RDB15741 - GFP-NLS PARP

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GFP-NLS PARP

Fluorescent nuclear marker, broader distribution in nucleus and leaky in cytoplasm.

Catalog number	RDB15741
Resource name	GFP-NLS PARP
Clone info.	Fluorescent nuclear marker, broader distribution in nucleus and leaky in cytoplasm: expression vector of GFP, tagged with a single NLS sequence of poly (ADP-ribose) polymerase (PARP) at C-terminal.
Comment	Expression was confirmed by the depositor with microscopic observation. Utilized EGFP with monomeric mutation (A206K, D.A. Zacharias et al., Science. 296 (5569): 913-916, 2002).
Vector backbone	pEGFP-C1 (plasmid)
Gene/insert name	cDNA
Depositor\|Developer	Kinjo, Masataka \|

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External Database

Reference sequence

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kitamura, A. et al., Biochem. Biophys. Res. Commun., 463 (3): 401-406, 2015).

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Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kitamura, A. et al., Biochem. Biophys. Res. Commun., 463 (3): 401-406, 2015).
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kitamura, A. et al., Biochem. Biophys. Res. Commun., 463 (3): 401-406, 2015)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15741	GFP-NLS PARP	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

References and tips

Original reference

original	Kitamura, A., Efficient and dynamic nuclear localization of green fluorescent protein via RNA binding. Biochem. Biophys. Res. Commun. 463 (3): 401-406 (2015). PMID 26032495.

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15741_A8Anp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40polyA_R2 () Region: Sv40p(A),PARP NLS,mEGFP Sequence file: RDB15741_A8Ana.seq
>D05001A3_A8An_4_SV40polyA-R2_C02_03_ABI08.ab1 1 GCAAATTAAT AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA 61 GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT 121 GGCTGATTAT GATCAGTTAT CTAGATCCGG TGGATCCCGG GCCCGCGGTA CCGTCGACTG 181 CAGAATTCGA AGCTTCGGCT TTCAGCGCTT TTTCCAGTTT GCTATCTTTA TCCTTTTCTT 241 TCTTGCTCTT CTTTTTGGCC ACTTCGCCGC CTTTGCGTTT GCCTTCGCTT TTCACAGATC 301 TGAGTCCGGA CTTGTACAGC TCGTCCATGC CGAGAGTGAT CCCGGCGGCG GTCACGAACT 361 CCAGCAGGAC CATGTGATCG CGCTTCTCGT TGGGGTCTTT GCTCAGCTTG GACTGGGTGC 421 TCAGGTAGTG GTTGTCGGGC AGCAGCACGG GGCCGTCGCC GATGGGGGTG TTCTGCTGGT 481 AGTGGTCGGC GAGCTGCACG CTGCCGTCCT CGATGTTGTG GCGGATCTTG AAGTTCACCT 541 TGATGCCGTT CTTCTGCTTG TCGGCCATGA TATAGACGTT GTGGCTGTTG TAGTTGTACT 601 CCAGCTTGTG CCCCAGGATG TTGCCGTCCT CCTTGAAGTC GATGCCCTTC AGCTCGATGC 661 GGTTCACCAG GGTGTCGCCC TCGAACTTCA CCTCGGCGCG GGTCTTGTAG TTGCCGTCGT 721 CCTTGAAGAA GATGGTGCGC TCCTGGACGT AGCCTTCGGG CATGGCGGAC TTGAAGAAGT 781 CGTGCTGCTT CATGTGGTCG GGGTAGCGGC TGAAGCACTG CACGCCGTAG GTCAGGGTGG 841 TCACGAGGGT GGGCCAGGGC ACGGGCAGCT TGCCGGTGGT GCAGATGAAC TTCAGGGTCA 901 GCTTGCGTAG TGGCATCGCC CTCGCCCTCG CCGGACAACG CTGAACTTGT GGCCGTTTAC 961 CGTCGCCGTC CAACTCGACC AGGATGGGCA ACACCCCGGT GACAGCTCTC GCCCTTGGCT 1021 CACCATGGTG GCGACCGGGT ACCGCTTACC GGATCTGTAC GGTTCACTAA ACCCAGCTCC 1081 TGCTTATATA TGACCTCCCA CCCGTTCACG CCTAACCGCC CCAATTTGCT TTCAATGGGG 1141 GCGGAAGTTG TTTCGAAACA TTTTGGAAGT CCCCGTTTGA TTTTGGGTGG CCAAAATCAA 1201 CCTTCCAATT GAACGTCTTA TGGGGGTTGG TAGAGCTTTG GGGAATACTC CGCCTCG //
Primer: CMV_Forward () Region: CMV pro,mEGFP,PARP NLS Sequence file: RDB15741_A8Anb.seq
>D05001A3-4_A8An_CMV-Forward_G02_20_ABI24.ab1 1 CATCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG 61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGTCGCCAC CATGGTGAGC 121 AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA 181 AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG 241 ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC 301 ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC 361 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC 421 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC 481 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG 541 TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG 601 GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC 661 CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC 721 ACCCAGTCCA AGCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG 781 TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGTC CGGACTCAGA 841 TCTGTGAAAA GCGAAGGCAA ACGCAAAGGC GGCGAAGTGG CCAAAAAAGA AGAGCAAGAA 901 AGAAAAGGAT AAAGATAGCA AACTGGGAAA AAGCGCTGAA AGCCGAAAGC TTCGAAATTC 961 TGCAGTCGAC GGGTACCGCG GGGCCCGGGG ATCCCACCCG GGATCTAGAT AACTGATCAT 1021 AATCAGCCCA TACCAACATT TTGGTAGAGG GTTTTACTTT GCTTTAAAAA AAAACTTCCC 1081 ACACGTTCCC CTCCCTTGGA ACTTGCAAAT CCATTCAAAT GATGCTATTG CATGCCTGGG 1141 CTA //
Primer: SV40pro_F_V2 () Region: NeoR_KanR Sequence file: RDB15741_A8Anc.seq
>D05001A3-4_A8An_SV40pro_F_V2_H02_23_ABI24.ab1 1 GGGCAGTTTT TTGGAGGGCT AGGCTTTTGC AAGATCGATC AAGAGACAGG ATGAGGATCG 61 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG 121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG 181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT 241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA 301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG 361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT 421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA 481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG 541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG 601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG 661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT 721 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC 781 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC 841 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC 901 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG 961 GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCCTCCAGC GCGGGGGATC TCATGCTGGG 1021 AGTTCTTTCG CCCACCCTAG GGGGGAGGCT ACTGGAAACA CGGAAGGAGA CCATTACCCG 1081 AAGACCCCGC CGCTATTGAC GGCCAATTA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Kitamura, A., Efficient and dynamic nuclear localization of green fluorescent protein via RNA binding. Biochem. Biophys. Res. Commun. 463 (3): 401-406 (2015). PMID 26032495.

2023.04.06

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