Please review the QC test results indicated by icon below as well as clone information before placing your order.

GFP-NLS PARP

Fluorescent nuclear marker, broader distribution in nucleus and leaky in cytoplasm.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kitamura, A. et al., Biochem. Biophys. Res. Commun., 463 (3): 401-406, 2015).

Ordering

Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kitamura, A. et al., Biochem. Biophys. Res. Commun., 463 (3): 401-406, 2015)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします （Schedule AとSchedule Bを入手[link])。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15741_A8Anp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40polyA_R2 Region: Sv40p(A),PARP NLS,mEGFP Sequence file: RDB15741_A8Ana.seq
>D05001A3_A8An_4_SV40polyA-R2_C02_03_ABI08.ab1     1 GCAAATTAAT AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA    61 GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT   121 GGCTGATTAT GATCAGTTAT CTAGATCCGG TGGATCCCGG GCCCGCGGTA CCGTCGACTG   181 CAGAATTCGA AGCTTCGGCT TTCAGCGCTT TTTCCAGTTT GCTATCTTTA TCCTTTTCTT   241 TCTTGCTCTT CTTTTTGGCC ACTTCGCCGC CTTTGCGTTT GCCTTCGCTT TTCACAGATC   301 TGAGTCCGGA CTTGTACAGC TCGTCCATGC CGAGAGTGAT CCCGGCGGCG GTCACGAACT   361 CCAGCAGGAC CATGTGATCG CGCTTCTCGT TGGGGTCTTT GCTCAGCTTG GACTGGGTGC   421 TCAGGTAGTG GTTGTCGGGC AGCAGCACGG GGCCGTCGCC GATGGGGGTG TTCTGCTGGT   481 AGTGGTCGGC GAGCTGCACG CTGCCGTCCT CGATGTTGTG GCGGATCTTG AAGTTCACCT   541 TGATGCCGTT CTTCTGCTTG TCGGCCATGA TATAGACGTT GTGGCTGTTG TAGTTGTACT   601 CCAGCTTGTG CCCCAGGATG TTGCCGTCCT CCTTGAAGTC GATGCCCTTC AGCTCGATGC   661 GGTTCACCAG GGTGTCGCCC TCGAACTTCA CCTCGGCGCG GGTCTTGTAG TTGCCGTCGT   721 CCTTGAAGAA GATGGTGCGC TCCTGGACGT AGCCTTCGGG CATGGCGGAC TTGAAGAAGT   781 CGTGCTGCTT CATGTGGTCG GGGTAGCGGC TGAAGCACTG CACGCCGTAG GTCAGGGTGG   841 TCACGAGGGT GGGCCAGGGC ACGGGCAGCT TGCCGGTGGT GCAGATGAAC TTCAGGGTCA   901 GCTTGCGTAG TGGCATCGCC CTCGCCCTCG CCGGACAACG CTGAACTTGT GGCCGTTTAC   961 CGTCGCCGTC CAACTCGACC AGGATGGGCA ACACCCCGGT GACAGCTCTC GCCCTTGGCT  1021 CACCATGGTG GCGACCGGGT ACCGCTTACC GGATCTGTAC GGTTCACTAA ACCCAGCTCC  1081 TGCTTATATA TGACCTCCCA CCCGTTCACG CCTAACCGCC CCAATTTGCT TTCAATGGGG  1141 GCGGAAGTTG TTTCGAAACA TTTTGGAAGT CCCCGTTTGA TTTTGGGTGG CCAAAATCAA 1201 CCTTCCAATT GAACGTCTTA TGGGGGTTGG TAGAGCTTTG GGGAATACTC CGCCTCG //
Primer: CMV_Forward Region: CMV pro,mEGFP,PARP NLS Sequence file: RDB15741_A8Anb.seq
>D05001A3-4_A8An_CMV-Forward_G02_20_ABI24.ab1     1 CATCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG    61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGTCGCCAC CATGGTGAGC   121 AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA   181 AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG   241 ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC   301 ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC   361 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC   421 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC   481 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG   541 TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG   601 GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC   661 CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC   721 ACCCAGTCCA AGCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG   781 TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGTC CGGACTCAGA   841 TCTGTGAAAA GCGAAGGCAA ACGCAAAGGC GGCGAAGTGG CCAAAAAAGA AGAGCAAGAA   901 AGAAAAGGAT AAAGATAGCA AACTGGGAAA AAGCGCTGAA AGCCGAAAGC TTCGAAATTC   961 TGCAGTCGAC GGGTACCGCG GGGCCCGGGG ATCCCACCCG GGATCTAGAT AACTGATCAT  1021 AATCAGCCCA TACCAACATT TTGGTAGAGG GTTTTACTTT GCTTTAAAAA AAAACTTCCC  1081 ACACGTTCCC CTCCCTTGGA ACTTGCAAAT CCATTCAAAT GATGCTATTG CATGCCTGGG 1141 CTA //
Primer: SV40pro_F_V2 Region: NeoR_KanR Sequence file: RDB15741_A8Anc.seq
>D05001A3-4_A8An_SV40pro_F_V2_H02_23_ABI24.ab1     1 GGGCAGTTTT TTGGAGGGCT AGGCTTTTGC AAGATCGATC AAGAGACAGG ATGAGGATCG    61 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG   121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG   181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT   241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA   301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG   361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT   421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA   481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG   541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG   601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG   661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT   721 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC   781 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC   841 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC   901 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG   961 GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCCTCCAGC GCGGGGGATC TCATGCTGGG  1021 AGTTCTTTCG CCCACCCTAG GGGGGAGGCT ACTGGAAACA CGGAAGGAGA CCATTACCCG 1081 AAGACCCCGC CGCTATTGAC GGCCAATTA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.07.13

GNP_filter3_RDBDEP_html_220713.pl