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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pmirGLO-TOP2A-3'-UTR

Reporter plasmid of human TOP2A-3'-UTR region for quantitatively evaluate miRNA activity.

Catalog number RDB15800
Resource name pmirGLO-TOP2A-3'-UTR
Clone info. Reporter plasmid of human topoisomerase 2 alpha (TOP2A)-3'-UTR region for quantitatively evaluate miRNA activity.
Comment Expression was confirmed by the depositor with reporter assay.
Vector backbone pmirGLO (plasmid)
Size of vector backbone 7.4 kb
Selectable markers Amp^r
Gene/insert name human TOP2A cDNA
Depositor|Developer Kondo, Yutaka |
Other clones in our bank

External Database
human TOP2A

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Katsushima, K et al. Nat. Commun. 7: 13616, 2016); (Deguchi, S et al. Oncogene. 36 (32) : 4629 - 4640, 2017) RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          Remarks ((Additional form)) Approval Form(FormD)
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          提供条件 利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Katsushima, K et al. Nat. Commun. 7: 13616, 2016); (Deguchi, S et al. Oncogene. 36 (32) : 4629 - 4640, 2017)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。
          付加的提供条件 本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
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          提供依頼書 [Word]
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          寄託者による提供承諾書 [Word]
          備考 【追加提供依頼書】承諾書(FormD)
          寄託者の書面による提供承諾が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15800 pmirGLO-TOP2A-3'-UTR DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Deguchi, S., Oncogenic effects of evolutionarily conserved noncoding RNA ECONEXIN on gliomagenesis. Oncogene 36 (32): 4629-4640 (2017). PMID 28368417.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15800_A8Bqp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: luc2-c_F
          Region: Luc 2 3',insert 5'
          Sequence file: RDB15800_A8Bqa.seq check
          >D05196A7-4_A8Bq_luc2-C_F_D02_2018-03-19-14-24-28_11.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNGNNNNT CTCNTTAAGG CCNAGAAGGG CGGCAAGATC
             61 GCCGTGTAAT TCTAGTTGTT TAAACGAGCT CGCTAGCCTC GAGAATGTGA GGCGATTATT
            121 TTAAGTAATT ATCTTACCAA GCCCAAGACT GGTTTTAAAG TTACCTGAAG CTCTTAACTT
            181 CCTCCCCTCT GAATTTAGTT TGGGGAAGGT GTTTTTAGTA CAAGACATCA AAGTGAAGTA
            241 AAGCCCAAGT GTTCTTTAGC TTTTTATAAT ACTGTCTAAA TAGTGACCAT CTCATGGGCA
            301 TTGTTTTCTT CTCTGCTTTG TCTGTGTTTT GAGTCTGCTT TCTTTTGTCT TTAAAACCTG
            361 ATTTTTAAGT TCTTCTGAAC TGTAGAAATA GCTATCTGAT CACTTCAGCG TAAAGCAGTG
            421 TGTTTATTAA CCATCCACTA AGCTAAAACT AGAGCAGTTT GATTTAAAAG TGTCACTCTT
            481 CCTCCTTTTC TACTTTCAGT AGATATGAGA TAGAGCATAA TTATCTGTTT TATCTTAGTT
            541 TTATACATAA TTTACCATCA GATAGAACTT TATGGTTCTA GTACAGATAC TCTACTACAC
            601 TCAGCCTCTT ATGTGCCAAG TTTTTCTTTA AGCAATGAGA AATTGCTCAT GTTCTTCATC
            661 TTCTCAAATC ATCAGAGGCC GAAGAAAAAC ACTTTGGCTG TGTCTATAAC TTGACACAGT
            721 CAATAGAATG AAGAAAATTA GAGTAGTTAT GTGATTATTT CAGCTCTTGA CCTGTCCCCT
