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pcDNA-DEST47-TOP2A

Gateway(R) expression vector of human TOP2A.

Catalog number RDB15801
Resource name pcDNA-DEST47-TOP2A
Clone info. Gateway(R) expression vector of human topoisomerase 2 alpha (TOP2A) fused with GFP at C-terminal, CMV promoter.
Vector backbone pcDNA-DEST47 (plasmid)
Size of vector backbone 7.8 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human TOP2A cDNA
Depositor|Developer Kondo, Yutaka |
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External Database
human TOP2A

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Katsushima, K et al. Nat. Commun. 7: 13616, 2016); (Deguchi, S et al. Oncogene. 36 (32) : 4629 - 4640, 2017) RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Schedule A (GFP-A) [Link]
          Approval form by depositor [Word]
          Remarks ((Additional form)) Approval Form(FormD)
          Please use Schedule A (GFP-A) additionally.
          Please obtain written approval of the depositor.
          Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
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          RDB15801 pcDNA-DEST47-TOP2A DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Deguchi, S., Oncogenic effects of evolutionarily conserved noncoding RNA ECONEXIN on gliomagenesis. Oncogene 36 (32): 4629-4640 (2017). PMID 28368417.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15801_A8Bqp1-3.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro,attB1,insert 5'
          Sequence file: RDB15801_A8Bqe.seq check
          >D05196A8-6_A8Bq_CMV_Forward_E02_2018-03-19-14-24-28_14.ab1
              1 NNNNNNNNNN NNNCNTNGNN GCNNTGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
             61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
            121 CTCACTATAG GGAGACCCAA GCTGGCTAGT TAAGCTTGAT CAAACAAGTT TGTACAAAAA
            181 AGCAGGCTCC GCGGCCGCCC CCTTCACCAT GGAAGTGTCA CCATTGCAGC CTGTAAATGA
            241 AAATATGCAA GTCAACAAAA TAAAGAAAAA TGAAGATGCT AAGAAAAGAC TGTCTGTTGA
            301 AAGAATCTAT CAAAAGAAAA CACAATTGGA ACATATTTTG CTCCGCCCAG ACACCTACAT
            361 TGGTTCTGTG GAATTAGTGA CCCAGCAAAT GTGGGTTTAC GATGAAGATG TTGGCATTAA
            421 CTATAGGGAA GTCACTTTTG TTCCTGGTTT GTACAAAATC TTTGATGAGA TTCTAGTTAA
            481 TGCTGCGGAC AACAAACAAA GGGACCCAAA AATGTCTTGT ATTAGAGTCA CAATTGATCC
            541 GGAAAACAAT TTAATTAGTA TATGGAATAA TGGAAAAGGT ATTCCTGTTG TTGAACACAA
            601 AGTTGAAAAG ATGTATGTCC CAGCTCTCAT ATTTGGACAG CTCCTAACTT CTAGTAACTA
            661 TGATGATGAT GAAAAGAAAG TGACAGGTGG TCGAAATGGC TATGGAGCCA AATTGTGTAA
            721 CATATTCAGT ACCNAATTTA CTGTGGAAAC AGCCAGTAGA GAATACAAGA AAATGTTCNA
            781 ACAGACNTGG NTGGATAATA TGGGAAGANC TGGTGAGATG NAACTCNAGC CNNNCNNNGG
            841 NNAAGANTAT ACATGTATCA CCTTTCANCC NNNNTTGTCT AAGTTTNAAA TGNNAAGCNN
            901 NNNNNANGAT ATTGNNGCNC NNNNGGNCNN NNNANCATAT GANATTGCNN GNNTNNNNNN
          961 ANNNNNNNNA NNNNNTTNNN NNNNNNN
          //
          Primer: GFP-N_R
          Region: Cycle 3 GFP,attB2,stop codon,insert 3'
          Sequence file: RDB15801_A8Bqf.seq check
          >D05196A8-6_A8Bq_GFP-N_R_F02_2018-03-19-14-24-28_17.ab1
              1 NNNNNNNNNN NNNNNNNNNN NGNNNNNNTC TTCTCCTTTG CTAGCCATTC TAGATCGAAC
             61 CACTTTGTAC AAGAAAGCTG GGTCGGCGCG CCCACCCTTT TAAAACAGAT CATCTTCATC
            121 TGACTCTTCC AGGTACTTTA TAGGTTTCTT TGCCCGTACA GATTTTGCCC GAGGAGCCAC
            181 AGCTGAGTCA AAGTCCATAT GGAAGTCATC ACTCTCCCCC TTGGATTTCT TGCTTGTGAC
            241 TGCTTTCGAA ACAATTTTCT CAAAATTAGA GTCAGAATCA TCAGAAGTGG ATGGCTTCCT
            301 TTTGCGGCGA TTCTTGGTTT TGGCAGGATC AGGCTTTTGA GAGACACCAG AATTCAAAGC
            361 TGGATCCCTT TTAGTTCCTT TTGGGGCAGC CCTTTTTTTG GCACCGGTAG TGGAGGTGGA
            421 AGACTGACTT TTTGCTGCTG TCTTCTTCAC TGTCACATTC TTTTTAGGAA CTGGGTTTGT
            481 AATTTCAGTT TCATCTGGGA AATGTGTAGC AGGAGGGCTT GAAGACAGTG GTACACTGCC
            541 CTTAACATCA TCAGCTTCAA GGTCTGACAC GACACTTTTC TGTGGTTTCA GTTCTTTGTT
            601 ACTAAGTTTT GGGGAAGTTT TGGTCTTAGG TGGACTAGCA TCTGATGGGA CAAAATCTTC
            661 ATCATCAGTT TTTTCATCAA AATCTGAGAA ATCTTCATCT GAATCCAAAT CCATTGTGAA
            721 TTTTGNTTTT GTTGCTGCTC TCCGTGGCTC TGTTTCTCGT GGANGGACAT CAAAATTACT
            781 TTCGTCACTG CTCCTATCTG ANTCTGAATC AGANCAGGGN NTTCTCTTCT TTCCTTTTTT
            841 TGATTGGCTT AAATGCCAAT GNAGTTTGTT TCTTTGNCTT TGTACCTGGT TCTCTTTTCT
            901 GNTTCTTTTC TAATCTTTGN TTTNNGNCTT CNANTTNCNC ACNANNNTNN TTGNNGGNNT
          961 NNNNCANNAT TTTCNNNNNN NANNTTNNNT TNTNNNTTTN NNNTNNNNNN NNNN
          //
          Primer: BGH_rev2
          Region: bGHpA,Cycle 3 GFP
          Sequence file: RDB15801_A8Bqg.seq check
          >D05196A8-6_A8Bq_BGH_rev2_G02_2018-03-19-14-24-28_20.ab1
              1 NNNNNNNNNN NNNNNNNNNG NNNNNNNNNN GGGCNNACNA CAGATGGCTG GCAACTAGAA
             61 GGCACAGTCG AGGCTGATCA GCGGGTTTAA TTCATTATTT GTAGAGCTCA TCCATGCCAT
            121 GTGTAATCCC AGCAGCAGTT ACAAACTCAA GAAGGACCAT GTGGTCACGC TTTTCGTTGG
            181 GATCTTTCGA AAGGGCAGAT TGTGTCGACA GGTAATGGTT GTCTGGTAAA AGGACAGGGC
            241 CATCGCCAAT TGGAGTATTT TGTTGATAAT GGTCTGCTAG TTGAACGGAT CCATCTTCAA
            301 TGTTGTGGCG AATTTTGAAG TTAGCTTTGA TTCCATTCTT TTGTTTGTCT GCCGTGATGT
            361 ATACATTGTG TGAGTTATAG TTGTACTCGA GTTTGTGTCC GAGAATGTTT CCATCTTCTT
            421 TAAAATCAAT ACCTTTTAAC TCGATACGAT TAACAAGGGT ATCACCTTCA AACTTGACTT
            481 CAGCACGCGT CTTGTAGTTC CCGTCATCTT TGAAAGATAT AGTGCGTTCC TGTACATAAC
            541 CTTCGGGCAT GGCACTCTTG AAAAAGTCAT GCCGTTTCAT ATGATCCGGA TAACGGGAAA
            601 AGCATTGAAC ACCATAAGAG AAAGTAGTGA CAAGTGTTGG CCATGGAACA GGTAGTTTTC
            661 CAGTAGTGCA AATAAATTTA AGGGTAAGCT TTCCGTATGT AGCATCACCT TCACCCTCTC
            721 CACTGACAGA AAATTTGTGC CCATTAACAT CACCATCTAA TTCAACAAGA ATTGGGACAA
            781 CTCCAGTGAA AAGTTCTTCT CCTTTGCTAN CCATTCTAGA TCGAACCACT TTGTACAAGA
            841 AAGCTGGGTC GGCGCGCCNA CCCTTTTAAA ACAGATCATC TTCATCTGAN TCTTNNANGT
            901 ACTTNATAGG TTTCTTTGCC CGNACAGATT TTGCCNNAGG ANCCNCANCN GANNNNAANN
          961 NCATATNGNA ANNNATCNCN CTNCCCNNNT NNNNNTNNNT NNNNNNNNNN
          //
          Primer: SV40pro_F
          Region: NeoR
          Sequence file: RDB15801_A8Bqh.seq check
          >D05196A8-6_A8Bq_SV40pro_F_H02_2018-03-19-14-24-28_23.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNTG ANGNNGNTTT TTTGGAGGCC TAGGCTTTTG
             61 CAAAAAGNNN NNGGGNGCTT GTATATCNNT TTTCGGATCT GATCAAGAGA CAGGATGAGG
            121 ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA
            181 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT
            241 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT
            301 GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG
            361 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT
            421 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC
            481 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC
            541 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA
            601 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG
            661 CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT
            721 GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG
            781 CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGNCG GCNAATGGGC
            841 TGANCGCTTC CTCGTGCTTT ACGGNATCGC CGCNCCNNAN TCGCANCGCN TCGCCNNCTA
            901 TCGCCNTCTT GACNANTTCT TCTGANCGGG ANNNNNGGGN NNCNCGAAAT GANNGANCNN
          961 NGCNNNNNCN NNNCNNNNCN NNNNNNANNN TNNNNNNNNN N
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Deguchi, S., Oncogenic effects of evolutionarily conserved noncoding RNA ECONEXIN on gliomagenesis. Oncogene 36 (32): 4629-4640 (2017). PMID 28368417.

          2021.11.25

          GNP_filter3_RDBDEP_html_211111.pl