RDB15870 - pCSII-CMV-MCS_n-GRAPHIC T2A H2B-mCherry

Sequence (full)	RDB15870zzt02.seq compiled by us
Sequence (partial)	RDB15870pka01.seq provided by the depositor

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Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Kinoshita, N. et al., iScience 15: 28-38 (2019)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. Additional terms and conditions: The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
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Remarks	Remember that you will be working with samples containing infectious virus. This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Kinoshita, N. et al., iScience 15: 28-38 (2019)) を引用し、謝辞の表明を必要とする。本件リソースの使用は学術機関での学術研究に限る。付加的提供条件：レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15870	pCSII-CMV-MCS_n-GRAPHIC T2A H2B-mCherry	DNA solution

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check

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Primer: EGFP-N_R2 (Pr0905) Region: insert 5',CMV pro.2,CMV en.2 Sequence file: RDB15870_A8Cdh.seq
>D05101A2_A8Cd_2_EGFP-N_R2_E01_13_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNCN NNNNNNNNNC NCNCCNTNNG TCANGGNGGT 61 CACGANGGTG GGCCAGGGCA CGGGCAGCTT GCCGGTGGTG CAGATGAACT TCAGGGTCAG 121 CTTGCCGATG GTGGCATCGC CCTCGCCCTC GCCGCGCACG CTGAACTTGT GGCCGTTTAC 181 GTCGCCGTCC AGCTCGACCA GGATGGGCAC CACCCCGGTG AACAGCTCCT CGCCCTTGCT 241 CACTCTAGCC TGCAGCGTGG TGTTATCCTT GGCAACCACG GACACTGCCA AGACCAGCAC 301 AGCGACAGTT GGCAGCATCT CTACCATGGT GGCTCTAGCG GATCTGACGG TTCACTAAAC 361 CAGCTCTGCT TATATAGACC TCCCACCGTA CACGCCTACC GCCCATTTGC GTCAATGGGG 421 CGGAGTTGTT ACGACATTTT GGAAAGTCCC GTTGATTTTG GTGCCAAAAC AAACTCCCAT 481 TGACGTCAAT GGGGTGGAGA CTTGGAAATC CCCGTGAGTC AAACCGCTAT CCACGCCCAT 541 TGATGTACTG CCAAAACCGC ATCACCATGG TAATAGCGAT GACTAATACG TAGATGTACT 601 GCCAAGTAGG AAAGTCCCAT AAGGTCATGT ACTGGGCATA ATGCCAGGCG GGCCATTTAC 661 CGTCATTGAC GTCAATAGGG GGCGTACTTG GCATATGATA CACTTGATGT ACTGNCAAGT 721 GGGCAGTTTA CCGTAAATAC TCCACCCATT GACGTCAATG GAAAGTCCCT ATTGGCGTTA 781 CTATGGGAAC ATACGTCNTT ATTGACGTCA ATGNGCGGGG GTCGTNNGGC GGNNCAGCCA 841 GGCGGGNNAT TTANCGTAAG TTATGTAACG CGGAACTNCN TATATGGGCT ATGANCTNAT 901 GACCCCGNNA TTGATTACTA TTAATCNNAN TNNNTNCNAA GNGNATTTCT GCTGNNCCNN 961 NNNNNAANCN NAAAAANTTT NNANTTTTTG NNNNTNGNTT TNNNNNNNNN TNNNNNNNNN 1021 N //
Primer: PRE_rev (Pr0250) Region: insert 3' Sequence file: RDB15870_A8Cdi.seq
>D05101A2_2_A8Cd_PRE_rev_G01_19_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNGNNCANC ATAGTTAAGA ATACCAGTCA ATCTTTCNCA 61 AATTTTGTAA TCCAGAGGTT GNTTATCGAT AAGCTTGATC CCTCGATGTT AACTCTAGAT 121 TACTTGTACA GCTCGTCCAT GCCGCCGGTG GAGTGGCGGC CCTCGGCGCG TTCGTACTGT 181 TCCACGATGG TGTAGTCCTC GTTGTGGGAG GTGATGTCCA ACTTGATGTT GACGTTGTAG 241 GCGCCGGGCA GCTGCACGGG CTTCTTGGCC TTGTAGGTGG TCTTGACCTC AGCGTCGTAG 301 TGGCCGCCGT CCTTCAGCTT CAGCCTCTGC TTGATCTCGC CCTTCAGGGC GCCGTCCTCG 361 GGGTACATCC GCTCGGAGGA GGCCTCCCAG CCCATGGTCT TCTTCTGCAT TACGGGGCCG 421 TCGGAGGGGA AGTTGGTGCC GCGCAGCTTC ACCTTGTAGA TGAACTCGCC GTCCTGCAGG 481 GAGGAGTCCT GGGTCACGGT CACCACGCCG CCGTCCTCGA AGTTCATCAC GCGCTCCCAC 541 TTGAAGCCCT CGGGGAAGGA CAGCTTCAAG TAGTCGGGGA TGTCGGCGGG GTGCTTCACG 601 TAGGCCTTGG AGCCGTACAT GAACTGAGGG GACAGGATGT CCCAGGCGAA GGGCAGGGGG 661 CCACCCTTGG TCACCTTCAG CTTGGCGGTC TGGGTGCCCT CGTAGGGGCG GCCCTCGCCC 721 TCGCCCTCGA TCTCGAACTC GTGGCCGTTC ACGGAGCCCT CCATGTGCAC CTTGAAGCGC 781 ATGANCTCCT TGATGATGGC CATGTTATCC TCCTCGCCCT TGCTCACCAT GGTGGCTGGA 841 TCCTTAGCGC TNNTGTACTT GGNGATGGNC TTANNANCCT CNGANACGGN GNNCTNGGNC 901 NANTNCNNNN GNANCANCNN NNNNNNNGNN NNNNTGGNNN NNCCNNGNNN NNNNNNGNNN 961 NNNNNNN //
Primer: HIV-B_R (Pr0673) Region: HIV-1 psi,5' LTR,CMV pro.1,CMV en.1 Sequence file: RDB15870_A8Cdj.seq
>15870_15870_B3D4F_HIV-B_R_G01_07_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNANT TTTGNNNNNN NCNCCAGTCG CCGCCCCTCG 61 CCTCTTGCCG TGCGCGCTTC ANCAAGCCGA GTCCTGCGTC GAGAGAGCTC CTCTGGTTTC 121 CCTTTCGCTT TCAAGTCCCT GTTCGGGCGC CACTGCTAGA GATTTTCCAC ACTGACTAAA 181 AGGGTCTGAG GGATCTCTAG TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA 241 GCACTCAAGG CAAGCTTTAT TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT 301 CCCAGGCTCA GATCTGGTCT AACCAGAGAG ACCCAGTACA GGCAAAACGC GCTGCTTATA 361 TAGACCTCCC ACCGTACACG CCTACCGCCC ATTTGCGTCA ATGGGGCGGA GTTGTTACGA 421 CATTTTGGAA AGTCCCGTTG ATTTTGGTGC CAAAACAAAC TCCCATTGAC GTCAATGGGG 481 TGGAGACTTG GAAATCCCCG TGAGTCAAAC CGCTATCCAC GCCCATTGAT GTACTGCCAA 541 AACCGCATCA CCATGGTAAT AGCGATGACT AATACGTAGA TGTACTGCCA AGTAGGAAAG 601 TCCCATAAGG TCATGTACTG GGCATAATGC CAGGCGGGCC ATTTACCGTC ATTGACGTCA 661 ATAGGGGGCG TACTTGGCAT ATGATACACT TGATGTACTG CCAAGTGGGC AGTTTACCGT 721 AAATACTCCA CCCATTGACG TCAATGGAAA GTCCCTATTG GCGTTACTAT GGGAANNNTA 781 CGTCATTATT GACGTCNATG GGCGGGGGTC GTTGGGCGGT CAGCCAGGCG GGCCATTTAC 841 CGNAAGTTAT GTNACGCGNA NNTCCNTATA TGGGCTATGA ACNAATGANC CCGNAATNGA 901 NNNNCTATTA ATANNTNNTC AATANNCANT GNCNACGCNN ATATCTGGNC CNGTACNTCG 961 CGAANNANCN CAAAACGCCT AANCCTAANN ANANTCTTCN TGNANTNGNC NGNCANNNNN 1021 NGNNTNNNNN ANCTNAAATT TNGNTCNCGC NNNANNCNNN NNNCNNNNNN NNNN //
Primer: HIVgp_R1 (Pr0132) Region: HIV-1 psi,5' LTR Sequence file: RDB15870_A8Cdk.seq
>D05101A2_2_A8Cd_HIVgp-R1_B02_05_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNC NCTTTTTTCT CTCTGCACCN CTCTTCTCTT TGCCTTGGTG 61 GGTGCTACTC CTAATGGTTC NATTTTTACT ACTTTATATT TATATAATTC ACTTCTCCAA 121 TTGTCCCTCA TATCTCCTCC TCCAGGTCTG AAGATCAGCG GCCGGCCGCT TGCTGTGCGG 181 TGGTCTTACT TTTGTTTTGC TCTTCCTCTA TCTTGTCTAA AGCTTCCTTG GTGTCTTTTA 241 TCTCTATCCT TTGATGCACA CAATNNNNNN GTTGCTACTG TATTATATAA TGATCTAAGT 301 TCTTCTGATC CTGTCTGAAG GGATGGTTGT AGCTGTCCCA GTATTTGTCT ACAGCCTTCT 361 GATGTTTCTA ACAGGCCAGG ATTAACTGCG AATCGTTCTA GCTCCCTGCT TGCCCATACT 421 ATATGTTTTA ATTTATATTT TTTCTTTCCC CCTGGCCTTA ACCGAATTTT TTCCCATCGC 481 GATCTAATTC TCCCCCGCTT AATACTGACG CTCTCGCACC CATCTCTCTC CTTCTAGCCT 541 CCGCTAGTCA AAATTTTTGG CGTACTCACC AGTCGCCGCC CCTCGCCTCT TGCCGTGCGC 601 GCTTCAGCAA GCCGAGTCCT GCGTCGAGAG AGCTCCTCTG GTTTCCCTTT CGCTTTCAAG 661 TCCCTGTTCG GGCGCCACTG CTAGAGANTT TCCACACTGA CTAAAAGGGT CTGANGGNNC 721 TCTAGTTACC AGANTCACAC AACAGACGGG CACACACTAC TTGAAGCACT CAANGCAAGC 781 TTTATTGANG CTTAAGCAGN GGGTTNCCTA NTTANCCNGA NANCTCCCAN GCTCANATCT 841 GGNCTAACCA GANANANCNN NNACNNGCAA AANGCGCNGC NNATATAGAC NNCCNNNNNN 901 ANNNNCNNNN NGNCCATTNG CGNNNNNGGG GNNNNNNNNN N //
Primer: CSII-CMV5' (Pr0021) Region: insert mid Sequence file: RDB15870_A8Cdl.seq