            781 CTGGCTGCCT CTGAGTCTGA ATCTCNCAAA GAGAGAAACC AATTTCTAAG ANGANTGGAT
            841 TGCAGAAGAN TCGGGGNNAA CATTTGATCC NAGATCTTAA ATGNTATATT GATAANNNTG
            901 CTCAGCAATG AGCTATTAGA NTCATTTTGG GAAATCTNNN TNNTTTCNNN TTGTAAACTT
          961 NGNTNANNNN NGNCTANNTT NNNATATGNG NGNNNNTTNN NTNANNNNNN NNNN
          //
          Primer: SV40pA_R
          Region: SV40 pA,insert 3'
          Sequence file: RDB15800_A8Bqb.seq check
          >15800_15800_B0JdF_SV40pA_R_A03_01_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNT TNNNNAATAA AGCATTTTTT TCNCTGCATT CTAGTTGTGG
             61 TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGCTCG AAGCGGCCGC CCCAAGGGGT
            121 TATGCTAGTT ATTGCTCAGC GGTGGCAGCA GCCAACTCAG CTTCCTTTCG GGCTTTGTTA
            181 GCAGCCGGAT CAGCTTGCAT GCCTGCAGGT CGACTCTAGA TTCTATGGGT TGCAATGTTT
            241 AGAACATTTT ATTAAAGTAC AAAATTGTTG GAATTTAGCT AATAGAAAAA CATAGTAAAT
            301 ATTTACAAAA ACGTTGATAA CATTACTCAA GTCACACACA TATAACAATG TAGACAGGTC
            361 TTAACAAAGT TTACAAATTG AAATTATGGA GATTTCCCAA AATGAATCTA ATAGCTCATT
            421 GCTGAGCATG GTTATCAATA TAACATTTAA GATCTTGGAT CAAATGTTGT CCCCGAGTCT
            481 TCTGCAATCC AGTCCTCTTA GAAATTGGTT TCTCTCTTTG GGAGATTCAG ACTCAGAGGC
            541 AGCCAGAGGG GACAGGTCAA GAGCTGAAAT AATCACATAA CTACTCTAAT TTTCTTCATT
            601 CTATTGACTG TGTCAAGTTA TAGACACAGC CNAAGTGNTT TTCTTCGGCC TCTGATGATT
            661 TGAGAAGANG AANNANATGA GCAATTTCTN NTNGCTTNAA GAAAAANNNN NNNNNTNNNA
          721 NNNNNNNNNN
          //
          Primer: SV40pro_F
          Region: hRluc 5'
          Sequence file: RDB15800_A8Bqc.seq check
          >D05196A7-4_A8Bq_SV40pro_F_G01_2018-03-19-14-24-28_19.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGT GNNGNNGNTT TTTTGGAGGC CTAGGCTTTT
             61 GNNNNNAGCT TGATTCTTCT GACNCNNNNN NCTCGAACCA AAGGCTGGAG CCACCATGGC
            121 TTCCAAGGTG TACGACCCCG AGCAACGCAA ACGCATGATC ACTGGGCCTC AGTGGTGGGC
            181 TCGCTGCAAG CAAATGAACG TGCTGGACTC CTTCATCAAC TACTATGATT CCGAGAAGCA
            241 CGCCGAGAAC GCCGTGATTT TTCTGCATGG TAACGCTGCC TCCAGCTACC TGTGGAGGCA
            301 CGTCGTGCCT CACATCGAGC CCGTGGCTAG ATGCATCATC CCTGATCTGA TCGGAATGGG
            361 TAAGTCCGGC AAGAGCGGGA ATGGCTCATA TCGCCTCCTG GATCACTACA AGTACCTCAC
            421 CGCTTGGTTC GAGCTGCTGA ACCTTCCAAA GAAAATCATC TTTGTGGGCC ACGACTGGGG
            481 GGCTTGTCTG GCCTTTCACT ACTCCTACGA GCACCAAGAC AAGATCAAGG CCATCGTCCA
            541 TGCTGAGAGT GTCGTGGACG TGATCGAGTC CTGGGACGAG TGGCCTGACA TCGAGGAGGA
            601 TATCGCCCTG ATCAAGAGCG AAGAGGGCGA GAAAATGGTG CTTGAGAATA ACTTCTTCGT
            661 CGAGACCATG CTCCCAAGCA AGATCATGCG GAAACTGGAG CCTGAGGAGT TCGCTGCCTA
            721 CCTGGAGCNN TTCAAGGAGA AGGGCGAGGT TAGACGGCCT ANCCTCTCCT GNCCTCGCGA
            781 NATCCCTCTC GTTAAGGGAN GCAAGCCCGA CGTCGTCCAG ATTGTCCGCA ACTACNACGN
            841 CTACCTTCGG GCCANCGNCG ATCTGCCTAA GATGTTCATC GANTCCGANC CNGGGTTCTT
            901 TTNCNNCNCT ATTGTCNAGG GNNCTNAGAN NTTCCCTAAC NNNNNNTTCG TGNNNNNGNN
          961 NGNNNTNNNN TTCAGCNNNN NNNNNN
          //
          Primer: Neo-M_R
          Region: NeoR 5',hRluc 3'
          Sequence file: RDB15800_A8Bqd.seq check
