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pHSP70-EGFP-TRAIL

Heat-shock-inducible fusion expression vector of EGFP-TRAIL.

Catalog number RDB15766
Resource name pHSP70-EGFP-TRAIL
Clone info. Heat-shock-inducible fusion expression vector of EGFP-Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL). Promoter region corresponds -456 to -6-bp region (relative to translation start) of human HSP70b' (heat shock protein family A member 6 (HSPA6), NCBI Accession No. DQ521571.1).
Expression was confirmed by the depositor with fluorescence and phototoxicity assay.
Vector backbone pEGFP-N1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Human HSPA6 genomic DNA Human TNFSF10 cDNA
Depositor Kengaku, Mineko |
 
Other clones in our bank human HSPA6 (NCBI Gene 3310) | human TNFSF10 (NCBI Gene 8743) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nakatsuji, H. et al. Sci. Rep. 7(1): 4694, 2017)
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB15766 pHSP70-EGFP-TRAIL Under QC test. Please contact us. DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Nakatsuji, H., Surface chemistry for cytosolic gene delivery and photothermal transgene expression by gold nanorods. Sci. Rep. 7 (1): 4694 (2017). PMID 28680130.

Further references such as user reports and related articles (go to bottom)

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Nakatsuji, H., Surface chemistry for cytosolic gene delivery and photothermal transgene expression by gold nanorods. Sci. Rep. 7 (1): 4694 (2017). PMID 28680130.

2019.07.17

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