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pCAGGS-BLRP-BirA

Expression vector to perform ChIP-seq analysis using biotinylated proteins.

Catalog number RDB15774
Resource name pCAGGS-BLRP-BirA
Clone info. Insertion of an ORF to this plasmids vector results in expression of a biotinylated proteins in animal cells, which is suitable for high-sensitivity ChIP-seq analyses. SmaI site is available to make target protein plus C-terminal fusion of biotin ligase recognition peptide (BLRP). Please refer Fig.1A and Fig.S5 of Matsuda, K. et al., Development 144 (11): 1948-1958 (2017) for the constitution and nucleotide sequence of pCAGGS-BLRP-BirA, respectively.
Expression was confirmed by the depositor with Western blot.
Vector backbone pCAGGS (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Gene/insert name E. coli biotin ligase genomic DNA
Depositor Kondoh, Hisato | Boss, Jeremy M. |
 
Other clones in our bank E. coli BirA (KEGG orthology K03524) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Matsuda, K. et al., Development 144 (11): 1948-1958 (2017)).
Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
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RDB15774 pCAGGS-BLRP-BirA DNA solution

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References and tips

Electronic file

Featured content

References

original Matsuda, K., ChIP-seq analysis of genomic binding regions of five major transcription factors highlights a central role for ZIC2 in the mouse epiblast stem cell gene regulatory network. Development 144 (11): 1948-1958 (2017). PMID 28455373.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15774_A8Avp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: oCAG-F_V2 RDB15774_A8Ava.seq
>15774_15774_A8Av_pCAG-F_V2_H02_23_ABI24.ab1
    1 GCATGTTGGT CAAATCTGTG CGGAGCCGAA TCTGGGAGGC GCCGCCGCAC CCCCTCTAGC
   61 GGGCGCGGGG CGAAGCGGTG CGGCGCCGGC AGGAAGGAAA TGGGCGGGGA GGGCCTTCGT
  121 GCGTCGCCGC GCCGCCGTCC CCTTCTCCCT CTCCAGCCTC GGGGCTGTCC GCGGGGGGAC
  181 GGCTGCCTTC GGGGGGGACG GGGCAGGGCG GGGTTCGGCT TCTGGCGTGT GACCGGCGGC
  241 TCTAGAGCCT CTGCTAACCA TGTTCATGCC TTCTTCTTTT TCCTACAGCT CCTGGGCAAC
  301 GTGCTGGTTA TTGTGCTGTC TCATCATTTT GGCAAAGAAT TGCACCCCCG GGATGGCTGG
  361 TGGCCTCAAC GACATCTTCG AGGCCCAGAA GATCGAGTGG CATGAAGATA CTGGTGGATC
  421 AGAGAATTTG TATTTTCAGT CTCATCATCA CCATCACCAT GCTAGCGGCC GCTAAGCAGA
  481 TCTGCGGCCG CTCGAGGAAT TCTCTCCCTC CCCCCCCCCT AACGTTACTG GCCGAAGCCG
  541 CTTGGAATAA GGCCGGTGTG CGTTTGTCTA TATGTTATTT TCCACCATAT TGCCGTCTTT
  601 TGGCAATGTG AGGGCCCGGA AACCTGGCCC TGTCTTCTTG ACGAGCATTC CTAGGGGTCT
  661 TTCCCCTCTC GCCAAAGGAA TGCAAGGTCT GTTGAATGTC GTGAAGGAAG CAGTTCCTCT
  721 GGAAGCTTCT TGAAGACAAA CAACGTCTGT AGCGACCCTT TGCAGGCAGC GGAACCCCCC
  781 CACCTGGCGA CAGGTGCCTC TGCGGCCAAA AGCCACGTGT ATAAGATACA CCTGCAAAGG
  841 CGGCACAACC CCAGTGCCAC GTTGTGAGTT GGATAGTTGT GGAAAGAGTC AAATGGCTCT
  901 CCTCAAGCGT ATTCAACAAG GGGCTGAAAG GATGCCCAGA AAGGTACCCC CATTGTATGG
  961 ATCTGATCTG GGGGCCTCGG GTGCACATGC TTTACATGTG TTTAGTCGAG GTTAAAAAAA
 1021 AACGTCTAGC TCTCCTCGAA CAACGGGGAC GTAATTTTTC GATTGAAAAA CACGAATGAA
1081 TAATATGGCC ACACCTTCGA AG
//
Primer: IRES-F RDB15774_A8Avb.seq
>15774_15774_A8Av_IRES-F_A03_03_ABI24.ab1
    1 GCGTTCTTTT CTGTGTTTAG TCGAGGTTAA AAAACGTCTA GGCCCCCCGA ACCACGGGGA
   61 CGTGGTTTTC CTTTGAAAAA CACGATGATA ATATGGCCAC ACTCGAGGCC GGCAAGGCCG
  121 GATCCACCAT GAAGGATAAC ACCGTGCCAC TGAAATTGAT TGCCCTGTTA GCGAACGGTG
  181 AATTTCACTC TGGCGAGCAG TTGGGTGAAA CGCTGGGAAT GAGCCGGGCG GCTATTAATA
  241 AACACATTCA GACACTGCGT GACTGGGGCG TTGATGTCTT TACCGTTCCG GGTAAAGGAT
  301 ACAGCCTGCC TGAGCCTATC CAGTTACTTA ATGCTAAACA GATATTGGGT CAGCTGGATG
  361 GCGGTAGTGT AGCCGTGCTG CCAGTGATTG ACTCCACGAA TCAGTACCTT CTTGATCGTA
  421 TCGGAGAGCT TAAATCGGGC GATGCTTGCA TTGCAGAATA CCAGCAGGCT GGCCGTGGTC
  481 GCCGGGGTCG GAAATGGTTT TCGCCTTTTG GCGCAAACTT ATATTTGTCG ATGTTCTGGC
  541 GTCTGGAACA AGGCCCGGCG GCGGCGATTG GTTTAAGTCT GGTTATCGGT ATCGTGATGG
  601 CGGAAGTATT ACGCAAGCTG GGTGCAGATA AAGTTCGTGT TAAATGGCCT AATGACCTCT
  661 ATCTGCAGGA TCGCAAGCTG GCAGGCATTC TGGTGGAGCT GACTGGCAAA ACTGGCGATG
  721 CGGCGCAAAT AGTCATTGGA GCCGGGATCA ACATGGCAAT GCGCCGTGTT GAAGAGAGTG
  781 TCGTTAATCA GGGGTGGATC ACGCTGCAGG AAGCGGGGAT CAATCTCGAT CGTAATACGT
  841 TGGCGGCCAT GCTAATACGT GAATTACGTG CTGCGTTGGA ACTCTTCGAA CAAGAAGGAT
  901 TGGCACCTTA TCTGTCGCGC TGGGAAAAGC TGGATAATTT TATTAATCGC CCAGTGAAAC
  961 TTATCATTGG TGATAAAGAA ATATTTTGGC ATTTCACGCG GCATAGACAA CAGGGGGGCT
 1021 TTTATTACTT TGAGCAGGAT GGAATATAAA CCCTGCATGG GCCGATTGAA ATATCCTGCC
1081 GTTAGTGCCA GAAAAATTTA AAAATC
//
Primer: pCA-bgter RDB15774_A8Avc.seq
>15774_15774_A8Av_pCA-bgter_B03_06_ABI24.ab1
    1 AGCCCAGAAG TCCAATAATT TTTGGCAGAG GGAAAAGATC TCAGTGGTAT TTGTGAGCCA
   61 GGGCATTGGC CACACCAGCC ACCACCTTCT GATAGGCAGC CTGCACCTGA GGAGTGAATT
  121 TTATTTTTCT GCACTACGCA GGGATATTTC ACCGCCCATC CAGGGTTTTA TTATTCCATC
  181 CTGCTCAAGT AATAAAGCCC CCTGTTTGTC TATTCCGCGT GAAATGCCAA ATATTTCTTT
  241 ATCACCAATG ATAAGTTTCA CTGGGCGATT AATAAAATTA TCCAGCTTTT CCCAGCGCGA
  301 CAGATAAGGT GCCAATCCTT CTTGTTCGAA GAGTTCCAAC GCAGCACGTA ATTCACGTAT
  361 TAGCATGGCC GCCAACGTAT TACGATCGAG ATTGATCCCC GCTTCCTGCA GCGTGATCCA
  421 CCCCTGATTA ACGACACTCT CTTCAACACG GCGCATTGCC ATGTTGATCC CGGCTCCAAT
  481 GACTATTTGC GCCGCATCGC CAGTTTTGCC AGTCAGCTCC ACCAGAATGC CTGCCAGCTT
  541 GCGATCCTGC AGATAGAGGT CATTAGGCCA TTTAACACGA ACTTTATCTG CACCCAGCTT
  601 GCGTAATACT TCCGCCATCA CGATACCGAT AACCAGACTT AAACCAATCG CCGCCGCCGG
  661 GCCTTGTTCC AGACGCCAGA ACATCGACAA ATATAAGTTT GCGCCAAAAG GCGAAAACCA
  721 TTTCCGACCC CGGCGACCAC GGCCAGCCTG CTGGTATTCT GCAATGCAAG CATCGCCCGA
  781 TTTAAGCTCT CCGATACGAT CAAGAAGGTA CTGATTCGTG GAGTCAATCA CTGGCAGCAC
  841 GGCTACACTA CCGCCATCCA GCTGACCCAA TATCTGTTTA GCATTAAGTA ACTGGATAGG
  901 CTCAGGCAGG CTGTATCCTT TACCCGGAAC GGTAAAGACA TCAACGCCCC CAGTCACGCA
  961 GTGTCTGAAT GTGTTTATTT AATAGCCGCC CGGCTCATTC CCAGCGTTTC ACCCCAAACT
 1021 GCTCGCAGAG GTGAAATTCA CCGTTCGCTT ACAGCAATTC ACTTTCAGTG CACGATGTAA
1081 TCGTTCATGG GTGATCCGGG CCC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Matsuda, K., ChIP-seq analysis of genomic binding regions of five major transcription factors highlights a central role for ZIC2 in the mouse epiblast stem cell gene regulatory network. Development 144 (11): 1948-1958 (2017). PMID 28455373.

2018.08.31

GNP_filter3_RDBDEP_html_180823.pl