Please review the QC test results indicated by icon below as well as clone information before placing your order.

pcDNA-DEST47-TOP2A

Gateway(R) expression vector of human TOP2A.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Katsushima, K et al. Nat. Commun. 7: 13616, 2016); (Deguchi, S et al. Oncogene. 36 (32) : 4629 - 4640, 2017). RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form by depositor [Word]
Remarks	((Additional form)) Approval Form(FormD) Please obtain written approval of the depositor. Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.

提供案内 (日本国内) [open/close]

提供条件	利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Katsushima, K et al. Nat. Commun. 7: 13616, 2016); (Deguchi, S et al. Oncogene. 36 (32) : 4629 - 4640, 2017)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 寄託者による提供承諾書 [Word]
備考	【追加提供依頼書】承諾書(FormD) 寄託者の書面による提供承諾が必要です。 Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15801_A8Bqp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro,attB1,insert 5' Sequence file: RDB15801_A8Bqa.seq
>D05196A8-6_A8Bq_CMV_Forward_E02_2018-03-19-14-24-28_14.ab1     1 NNNNNNNNNN NNNCNTNGNN GCNNTGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA    61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA   121 CTCACTATAG GGAGACCCAA GCTGGCTAGT TAAGCTTGAT CAAACAAGTT TGTACAAAAA   181 AGCAGGCTCC GCGGCCGCCC CCTTCACCAT GGAAGTGTCA CCATTGCAGC CTGTAAATGA   241 AAATATGCAA GTCAACAAAA TAAAGAAAAA TGAAGATGCT AAGAAAAGAC TGTCTGTTGA   301 AAGAATCTAT CAAAAGAAAA CACAATTGGA ACATATTTTG CTCCGCCCAG ACACCTACAT   361 TGGTTCTGTG GAATTAGTGA CCCAGCAAAT GTGGGTTTAC GATGAAGATG TTGGCATTAA   421 CTATAGGGAA GTCACTTTTG TTCCTGGTTT GTACAAAATC TTTGATGAGA TTCTAGTTAA   481 TGCTGCGGAC AACAAACAAA GGGACCCAAA AATGTCTTGT ATTAGAGTCA CAATTGATCC   541 GGAAAACAAT TTAATTAGTA TATGGAATAA TGGAAAAGGT ATTCCTGTTG TTGAACACAA   601 AGTTGAAAAG ATGTATGTCC CAGCTCTCAT ATTTGGACAG CTCCTAACTT CTAGTAACTA   661 TGATGATGAT GAAAAGAAAG TGACAGGTGG TCGAAATGGC TATGGAGCCA AATTGTGTAA   721 CATATTCAGT ACCNAATTTA CTGTGGAAAC AGCCAGTAGA GAATACAAGA AAATGTTCNA   781 ACAGACNTGG NTGGATAATA TGGGAAGANC TGGTGAGATG NAACTCNAGC CNNNCNNNGG   841 NNAAGANTAT ACATGTATCA CCTTTCANCC NNNNTTGTCT AAGTTTNAAA TGNNAAGCNN   901 NNNNNANGAT ATTGNNGCNC NNNNGGNCNN NNNANCATAT GANATTGCNN GNNTNNNNNN 961 ANNNNNNNNA NNNNNTTNNN NNNNNNN //
Primer: GFP-N_R (Pr0466) Region: Cycle 3 GFP,attB2,insert 3' Sequence file: RDB15801_A8Bqb.seq
>D05196A8-6_A8Bq_GFP-N_R_F02_2018-03-19-14-24-28_17.ab1     1 NNNNNNNNNN NNNNNNNNNN NGNNNNNNTC TTCTCCTTTG CTAGCCATTC TAGATCGAAC    61 CACTTTGTAC AAGAAAGCTG GGTCGGCGCG CCCACCCTTT TAAAACAGAT CATCTTCATC   121 TGACTCTTCC AGGTACTTTA TAGGTTTCTT TGCCCGTACA GATTTTGCCC GAGGAGCCAC   181 AGCTGAGTCA AAGTCCATAT GGAAGTCATC ACTCTCCCCC TTGGATTTCT TGCTTGTGAC   241 TGCTTTCGAA ACAATTTTCT CAAAATTAGA GTCAGAATCA TCAGAAGTGG ATGGCTTCCT   301 TTTGCGGCGA TTCTTGGTTT TGGCAGGATC AGGCTTTTGA GAGACACCAG AATTCAAAGC   361 TGGATCCCTT TTAGTTCCTT TTGGGGCAGC CCTTTTTTTG GCACCGGTAG TGGAGGTGGA   421 AGACTGACTT TTTGCTGCTG TCTTCTTCAC TGTCACATTC TTTTTAGGAA CTGGGTTTGT   481 AATTTCAGTT TCATCTGGGA AATGTGTAGC AGGAGGGCTT GAAGACAGTG GTACACTGCC   541 CTTAACATCA TCAGCTTCAA GGTCTGACAC GACACTTTTC TGTGGTTTCA GTTCTTTGTT   601 ACTAAGTTTT