Sequencing and PCR primers for primer information.
Expression vector to perform ChIP-seq analysis using biotinylated proteins.
| Catalog number | RDB15774 |
|---|---|
| Resource name | pCAGGS-BLRP-BirA |
| Clone info. | Insertion of an ORF to this plasmids vector results in expression of a biotinylated proteins in animal cells, which is suitable for high-sensitivity ChIP-seq analyses. SmaI site is available to make target protein plus C-terminal fusion of biotin ligase recognition peptide (BLRP). Please refer Fig.1A and Fig.S5 of Matsuda, K. et al., Development 144 (11): 1948-1958 (2017) for the constitution and nucleotide sequence of pCAGGS-BLRP-BirA, respectively. Expression was confirmed by the depositor with Western blot. |
| Vector backbone | pCAGGS (Plasmid) |
| Size of vector backbone | 4.8 kb |
| Selectable markers | Amp^r |
| Gene/insert name | E. coli biotin ligase genomic DNA |
| Depositor | Kondoh, Hisato | Boss, Jeremy M. | |
| Other clones in our bank | E. coli BirA (KEGG orthology K03524) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Matsuda, K. et al., Development 144 (11): 1948-1958 (2017)). |
|---|---|
| Additional terms and conditions for distribution | Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested. |
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB15774 | pCAGGS-BLRP-BirA | DNA solution |
Electronic file
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References
User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB15774_A8Avp1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: oCAG-F_V2 | RDB15774_A8Ava.seq |
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>15774_15774_A8Av_pCAG-F_V2_H02_23_ABI24.ab1 1 GCATGTTGGT CAAATCTGTG CGGAGCCGAA TCTGGGAGGC GCCGCCGCAC CCCCTCTAGC 61 GGGCGCGGGG CGAAGCGGTG CGGCGCCGGC AGGAAGGAAA TGGGCGGGGA GGGCCTTCGT 121 GCGTCGCCGC GCCGCCGTCC CCTTCTCCCT CTCCAGCCTC GGGGCTGTCC GCGGGGGGAC 181 GGCTGCCTTC GGGGGGGACG GGGCAGGGCG GGGTTCGGCT TCTGGCGTGT GACCGGCGGC 241 TCTAGAGCCT CTGCTAACCA TGTTCATGCC TTCTTCTTTT TCCTACAGCT CCTGGGCAAC 301 GTGCTGGTTA TTGTGCTGTC TCATCATTTT GGCAAAGAAT TGCACCCCCG GGATGGCTGG 361 TGGCCTCAAC GACATCTTCG AGGCCCAGAA GATCGAGTGG CATGAAGATA CTGGTGGATC 421 AGAGAATTTG TATTTTCAGT CTCATCATCA CCATCACCAT GCTAGCGGCC GCTAAGCAGA 481 TCTGCGGCCG CTCGAGGAAT TCTCTCCCTC CCCCCCCCCT AACGTTACTG GCCGAAGCCG 541 CTTGGAATAA GGCCGGTGTG CGTTTGTCTA TATGTTATTT TCCACCATAT TGCCGTCTTT 601 TGGCAATGTG AGGGCCCGGA AACCTGGCCC TGTCTTCTTG ACGAGCATTC CTAGGGGTCT 661 TTCCCCTCTC GCCAAAGGAA TGCAAGGTCT GTTGAATGTC GTGAAGGAAG CAGTTCCTCT 721 GGAAGCTTCT TGAAGACAAA CAACGTCTGT AGCGACCCTT TGCAGGCAGC GGAACCCCCC 781 CACCTGGCGA CAGGTGCCTC TGCGGCCAAA AGCCACGTGT ATAAGATACA CCTGCAAAGG 841 CGGCACAACC CCAGTGCCAC GTTGTGAGTT GGATAGTTGT GGAAAGAGTC AAATGGCTCT 901 CCTCAAGCGT ATTCAACAAG GGGCTGAAAG GATGCCCAGA AAGGTACCCC CATTGTATGG 961 ATCTGATCTG GGGGCCTCGG GTGCACATGC TTTACATGTG TTTAGTCGAG GTTAAAAAAA 1021 AACGTCTAGC TCTCCTCGAA CAACGGGGAC GTAATTTTTC GATTGAAAAA CACGAATGAA 1081 TAATATGGCC ACACCTTCGA AG // |
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| Primer: IRES-F | RDB15774_A8Avb.seq |
