Expression vector of human AGO2 wild type.
Clone info. | Expression vector of human Argonaute protein 2 (AGO2), wild type, tagged with FLAG at N-terminus, CMV promoter. Please note Myc-tag is not available. See Fig. 4b of Deguchi, S. et al., Oncogene, 36 (32): 4629-4640, 2017 for outline of construction. |
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Comment | Expression was confirmed by the depositor with western blotting. |
Vector backbone | p3xFLAG-Myc-CMV (plasmid) |
Selectable markers | Amp^r (E. coli), Neo^r (mammalian cells). |
Gene/insert name | human AGO2 cDNA |
Depositor|Developer | Kondo, Yutaka | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() MTA, for use for for-profit purpose [Word ![]() Approval form by depositor [Word ![]() Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Katsushima, K et al. Nat. Commun. 7: 13616, 2016); (Deguchi, S et al. Oncogene. 36 (32) : 4629 - 4640, 2017). RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. |
Remarks | ((Additional form)) Approval Form(FormD) Please obtain written approval of the depositor. |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 提供同意書 (MTA, 営利目的用)[Word ![]() 寄託者による提供承諾書 [Word ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Katsushima, K et al. Nat. Commun. 7: 13616, 2016); (Deguchi, S et al. Oncogene. 36 (32) : 4629 - 4640, 2017)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。 |
備考 | 【追加提供依頼書】承諾書(FormD) 寄託者の書面による提供承諾が必要です。 |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB15797 | p3xFLAG-Myc-CMV-AGO2-WT | Under QC test. Please contact us. | DNA solution |
Materials & Methods section:
The p3xFLAG-Myc-CMV-AGO2-WT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15797). |
Reference section:
Deguchi, S., Katsushima, K., Hatanaka, A., Shinjo, K., Ohka, F., Wakabayashi, T., Zong, H., Natsume, A., Kondo, Y., Oncogenic effects of evolutionarily conserved noncoding RNA ECONEXIN on gliomagenesis. Oncogene 36 (32): 4629-4640 (2017). PMID 28368417. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
original | Deguchi, S., Oncogenic effects of evolutionarily conserved noncoding RNA ECONEXIN on gliomagenesis. Oncogene 36 (32): 4629-4640 (2017). PMID 28368417. [link to RRC of NBRP] |
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