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pCSII-CMV-MCS _n-GRAPHIC

Lentiviral expression vector of GRAPHIC probe (LZA).

Catalog number RDB15869
Resource name pCSII-CMV-MCS _n-GRAPHIC
Clone info. Lentiviral expression vector of GRAPHIC probe (GPI anchored Reconstitution-Activated Proteins Highlight Intercellular Connections), fluorescent indicator for intercellular contacts with optimized intercellular GFP reconstitution. This probe contains an mouse preproacrosin signal peptide (SP), superfolder GFP-NT, acidic leucine zipper domain (LZA), mouse Thy-1 GPI-anchored domain, see Fig. 1 of Kinoshita, N. et al., iScience 15: 28-38, 2019.
Expression was confirmed by the depositor with detection of reconstituted GFP fluorescence. Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-CMV-MCS (plasmid)
Size of vector backbone 8.6 kb
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Gene/insert name mouse Acr cDNA mouse Thy1 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Shimogori, Tomomi | Kinoshita, Nagatoki |
 
Other clones in our bank mouse Acr (NCBI Gene 11434) | mouse Thy1 (NCBI Gene 21838) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Kinoshita, N. et al., iScience 15: 28-38 (2019)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
Additional terms and conditions for distribution The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
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Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Schedule A (GFP-A) [Link]
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB15869 pCSII-CMV-MCS _n-GRAPHIC Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Sequence (partial) RDB15869pka01.seq provided by the depositor

Original reference

original Kinoshita, N., Genetically Encoded Fluorescent Indicator GRAPHIC Delineates Intercellular Connections. iScience 15: 28-38 (2019). PMID 31026667.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information


References

Original, user report and related articles

original Kinoshita, N., Genetically Encoded Fluorescent Indicator GRAPHIC Delineates Intercellular Connections. iScience 15: 28-38 (2019). PMID 31026667.

2021.08.14

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