Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGEX-6p-2-3MST

E. coli expression vector of mouse 3-mercaptopyruvate sulfurtransferase (3MST).

Catalog number RDB15868
Resource name pGEX-6p-2-3MST
Clone info. E. coli expression vector of mouse 3-mercaptopyruvate sulfurtransferase (3MST, Mpst) fused with GST at N-terminal.
Note: nucleotide substitution in mouse Mpst (A to G in 499nt of NM_138670.3 (Asp to Gly)), which has been noticed by the depositor.
Vector backbone pGEX-6P-2 (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name Mouse Mpst cDNA
Depositor|Developer Kimura, Hideo |
 
Other clones in our bank mouse Mpst (NCBI Gene 246221) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature designated by the DEPOSITOR are requested (Kimura, Y et al. Sci. Rep. 7(1), 10459, 2017). Any commercial use of the BIOLOGICAL RESOURCE requires prior written consent of the DEPOSITOR.
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15868 pGEX-6p-2-3MST DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Kimura, Y., 3-Mercaptopyruvate sulfurtransferase produces potential redox regulators cysteine- and glutathione-persulfide (Cys-SSH and GSSH) together with signaling molecules H2S2, H2S3 and H2S. Sci. Rep. 7 (1): 10459 (2017). PMID 28874874.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15868_A8Cbp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX-F2
Sequence file: RDB15868_A8Cba.seq check
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Primer: pGEX3'
Sequence file: RDB15868_A8Cbb.seq check
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Primer: SV40pA3' element Reverse
Sequence file: RDB15868_A8Cbc.seq check
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Primer: pGEX-ptac_up-F
Sequence file: RDB15868_A8Cbd.seq check
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Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kimura, Y., 3-Mercaptopyruvate sulfurtransferase produces potential redox regulators cysteine- and glutathione-persulfide (Cys-SSH and GSSH) together with signaling molecules H2S2, H2S3 and H2S. Sci. Rep. 7 (1): 10459 (2017). PMID 28874874.

2021.03.25

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