RDB15870 - pCSII-CMV-MCS_n-GRAPHIC T2A H2B-mCherry

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Kinoshita, N. et al., iScience 15: 28-38 (2019)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
Additional terms and conditions for distribution	The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word] This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
Remarks	Remember that you will be working with samples containing infectious virus. This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内)

[open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Kinoshita, N. et al., iScience 15: 28-38 (2019)) を引用し、謝辞の表明を必要とする。本件リソースの使用は学術機関での学術研究に限る。
付加的提供条件	レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします（Schedule AとSchedule Bを入手[link])。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

References and tips

Sequence information

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15870	pCSII-CMV-MCS_n-GRAPHIC T2A H2B-mCherry	DNA solution

Sequence (full)	RDB15870zzt01.seq compiled by us
Sequence (partial)	RDB15870pka01.seq provided by the depositor

original	Kinoshita, N., Genetically Encoded Fluorescent Indicator GRAPHIC Delineates Intercellular Connections. iScience 15: 28-38 (2019). PMID 31026667.

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check

icon as well as clone information before placing your order.

Primer: EGFP-N_R2 Region: insert 5', CMVpro_2, CMV en_2 Sequence file: RDB15870_A8Cda.seq
>D05101A2_A8Cd_2_EGFP-N_R2_E01_13_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNCN NNNNNNNNNC NCNCCNTNNG TCANGGNGGT 61 CACGANGGTG GGCCAGGGCA CGGGCAGCTT GCCGGTGGTG CAGATGAACT TCAGGGTCAG 121 CTTGCCGATG GTGGCATCGC CCTCGCCCTC GCCGCGCACG CTGAACTTGT GGCCGTTTAC 181 GTCGCCGTCC AGCTCGACCA GGATGGGCAC CACCCCGGTG AACAGCTCCT CGCCCTTGCT 241 CACTCTAGCC TGCAGCGTGG TGTTATCCTT GGCAACCACG GACACTGCCA AGACCAGCAC 301 AGCGACAGTT GGCAGCATCT CTACCATGGT GGCTCTAGCG GATCTGACGG TTCACTAAAC 361 CAGCTCTGCT TATATAGACC TCCCACCGTA CACGCCTACC GCCCATTTGC GTCAATGGGG 421 CGGAGTTGTT ACGACATTTT GGAAAGTCCC GTTGATTTTG GTGCCAAAAC AAACTCCCAT 481 TGACGTCAAT GGGGTGGAGA CTTGGAAATC CCCGTGAGTC AAACCGCTAT CCACGCCCAT 541 TGATGTACTG CCAAAACCGC ATCACCATGG TAATAGCGAT GACTAATACG TAGATGTACT 601 GCCAAGTAGG AAAGTCCCAT AAGGTCATGT ACTGGGCATA ATGCCAGGCG GGCCATTTAC 661 CGTCATTGAC GTCAATAGGG GGCGTACTTG GCATATGATA CACTTGATGT ACTGNCAAGT 721 GGGCAGTTTA CCGTAAATAC TCCACCCATT GACGTCAATG GAAAGTCCCT ATTGGCGTTA 781 CTATGGGAAC ATACGTCNTT ATTGACGTCA ATGNGCGGGG GTCGTNNGGC GGNNCAGCCA 841 GGCGGGNNAT TTANCGTAAG TTATGTAACG CGGAACTNCN TATATGGGCT ATGANCTNAT 901 GACCCCGNNA TTGATTACTA TTAATCNNAN TNNNTNCNAA GNGNATTTCT GCTGNNCCNN 961 NNNNNAANCN NAAAAANTTT NNANTTTTTG NNNNTNGNTT TNNNNNNNNN TNNNNNNNNN 1021 N //
