Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCSII-CMV-MCS _n-GRAPHIC

Lentiviral expression vector of GRAPHIC probe (LZA).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Kinoshita, N. et al., iScience 15: 28-38 (2019)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. Additional terms and conditions: The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Remarks	Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Kinoshita, N. et al., iScience 15: 28-38 (2019)) を引用し、謝辞の表明を必要とする。本件リソースの使用は学術機関での学術研究に限る。 付加的提供条件： レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15869_A8Cdp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-N_R2 (Pr0905) Region: insert 5', CMV pro_2 Sequence file: RDB15869_A8Cda.seq
>D05101A1_A8Cd_1_EGFP-N_R2_C01_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNCNNN NNCNNNNNNC GCCNNANGTC NNGGNGGTCA    61 CGNNGGTGGG CCAGGGCACG GGCAGCTTGC CGGTGGTGCA GATGAACTTC AGGGTCAGCT   121 TGCCGATGGT GGCATCGCCC TCGCCCTCGC CGCGCACGCT GAACTTGTGG CCGTTTACGT   181 CGCCGTCCAG CTCGACCAGG ATGGGCACCA CCCCGGTGAA CAGCTCCTCG CCCTTGCTCA   241 CTCTAGCCTG CAGCGTGGTG TTATCCTTGG CAACCACGGA CACTGCCAAG ACCAGCACAG   301 CGACAGTTGG CAGCATCTCT ACCATGGTGG CTCTAGCGGA TCTGACGGTT CACTAAACCA   361 GCTCTGCTTA TATAGACCTC CCACCGTACA CGCCTACCGC CCATTTGCGT CAATGGGGCG   421 GAGTTGTTAC GACATTTTGG AAAGTCCCGT TGATTTTGGT GCCAAAACAA ACTCCCATTG   481 ACGTCAATGG GGTGGAGACT TGGAAATCCC CGTGAGTCAA ACCGCTATCC ACGCCCATTG   541 ATGTACTGCC AAAACCGCAT CACCATGGTA ATAGCGATGA CTAATACGTA GATGTACTGC   601 CAAGTAGGAA AGTCCCATAA GGTCATGTAC TGGGCATAAT GCCAGGCGGG CCATTTACCG   661 TCATTGACGT CAATAGGGGG CGTANTTGGC ATATGATACA CTTGATGTAN TGCCAAGTGG   721 GCAGTTTACC GTAAATACTC CACCCATTGA CGTCNATGGA AAGTCCCTAT TGNCGTTACT   781 ATGGGAACAT ACGTCATTAT TGACGTCANT GGGCGGGGGT CGTTGGGCNG NCANNCNNGC   841 NGGCCATTTA NCGTAAGTTA TGTNACGCNN ANNTNCATAT ATGGGCTATG ANNTNATGAN 901 CCCGTNNTTG NNNNNNNNTN NNNNNNNNN //
Primer: PRE_rev (Pr0250) Region: WPRE, insert 3' Sequence file: RDB15869_A8Cdb.seq
>D05101A1_1_A8Cd_PRE_rev_B01_04_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNGNNCNNN NTAGTTAAGA NTACNNGTCA ATCTTTCNCA    61 AATTTTGTAA TCCAGAGGTT GNTTATCGAT AAGCTTGATC CCTCGATGTT AACTCTAGAG   121 GATCCGCGGC CGCTGCAGAA TTCTCGAGAC CGGTAGCGCT AGATTACAGA GAAATGAAGT   181 CCAGGGCTTG GAGGAGGGAG AGGGAAAGCA GCAGCAGCAG CATCCAGGAT GTGTTCTGAA   241 CCAGCAGGCT TATGCCGCCA TTCTCGAGTT GGTAGCTAGA CGATCCGCCA CCGCCAGAGC   301 CACCTCCGCC TGAACCGCCT