Sequencing and PCR primers for primer information.
Fluorescent nuclear marker, broader distribution in nucleus and leaky in cytoplasm.
| Catalog number | RDB15741 |
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| Resource name | GFP-NLS PARP |
| Clone info. | Fluorescent nuclear marker, broader distribution in nucleus and leaky in cytoplasm: expression vector of GFP, tagged with a single NLS sequence of poly (ADP-ribose) polymerase (PARP) at C-terminal. Expression was confirmed by the depositor with microscopic observation. Utilized EGFP with monomeric mutation (A206K, D.A. Zacharias et al., Science. 296 (5569): 913-916, 2002). |
| Vector backbone | pEGFP-C1 (plasmid) |
| Gene/insert name | cDNA |
| Depositor | Kinjo, Masataka | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kitamura, A. et al., Biochem. Biophys. Res. Commun., 463 (3): 401-406, 2015). |
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| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link] |
| Remarks | Please use Schedule A (GFP-A) additionally. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB15741 | GFP-NLS PARP | DNA solution |
Electronic file
Original reference
| original | Kitamura, A., Efficient and dynamic nuclear localization of green fluorescent protein via RNA binding. Biochem. Biophys. Res. Commun. 463 (3): 401-406 (2015). PMID 26032495. |
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Further references such as user reports and related articles (go to bottom)
Featured content
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB15741_A8Anp1-2.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Region: Sv40p(A),PARP NLS,mEGFP | Primer: SV40polyA_R2 | Sequence: RDB15741_A8Ana.seq |
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>D05001A3_A8An_4_SV40polyA-R2_C02_03_ABI08.ab1 1 GCAAATTAAT AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA 61 GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT 121 GGCTGATTAT GATCAGTTAT CTAGATCCGG TGGATCCCGG GCCCGCGGTA CCGTCGACTG 181 CAGAATTCGA AGCTTCGGCT TTCAGCGCTT TTTCCAGTTT GCTATCTTTA TCCTTTTCTT 241 TCTTGCTCTT CTTTTTGGCC ACTTCGCCGC CTTTGCGTTT GCCTTCGCTT TTCACAGATC 301 TGAGTCCGGA CTTGTACAGC TCGTCCATGC CGAGAGTGAT CCCGGCGGCG GTCACGAACT 361 CCAGCAGGAC CATGTGATCG CGCTTCTCGT TGGGGTCTTT GCTCAGCTTG GACTGGGTGC 421 TCAGGTAGTG GTTGTCGGGC AGCAGCACGG GGCCGTCGCC GATGGGGGTG TTCTGCTGGT 481 AGTGGTCGGC GAGCTGCACG CTGCCGTCCT CGATGTTGTG GCGGATCTTG AAGTTCACCT 541 TGATGCCGTT CTTCTGCTTG TCGGCCATGA TATAGACGTT GTGGCTGTTG TAGTTGTACT 601 CCAGCTTGTG CCCCAGGATG TTGCCGTCCT CCTTGAAGTC GATGCCCTTC AGCTCGATGC 661 GGTTCACCAG GGTGTCGCCC TCGAACTTCA CCTCGGCGCG GGTCTTGTAG TTGCCGTCGT 721 CCTTGAAGAA GATGGTGCGC TCCTGGACGT AGCCTTCGGG CATGGCGGAC TTGAAGAAGT 781 CGTGCTGCTT CATGTGGTCG GGGTAGCGGC TGAAGCACTG CACGCCGTAG GTCAGGGTGG 841 TCACGAGGGT GGGCCAGGGC ACGGGCAGCT TGCCGGTGGT GCAGATGAAC TTCAGGGTCA 901 GCTTGCGTAG TGGCATCGCC CTCGCCCTCG CCGGACAACG CTGAACTTGT GGCCGTTTAC 961 CGTCGCCGTC CAACTCGACC AGGATGGGCA ACACCCCGGT GACAGCTCTC GCCCTTGGCT 1021 CACCATGGTG GCGACCGGGT ACCGCTTACC GGATCTGTAC GGTTCACTAA ACCCAGCTCC 1081 TGCTTATATA TGACCTCCCA CCCGTTCACG CCTAACCGCC CCAATTTGCT TTCAATGGGG 1141 GCGGAAGTTG TTTCGAAACA TTTTGGAAGT CCCCGTTTGA TTTTGGGTGG CCAAAATCAA 1201 CCTTCCAATT GAACGTCTTA TGGGGGTTGG TAGAGCTTTG GGGAATACTC CGCCTCG // |
| Region: CMV pro,mEGFP,PARP NLS | Primer: CMV_Forward | Sequence: RDB15741_A8Anb.seq |
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>D05001A3-4_A8An_CMV-Forward_G02_20_ABI24.ab1 1 CATCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG 61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGTCGCCAC CATGGTGAGC 121 AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA 181 AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG 241 ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC 301 ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC 361 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC 421 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC 481 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG 541 TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG 601 GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC 661 CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC 721 ACCCAGTCCA AGCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG 781 TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGTC CGGACTCAGA 841 TCTGTGAAAA GCGAAGGCAA ACGCAAAGGC GGCGAAGTGG CCAAAAAAGA AGAGCAAGAA 901 AGAAAAGGAT AAAGATAGCA AACTGGGAAA AAGCGCTGAA AGCCGAAAGC TTCGAAATTC 961 TGCAGTCGAC GGGTACCGCG GGGCCCGGGG ATCCCACCCG GGATCTAGAT AACTGATCAT 1021 AATCAGCCCA TACCAACATT TTGGTAGAGG GTTTTACTTT GCTTTAAAAA AAAACTTCCC 1081 ACACGTTCCC CTCCCTTGGA ACTTGCAAAT CCATTCAAAT GATGCTATTG CATGCCTGGG 1141 CTA // |
| Region: NeoR_KanR | Primer: SV40pro_F_V2 | Sequence: RDB15741_A8Anc.seq |
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>D05001A3-4_A8An_SV40pro_F_V2_H02_23_ABI24.ab1 1 GGGCAGTTTT TTGGAGGGCT AGGCTTTTGC AAGATCGATC AAGAGACAGG ATGAGGATCG 61 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG 121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG 181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT 241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA 301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG 361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT 421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA 481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG 541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG 601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG 661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT 721 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC 781 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC 841 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC 901 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG 961 GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCCTCCAGC GCGGGGGATC TCATGCTGGG 1021 AGTTCTTTCG CCCACCCTAG GGGGGAGGCT ACTGGAAACA CGGAAGGAGA CCATTACCCG 1081 AAGACCCCGC CGCTATTGAC GGCCAATTA // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
| original | Kitamura, A., Efficient and dynamic nuclear localization of green fluorescent protein via RNA binding. Biochem. Biophys. Res. Commun. 463 (3): 401-406 (2015). PMID 26032495. |
|---|
2019.09.02
GNP_filter3_RDBDEP_html_190816.pl