>D05101A2-2_A8Cd_CSII-CMV5'_B01_04_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGC TGCNNCTGTC GCTGNGCTGG TCTTGGCAGT 61 GTCCGTGGTT GCCAAGGNNN NNNCCACGCT GCAGGCTAGA GTGAGCAAGG GCGAGGAGCT 121 GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT 181 CAGCGTGCGC GGCGAGGGCG AGGGCGATGC CACCATCGGC AAGCTGACCC TGAAGTTCAT 241 CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC TGACCTACGG 301 CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC 361 CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTCCTTC AAGGACGACG GCAAATACAA 421 GACCCGCGCC GTTGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG 481 CACCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA ACTTCAACAG 541 CCACAACGTC TATATCACCG CCGACAAGCA GGCTAGAGGT GGAGGCGGTT CAGGCGGAGG 601 TGGCTCTGGC GGTGGCGGAT CGGCTAGCGC CACCATGGCC CAGCTCGAAA AGGAGCTGCA 661 AGCCCTGGAG AAGGAGAACG CCCAGCTCGA ATGGGAGCTC CAGGCCCTGG AGAANGAGCT 721 GGCCCAGAAN GCTAGCGGTG GANGCGGTTC ANGCGGANGT GGCTCTGGCG GTGGCGGATC 781 GTCTAGCGGT GGANGCGGTT CANGCGGANG TGGCTCTGGC GGTGGCGGAT CGNCTAGCGG 841 TGGANGCGGN TCANGCGGAN GTGGCTCNGG CGGNGGCGGA TCGNCTANCN ANNNACTCGA 901 NAATGGCGGC NTAAGCNNGC TGGNNCANAN CNCNTCCNNN NGCTGCNGCT GCNNCTTNNC 961 NNNNNCNNNN NNNNAGCNNN NNNNNNN //
Primer: mCherry-N_R2 (Pr0518) Region: insert mid Sequence file: RDB15870_A8Cdm.seq
>D05101A2_A8Cd_2_mCherry-N_R2_A02_01_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNCNNCNAT CTNNNNNNCG TGGCCGTTCN NNGNNNCCTC 61 CNTGTGCACN NNNNNNNNNN NNNNNCTCCT TGATGATGGC CATGTTATCC TCCTCGCCCT 121 TGCTCACCAT GGTGGCTGGA TCCTTAGCGC TGGTGTACTT GGTGATGGCC TTAGTACCCT 181 CGGACACGGC GTGCTTGGCC AACTCCCCAG GCAGCAGCAG GCGCACGGCC GTCTGGATCT 241 CCCTGGAGGT GATGGTCGAG CGCTTGTTGT AATGCGCCAG GCGGGAAGCC TCACCTGCGA 301 TGCGCTCGAA AATGTCGTTC ACAAACGAAT TCATGATGCC CATGGCCTTG GACGAAATGC 361 CGGTGTCAGG GTGGACCTGC TTCAGAACCT TGTACACATA GATGGAATAG CTCTCCTTGC 421 GGCTGCGCTT GCGCTTCTTG CCGCCTTTCT TCTGCGCCTT AGTCACCGCC TTCTTGGAGC 481 CCTTTTTCGG GGCGGGAGCA GACTTCGCTG GCTCTGGCAT TGGGCCAGGA TTCTCCTCGA 541 CGTCACCGCA TGTTAGAAGA CTTCCTCTGC CCTCGCTAGA CAGAGAAATG AAGTCCAGGG 601 CTTGGAGGAG GGAGAGGGAA AGCAGCAGCA GCAGCATCCA GGATGTGTTC TGAACCAGCA 661 GGCTTATGCC GCCATTCTCG AGTTGGTAGC TAGACGATCC GCCACCGCCA GAGCCACCTC 721 CGCCTGAACC GCCTCCACCG CTAGACGATC CGCCACCGCC AGAGCCACCT CCGCCTGAAC 781 CGCCTCCANC GCTAGACGAT CCGCCACCGC CAGAGCCACC TCCGCCTGAA