GGGGAAGTTT TGGTCTTAGG TGGACTAGCA TCTGATGGGA CAAAATCTTC   661 ATCATCAGTT TTTTCATCAA AATCTGAGAA ATCTTCATCT GAATCCAAAT CCATTGTGAA   721 TTTTGNTTTT GTTGCTGCTC TCCGTGGCTC TGTTTCTCGT GGANGGACAT CAAAATTACT   781 TTCGTCACTG CTCCTATCTG ANTCTGAATC AGANCAGGGN NTTCTCTTCT TTCCTTTTTT   841 TGATTGGCTT AAATGCCAAT GNAGTTTGTT TCTTTGNCTT TGTACCTGGT TCTCTTTTCT   901 GNTTCTTTTC TAATCTTTGN TTTNNGNCTT CNANTTNCNC ACNANNNTNN TTGNNGGNNT 961 NNNNCANNAT TTTCNNNNNN NANNTTNNNT TNTNNNTTTN NNNTNNNNNN NNNN //
Primer: BGH_rev2 (Pr0606) Region: bGHpA,Cycle 3 GFP,attB2,insert 3' Sequence file: RDB15801_A8Bqc.seq
>D05196A8-6_A8Bq_BGH_rev2_G02_2018-03-19-14-24-28_20.ab1     1 NNNNNNNNNN NNNNNNNNNG NNNNNNNNNN GGGCNNACNA CAGATGGCTG GCAACTAGAA    61 GGCACAGTCG AGGCTGATCA GCGGGTTTAA TTCATTATTT GTAGAGCTCA TCCATGCCAT   121 GTGTAATCCC AGCAGCAGTT ACAAACTCAA GAAGGACCAT GTGGTCACGC TTTTCGTTGG   181 GATCTTTCGA AAGGGCAGAT TGTGTCGACA GGTAATGGTT GTCTGGTAAA AGGACAGGGC   241 CATCGCCAAT TGGAGTATTT TGTTGATAAT GGTCTGCTAG TTGAACGGAT CCATCTTCAA   301 TGTTGTGGCG AATTTTGAAG TTAGCTTTGA TTCCATTCTT TTGTTTGTCT GCCGTGATGT   361 ATACATTGTG TGAGTTATAG TTGTACTCGA GTTTGTGTCC GAGAATGTTT CCATCTTCTT   421 TAAAATCAAT ACCTTTTAAC TCGATACGAT TAACAAGGGT ATCACCTTCA AACTTGACTT   481 CAGCACGCGT CTTGTAGTTC CCGTCATCTT TGAAAGATAT AGTGCGTTCC TGTACATAAC   541 CTTCGGGCAT GGCACTCTTG AAAAAGTCAT GCCGTTTCAT ATGATCCGGA TAACGGGAAA   601 AGCATTGAAC ACCATAAGAG AAAGTAGTGA CAAGTGTTGG CCATGGAACA GGTAGTTTTC   661 CAGTAGTGCA AATAAATTTA AGGGTAAGCT TTCCGTATGT AGCATCACCT TCACCCTCTC   721 CACTGACAGA AAATTTGTGC CCATTAACAT CACCATCTAA TTCAACAAGA ATTGGGACAA   781 CTCCAGTGAA AAGTTCTTCT CCTTTGCTAN CCATTCTAGA TCGAACCACT TTGTACAAGA   841 AAGCTGGGTC GGCGCGCCNA CCCTTTTAAA ACAGATCATC TTCATCTGAN TCTTNNANGT   901 ACTTNATAGG TTTCTTTGCC CGNACAGATT TTGCCNNAGG ANCCNCANCN GANNNNAANN 961 NCATATNGNA ANNNATCNCN CTNCCCNNNT NNNNNTNNNT NNNNNNNNNN //
Primer: SV40pro_F (Pr0110) Region: SV40 pro_ori,NeoR Sequence file: RDB15801_A8Bqd.seq
>D05196A8-6_A8Bq_SV40pro_F_H02_2018-03-19-14-24-28_23.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNTG ANGNNGNTTT TTTGGAGGCC TAGGCTTTTG    61 CAAAAAGNNN NNGGGNGCTT GTATATCNNT TTTCGGATCT GATCAAGAGA CAGGATGAGG   121 ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA   181 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT   241 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT   301 GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG   361 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT   421 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC   481 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC   541 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA   601 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG   661 CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT   721 GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG   781 CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGNCG GCNAATGGGC   841 TGANCGCTTC CTCGTGCTTT ACGGNATCGC CGCNCCNNAN TCGCANCGCN TCGCCNNCTA   901 TCGCCNTCTT GACNANTTCT TCTGANCGGG ANNNNNGGGN NNCNCGAAAT GANNGANCNN 961 NGCNNNNNCN NNNCNNNNCN NNNNNNANNN TNNNNNNNNN N //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.22

GNP_filter3_RDBDEP_html_230512.pl