CCACCGCTAG ACGATCCGCC ACCGCCAGAG CCACCTCCGC   361 CTGAACCGCC TCCACCGCTA GACGATCCGC CACCGCCAGA GCCACCTCCG CCTGAACCGC   421 CTCCACCGCT AGCCTTCTGG GCCAGCTCCT TCTCCAGGGC CTGGAGCTCC CATTCGAGCT   481 GGGCGTTCTC CTTCTCCAGG GCTTGCAGCT CCTTTTCGAG CTGGGCCATG GTGGCGCTAG   541 CCGATCCGCC ACCGCCAGAG CCACCTCCGC CTGAACCGCC TCCACCGGAT CTCTGCTTGT   601 CGGCGGTGAT ATAGACGTTG TGGCTGTTGA AGTTGTACTC CAGCTTGTGC CCCAGGATGT   661 TGCCGTCCTC CTTGAAGTCG GTGCCCTTCA GCTCGATGCG GTTCACCAGG GTGTCGCCCT   721 CNAACTTCAC AACGGCGCGG GTCTTGTATT TGCCGTCGTC CTTGANNGAN ANGGTGCGCT   781 NCTGGANGTA NCCNTCNGGC ATGGNGGANT NNAAGAANTC NNGCTGCTTC ATGTGGNCNG   841 GGGTANCGGN NGNNNNNCTG CNCNCNNNNN NNCNNGNNGG NCACNANGGN GGGNNNNGGG 901 CACGNNNGCT NGNCNNNNNN NNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori, BleoR Sequence file: RDB15869_A8Cdc.seq
>D05101A1_A8Cd_1_SV40pro_ori_F_D01_10_ABI24.ab1     1 NNNNNNNNNN NNCNNNNNNC GCCTCTGCCT CTGANCTATT CNNGAAGTAG TGAGGANGCT    61 TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT   121 CTGATCAGCA CGTGTTGACA ATTAATCATC GGCATAGTAT ATCGGCATAG TATAATACGA   181 CAAGGTGAGG AACTAAACCA TGGCCAAGTT GACCAGTGCC GTTCCGGTGC TCACCGCGCG   241 CGACGTCGCC GGAGCGGTCG AGTTCTGGAC CGACCGGCTC GGGTTCTCCC GGGACTTCGT   301 GGAGGACGAC TTCGCCGGTG TGGTCCGGGA CGACGTGACC CTGTTCATCA GCGCGGTCCA   361 GGACCAGGTG GTGCCGGACA ACACCCTGGC CTGGGTGTGG GTGCGCGGCC TGGACGAGCT   421 GTACGCCGAG TGGTCGGAGG TCGTGTCCAC GAACTTCCGG GACGCCTCCG GGCCGGCCAT   481 GACCGAGATC GGCGAGCAGC CGTGGGGGCG GGAGTTCGCC CTGCGCGACC CGGCCGGCAA   541 CTGCGTGCAC TTCGTGGCCG AGGAGCANGA CTGACACGTG CTACGAGATT TCGATTCCAC   601 CGCCGCCTTC TATGAAAGGN TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT   661 CCTCNNGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCNC CCCAACTTGN TTATTGCANC   721 TTATAATGGT TACAAATAAA GCAATANCAT CACNAATTTC NCAAATAANN CNNTTTTTTT   781 CNNTGCATTC TANTTGTGGT TTGNNCNAAA CTCNTCANTG TATCTTATCN TGTCTGNATA   841 NCGTCNANCT CTANCTAGAN CTNGNNNNAN TCATGGNCNT AGCTGTTNNC NGNGNNAAAT   901 NNNTANNCGC TCNNANTNCC NCNCNNCATA NNNNNCNNNA ANNNTNAANN NNNNNNNCCN 961 NNNGNNGNCN NNNNGNNNNN NCNNNNNNNN NN //
Primer: HIV-B_R (Pr0673) Region: HIV-1 psi, 5'LTR, CMV pro_1 Sequence file: RDB15869_A8Cdd.seq
