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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pHSP70-EGFP

Heat-shock-inducible EGFP reporter construct.

Catalog number RDB15765
Resource name pHSP70-EGFP
Clone info. Heat-shock-inducible reporter construct. EGFP was placed under the control of human HSP70b' (heat shock protein family A member 6 (HSPA6)) promoter corresponding -456 to -6-bp region (relative to translation start) of human HSPA6 (NCBI Accession No. DQ521571.1). See Fig. 3 of Nakatsuji, H. et al., Sci. Rep., 7 (1): 4694, 2017.
Comment Expression was confirmed by the depositor with fluorescence assay. Known differences: A to C substitution at 2222nt of DQ521571.1.
Vector backbone pEGFP-N1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Human HSPA6 genomic DNA
Depositor|Developer Kengaku, Mineko |
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External Database
Human HSPA6

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nakatsuji, H. et al. Sci. Rep. 7(1): 4694, 2017)
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Nakatsuji, H. et al. Sci. Rep. 7(1): 4694, 2017)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15765 pHSP70-EGFP DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Nakatsuji, H., Surface chemistry for cytosolic gene delivery and photothermal transgene expression by gold nanorods. Sci. Rep. 7 (1): 4694 (2017). PMID 28680130.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15765_A8Ajp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EGFP-N
          Sequence file: RDB15765_A8Aja.seq check
          >D05107A1_A8Aj_1_EGFP-N_A08_01_ABI08.ab1
              1 GTACCCGGGG CTTACGCTCC TCGCCCTTGC TCACCATGGT GGCGACCGGT GGATCCCGGG
             61 CCCGCGGTAC CGTCGACTGC AGAATTCGAA GCTTCTTGTC GGATGCTGGA GGCCACGGAG
            121 GCTGCTGCCG CTCCGCCGTC GAGGGGCGCT CAGCAGTGAG GCTCTCCCTG CGGTTTCTCT
            181 GCAGCCAGCC CGGCTCGGAT CTGCTCAGCG CCGCTTCCAC GGGCTTTTTA TCCTTTTGCG
            241 CCTCACCCGA CCCCCGCCGC CGCGAACTTT CGCGAACCTT TCCGCACCTT CCCGCACCTT
            301 CCCGCCCAGT TGAGAGCCTC TCGCCCGCCC GGGCTGACTC AGCCGGGTGG CGCGGCGCGG
            361 CGCTGACCCC GGGAGAAGGT TCGGGCAGAT CGGGGCATGA GCCGCGAGGC TGGGCGGCTA
            421 CGCCAGGCCA GCCCGCCTCT GACTCAGGCT GCTGAAAGAA ATGCGGGGAA GGTTCTAGCT
            481 CCTCCGGGCT GGCTCTGAGG ACGCGCGGTT GTGGCAGAGG CTGCAAAAGC AGCAACTTTA
            541 GAGGATTAAT AACTAATGCA TGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC
            601 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT
            661 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG
            721 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC
            781 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC
            841 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT
            901 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA AACCCCCCCG TTCAGCCCGA CCGCTGCGCC
            961 TTATCCGGGT AACTATCGTC TTTGAGTCCC AACCCCGGGT AAGGACACGA CTTTATCGCC
           1021 ACTGGGCAGC AAGCCAACTT GGTAACAGGA TTTAGCAGAG CGAAGGTTAT GTAAGCGGTG
           1081 CTAACAGAGT TCTTTGAAGG TGTGGCTTAA CTTACGGCTA CACTAGGAGA GCCAGGTATA
          1141 TTTTGGGGT
          //
          Primer: pAxEF R2
          Sequence file: RDB15765_A8Ajb.seq check
          >D05107A1_A8Aj_1_pAxEF_R2_B08_02_ABI08.ab1
              1 CGTGGGTAAA ATGCTTTATT TGTGAATTTG TGATGCTATT GCTTTATTTG TAACCATTAT
