Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGEX-6p-2-3MST (#RDB15868)

E. coli expression vector of mouse 3-mercaptopyruvate sulfurtransferase (3MST).

Clone info. E. coli expression vector of mouse 3-mercaptopyruvate sulfurtransferase (3MST, Mpst) fused with GST at N-terminal.
Comment Note: nucleotide substitution in mouse Mpst (A to G in 499nt of NM_138670.3 (Asp to Gly)), which has been noticed by the depositor.
Vector backbone pGEX-6P-2 (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name Mouse Mpst cDNA
Depositor|Developer Kimura, Hideo |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature designated by the DEPOSITOR are requested (Kimura, Y et al. Sci. Rep. 7(1), 10459, 2017). Any commercial use of the BIOLOGICAL RESOURCE requires prior written consent of the DEPOSITOR.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Kimura, Y et al. Sci Rep. 7(1), 10459, 2017)を引用し、謝辞の表明を必要とする。営利機関の利用者もしくは商業利用を目的とする利用者は、事前にその旨を寄託者に届出し、また提供承諾書を取得することとする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15868 pGEX-6p-2-3MST DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGEX-6p-2-3MST was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15868).

Reference section:

Kimura, Y., Koike, S., Shibuya, N., Lefer, D., Ogasawara, Y., Kimura, H., 3-Mercaptopyruvate sulfurtransferase produces potential redox regulators cysteine- and glutathione-persulfide (Cys-SSH and GSSH) together with signaling molecules H2S2, H2S3 and H2S. Sci. Rep. 7 (1): 10459 (2017). PMID 28874874. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15868_A8Cbp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX-F2
Sequence file: RDB15868_A8Cba.seq check
1
Primer: pGEX3'
Sequence file: RDB15868_A8Cbb.seq check
1
Primer: SV40pA3' element Reverse
Sequence file: RDB15868_A8Cbc.seq check
1
Primer: pGEX-ptac_up-F
Sequence file: RDB15868_A8Cbd.seq check
1

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kimura, Y., 3-Mercaptopyruvate sulfurtransferase produces potential redox regulators cysteine- and glutathione-persulfide (Cys-SSH and GSSH) together with signaling molecules H2S2, H2S3 and H2S. Sci. Rep. 7 (1): 10459 (2017). PMID 28874874. [link to RRC of NBRP]