>D05101A1_A8Cd_1_HIV-B_R_A01_01_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNANNT TTTGNNNNNN NCNNNNNNCG CCGCCCCTCG    61 CCTCTTGCCG TGCGCGCTNC NNCAAGCCGA GTCCTGCGTC GAGAGAGCTC CTCTGGTTTC   121 CCTTTCGCTT TCAAGTCCCT GTTCGGGCGC CACTGCTAGA GATTTTCCAC ACTGACTAAA   181 AGGGTCTGAG GGATCTCTAG TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA   241 GCACTCAAGG CAAGCTTTAT TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT   301 CCCAGGCTCA GATCTGGTCT AACCAGAGAG ACCCAGTACA GGCAAAACGC GCTGCTTATA   361 TAGACCTCCC ACCGTACACG CCTACCGCCC ATTTGCGTCA ATGGGGCGGA GTTGTTACGA   421 CATTTTGGAA AGTCCCGTTG ATTTTGGTGC CAAAACAAAC TCCCATTGAC GTCAATGGGG   481 TGGAGACTTG GAAATCCCCG TGAGTCAAAC CGCTATCCAC GCCCATTGAT GTACTGCCAA   541 AACCGCATCA CCATGGTAAT AGCGATGACT AATACGTAGA TGTACTGCCA AGTAGGAAAG   601 TCCCATNAGG TCATGTACTG NNNNNNATGC CAGGCGGGCC ATTTACCGTC ATTGACGTCN   661 ATAGGGGGCG TACTTGGCAT ATGATACACT TGANGTACTG NCNAGTGGGC AGNTTTANCG   721 TAAATACTCC ACCCATTGAC GTCAATGGAA AAGNNCCTAT TGGCGTTACT ATGGGAACAT   781 ANGTCNTTAT TGANNTCANT GGGNGGGGGT CGTTNGGCGG TCAGCCNGGC GGGNCNTTTA   841 NCGTANNNNA TGTAACGNNG NNANNTCNNN ATNTGGGNCT ATGANNNNNN NGNNCCCCGT   901 AATTNNATTN NNANTNANTN ANNNANNNNN ANNANNNNNN GNNCNANNNC GNANNNTCNN 961 GGNCCCNNNA NANNCNNNNA ANNNNCNGNN NAANNGNNNN NNNNCCCNNN NNNNNNN //
Primer: RRE-M_R (Pr0651) Region: RRE Sequence file: RDB15869_A8Cde.seq
>D05101A1_A8Cd_1_RRE-M_R_B01_04_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTGN GCCTCNNAGC CCTCAGCAAA TTGTTCTGCT    61 GCTGCACTAT ACCAGNCAAT AATTGTCTGG CCTGTACCGT CAGCGTCATT GACGCTGCGC   121 CCATAGTGCT TCCTGCTGCT CCCAAGAACC CAAGGAACAA AGCTCCTATT CCCACTGCTC   181 TTTTTTCTCT CTGCACCACT CTTCTCTTTG CCTTGGTGGG TGCTACTCCT AATGGTTCAA   241 TTTTTACTAC TTTATATTTA TATAATTCAC TTCTCCAATT GTCCCTCATA TCTCCTCCTC   301 CAGGTCTGAA GATCAGCGGC CGGCCGCTTG CTGTGCGGTG GTCTTACTTT TGTTTTGCTC   361 TTCCTCTATC TTGTCTAAAG CTTCCTTGGT GTCTTTTATC TCTATCCTTT GATGCACACA   421 ATAGAGGGTT GCTACTGTAT TATATAATGA TCTAAGTTCT TCTGATCCTG TCTGAAGGGA   481 TGGTTGTAGC TGTCCCAGTA TTTGTCTACA GCCTTCTGAT GTTTCTAACA GGCCAGGATT   541 AACTGCGAAT CGTTCTAGCT CCCTGCTTGC CCATACTATA TGTTTTAATT TATATTTTTT   601 CTTTCCCCCT GGCCTTAACC GAATTTTTTC CCATCNNNNN CTAATTCTCC CCCGCTTAAT   661 ACTGACGCTC TCGCACCCAT CTCTCTCCTT CTAGCCTCCG CTAGTCAAAA TTTTTGGCGT   721 ACTCACCAGN CGCCGCCCCT CGCCTCTTNN NGTGCGCGCT TCANCAAGCC NAGTCCTGCG   781 TCNNGAGAGC TCCTCTGGNT NCCCTTTCGC TTNNAAGTNC CTGTTCNGGC GCCNCTGCTA   841 NANANTTTCC ACACNGANNN AAANGGNCTG AGGGANCTCT AGTNANCNNN ANTCNCNNNN   901 NANANGGGCN NNNNCNACNT GNANCANNCN ANGGNNNGCT TNANNGNGNN TTNNNNNGNG 961 GGNNNNCNTA NNNNNCNNNN NNNNNN //
Primer: RRE_F1 (Pr0796) Region: CPPT_CTS Sequence file: RDB15869_A8Cdf.seq
