Lentiviral expression vector of GRAPHIC probe (LZA).
Clone info. | Lentiviral expression vector of GRAPHIC probe (GPI anchored Reconstitution-Activated Proteins Highlight Intercellular Connections), fluorescent indicator for intercellular contacts with optimized intercellular GFP reconstitution. This probe contains an mouse preproacrosin signal peptide (SP), superfolder GFP-NT, acidic leucine zipper domain (LZA), mouse Thy-1 GPI-anchored domain, 2A peptide, human histone 2B (H2B), mCherry, see Fig. 1 of Kinoshita, N. et al., iScience 15: 28-38, 2019. |
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Comment | Expression was confirmed by the depositor with detection of reconstituted GFP fluorescence and nucleic mCherry fluorecence. |
Vector backbone | CSII-CMV-MCS (RDB04377) (plasmid) |
Size of vector backbone | 8.6 kb |
Selectable markers | Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. |
Gene/insert name | mouse Acr cDNA mouse Thy1 cDNA human histon H2BJ cDNA Aequorea victoria GFP cDNA Discosoma sp. RFP cDNA |
Depositor|Developer | Shimogori, Tomomi | Kinoshita, Nagatoki | |
Sequence (full) | RDB15870hts01.seq ![]() |
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Publication | Kinoshita, N., iScience 15: 28-38 (2019). [link to RRC of NBRP] |
Test sheet | RDB15870_B3B3p1-2.pdf ![]() |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose)[Word ![]() ![]() Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Kinoshita, N. et al., iScience 15: 28-38 (2019)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. Additional terms and conditions: The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector. |
Remarks | Remember that you will be working with samples containing infectious virus. This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利機関による非営利学術研究用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Kinoshita, N. et al., iScience 15: 28-38 (2019)) を引用し、謝辞の表明を必要とする。本件リソースの使用は学術機関での学術研究に限る。 MTAに書く付加的使用条件: レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。 |
備考 | このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB15870 | pCSII-CMV-MCS_n-GRAPHIC T2A H2B-mCherry | DNA solution |
Materials & Methods section:
The pCSII-CMV-MCS_n-GRAPHIC T2A H2B-mCherry was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15870). |
Reference section:
Kinoshita, N., Huang, A.J.Y., McHugh, T.J., Suzuki, S.C., Masai, I., Kim, I.H., Soderling, S.H., Miyawaki, A., Shimogori, T., Genetically Encoded Fluorescent Indicator GRAPHIC Delineates Intercellular Connections. iScience 15: 28-38 (2019). PMID 31026667. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Original, user report and related articles
original | Kinoshita, N., Genetically Encoded Fluorescent Indicator GRAPHIC Delineates Intercellular Connections. iScience 15: 28-38 (2019). PMID 31026667. [link to RRC of NBRP] |
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