RDB15801 - pcDNA-DEST47-TOP2A

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Katsushima, K et al. Nat. Commun. 7: 13616, 2016); (Deguchi, S et al. Oncogene. 36 (32) : 4629 - 4640, 2017) RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Schedule A (GFP-A) [Link] Approval form by depositor [Word]
Remarks	((Additional form)) Approval Form(FormD) Please use Schedule A (GFP-A) additionally. Please obtain written approval of the depositor. Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.

提供案内 (日本国内)

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提供条件	利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Katsushima, K et al. Nat. Commun. 7: 13616, 2016); (Deguchi, S et al. Oncogene. 36 (32) : 4629 - 4640, 2017)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] Schedule A（GFP-A, 英語版のみ） [Link] 寄託者による提供承諾書 [Word]
備考	【追加提供依頼書】承諾書(FormD) Schedule A（GFP-A, 英語版のみ）が追加で必要です。寄託者の書面による提供承諾が必要です。 Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

References and tips

Sequence information

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15801	pcDNA-DEST47-TOP2A	DNA solution

original	Deguchi, S., Oncogenic effects of evolutionarily conserved noncoding RNA ECONEXIN on gliomagenesis. Oncogene 36 (32): 4629-4640 (2017). PMID 28368417.

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check

icon as well as clone information before placing your order.

