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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAGGS-BLRP-BirA (#RDB15774)

Expression vector to perform ChIP-seq analysis using biotinylated proteins.

Clone info. Insertion of an ORF to this plasmids vector results in expression of a biotinylated proteins in animal cells, which is suitable for high-sensitivity ChIP-seq analyses. SmaI site is available to make target protein plus C-terminal fusion of biotin ligase recognition peptide (BLRP). Please refer Fig.1A and Fig.S5 of Matsuda, K. et al., Development 144 (11): 1948-1958 (2017) for the constitution and nucleotide sequence of pCAGGS-BLRP-BirA, respectively.
Comment Expression was confirmed by the depositor with Western blot.
Vector backbone pCAGGS (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Gene/insert name E. coli biotin ligase genomic DNA
Depositor|Developer Kondoh, Hisato | Boss, Jeremy M. |
 
Sequence (full) RDB15774zzk02.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Matsuda, K. et al., Development 144 (11): 1948-1958 (2017)).
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Matsuda, K. et al., Development 144 (11): 1948-1958 (2017))。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15774 pCAGGS-BLRP-BirA DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCAGGS-BLRP-BirA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15774).

Reference section:

Matsuda, K., Mikami, T., Oki, S., Iida, H., Andrabi, M., Boss, J.M., Yamaguchi, K., Shigenobu, S., Kondoh, H., ChIP-seq analysis of genomic binding regions of five major transcription factors highlights a central role for ZIC2 in the mouse epiblast stem cell gene regulatory network. Development 144 (11): 1948-1958 (2017). PMID 28455373. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15774_A8Avp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F_V2 (Pr0330)
Region: CAG pro,BLRP,6XHis,IRES
Sequence file: RDB15774_A8Ava.seq check
>15774_15774_A8Av_pCAG-F_V2_H02_23_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGNG CGGNNNCNAN TCTGGGNNGC GCCGCCGCAC CCCNTCTAGC
   61 GGGCGCGGGG CGAAGCGGTG CGGCGCCGGC AGGAAGGAAA TGGGCGGGGA GGGCCTTCGT
  121 GCGTCGCCGC GCCGCCGTCC CCTTCTCCCT CTCCAGCCTC GGGGCTGTCC GCGGGGGGAC
  181 GGCTGCCTTC GGGGGGGACG GGGCAGGGCG GGGTTCGGCT TCTGGCGTGT GACCGGCGGC
  241 TCTAGAGCCT CTGCTAACCA TGTTCATGCC TTCTTCTTTT TCCTACAGCT CCTGGGCAAC
  301 GTGCTGGTTA TTGTGCTGTC TCATCATTTT GGCAAAGAAT TGCACCCCCG GGATGGCTGG
  361 TGGCCTCAAC GACATCTTCG AGGCCCAGAA GATCGAGTGG CATGAAGATA CTGGTGGATC
  421 AGAGAATTTG TATTTTCAGT CTCATCATCA CCATCACCAT GCTAGCGGCC GCTAAGCAGA
  481 TCTGCGGCCG CTCGAGGAAT TCTCTCCCTC CCCCCCCCCT AACGTTACTG GCCGAAGCCG
  541 CTTGGAATAA GGCCGGTGTG CGTTTGTCTA TATGTTATTT TCCACCATAT TGCCGTCTTT
  601 TGGCAATGTG AGGGCCCGGA AACCTGGCCC TGTCTTCTTG ACGAGCATTC CTAGGGGTCT
  661 TTCCCCTCTC GCCAAAGGAA TGCAAGGTCT GTTGAATGTC GTGAAGGAAG CAGTTCCTCT
  721 GGAAGCTTCT TGAAGANAAA CAACGTCTGT AGCGANNNTT TGCAGGCAGC GGANNNNNNN
  781 NACCTGNCGA CAGGTGNNTC TGCGGNCAAA AGCCACGTGT ATAANATACA CCTGNAAANG
  841 CGGCACAACC NCNGNGCCNC GTTGTGAGTT GGATANTTGT GNAAAGANNN AAATGGNNNN
901 NNNNNN
//
Primer: IRES-F (Pr0036)
Region: IRES,BirA 5'
Sequence file: RDB15774_A8Avb.seq check
>15774_15774_A8Av_IRES-F_A03_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NCGANGTNNN NNNACGTCTA GGCCCCCCNA ACCACGGGGN
   61 NGTGGTTTTC CTTTGAAAAA CACGATGATA ATATGGCCAC ACTCGAGGCC GGCAAGGCCG
  121 GATCCACCAT GAAGGATAAC ACCGTGCCAC TGAAATTGAT TGCCCTGTTA GCGAACGGTG
  181 AATTTCACTC TGGCGAGCAG TTGGGTGAAA CGCTGGGAAT GAGCCGGGCG GCTATTAATA
  241 AACACATTCA GACACTGCGT GACTGGGGCG TTGATGTCTT TACCGTTCCG GGTAAAGGAT
  301 ACAGCCTGCC TGAGCCTATC CAGTTACTTA ATGCTAAACA GATATTGGGT CAGCTGGATG
  361 GCGGTAGTGT AGCCGTGCTG CCAGTGATTG ACTCCACGAA TCAGTACCTT CTTGATCGTA
  421 TCGGAGAGCT TAAATCGGGC GATGCTTGCA TTGCAGAATA CCAGCAGGCT GGCCGTGGTC
  481 GCCGGGGTCG GAAATGGTTT TCGCCTTTTG GCGCAAACTT ATATTTGTCG ATGTTCTGGC
  541 GTCTGGAACA AGGCCCGGCG GCGGCGATTG GTTTAAGTCT GGTTATCGGT ATCGTGATGG
  601 CGGAAGTATT ACGCAAGCTG GGTGCAGATA AAGTTCGTGT TAAATGGCCT AATGACCTCT
  661 ATCTGCAGGA TCGCAAGCTG GCAGGCATTC TGGTGGAGCT GACTGGCAAA ACTGGCGATG
  721 CGGCGCNNNT AGTCATTGGA GCCGGGATCA ACATGGCAAT GCGCCGTGTT GAAGAGAGTG
  781 TCGTTAATCA NNNGTGGATC ACGCTGCANG ANNCGGGGNT CAATCTCGAT CGTANTACGT
  841 TGGCGGCCAT GCTAATACGT GAATTACGNG CTGCNTNGGA ACTCTTCNAN CAANAANNNN
  901 NNNNNCNNTA NCTGNNNCGC NNGNNAANGC NGGANANTTT TATTNNNCNC CNNNNNNANN
961 NNNNNNNN
//
Primer: pCA-bgter (Pr0066)
Region: BirA 3'
Sequence file: RDB15774_A8Avc.seq check
>15774_15774_A8Av_pCA-bgter_B03_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN TTTGGNNNNN GNNNNNNNNC TCAGNGGTAT TTGTGAGCCA
   61 GGGCATTGGC CACACCAGCC ACCACCTTCT GATAGGCAGC CTGCACCTGA GGAGTGAATT
  121 TTATTTTTCT GCACTACGCA GGGATATTTC ACCGCCCATC CAGGGTTTTA TTATTCCATC
  181 CTGCTCAAGT AATAAAGCCC CCTGTTTGTC TATTCCGCGT GAAATGCCAA ATATTTCTTT
  241 ATCACCAATG ATAAGTTTCA CTGGGCGATT AATAAAATTA TCCAGCTTTT CCCAGCGCGA
  301 CAGATAAGGT GCCAATCCTT CTTGTTCGAA GAGTTCCAAC GCAGCACGTA ATTCACGTAT
  361 TAGCATGGCC GCCAACGTAT TACGATCGAG ATTGATCCCC GCTTCCTGCA GCGTGATCCA
  421 CCCCTGATTA ACGACACTCT CTTCAACACG GCGCATTGCC ATGTTGATCC CGGCTCCAAT
  481 GACTATTTGC GCCGCATCGC CAGTTTTGCC AGTCAGCTCC ACCAGAATGC CTGCCAGCTT
  541 GCGATCCTGC AGATAGAGGT CATTAGGCCA TTTAACACGA ACTTTATCTG CACCCAGCTT
  601 GCGTAATACT TCCGCCATCA CGATACCGAT AACCAGACTT AAACCAATCG CCGCCGCCGG
  661 GCCTTGTTCC AGACGCCAGA ACATCGACAA ATATAAGTTT GCGCCAAAAG GCGAAAACCA
  721 TTTCCGANCC CGGCGACCAC GGCCAGCCTG CTGGTATTCT GCAATGCAAG CATCGCCCGA
  781 NTTAAGCTCT CCGATACGAT CAAGAANGNA CTGATTCGTG NANTCNATCA CNGGNAGCAC
  841 GGCTACNNNA CCGCCNTCCN GCNGANCCNA TATCTGTTNA NNANNNANTA ANNGNATANG
  901 NNCNNGNNNN CNGTATNNTT TANNNNGNNN NGNAAANNNA TCNNNNCCCC NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Matsuda, K., ChIP-seq analysis of genomic binding regions of five major transcription factors highlights a central role for ZIC2 in the mouse epiblast stem cell gene regulatory network. Development 144 (11): 1948-1958 (2017). PMID 28455373. [link to RRC of NBRP]
user_report Komai, M., Nuclear SphK2/S1P signaling is a key regulator of ApoE production and Aβ uptake in astrocytes. J. Lipid Res. 65 (3): 100510 (2024). PMID 38280459. [link to RRC of NBRP]