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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pHSP70-EGFP (#RDB15765)

Heat-shock-inducible EGFP reporter construct.

Clone info. Heat-shock-inducible reporter construct. EGFP was placed under the control of human HSP70b' (heat shock protein family A member 6 (HSPA6)) promoter corresponding -456 to -6-bp region (relative to translation start) of human HSPA6 (NCBI Accession No. DQ521571.1). See Fig. 3 of Nakatsuji, H. et al., Sci. Rep., 7 (1): 4694, 2017.
Comment Expression was confirmed by the depositor with fluorescence assay. Known differences: A to C substitution at 2222nt of DQ521571.1.
Vector backbone pEGFP-N1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Human HSPA6 genomic DNA
Depositor|Developer Kengaku, Mineko |
 

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nakatsuji, H. et al. Sci. Rep. 7(1): 4694, 2017)
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RDB15765 pHSP70-EGFP DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pHSP70-EGFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15765).

Reference section:

Nakatsuji, H., Kawabata Galbraith, K., Kurisu, J., Imahori, H., Murakami, T., Kengaku, M., Surface chemistry for cytosolic gene delivery and photothermal transgene expression by gold nanorods. Sci. Rep. 7 (1): 4694 (2017). PMID 28680130. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15765_A8Ajp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-N ()
Sequence file: RDB15765_A8Aja.seq check
>D05107A1_A8Aj_1_EGFP-N_A08_01_ABI08.ab1
    1 GTACCCGGGG CTTACGCTCC TCGCCCTTGC TCACCATGGT GGCGACCGGT GGATCCCGGG
   61 CCCGCGGTAC CGTCGACTGC AGAATTCGAA GCTTCTTGTC GGATGCTGGA GGCCACGGAG
  121 GCTGCTGCCG CTCCGCCGTC GAGGGGCGCT CAGCAGTGAG GCTCTCCCTG CGGTTTCTCT
  181 GCAGCCAGCC CGGCTCGGAT CTGCTCAGCG CCGCTTCCAC GGGCTTTTTA TCCTTTTGCG
  241 CCTCACCCGA CCCCCGCCGC CGCGAACTTT CGCGAACCTT TCCGCACCTT CCCGCACCTT
  301 CCCGCCCAGT TGAGAGCCTC TCGCCCGCCC GGGCTGACTC AGCCGGGTGG CGCGGCGCGG
  361 CGCTGACCCC GGGAGAAGGT TCGGGCAGAT CGGGGCATGA GCCGCGAGGC TGGGCGGCTA
  421 CGCCAGGCCA GCCCGCCTCT GACTCAGGCT GCTGAAAGAA ATGCGGGGAA GGTTCTAGCT
  481 CCTCCGGGCT GGCTCTGAGG ACGCGCGGTT GTGGCAGAGG CTGCAAAAGC AGCAACTTTA
  541 GAGGATTAAT AACTAATGCA TGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC
  601 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT
  661 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG
  721 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC
  781 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC
  841 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT
  901 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA AACCCCCCCG TTCAGCCCGA CCGCTGCGCC
  961 TTATCCGGGT AACTATCGTC TTTGAGTCCC AACCCCGGGT AAGGACACGA CTTTATCGCC
 1021 ACTGGGCAGC AAGCCAACTT GGTAACAGGA TTTAGCAGAG CGAAGGTTAT GTAAGCGGTG
 1081 CTAACAGAGT TCTTTGAAGG TGTGGCTTAA CTTACGGCTA CACTAGGAGA GCCAGGTATA
1141 TTTTGGGGT
//
Primer: pAxEF R2 ()
Sequence file: RDB15765_A8Ajb.seq check
>D05107A1_A8Aj_1_pAxEF_R2_B08_02_ABI08.ab1
