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pHSP70-EGFP-TRAIL (#RDB15766)

Heat-shock-inducible fusion expression vector of EGFP-TRAIL.

Clone info. Heat-shock-inducible fusion expression vector of EGFP-Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL). Promoter region corresponds -456 to -6-bp region (relative to translation start) of human HSP70b' (heat shock protein family A member 6 (HSPA6), NCBI Accession No. DQ521571.1).
Comment Expression was confirmed by the depositor with fluorescence and phototoxicity assay.
Vector backbone pEGFP-N1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Human HSPA6 genomic DNA Human TNFSF10 cDNA
Depositor|Developer Kengaku, Mineko |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nakatsuji, H. et al. Sci. Rep. 7(1): 4694, 2017)
提供案内 (日本国内) [open/close]

必要書類
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提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Nakatsuji, H. et al. Sci. Rep. 7(1): 4694, 2017)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB15766 pHSP70-EGFP-TRAIL Under QC test. Please contact us. DNA solution

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How to cite this biological resource

Materials & Methods section:

The pHSP70-EGFP-TRAIL was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15766).

Reference section:

Nakatsuji, H., Kawabata Galbraith, K., Kurisu, J., Imahori, H., Murakami, T., Kengaku, M., Surface chemistry for cytosolic gene delivery and photothermal transgene expression by gold nanorods. Sci. Rep. 7 (1): 4694 (2017). PMID 28680130. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Nakatsuji, H., Surface chemistry for cytosolic gene delivery and photothermal transgene expression by gold nanorods. Sci. Rep. 7 (1): 4694 (2017). PMID 28680130. [link to RRC of NBRP]