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>15774_15774_A8Av_IRES-F_A03_03_ABI24.ab1 1 GCGTTCTTTT CTGTGTTTAG TCGAGGTTAA AAAACGTCTA GGCCCCCCGA ACCACGGGGA 61 CGTGGTTTTC CTTTGAAAAA CACGATGATA ATATGGCCAC ACTCGAGGCC GGCAAGGCCG 121 GATCCACCAT GAAGGATAAC ACCGTGCCAC TGAAATTGAT TGCCCTGTTA GCGAACGGTG 181 AATTTCACTC TGGCGAGCAG TTGGGTGAAA CGCTGGGAAT GAGCCGGGCG GCTATTAATA 241 AACACATTCA GACACTGCGT GACTGGGGCG TTGATGTCTT TACCGTTCCG GGTAAAGGAT 301 ACAGCCTGCC TGAGCCTATC CAGTTACTTA ATGCTAAACA GATATTGGGT CAGCTGGATG 361 GCGGTAGTGT AGCCGTGCTG CCAGTGATTG ACTCCACGAA TCAGTACCTT CTTGATCGTA 421 TCGGAGAGCT TAAATCGGGC GATGCTTGCA TTGCAGAATA CCAGCAGGCT GGCCGTGGTC 481 GCCGGGGTCG GAAATGGTTT TCGCCTTTTG GCGCAAACTT ATATTTGTCG ATGTTCTGGC 541 GTCTGGAACA AGGCCCGGCG GCGGCGATTG GTTTAAGTCT GGTTATCGGT ATCGTGATGG 601 CGGAAGTATT ACGCAAGCTG GGTGCAGATA AAGTTCGTGT TAAATGGCCT AATGACCTCT 661 ATCTGCAGGA TCGCAAGCTG GCAGGCATTC TGGTGGAGCT GACTGGCAAA ACTGGCGATG 721 CGGCGCAAAT AGTCATTGGA GCCGGGATCA ACATGGCAAT GCGCCGTGTT GAAGAGAGTG 781 TCGTTAATCA GGGGTGGATC ACGCTGCAGG AAGCGGGGAT CAATCTCGAT CGTAATACGT 841 TGGCGGCCAT GCTAATACGT GAATTACGTG CTGCGTTGGA ACTCTTCGAA CAAGAAGGAT 901 TGGCACCTTA TCTGTCGCGC TGGGAAAAGC TGGATAATTT TATTAATCGC CCAGTGAAAC 961 TTATCATTGG TGATAAAGAA ATATTTTGGC ATTTCACGCG GCATAGACAA CAGGGGGGCT 1021 TTTATTACTT TGAGCAGGAT GGAATATAAA CCCTGCATGG GCCGATTGAA ATATCCTGCC 1081 GTTAGTGCCA GAAAAATTTA AAAATC // |
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| Primer: pCA-bgter | RDB15774_A8Avc.seq |
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>15774_15774_A8Av_pCA-bgter_B03_06_ABI24.ab1 1 AGCCCAGAAG TCCAATAATT TTTGGCAGAG GGAAAAGATC TCAGTGGTAT TTGTGAGCCA 61 GGGCATTGGC CACACCAGCC ACCACCTTCT GATAGGCAGC CTGCACCTGA GGAGTGAATT 121 TTATTTTTCT GCACTACGCA GGGATATTTC ACCGCCCATC CAGGGTTTTA TTATTCCATC 181 CTGCTCAAGT AATAAAGCCC CCTGTTTGTC TATTCCGCGT GAAATGCCAA ATATTTCTTT 241 ATCACCAATG ATAAGTTTCA CTGGGCGATT AATAAAATTA TCCAGCTTTT CCCAGCGCGA 301 CAGATAAGGT GCCAATCCTT CTTGTTCGAA GAGTTCCAAC GCAGCACGTA ATTCACGTAT 361 TAGCATGGCC GCCAACGTAT TACGATCGAG ATTGATCCCC GCTTCCTGCA GCGTGATCCA 421 CCCCTGATTA ACGACACTCT CTTCAACACG GCGCATTGCC ATGTTGATCC CGGCTCCAAT 481 GACTATTTGC GCCGCATCGC CAGTTTTGCC AGTCAGCTCC ACCAGAATGC CTGCCAGCTT 541 GCGATCCTGC AGATAGAGGT CATTAGGCCA TTTAACACGA ACTTTATCTG CACCCAGCTT 601 GCGTAATACT TCCGCCATCA CGATACCGAT AACCAGACTT AAACCAATCG CCGCCGCCGG 661 GCCTTGTTCC AGACGCCAGA ACATCGACAA ATATAAGTTT GCGCCAAAAG GCGAAAACCA 721 TTTCCGACCC CGGCGACCAC GGCCAGCCTG CTGGTATTCT GCAATGCAAG CATCGCCCGA 781 TTTAAGCTCT CCGATACGAT CAAGAAGGTA CTGATTCGTG GAGTCAATCA CTGGCAGCAC 841 GGCTACACTA CCGCCATCCA GCTGACCCAA TATCTGTTTA GCATTAAGTA ACTGGATAGG 901 CTCAGGCAGG CTGTATCCTT TACCCGGAAC GGTAAAGACA TCAACGCCCC CAGTCACGCA 961 GTGTCTGAAT GTGTTTATTT AATAGCCGCC CGGCTCATTC CCAGCGTTTC ACCCCAAACT 1021 GCTCGCAGAG GTGAAATTCA CCGTTCGCTT ACAGCAATTC ACTTTCAGTG CACGATGTAA 1081 TCGTTCATGG GTGATCCGGG CCC // |
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Please visit Sequencing and PCR primers for primer information.
References
2018.11.21
GNP_filter3_RDBDEP_html_181012.pl