT NNNACGACAA GGTGAGGAAC TAAACCATGG 181 CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA CCGCGCGCGA CGTCGCCGGA GCGGTCGAGT 241 TCTGGACCGA CCGGCTCGGG TTCTCCCGGG ACTTCGTGGA GGACGACTTC GCCGGTGTGG 301 TCCGGGACGA CGTGACCCTG TTCATCAGCG CGGTCCAGGA CCAGGTGGTG CCGGACAACA 361 CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG ACGAGCTGTA CGCCGAGTGG TCGGAGGTCG 421 TGTCCACGAA CTTCCGGGAC GCCTCCGGGC CGGCCATGAC CGAGATCGGC GAGCAGCCGT 481 GGGGGCGGGA GTTCGCCCTG CGCGACCCGG CCGGCAACTG CGTGCACTTC GTGGCCGAGG 541 AGCAGGACTG ACACGTGCTA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG 601 GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC 661 TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA 721 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCNAGT TGTGGTTTGT 781 CCAAACTCAT CNATGTATCT TATCATGTCT GTATACNGTC NACCTCTANC TAGAGCTNGG 841 CGTAATCATG GNCATAGCTG TTNNCNGNGT GAAATTGTTA TCCGCTCACA ANNNCNCNCA 901 ACATACGANN NNGNAAGNNT AAAGTGTAAA GNCNNNNGNN NNCNNNANNG ANTGNNNNNN 961 NNNN //

pCSII-CMV-MCS_B (BFP type) n-GRAPHIC T2A H2B-mCherry

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QC test results

References