          >D05196A7-4_A8Bq_Neo-M_R_A02_2018-03-19-14-24-28_02.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNTNNNNNT TNNGTGACNA CGTCGAGCAC AGCTGCGCAA
             61 GGAACGCCCG TCGTGGCCNG CCACGATAGC CGCGCTGCCT CGTCCTGCAG TTCATTCAGG
            121 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC
            181 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC
            241 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCCCTCC GGAGCCTCCG
            301 CCACCTGATC CGCCACCTCC GCTCCCACCA CCGGTCTGCT CGTTCTTCAG CACGCGCTCC
            361 ACGAAGCTCT TGATGTACTT ACCCATTTCA TCTGGAGCGT CCTCCTGGCT GAAGTGGAGG
            421 CCCTTCACCT TCACGAACTC GGTGTTAGGG AACTTCTTAG CTCCCTCGAC AATAGCGTTG
            481 GAAAAGAACC CAGGGTCGGA CTCGATGAAC ATCTTAGGCA GATCGTCGCT GGCCCGAAGG
            541 TAGGCGTTGT AGTTGCGGAC AATCTGGACG ACGTCGGGCT TGCCTCCCTT AACGAGAGGG
            601 ATCTCGCGAG GCCAGGAGAG GGTAGGCCGT CTAACCTCGC CCTTCTCCTT GAATGGCTCC
            661 AGGTAGGCAG CGAACTCCTC ANGCTCCAGT TTCCGCATGA TCTTGCTTGG GAGCATGGTC
            721 TCGACGAAGA AGTTATTCTC AAGCACCATT TTCTCGCCCT CTTCGCTCTT GATCAGGGCG
            781 ATATCCTCCT CNATGTCANG NCACTCGTCC CAGGANTCNA TCACGTNCAC GANACTCTCA
            841 GCATGGACNA TGGNCTTGAT CTTGNCTTGG NGCNCGTNNN ANTAGTGAAA NGNNCAGANN
            901 AGCCCCCCNN GNNNNNNNNC NNNNGNTGAT TTNCNTNNGN NNNNNNANCA GNNNNNNCCN
          961 AGCNNNNANG NNNNNNNNNN
          //
          Primer: PGKpro_F
          Region: Luc2 5'
          Sequence file: RDB15800_A8Bqe.seq check
          >D05196A7-4_A8Bq_PGKprp_F_C02_2018-03-19-14-24-28_08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNTCTCCNGG CCTTTCGNNC TGCAGCCCAA GCTTGGCAAT
             61 CCGGTACTGT TGGTANAGCC ACCATGGAAG ATGCCAAAAA CATTAAGAAG GGCCCAGCGC
            121 CATTCTACCC ACTCGAAGAC GGGACCGCCG GCGAGCAGCT GCACAAAGCC ATGAAGCGCT
            181 ACGCCCTGGT GCCCGGCACC ATCGCCTTTA CCGACGCACA TATCGAGGTG GACATTACCT
            241 ACGCCGAGTA CTTCGAGATG AGCGTTCGGC TGGCAGAAGC TATGAAGCGC TATGGGCTGA
            301 ATACAAACCA TCGGATCGTG GTGTGCAGCG AGAATAGCTT GCAGTTCTTC ATGCCCGTGT
            361 TGGGTGCCCT GTTCATCGGT GTGGCTGTGG CCCCAGCTAA CGACATCTAC AACGAGCGCG
            421 AGCTGCTGAA CAGCATGGGC ATCAGCCAGC CCACCGTCGT ATTCGTGAGC AAGAAAGGGC
            481 TGCAAAAGAT CCTCAACGTG CAAAAGAAGC TACCGATCAT ACAAAAGATC ATCATCATGG
            541 ATAGCAAGAC CGACTACCAG GGCTTCCAAA GCATGTACAC CTTCGTGACT TCCCATTTGC
            601 CACCCGGCTT CAACGAGTAC GACTTCGTGC CCGAGAGCTT CGACCGGGAC AAAACCATCG
            661 CCCTGATCAT GAACAGTAGT GGCAGTACCG GATTGCCCAA GGGCGTAGCC CTACCGCACC
            721 GCACCGCTTG TGTCCGATTC AGTCATGCCC GCGACCCCAT CTTCGGCAAC CAGATCATCC
            781 CCGACACCGC TATCCTCAGC GTGGTGCCAT TTCACNACGG CTTCGGCATG TTCACCACGC
            841 NNGGCTACTT GATCTGCGGC TTTCGGGNCG TGCTCATGTA CCGCTTCNAG GANGANCTAT
            901 TCTTGCGCAG CTNGNNNGAN TATAANATTN NNTCTGNCNN NCNNGNNNCC ACNCTATTTN
          961 NCNNNNTCNC NNANNNCNNN NNNATCNNNN NNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Deguchi, S., Oncogenic effects of evolutionarily conserved noncoding RNA ECONEXIN on gliomagenesis. Oncogene 36 (32): 4629-4640 (2017). PMID 28368417.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl