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p3xFLAG-Myc-CMV-AGO2-PAZ-mut

Expression vector of human AGO2 mutated PAZ domain (Y311Q/F312L).

Catalog number RDB15798
Resource name p3xFLAG-Myc-CMV-AGO2-PAZ-mut
Clone info. Expression vector of human Argonaute protein 2 (AGO2) mutated PAZ domain (Y311Q/F312L), tagged with FLAG at N-terminus, CMV promoter. Please note Myc-tag is not available. See Fig. 4b of Deguchi, S. et al., Oncogene, 36 (32): 4629-4640, 2017 for outline of construction.
Expression was confirmed by the depositor with western blotting.
Vector backbone p3xFLAG-Myc-CMV (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human AGO2 cDNA
Depositor Kondo, Yutaka |
 
Other clones in our bank human AGO2 (NCBI Gene 27161) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Katsushima, K et al. Nat. Commun. 7: 13616, 2016); (Deguchi, S et al. Oncogene. 36 (32) : 4629 - 4640, 2017) RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB15798 p3xFLAG-Myc-CMV-AGO2-PAZ-mut Under QC test. Please contact us. DNA solution

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References and tips

Electronic file

Original reference

original Deguchi, S., Oncogenic effects of evolutionarily conserved noncoding RNA ECONEXIN on gliomagenesis. Oncogene 36 (32): 4629-4640 (2017). PMID 28368417.

Further references such as user reports and related articles (go to bottom)

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Deguchi, S., Oncogenic effects of evolutionarily conserved noncoding RNA ECONEXIN on gliomagenesis. Oncogene 36 (32): 4629-4640 (2017). PMID 28368417.

2019.07.17

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