Primer: PRE_rev Region: PRE, insert 3', Sequence file: RDB15870_A8Cdb.seq
>D05101A2_2_A8Cd_PRE_rev_G01_19_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNGNNCANC ATAGTTAAGA ATACCAGTCA ATCTTTCNCA 61 AATTTTGTAA TCCAGAGGTT GNTTATCGAT AAGCTTGATC CCTCGATGTT AACTCTAGAT 121 TACTTGTACA GCTCGTCCAT GCCGCCGGTG GAGTGGCGGC CCTCGGCGCG TTCGTACTGT 181 TCCACGATGG TGTAGTCCTC GTTGTGGGAG GTGATGTCCA ACTTGATGTT GACGTTGTAG 241 GCGCCGGGCA GCTGCACGGG CTTCTTGGCC TTGTAGGTGG TCTTGACCTC AGCGTCGTAG 301 TGGCCGCCGT CCTTCAGCTT CAGCCTCTGC TTGATCTCGC CCTTCAGGGC GCCGTCCTCG 361 GGGTACATCC GCTCGGAGGA GGCCTCCCAG CCCATGGTCT TCTTCTGCAT TACGGGGCCG 421 TCGGAGGGGA AGTTGGTGCC GCGCAGCTTC ACCTTGTAGA TGAACTCGCC GTCCTGCAGG 481 GAGGAGTCCT GGGTCACGGT CACCACGCCG CCGTCCTCGA AGTTCATCAC GCGCTCCCAC 541 TTGAAGCCCT CGGGGAAGGA CAGCTTCAAG TAGTCGGGGA TGTCGGCGGG GTGCTTCACG 601 TAGGCCTTGG AGCCGTACAT GAACTGAGGG GACAGGATGT CCCAGGCGAA GGGCAGGGGG 661 CCACCCTTGG TCACCTTCAG CTTGGCGGTC TGGGTGCCCT CGTAGGGGCG GCCCTCGCCC 721 TCGCCCTCGA TCTCGAACTC GTGGCCGTTC ACGGAGCCCT CCATGTGCAC CTTGAAGCGC 781 ATGANCTCCT TGATGATGGC CATGTTATCC TCCTCGCCCT TGCTCACCAT GGTGGCTGGA 841 TCCTTAGCGC TNNTGTACTT GGNGATGGNC TTANNANCCT CNGANACGGN GNNCTNGGNC 901 NANTNCNNNN GNANCANCNN NNNNNNNGNN NNNNTGGNNN NNCCNNGNNN NNNNNNGNNN 961 NNNNNNN //
Primer: CSII-CMV5' Region: insert mid Sequence file: RDB15870_A8Cdc.seq
>D05101A2-2_A8Cd_CSII-CMV5'_B01_04_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGC TGCNNCTGTC GCTGNGCTGG TCTTGGCAGT 61 GTCCGTGGTT GCCAAGGNNN NNNCCACGCT GCAGGCTAGA GTGAGCAAGG GCGAGGAGCT 121 GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT 181 CAGCGTGCGC GGCGAGGGCG AGGGCGATGC CACCATCGGC AAGCTGACCC TGAAGTTCAT 241 CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC TGACCTACGG 301 CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC 361 CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTCCTTC AAGGACGACG GCAAATACAA 421 GACCCGCGCC GTTGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG 481 CACCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA ACTTCAACAG 541 CCACAACGTC TATATCACCG CCGACAAGCA GGCTAGAGGT GGAGGCGGTT CAGGCGGAGG 601 TGGCTCTGGC GGTGGCGGAT CGGCTAGCGC CACCATGGCC CAGCTCGAAA AGGAGCTGCA 661 AGCCCTGGAG AAGGAGAACG CCCAGCTCGA ATGGGAGCTC CAGGCCCTGG AGAANGAGCT 721 GGCCCAGAAN GCTAGCGGTG GANGCGGTTC ANGCGGANGT GGCTCTGGCG GTGGCGGATC 781 GTCTAGCGGT GGANGCGGTT CANGCGGANG TGGCTCTGGC GGTGGCGGAT CGNCTAGCGG 841 TGGANGCGGN TCANGCGGAN GTGGCTCNGG CGGNGGCGGA TCGNCTANCN ANNNACTCGA 901 NAATGGCGGC NTAAGCNNGC TGGNNCANAN CNCNTCCNNN NGCTGCNGCT GCNNCTTNNC 961 NNNNNCNNNN NNNNAGCNNN NNNNNNN //
Primer: mCherry-N_rev Region: insert mid Sequence file: RDB15870_A8Cdd.seq
>D05101A2-2_A8Cd_mCherry_N_rev_C01_07_ABI24.ab1 1 NNNNNNNNNN NNNNNNNGNN GGNNNTGTTA TCCTCCTCGC CCTTGCTCNC CATGGTGGCT 61 GGATCCTTAG CGCTNGTGTA CTTGGTGATG GCCTTAGTAC CCTCGGACAC GGCGTGCTTG 121 GCCAACTCCC CAGGCAGCAG CAGGCGCACG GCCGTCTGGA TCTCCCTGGA GGTGATGGTC 181 GAGCGCTTGT TGTAATGCGC CAGGCGGGAA GCCTCACCTG CGATGCGCTC GAAAATGTCG 241 TTCACAAACG AATTCATGAT GCCCATGGCC TTGGACGAAA TGCCGGTGTC AGGGTGGACC 301 TGCTTCAGAA CCTTGTACAC ATAGATGGAA TAGCTCTCCT TGCGGCTGCG CTTGCGCTTC 361 TTGCCGCCTT TCTTCTGCGC CTTAGTCACC GCCTTCTTGG AGCCCTTTTT CGGGGCGGGA 421 GCAGACTTCG CTGGCTCTGG CATTGGGCCA GGATTCTCCT CGACGTCACC GCATGTTAGA 481 AGACTTCCTC TGCCCTCGCT AGACAGAGAA ATGAAGTCCA GGGCTTGGAG GAGGGAGAGG 541 GAAAGCAGCA GCAGCAGCAT CCAGGATGTG TTCTGAACCA GCAGGCTTAT GCCGCCATTC 601 TCGAGTTGGT AGCTAGACGA TCCGCCACCG CCAGAGCCAC CTCCGCCTGA ACCGCCTCCA 661 CCGCTAGACG ATCCGCCACC GCCAGAGCCA CCTCCGCCTG AACCGCCTCC ACCGCTAGAC 721 GATCCGCCAC CGCCAGANCC ACCTCCGCCT GAACCGCCTC CACCGCTAGC CTTCTGGGCC 781 AGCTCCTNCT CCAGGGCCTG GAGCTCCNAT TCGAGCTGGG CGTTCTCCTT CTCCAGGGCT 841 NNCAGCTCCT TTTCGANCTG GGCCATGGNG GCGCTANCCG ATNCGCCACC GNCAGANCCA 901 CCTNCGCCNN AACCGCNNNC ANCNCTANCC NGCTNNNNGG NGGNNNNATA GNNNNNNNNG 961 NNNNNNAAGN TNGNANNNNN NNNNN //
Primer: HIVgp_R1 Region: HIV psi, 5'LTR Sequence file: RDB15870_A8Cde.seq
>D05101A2_2_A8Cd_HIVgp-R1_B02_05_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNC NCTTTTTTCT CTCTGCACCN CTCTTCTCTT TGCCTTGGTG 61 GGTGCTACTC CTAATGGTTC NATTTTTACT ACTTTATATT TATATAATTC ACTTCTCCAA 121 TTGTCCCTCA TATCTCCTCC TCCAGGTCTG AAGATCAGCG GCCGGCCGCT TGCTGTGCGG 181 TGGTCTTACT TTTGTTTTGC TCTTCCTCTA TCTTGTCTAA AGCTTCCTTG GTGTCTTTTA 241 TCTCTATCCT TTGATGCACA CAATNNNNNN GTTGCTACTG TATTATATAA TGATCTAAGT 301 TCTTCTGATC CTGTCTGAAG GGATGGTTGT AGCTGTCCCA GTATTTGTCT ACAGCCTTCT 361 GATGTTTCTA ACAGGCCAGG ATTAACTGCG AATCGTTCTA GCTCCCTGCT TGCCCATACT 421 ATATGTTTTA ATTTATATTT TTTCTTTCCC CCTGGCCTTA ACCGAATTTT TTCCCATCGC 481 GATCTAATTC TCCCCCGCTT AATACTGACG CTCTCGCACC CATCTCTCTC CTTCTAGCCT 541 CCGCTAGTCA AAATTTTTGG CGTACTCACC AGTCGCCGCC CCTCGCCTCT TGCCGTGCGC 601 GCTTCAGCAA GCCGAGTCCT GCGTCGAGAG AGCTCCTCTG GTTTCCCTTT CGCTTTCAAG 661 TCCCTGTTCG GGCGCCACTG CTAGAGANTT TCCACACTGA CTAAAAGGGT CTGANGGNNC 721 TCTAGTTACC AGANTCACAC AACAGACGGG CACACACTAC TTGAAGCACT CAANGCAAGC 781 TTTATTGANG CTTAAGCAGN GGGTTNCCTA NTTANCCNGA NANCTCCCAN GCTCANATCT 841 GGNCTAACCA GANANANCNN NNACNNGCAA AANGCGCNGC NNATATAGAC NNCCNNNNNN 901 ANNNNCNNNN NGNCCATTNG CGNNNNNGGG GNNNNNNNNN N //
Primer: BGH_rev2 Region: 3'LTR Sequence file: RDB15870_A8Cdf.seq
>D05101A2_2_A8Cd_BGH_rev2_H01_22_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNGNNGGG CNNACNACNG ATGGCTGGCA ACTAGAANGC 61 ACAGTCGAGG CTGATCNGCG GGTTTAAACG GGCCCTGCTA GAGATTTTCC ACACTGACTA 121 AAAGGGTCTG AGGGATCTCT AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG 181 AAGCACTCAA GGCAAGCTTT ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG 241 CTCCCAGGCT CAGATCTGNN NTAACCAGAG AGACCCAGTA CAGTCCGGAT GCAGCTCTCG 301 GGCCATGTGA TGAAATGCTA GGCGGCTGTC AAACCTCCAC TCTAATACTT CTCTCTCCGG 361 GTCATCCATC CCATGCAGGC TCACAGGGTG TAACAAGCGG GTGTTCTCTC CTTCATTGGC 421 TTCTTCTACC TTCTCTTGCT CAACTGGTAC TAGCTTGTAG CACCATCCAA AGGTCAGTGG 481 ATATCTGATC CCTGGCCCTG GTGTGTAGTT CTGCCAATCA GGGAAGTAGC CTTGTGTGTG 541 GTAGATCCAC AGATCAAGGA TATCTTGTCT TCGTTGGGAG TGAATTAGCC CTTCCAGTCC 601 CCCCTTTTCT TTTAAAAAGT GGCTAAGATC TACAGCTGCC TTGTAAGTCA TTGGTCTTAA 661 AGGTACCTGA GGTGTGACTG GAAAACCCAC CTCCTCCTCC TCTTGTGCTT CTANCCNGGC 721 ACAATCAGCA TTGGTANCTG CTGTATTGCT ACTTGTGANT GCTCCATGTT TTTCTNNNTC 781 TCGACGGTAT CGATGCGGGN NNGCGGCCCN AAGGGNNNNC CGACTCNNCT GANGGCNANN 841 NNNANNANNC NNNNNANNNN NNNNANNCNN NNANCNNNNN NGGGNANNNN ANNNNNCNNC 901 NNGNANNNNN NNNNN //
Primer: SV40pro_F Region: EM7 pro, BleoR Sequence file: RDB15870_A8Cdg.seq
>D05101A2_2_A8Cd_SV40pro_F_A02_02_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NANNNNTGNN GNNGNTTTTT TGGANGCCTA GGCTTTTGCN 61 AAAAGCTCCC GGGANCTTGN ATATCCATTT TCGGATCTGA TCAGCACGTG TTGACAATTA 121 ATCATCGGCA TAGTATATCG GCATAGTATA ATACGACAAG GTGAGGAACT AAACCATGGC 181 CAAGTTGACC AGTGCCGTTC CGGTGCTCAC CGCGCGCGAC GTCGCCGGAG CGGTCGAGTT 241 CTGGACCGAC CGGCTCGGGT TCTCCCGGGA CTTCGTGGAG GACGACTTCG CCGGTGTGGT 301 CCGGGACGAC GTGACCCTGT TCATCAGCGC GGTCCAGGAC CAGGTGGTGC CGGACAACAC 361 CCTGGCCTGG GTGTGGGTGC GCGGCCTGGA CGAGCTGTAC GCCGAGTGGT CGGAGGTCGT 421 GTCCACGAAC TTCCGGGACG CCTCCGGGCC GGCCATGACC GAGATCGGCG AGCAGCCGTG 481 GGGGCGGGAG TTCGCCCTGC GCGACCCGGC CGGCAACTGC GTGCACTTCG TGGCCGAGGA 541 GCAGGACTGA CACGTGCTAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG 601 CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT 661 GGAGTTCTTC GCCCACCCCN ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATANNNNNA 721 TAGCATCACA NATTTCACNA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC 781 NAAACTCATC AATGTATCTT ATCATGTCTG TATACNNTCN ANCTCTANCT AGANCTTGGC 841 GTAATCANGG NCATAGCTGT TTCCNGNNNG AAATTGNNAT NCGNNCACNN NNNNNNNCNN 901 NNNACNANCN NNNNNNNTAA NNNGTNAANN NNNNNGNNNN NNNNNN //

pCSII-CMV-MCS_n-GRAPHIC T2A H2B-mCherry

Distribution information

References and tips

Sequence information

References