    1 CGTGGGTAAA ATGCTTTATT TGTGAATTTG TGATGCTATT GCTTTATTTG TAACCATTAT
   61 AAGCTGCAAT AAACAAGTTA ACAACAACAA TTGCATTCAT TTTATGTTTC AGGTTCAGGG
  121 GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA AAACCTCTAC AAATGTGGTA TGGCTGATTA
  181 TGATCTAGAG TCGCGGCCGC TTTACTTGTA CAGCTCGTCC ATGCCGAGAG TGATCCCGGC
  241 GGCGGTCACG AACTCCAGCA GGACCATGTG ATCGCGCTTC TCGTTGGGGT CTTTGCTCAG
  301 GGCGGACTGG GTGCTCAGGT AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT CGCCGATGGG
  361 GGTGTTCTGC TGGTAGTGGT CGGCGAGCTG CACGCTGCCG TCCTCGATGT TGTGGCGGAT
  421 CTTGAAGTTC ACCTTGATGC CGTTCTTCTG CTTGTCGGCC ATGATATAGA CGTTGTGGCT
  481 GTTGTAGTTG TACTCCAGCT TGTGCCCCAG GATGTTGCCG TCCTCCTTGA AGTCGATGCC
  541 CTTCAGCTCG ATGCGGTTCA CCAGGGTGTC GCCCTCGAAC TTCACCTCGG CGCGGGTCTT
  601 GTAGTTGCCG TCGTCCTTGA AGAAGATGGT GCGCTCCTGG ACGTAGCCTT CGGGCATGGC
  661 GGACTTGAAG AAGTCGTGCT GCTTCATGTG GTCGGGGTAG CGGCTGAAGC ACTGCACGCC
  721 GTAGGTCAGG GTGGTCACGA GGGTGGGCCA GGGCACGGGC AGCTTGCCGG TGGTGCAGAT
  781 GAACTTCAGG GTCAGCTTGC CGTAGGTGGC ATCGCCCTCG CCCTCGCCGG ACACGCTGAA
  841 CTTGTGGCCG TTTACGTCGC CGTCCAGCTC GACCAGGATG GGCACCACCC CGGTGAACAG
  901 CTCCTCGCCC TTGCTCACCA TGGTGGCGAC CGGTGGATCC CGGGCCCGCG GTACCGTCGA
  961 CTGCAGAATT CGAAGCTTCT TGTCGGATGC TGGAGGCCAC GGAAGGCTGC TGCCGCTCCG
 1021 GCCGTCGAGG GGCGGCTTCA GCAGTGAGGC TCCTCCCTGC GGGATTCTTC TGCAGCCAGC
 1081 CCTGGCCTCG GAATCTGCTT CAGCGGTCGC ATCCACGGAC TTTTTTATCC TTTTTTGGCG
1141 TCCTTCATCC TCGA
//
Primer: SV40pro_F ()
Sequence file: RDB15765_A8Ajc.seq check
>D05107A1_A8Aj_1_SV40pro_F_C08_03_ABI08.ab1
    1 GGTTCGGGGG TTAGCCTTTC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG
   61 CAAAGATCGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC
  121 ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA
  181 CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT
  241 TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA AGACGAGGCA GCGCGGCTAT
  301 CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG
  361 GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG
  421 CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC
  481 CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA
  541 TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG
  601 CCGAACTGTT CGCCAGGCTC AAGGCGAGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC
  661 ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG
  721 ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA
  781 TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG
  841 CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC
  901 TCTGGGGTTC GAAATGACCG ACCCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT
  961 CCACCGCCGC CTTCTATGAA AGGGTTGGGG CTTCTGAATC GTTTTCCGGG ACGCCCGGCT
 1021 GGGATGATCC CTCCAGCGCG GGGGATCTCA TGCTGGGAGG TTCTTTCGCC CCACCCTACG
 1081 GGGAGCTAAC TGAACACGAG AGACCATTAC TGGAAGGAAC CCGCCGCCTA GTGACGGGCC
1141 TAAT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Nakatsuji, H., Surface chemistry for cytosolic gene delivery and photothermal transgene expression by gold nanorods. Sci. Rep. 7 (1): 4694 (2017). PMID 28680130. [link to RRC of NBRP]