CCGCCTCCAC 841 CGCNAGNCTT CTGGGNCANC TCCTTCTCCN NGGCCNGGAN CTNNCATTCG AGCTNGGGCG 901 TTCTCCTTCT CCAGGGCTNG CAGCTCCNTT TTNNANCTGG GNCNNNGGTG GCNCTAGCCG 961 ATNCNNCNNN GNCANANCNN NNNNCNNNCT GNAANCGNCN TCNACCNCNA GCCNGNNNGT 1021 CGNNGGNGNN NNNNNNACGT TNNGGCNGNT GNANNTGNAN NNCCANNNTN NNGNCCCNNN 1081 ANNNNNGNNN NNNCNCCNTN NANNNNNGNN GNCCCNTNNN NCNNNNNNNN NN //
Primer: mCherry-check_R4 (Pr0527) Region: insert mid Sequence file: RDB15870_A8Cdn.seq
>15870_15870_B3D4F_mCherry-check_R4_H01_08_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NGNNCCGTNN NTGAACTGAG GGGANAGGAN GTCCCAGGCG 61 AAGGGCAGGG GGCCACCCTT GGTCACCTTC AGCTTGGCGG TCTGGGTGCC CTCGTAGGGG 121 CGGCCCTCGC CCTCGCCCTC GATCTCGAAC TCGTGGCCGT TCACGGAGCC CTCCATGTGC 181 ACCTTGAAGC GCATGAACTC CTTGATGATG GCCATGTTAT CCTCCTCGCC CTTGCTCACC 241 ATGGTGGCTG GATCCTTAGC GCTGGTGTAC TTGGTGATGG CCTTAGTACC CTCGGACACG 301 GCGTGCTTGG CCAACTCCCC AGGCAGCAGC AGGCGCACGG CCGTCTGGAT CTCCCTGGAG 361 GTGATGGTCG AGCGCTTGTT GTAATGCGCC AGGCGGGAAG CCTCACCTGC GATGCGCTCG 421 AAAATGTCGT TCACAAACGA ATTCATGATG CCCATGGCCT TGGACGAAAT GCCGGTGTCA 481 GGGTGGACCT GCTTCAGAAC CTTGTACACA TAGATGGAAT AGCTCTCCTT GCGGCTGCGC 541 TTGCGCTTCT TGCCGCCTTT CTTCTGCGCC TTAGTCACCG CCTTCTTGGA GCCCTTTTTC 601 GGGGCGGGAG CAGACTTCGC TGGCTCTGGC ATTGGGCCAG GATTCTCCTC GACGTCACCG 661 CATGTTAGAA GACTTCCTCT GCCCTCGCTA GACAGAGAAA TGAAGTCCAG GGCTTGGAGG 721 ANGGAGAGGG AAAGCAGCAG CAGCAGCATC CAGGATGTGT TCTGAACCAG CAGGCTTATG 781 CCGCCATTCT CGAGTTGGTA GCTAGACGAT CCGCCNCCGC CAGANCCACC TCCGCCTGAN 841 CCGCCTCCNC CGCTAGANNA TCCGCCACCG CCAGAGCCNC CTCCGNCNGA ACCGCCTNCN 901 CCGCTAGANG ANNCGCCNCC GCNNGANCCN CCTNCGCCTG AANCGNNTCC NCCGCTNGCC 961 NTCTGGGNCA NCTCNTTCNN NAGGGNNNNN NNCTCCCATT CNANCTGGGN NNNCNNNNNC 1021 NNCNGGGNTN GNANCTNNNT TTNNANNNNG NNNNNNNNNN //
Primer: BGH_rev2 (Pr0606) Region: 3' LTR Sequence file: RDB15870_A8Cdo.seq
>D05101A2_2_A8Cd_BGH_rev2_H01_22_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNGNNGGG CNNACNACNG ATGGCTGGCA ACTAGAANGC 61 ACAGTCGAGG CTGATCNGCG GGTTTAAACG GGCCCTGCTA GAGATTTTCC ACACTGACTA 121 AAAGGGTCTG AGGGATCTCT AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG 181 AAGCACTCAA GGCAAGCTTT ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG 241 CTCCCAGGCT CAGATCTGNN NTAACCAGAG AGACCCAGTA CAGTCCGGAT GCAGCTCTCG 301 GGCCATGTGA TGAAATGCTA GGCGGCTGTC AAACCTCCAC TCTAATACTT CTCTCTCCGG 361 GTCATCCATC CCATGCAGGC TCACAGGGTG TAACAAGCGG GTGTTCTCTC CTTCATTGGC 421 TTCTTCTACC TTCTCTTGCT CAACTGGTAC TAGCTTGTAG CACCATCCAA AGGTCAGTGG 481 ATATCTGATC CCTGGCCCTG GTGTGTAGTT CTGCCAATCA GGGAAGTAGC CTTGTGTGTG 541 GTAGATCCAC AGATCAAGGA TATCTTGTCT TCGTTGGGAG TGAATTAGCC CTTCCAGTCC 601 CCCCTTTTCT TTTAAAAAGT GGCTAAGATC TACAGCTGCC TTGTAAGTCA TTGGTCTTAA 661 AGGTACCTGA GGTGTGACTG GAAAACCCAC CTCCTCCTCC TCTTGTGCTT CTANCCNGGC 721 ACAATCAGCA TTGGTANCTG CTGTATTGCT ACTTGTGANT GCTCCATGTT TTTCTNNNTC 781 TCGACGGTAT CGATGCGGGN NNGCGGCCCN AAGGGNNNNC CGACTCNNCT GANGGCNANN 841 NNNANNANNC NNNNNANNNN NNNNANNCNN NNANCNNNNN NGGGNANNNN ANNNNNCNNC 901 NNGNANNNNN NNNNN //
Primer: SV40pro_F (Pr0110) Region: EM7 pro,BleoR Sequence file: RDB15870_A8Cdp.seq
>D05101A2_2_A8Cd_SV40pro_F_A02_02_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NANNNNTGNN GNNGNTTTTT TGGANGCCTA GGCTTTTGCN 61 AAAAGCTCCC GGGANCTTGN ATATCCATTT TCGGATCTGA TCAGCACGTG TTGACAATTA 121 ATCATCGGCA TAGTATATCG GCATAGTATA ATACGACAAG GTGAGGAACT AAACCATGGC 181 CAAGTTGACC AGTGCCGTTC CGGTGCTCAC CGCGCGCGAC GTCGCCGGAG CGGTCGAGTT 241 CTGGACCGAC CGGCTCGGGT TCTCCCGGGA CTTCGTGGAG GACGACTTCG CCGGTGTGGT 301 CCGGGACGAC GTGACCCTGT TCATCAGCGC GGTCCAGGAC CAGGTGGTGC CGGACAACAC 361 CCTGGCCTGG GTGTGGGTGC GCGGCCTGGA CGAGCTGTAC GCCGAGTGGT CGGAGGTCGT 421 GTCCACGAAC TTCCGGGACG CCTCCGGGCC GGCCATGACC GAGATCGGCG AGCAGCCGTG 481 GGGGCGGGAG TTCGCCCTGC GCGACCCGGC CGGCAACTGC GTGCACTTCG TGGCCGAGGA 541 GCAGGACTGA CACGTGCTAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG 601 CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT 661 GGAGTTCTTC GCCCACCCCN ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATANNNNNA 721 TAGCATCACA NATTTCACNA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC 781 NAAACTCATC AATGTATCTT ATCATGTCTG TATACNNTCN ANCTCTANCT AGANCTTGGC 841 GTAATCANGG NCATAGCTGT TTCCNGNNNG AAATTGNNAT NCGNNCACNN NNNNNNNCNN 901 NNNACNANCN NNNNNNNTAA NNNGTNAANN NNNNNGNNNN NNNNNN //

pCSII-CMV-MCS_n-GRAPHIC T2A H2B-mCherry

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