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pHSP70-EGFP-TRAIL

Heat-shock-inducible fusion expression vector of EGFP-TRAIL.

Catalog number RDB15766
Resource name pHSP70-EGFP-TRAIL
Clone info. Heat-shock-inducible fusion expression vector of EGFP-Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL). Promoter region corresponds -456 to -6-bp region (relative to translation start) of human HSP70b' (heat shock protein family A member 6 (HSPA6), NCBI Accession No. DQ521571.1).
Comment Expression was confirmed by the depositor with fluorescence and phototoxicity assay.
Vector backbone pEGFP-N1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Human HSPA6 genomic DNA Human TNFSF10 cDNA
Depositor|Developer Kengaku, Mineko |
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External Database
Human HSPA6 Human TNFSF10

        Reference sequence
          

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nakatsuji, H. et al. Sci. Rep. 7(1): 4694, 2017)
        Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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        Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
        This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
        提供案内 (日本国内) [open/close]

        提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Nakatsuji, H. et al. Sci. Rep. 7(1): 4694, 2017)。
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
        提供依頼書 [Word]
        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
        GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

        Catalog # Resource name Availability Shipping form Fee (non-profit org.)
        RDB15766 pHSP70-EGFP-TRAIL Under QC test. Please contact us. DNA solution

        check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

        References and tips

        Electronic file

        Original reference

        original Nakatsuji, H., Surface chemistry for cytosolic gene delivery and photothermal transgene expression by gold nanorods. Sci. Rep. 7 (1): 4694 (2017). PMID 28680130.

        Further references such as user reports and related articles (go to bottom)

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        Sequence information

        check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


        References

        Original, user report and related articles

        original Nakatsuji, H., Surface chemistry for cytosolic gene delivery and photothermal transgene expression by gold nanorods. Sci. Rep. 7 (1): 4694 (2017). PMID 28680130.

        2022.07.13

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