>D05101A1_A8Cd_1_RRE_F_B01_02_ABI08.ab1     1 TTTGGGGTTG CTCTGGAAAA CTCATTTGCA CCACTGCTGT GCCTNGNNAT GCTAGTTGGA    61 GTAATAAATC TCTGGAACAG ATTTGGAATC ACACGACCTG GATGGAGTGG GACAGAGAAA   121 TTAACAATTA CACAAGCTTA ATACACTCCT TAATTGAAGA ATCGCAAAAC CAGCAAGAAA   181 AGAATGAACA AGAATTATTG GAATTAGATA AATGGGCAAG TTTGTGGAAT TGGTTTAACA   241 TAACAAATTG GCTGTGGTAT ATAAAATTAT TCATAATGAT AGTAGGAGGC TTGGTAGGTT   301 TAAGAATAGT TTTTGCTGTA CTTTCTATAG TGAATAGAGT TAGGCAGGGA TATTCACCAT   361 TATCGTTTCA GACCCACCTC CCAACCCCGA GGGGACCCGA CAGGCCCGAA GGAATAGAAG   421 AAGAAGGTGG AGAGAGAGAC AGAGACAGAT CCATTCGATT AGTGAACGGA TCTACAAATG   481 GCAGTATTCA TCCACAATTT TAAAAGAAAA GGGGGGATTG GGGGGTACAG TGCAGGGGAA   541 AGAATAGTAG ACATAATAGC AACAGACNTA CAAACTAAAG AATTACAAAA ACNAANTTAC   601 AAAAATTCAA AATTTTCGGG TTTATTACAG GGGACAGCAN AAANTTCACT TTGAATTAAT   661 TCGATTAATA GTAATCAATT NNNGGGGTCA TTANTTCATA GCCCATATAT GGAGTTCCGC   721 GTTACNNAAC TTACGGTAAA TGGCCCGCNT GGCTGANCGC CCAACGACCC CNNCCCATTG   781 NNGTCNANNA NGACNNNNGT TNCNNNNGNN NCGNNNNNNN GGANTTTNCA NNNACGNNNN 841 NGGGNGGNNN ANTNNNNGNA ANNNNCNNNN NNNNNN //
Primer: BGH_rev2 (Pr0606) Region: 3' LTR Sequence file: RDB15869_A8Cdg.seq
>D05101A1_1_A8Cd_BGH_rev2_C01_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNGGNNGGGC NNNCAACAGN TGGCTGGCAA CTAGAAGGCA    61 CAGTCGAGGC NNNNNNNNNN NNNNAAACGG GCCCTGCTAG AGATTTTCCA CACTNNCTAA   121 AAGGGTCTGA GGGATCTCTA GTTACCAGAG TCACACAACA GACGGGCACA CACTACTTGA   181 AGCACTCAAG GCAAGCTTTA TTGAGGCTTA AGCAGTGGGT TCCCTAGTTA GCCAGAGAGC   241 TCCCAGGCTC AGATCTGGTC TAACCAGAGA GACCCAGTAC AGTCCGGATG CAGCTCTCGG   301 GCCATGTGAT GAAATGCTAG GCGGCTGTCA AACCTCCACT CTAATACTTC TCTCTCCGGG   361 TCATCCATCC CATGCAGGCT CACAGGGTGT AACAAGCGGG TGTTCTCTCC TTCATTGGCT   421 TCTTCTACCT TCTCTTGCTC AACTGGTACT AGCTTGTAGC ACCATCCAAA GGTCAGTGGA   481 TATCTGATCC CTGGCCCTGG TGTGTAGTTC TGCCAATCAG GGAAGTAGCC TTGTGTGTGG   541 TAGATCCACA GATCAAGGAT ATCTTGTCTT CGTTGGGAGT GAATTAGCCC TTCCAGTCCC   601 CCCTTTTCTT TTAAAAAGTG GCTAAGATCT ACAGCTGCCT TGTAAGTCAT TGGTCTTAAA   661 GGTACCTGAN GTGTGACTGG AAAACCCACC TCCTCCTCCT CTTGTGCTTC TAGCCAGGCA   721 CAATCAGCAT TGNTAGCTGC TGTATTGCTA CTTGTGATTG CTCCATGTTT TTCTAGGTCT   781 CGACNGTATC GATGCGGGGN NGCGGCCNNA AGGGNNNTCC GANTNGNCTG ANGNCGAANN   841 NNNAAGANNC NGNANANGCC GCNNANCNNN NANCNNNCCN CNGGNNNNNA NNNNNCNCNN 901 NNNTNNNNNN CNNNNGNNNN TNNNANNNNN NNNTNNNNNN NNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl