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pcDNA-DEST47-TOP2A

Gateway(R) expression vector of human TOP2A.

Catalog number RDB15801
Resource name pcDNA-DEST47-TOP2A
Clone info. Gateway(R) expression vector of human topoisomerase 2 alpha (TOP2A) fused with GFP at C-terminal, CMV promoter.
Vector backbone pcDNA-DEST47 (plasmid)
Size of vector backbone 7.8 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human TOP2A cDNA
Depositor|Developer Kondo, Yutaka |
 
Other clones in our bank human TOP2A (NCBI Gene 7153) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Katsushima, K et al. Nat. Commun. 7: 13616, 2016); (Deguchi, S et al. Oncogene. 36 (32) : 4629 - 4640, 2017) RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Schedule A (GFP-A) [Link]
Approval form by depositor [Word]
Remarks ((Additional form)) Approval Form(FormD)
Please use Schedule A (GFP-A) additionally.
Please obtain written approval of the depositor.
Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15801 pcDNA-DEST47-TOP2A DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Deguchi, S., Oncogenic effects of evolutionarily conserved noncoding RNA ECONEXIN on gliomagenesis. Oncogene 36 (32): 4629-4640 (2017). PMID 28368417.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15801_A8Bqp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Region: CMV pro,attB1,insert 5'
Sequence file: RDB15801_A8Bqa.seq check
>D05196A8-6_A8Bq_CMV_Forward_E02_2018-03-19-14-24-28_14.ab1
    1 CGTCAACTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
  121 CTCACTATAG GGAGACCCAA GCTGGCTAGT TAAGCTTGAT CAAACAAGTT TGTACAAAAA
  181 AGCAGGCTCC GCGGCCGCCC CCTTCACCAT GGAAGTGTCA CCATTGCAGC CTGTAAATGA
  241 AAATATGCAA GTCAACAAAA TAAAGAAAAA TGAAGATGCT AAGAAAAGAC TGTCTGTTGA
  301 AAGAATCTAT CAAAAGAAAA CACAATTGGA ACATATTTTG CTCCGCCCAG ACACCTACAT
  361 TGGTTCTGTG GAATTAGTGA CCCAGCAAAT GTGGGTTTAC GATGAAGATG TTGGCATTAA
  421 CTATAGGGAA GTCACTTTTG TTCCTGGTTT GTACAAAATC TTTGATGAGA TTCTAGTTAA
  481 TGCTGCGGAC AACAAACAAA GGGACCCAAA AATGTCTTGT ATTAGAGTCA CAATTGATCC
  541 GGAAAACAAT TTAATTAGTA TATGGAATAA TGGAAAAGGT ATTCCTGTTG TTGAACACAA
  601 AGTTGAAAAG ATGTATGTCC CAGCTCTCAT ATTTGGACAG CTCCTAACTT CTAGTAACTA
  661 TGATGATGAT GAAAAGAAAG TGACAGGTGG TCGAAATGGC TATGGAGCCA AATTGTGTAA
  721 CATATTCAGT ACCAAATTTA CTGTGGAAAC AGCCAGTAGA GAATACAAGA AAATGTTCAA
  781 ACAGACATGG ATGGATAATA TGGGAAGAGC TGGTGAGATG GAACTCAAGC CCTTCAATGG
  841 AGAAGATTAT ACATGTATCA CCTTTCAGCC TGATTTGTCT AAGTTTAAAA TGCAAAGCCT
  901 GGACAAAGAT ATTGTTGCAC TAATGGTCAG AAGAGCATAT GATATTGCTG GGATCCACCA
  961 AAGATGTCAA AGTCTTTTCT TAATGGAAAT AAACTGCCAG TAAAAGGATT TCGTAGTTAT
 1021 GTGGACATGT ATTTGGAAGG ACAAGTTGGG ATGGAACCTG CTTACTGTTG GAAGTATACA
1081 TGAACCAAGT TAAATGACAG GTGGGAAGGG TGGTG
//
Primer: GFP-N_R
Region: Cycle 3 GFP,attB2,insert 3'
Sequence file: RDB15801_A8Bqb.seq check
>D05196A8-6_A8Bq_GFP-N_R_F02_2018-03-19-14-24-28_17.ab1
    1 TATTGGGGGG AAAACTCCAG TGAAAAGTTC TTCTCCTTTG CTAGCCATTC TAGATCGAAC
   61 CACTTTGTAC AAGAAAGCTG GGTCGGCGCG CCCACCCTTT TAAAACAGAT CATCTTCATC
  121 TGACTCTTCC AGGTACTTTA TAGGTTTCTT TGCCCGTACA GATTTTGCCC GAGGAGCCAC
  181 AGCTGAGTCA AAGTCCATAT GGAAGTCATC ACTCTCCCCC TTGGATTTCT TGCTTGTGAC
  241 TGCTTTCGAA ACAATTTTCT CAAAATTAGA GTCAGAATCA TCAGAAGTGG ATGGCTTCCT
  301 TTTGCGGCGA TTCTTGGTTT TGGCAGGATC AGGCTTTTGA GAGACACCAG AATTCAAAGC
  361 TGGATCCCTT TTAGTTCCTT TTGGGGCAGC CCTTTTTTTG GCACCGGTAG TGGAGGTGGA
  421 AGACTGACTT TTTGCTGCTG TCTTCTTCAC TGTCACATTC TTTTTAGGAA CTGGGTTTGT
  481 AATTTCAGTT TCATCTGGGA AATGTGTAGC AGGAGGGCTT GAAGACAGTG GTACACTGCC
  541 CTTAACATCA TCAGCTTCAA GGTCTGACAC GACACTTTTC TGTGGTTTCA GTTCTTTGTT
  601 ACTAAGTTTT GGGGAAGTTT TGGTCTTAGG TGGACTAGCA TCTGATGGGA CAAAATCTTC
  661 ATCATCAGTT TTTTCATCAA AATCTGAGAA ATCTTCATCT GAATCCAAAT CCATTGTGAA
  721 TTTTGTTTTT GTTGCTGCTC TCCGTGGCTC TGTTTCTCGT GGAGGGACAT CAAAATTACT
  781 TTCGTCACTG CTCCTATCTG ATTCTGAATC AGACCAGGGA TTTCTCTTCT TTCCTTTTTT
  841 TGATTGGCTT AAATGCCAAT GTAGTTTGTT TCTTTGTCTT TGTACCTGGT TCTCTTTTCT
  901 GTTTCTTTTC TAATCTTTGT TTTAGGCCTT CTAGTTCCAC ACCATCTTTC TTGAGGGCTT
  961 CCTTCAGTAT TTTCATTCTT AAATTTTCTT TTTTATTTTT TCTTTTCTGC CTCTGCTTTC
 1021 ATTTCTATGA TATTTCGTGG AATGACTCTT TTGACCACGG CGAGGAAGCA AAACTTCAGA
1081 CATTTGTGTT TTTTTTTCCC ATTGGGACCG ATTCCCCCCC TTTGC
//
Primer: BGH_rev2
Region: bGHpA,Cycle 3 GFP,attB2,insert 3'
Sequence file: RDB15801_A8Bqc.seq check
>D05196A8-6_A8Bq_BGH_rev2_G02_2018-03-19-14-24-28_20.ab1
    1 CATCTAGGGC TAGGGGAAGG CACGGGGGAG GGGCAAACAA CAGATGGCTG GCAACTAGAA
   61 GGCACAGTCG AGGCTGATCA GCGGGTTTAA TTCATTATTT GTAGAGCTCA TCCATGCCAT
  121 GTGTAATCCC AGCAGCAGTT ACAAACTCAA GAAGGACCAT GTGGTCACGC TTTTCGTTGG
  181 GATCTTTCGA AAGGGCAGAT TGTGTCGACA GGTAATGGTT GTCTGGTAAA AGGACAGGGC
  241 CATCGCCAAT TGGAGTATTT TGTTGATAAT GGTCTGCTAG TTGAACGGAT CCATCTTCAA
  301 TGTTGTGGCG AATTTTGAAG TTAGCTTTGA TTCCATTCTT TTGTTTGTCT GCCGTGATGT
  361 ATACATTGTG TGAGTTATAG TTGTACTCGA GTTTGTGTCC GAGAATGTTT CCATCTTCTT
  421 TAAAATCAAT ACCTTTTAAC TCGATACGAT TAACAAGGGT ATCACCTTCA AACTTGACTT
  481 CAGCACGCGT CTTGTAGTTC CCGTCATCTT TGAAAGATAT AGTGCGTTCC TGTACATAAC
  541 CTTCGGGCAT GGCACTCTTG AAAAAGTCAT GCCGTTTCAT ATGATCCGGA TAACGGGAAA
  601 AGCATTGAAC ACCATAAGAG AAAGTAGTGA CAAGTGTTGG CCATGGAACA GGTAGTTTTC
  661 CAGTAGTGCA AATAAATTTA AGGGTAAGCT TTCCGTATGT AGCATCACCT TCACCCTCTC
  721 CACTGACAGA AAATTTGTGC CCATTAACAT CACCATCTAA TTCAACAAGA ATTGGGACAA
  781 CTCCAGTGAA AAGTTCTTCT CCTTTGCTAG CCATTCTAGA TCGAACCACT TTGTACAAGA
  841 AAGCTGGGTC GGCGCGCCCA CCCTTTTAAA ACAGATCATC TTCATCTGAC TCTTCCAGGT
  901 ACTTTATAGG TTTCTTTGCC CGTACAGATT TTGCCCGAGG AGCCACAGCT GAGTCAAAGT
  961 CCATATGGGA AGTCATCACT CTCCCCCCTT TGGATTTCTT TGCTTGTGAC TGCTTTCGAA
 1021 CAATTTTCTC AAAATTAGAG GTCAGATCAT CAGAAGTGGG ATGGCTTCGT TTTTGGCGCG
1081 ATTCTTGGTT TGCAGATCAG GCTTTTTGAG AAGAACACCA GAATTCAAAG CTGTGATTC
//
Primer: SV40pro_F
Region: SV40 pro_ori,NeoR
Sequence file: RDB15801_A8Bqd.seq check
>D05196A8-6_A8Bq_SV40pro_F_H02_2018-03-19-14-24-28_23.ab1
    1 ACTCGGTCCC GTAACATTCA GAAGGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG
   61 CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG
  121 ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA
  181 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT
  241 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT
  301 GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG
  361 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT
  421 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC
  481 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC
  541 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA
  601 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG
  661 CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT
  721 GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG
  781 CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC
  841 TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA
  901 TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGG TTCGCGAAAT GACCGACCCA
  961 AGCGACGCCC AACCCTGCCC ATCACGAGAT TTCGATTCCC ACCCGCGCCT TTCTATGAAA
 1021 GGTTGGGCTT TCGGAATTCG TTTTCCGGGA CGCCGGCTGG ATGAATCTTC AGCGCGGGAT
1081 CTCATGCTGA GTCATCGCCA CCCGACTGTT ATTGCCAGCC TTA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Deguchi, S., Oncogenic effects of evolutionarily conserved noncoding RNA ECONEXIN on gliomagenesis. Oncogene 36 (32): 4629-4640 (2017). PMID 28368417.

2020.10.15

GNP_filter3_RDBDEP_html_200316.pl