             61 AAGCTGCAAT AAACAAGTTA ACAACAACAA TTGCATTCAT TTTATGTTTC AGGTTCAGGG
            121 GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA AAACCTCTAC AAATGTGGTA TGGCTGATTA
            181 TGATCTAGAG TCGCGGCCGC TTTACTTGTA CAGCTCGTCC ATGCCGAGAG TGATCCCGGC
            241 GGCGGTCACG AACTCCAGCA GGACCATGTG ATCGCGCTTC TCGTTGGGGT CTTTGCTCAG
            301 GGCGGACTGG GTGCTCAGGT AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT CGCCGATGGG
            361 GGTGTTCTGC TGGTAGTGGT CGGCGAGCTG CACGCTGCCG TCCTCGATGT TGTGGCGGAT
            421 CTTGAAGTTC ACCTTGATGC CGTTCTTCTG CTTGTCGGCC ATGATATAGA CGTTGTGGCT
            481 GTTGTAGTTG TACTCCAGCT TGTGCCCCAG GATGTTGCCG TCCTCCTTGA AGTCGATGCC
            541 CTTCAGCTCG ATGCGGTTCA CCAGGGTGTC GCCCTCGAAC TTCACCTCGG CGCGGGTCTT
            601 GTAGTTGCCG TCGTCCTTGA AGAAGATGGT GCGCTCCTGG ACGTAGCCTT CGGGCATGGC
            661 GGACTTGAAG AAGTCGTGCT GCTTCATGTG GTCGGGGTAG CGGCTGAAGC ACTGCACGCC
            721 GTAGGTCAGG GTGGTCACGA GGGTGGGCCA GGGCACGGGC AGCTTGCCGG TGGTGCAGAT
            781 GAACTTCAGG GTCAGCTTGC CGTAGGTGGC ATCGCCCTCG CCCTCGCCGG ACACGCTGAA
            841 CTTGTGGCCG TTTACGTCGC CGTCCAGCTC GACCAGGATG GGCACCACCC CGGTGAACAG
            901 CTCCTCGCCC TTGCTCACCA TGGTGGCGAC CGGTGGATCC CGGGCCCGCG GTACCGTCGA
            961 CTGCAGAATT CGAAGCTTCT TGTCGGATGC TGGAGGCCAC GGAAGGCTGC TGCCGCTCCG
           1021 GCCGTCGAGG GGCGGCTTCA GCAGTGAGGC TCCTCCCTGC GGGATTCTTC TGCAGCCAGC
           1081 CCTGGCCTCG GAATCTGCTT CAGCGGTCGC ATCCACGGAC TTTTTTATCC TTTTTTGGCG
          1141 TCCTTCATCC TCGA
          //
          Primer: SV40pro_F
          Sequence file: RDB15765_A8Ajc.seq check
          >D05107A1_A8Aj_1_SV40pro_F_C08_03_ABI08.ab1
              1 GGTTCGGGGG TTAGCCTTTC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG
             61 CAAAGATCGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC
            121 ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA
            181 CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT
            241 TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA AGACGAGGCA GCGCGGCTAT
            301 CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG
            361 GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG
            421 CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC
            481 CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA
            541 TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG
            601 CCGAACTGTT CGCCAGGCTC AAGGCGAGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC
            661 ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG
            721 ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA
            781 TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG
            841 CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC
            901 TCTGGGGTTC GAAATGACCG ACCCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT
            961 CCACCGCCGC CTTCTATGAA AGGGTTGGGG CTTCTGAATC GTTTTCCGGG ACGCCCGGCT
           1021 GGGATGATCC CTCCAGCGCG GGGGATCTCA TGCTGGGAGG TTCTTTCGCC CCACCCTACG
           1081 GGGAGCTAAC TGAACACGAG AGACCATTAC TGGAAGGAAC CCGCCGCCTA GTGACGGGCC
          1141 TAAT
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Nakatsuji, H., Surface chemistry for cytosolic gene delivery and photothermal transgene expression by gold nanorods. Sci. Rep. 7 (1): 4694 (2017). PMID 28680130.

          2022.07.13

          GNP_filter3_RDBDEP_html_220713.pl