Primer: CMV_Forward Region: CMV pro,attB1,insert 5' Sequence file: RDB15801_A8Bqe.seq
>D05196A8-6_A8Bq_CMV_Forward_E02_2018-03-19-14-24-28_14.ab1 1 NNNNNNNNNN NNNCNTNGNN GCNNTGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA 61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA 121 CTCACTATAG GGAGACCCAA GCTGGCTAGT TAAGCTTGAT CAAACAAGTT TGTACAAAAA 181 AGCAGGCTCC GCGGCCGCCC CCTTCACCAT GGAAGTGTCA CCATTGCAGC CTGTAAATGA 241 AAATATGCAA GTCAACAAAA TAAAGAAAAA TGAAGATGCT AAGAAAAGAC TGTCTGTTGA 301 AAGAATCTAT CAAAAGAAAA CACAATTGGA ACATATTTTG CTCCGCCCAG ACACCTACAT 361 TGGTTCTGTG GAATTAGTGA CCCAGCAAAT GTGGGTTTAC GATGAAGATG TTGGCATTAA 421 CTATAGGGAA GTCACTTTTG TTCCTGGTTT GTACAAAATC TTTGATGAGA TTCTAGTTAA 481 TGCTGCGGAC AACAAACAAA GGGACCCAAA AATGTCTTGT ATTAGAGTCA CAATTGATCC 541 GGAAAACAAT TTAATTAGTA TATGGAATAA TGGAAAAGGT ATTCCTGTTG TTGAACACAA 601 AGTTGAAAAG ATGTATGTCC CAGCTCTCAT ATTTGGACAG CTCCTAACTT CTAGTAACTA 661 TGATGATGAT GAAAAGAAAG TGACAGGTGG TCGAAATGGC TATGGAGCCA AATTGTGTAA 721 CATATTCAGT ACCNAATTTA CTGTGGAAAC AGCCAGTAGA GAATACAAGA AAATGTTCNA 781 ACAGACNTGG NTGGATAATA TGGGAAGANC TGGTGAGATG NAACTCNAGC CNNNCNNNGG 841 NNAAGANTAT ACATGTATCA CCTTTCANCC NNNNTTGTCT AAGTTTNAAA TGNNAAGCNN 901 NNNNNANGAT ATTGNNGCNC NNNNGGNCNN NNNANCATAT GANATTGCNN GNNTNNNNNN 961 ANNNNNNNNA NNNNNTTNNN NNNNNNN //
Primer: GFP-N_R Region: Cycle 3 GFP,attB2,stop codon,insert 3' Sequence file: RDB15801_A8Bqf.seq
>D05196A8-6_A8Bq_GFP-N_R_F02_2018-03-19-14-24-28_17.ab1 1 NNNNNNNNNN NNNNNNNNNN NGNNNNNNTC TTCTCCTTTG CTAGCCATTC TAGATCGAAC 61 CACTTTGTAC AAGAAAGCTG GGTCGGCGCG CCCACCCTTT TAAAACAGAT CATCTTCATC 121 TGACTCTTCC AGGTACTTTA TAGGTTTCTT TGCCCGTACA GATTTTGCCC GAGGAGCCAC 181 AGCTGAGTCA AAGTCCATAT GGAAGTCATC ACTCTCCCCC TTGGATTTCT TGCTTGTGAC 241 TGCTTTCGAA ACAATTTTCT CAAAATTAGA GTCAGAATCA TCAGAAGTGG ATGGCTTCCT 301 TTTGCGGCGA TTCTTGGTTT TGGCAGGATC AGGCTTTTGA GAGACACCAG AATTCAAAGC 361 TGGATCCCTT TTAGTTCCTT TTGGGGCAGC CCTTTTTTTG GCACCGGTAG TGGAGGTGGA 421 AGACTGACTT TTTGCTGCTG TCTTCTTCAC TGTCACATTC TTTTTAGGAA CTGGGTTTGT 481 AATTTCAGTT TCATCTGGGA AATGTGTAGC AGGAGGGCTT GAAGACAGTG GTACACTGCC 541 CTTAACATCA TCAGCTTCAA GGTCTGACAC GACACTTTTC TGTGGTTTCA GTTCTTTGTT 601 ACTAAGTTTT GGGGAAGTTT TGGTCTTAGG TGGACTAGCA TCTGATGGGA CAAAATCTTC 661 ATCATCAGTT TTTTCATCAA AATCTGAGAA ATCTTCATCT GAATCCAAAT CCATTGTGAA 721 TTTTGNTTTT GTTGCTGCTC TCCGTGGCTC TGTTTCTCGT GGANGGACAT CAAAATTACT 781 TTCGTCACTG CTCCTATCTG ANTCTGAATC AGANCAGGGN NTTCTCTTCT TTCCTTTTTT 841 TGATTGGCTT AAATGCCAAT GNAGTTTGTT TCTTTGNCTT TGTACCTGGT TCTCTTTTCT 901 GNTTCTTTTC TAATCTTTGN TTTNNGNCTT CNANTTNCNC ACNANNNTNN TTGNNGGNNT 961 NNNNCANNAT TTTCNNNNNN NANNTTNNNT TNTNNNTTTN NNNTNNNNNN NNNN //
Primer: BGH_rev2 Region: bGHpA,Cycle 3 GFP Sequence file: RDB15801_A8Bqg.seq
>D05196A8-6_A8Bq_BGH_rev2_G02_2018-03-19-14-24-28_20.ab1 1 NNNNNNNNNN NNNNNNNNNG NNNNNNNNNN GGGCNNACNA CAGATGGCTG GCAACTAGAA 61 GGCACAGTCG AGGCTGATCA GCGGGTTTAA TTCATTATTT GTAGAGCTCA TCCATGCCAT 121 GTGTAATCCC AGCAGCAGTT ACAAACTCAA GAAGGACCAT GTGGTCACGC TTTTCGTTGG 181 GATCTTTCGA AAGGGCAGAT TGTGTCGACA GGTAATGGTT GTCTGGTAAA AGGACAGGGC 241 CATCGCCAAT TGGAGTATTT TGTTGATAAT GGTCTGCTAG TTGAACGGAT CCATCTTCAA 301 TGTTGTGGCG AATTTTGAAG TTAGCTTTGA TTCCATTCTT TTGTTTGTCT GCCGTGATGT 361 ATACATTGTG TGAGTTATAG TTGTACTCGA GTTTGTGTCC GAGAATGTTT CCATCTTCTT 421 TAAAATCAAT ACCTTTTAAC TCGATACGAT TAACAAGGGT ATCACCTTCA AACTTGACTT 481 CAGCACGCGT CTTGTAGTTC CCGTCATCTT TGAAAGATAT AGTGCGTTCC TGTACATAAC 541 CTTCGGGCAT GGCACTCTTG AAAAAGTCAT GCCGTTTCAT ATGATCCGGA TAACGGGAAA 601 AGCATTGAAC ACCATAAGAG AAAGTAGTGA CAAGTGTTGG CCATGGAACA GGTAGTTTTC 661 CAGTAGTGCA AATAAATTTA AGGGTAAGCT TTCCGTATGT AGCATCACCT TCACCCTCTC 721 CACTGACAGA AAATTTGTGC CCATTAACAT CACCATCTAA TTCAACAAGA ATTGGGACAA 781 CTCCAGTGAA AAGTTCTTCT CCTTTGCTAN CCATTCTAGA TCGAACCACT TTGTACAAGA 841 AAGCTGGGTC GGCGCGCCNA CCCTTTTAAA ACAGATCATC TTCATCTGAN TCTTNNANGT 901 ACTTNATAGG TTTCTTTGCC CGNACAGATT TTGCCNNAGG ANCCNCANCN GANNNNAANN 961 NCATATNGNA ANNNATCNCN CTNCCCNNNT NNNNNTNNNT NNNNNNNNNN //
Primer: SV40pro_F Region: NeoR Sequence file: RDB15801_A8Bqh.seq
>D05196A8-6_A8Bq_SV40pro_F_H02_2018-03-19-14-24-28_23.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNTG ANGNNGNTTT TTTGGAGGCC TAGGCTTTTG 61 CAAAAAGNNN NNGGGNGCTT GTATATCNNT TTTCGGATCT GATCAAGAGA CAGGATGAGG 121 ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA 181 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT 241 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT 301 GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG 361 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT 421 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC 481 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC 541 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA 601 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG 661 CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT 721 GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG 781 CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGNCG GCNAATGGGC 841 TGANCGCTTC CTCGTGCTTT ACGGNATCGC CGCNCCNNAN TCGCANCGCN TCGCCNNCTA 901 TCGCCNTCTT GACNANTTCT TCTGANCGGG ANNNNNGGGN NNCNCGAAAT GANNGANCNN 961 NGCNNNNNCN NNNCNNNNCN NNNNNNANNN TNNNNNNNNN N //

pcDNA-DEST47-TOP2A

Distribution information

References and tips

Sequence information

References