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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

GFP-NLS PARP

Fluorescent nuclear marker, broader distribution in nucleus and leaky in cytoplasm.

Catalog number RDB15741
Resource name GFP-NLS PARP
Clone info. Fluorescent nuclear marker, broader distribution in nucleus and leaky in cytoplasm: expression vector of GFP, tagged with a single NLS sequence of poly (ADP-ribose) polymerase (PARP) at C-terminal.
Comment Expression was confirmed by the depositor with microscopic observation. Utilized EGFP with monomeric mutation (A206K, D.A. Zacharias et al., Science. 296 (5569): 913-916, 2002).
Vector backbone pEGFP-C1 (plasmid)
Gene/insert name cDNA
Depositor|Developer Kinjo, Masataka |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kitamura, A. et al., Biochem. Biophys. Res. Commun., 463 (3): 401-406, 2015).
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kitamura, A. et al., Biochem. Biophys. Res. Commun., 463 (3): 401-406, 2015)。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB15741 GFP-NLS PARP DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Kitamura, A., Efficient and dynamic nuclear localization of green fluorescent protein via RNA binding. Biochem. Biophys. Res. Commun. 463 (3): 401-406 (2015). PMID 26032495.

            Further references such as user reports and related articles (go to bottom)

            Featured content


            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15741_A8Anp1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: SV40polyA_R2
            Region: Sv40p(A),PARP NLS,mEGFP
            Sequence file: RDB15741_A8Ana.seq check
            >D05001A3_A8An_4_SV40polyA-R2_C02_03_ABI08.ab1
                1 GCAAATTAAT AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA
               61 GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT
              121 GGCTGATTAT GATCAGTTAT CTAGATCCGG TGGATCCCGG GCCCGCGGTA CCGTCGACTG
              181 CAGAATTCGA AGCTTCGGCT TTCAGCGCTT TTTCCAGTTT GCTATCTTTA TCCTTTTCTT
              241 TCTTGCTCTT CTTTTTGGCC ACTTCGCCGC CTTTGCGTTT GCCTTCGCTT TTCACAGATC
              301 TGAGTCCGGA CTTGTACAGC TCGTCCATGC CGAGAGTGAT CCCGGCGGCG GTCACGAACT
              361 CCAGCAGGAC CATGTGATCG CGCTTCTCGT TGGGGTCTTT GCTCAGCTTG GACTGGGTGC
              421 TCAGGTAGTG GTTGTCGGGC AGCAGCACGG GGCCGTCGCC GATGGGGGTG TTCTGCTGGT
              481 AGTGGTCGGC GAGCTGCACG CTGCCGTCCT CGATGTTGTG GCGGATCTTG AAGTTCACCT
              541 TGATGCCGTT CTTCTGCTTG TCGGCCATGA TATAGACGTT GTGGCTGTTG TAGTTGTACT
              601 CCAGCTTGTG CCCCAGGATG TTGCCGTCCT CCTTGAAGTC GATGCCCTTC AGCTCGATGC
              661 GGTTCACCAG GGTGTCGCCC TCGAACTTCA CCTCGGCGCG GGTCTTGTAG TTGCCGTCGT
              721 CCTTGAAGAA GATGGTGCGC TCCTGGACGT AGCCTTCGGG CATGGCGGAC TTGAAGAAGT
              781 CGTGCTGCTT CATGTGGTCG GGGTAGCGGC TGAAGCACTG CACGCCGTAG GTCAGGGTGG
              841 TCACGAGGGT GGGCCAGGGC ACGGGCAGCT TGCCGGTGGT GCAGATGAAC TTCAGGGTCA
              901 GCTTGCGTAG TGGCATCGCC CTCGCCCTCG CCGGACAACG CTGAACTTGT GGCCGTTTAC
              961 CGTCGCCGTC CAACTCGACC AGGATGGGCA ACACCCCGGT GACAGCTCTC GCCCTTGGCT
             1021 CACCATGGTG GCGACCGGGT ACCGCTTACC GGATCTGTAC GGTTCACTAA ACCCAGCTCC
             1081 TGCTTATATA TGACCTCCCA CCCGTTCACG CCTAACCGCC CCAATTTGCT TTCAATGGGG
             1141 GCGGAAGTTG TTTCGAAACA TTTTGGAAGT CCCCGTTTGA TTTTGGGTGG CCAAAATCAA
            1201 CCTTCCAATT GAACGTCTTA TGGGGGTTGG TAGAGCTTTG GGGAATACTC CGCCTCG
            //
            Primer: CMV_Forward
            Region: CMV pro,mEGFP,PARP NLS
            Sequence file: RDB15741_A8Anb.seq check
            >D05001A3-4_A8An_CMV-Forward_G02_20_ABI24.ab1
                1 CATCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
               61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGTCGCCAC CATGGTGAGC
              121 AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA
              181 AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG
              241 ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC
              301 ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC
              361 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC
              421 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC
              481 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG
              541 TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG
              601 GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC
              661 CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC
              721 ACCCAGTCCA AGCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG
              781 TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGTC CGGACTCAGA
              841 TCTGTGAAAA GCGAAGGCAA ACGCAAAGGC GGCGAAGTGG CCAAAAAAGA AGAGCAAGAA
              901 AGAAAAGGAT AAAGATAGCA AACTGGGAAA AAGCGCTGAA AGCCGAAAGC TTCGAAATTC
              961 TGCAGTCGAC GGGTACCGCG GGGCCCGGGG ATCCCACCCG GGATCTAGAT AACTGATCAT
             1021 AATCAGCCCA TACCAACATT TTGGTAGAGG GTTTTACTTT GCTTTAAAAA AAAACTTCCC
             1081 ACACGTTCCC CTCCCTTGGA ACTTGCAAAT CCATTCAAAT GATGCTATTG CATGCCTGGG
            1141 CTA
            //
            Primer: SV40pro_F_V2
            Region: NeoR_KanR
            Sequence file: RDB15741_A8Anc.seq check
            >D05001A3-4_A8An_SV40pro_F_V2_H02_23_ABI24.ab1
                1 GGGCAGTTTT TTGGAGGGCT AGGCTTTTGC AAGATCGATC AAGAGACAGG ATGAGGATCG
               61 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
              121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
              181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
              241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
              301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
              361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
              421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
              481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
              541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG
              601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
              661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT
              721 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC
              781 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC
              841 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC
              901 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG
              961 GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCCTCCAGC GCGGGGGATC TCATGCTGGG
             1021 AGTTCTTTCG CCCACCCTAG GGGGGAGGCT ACTGGAAACA CGGAAGGAGA CCATTACCCG
            1081 AAGACCCCGC CGCTATTGAC GGCCAATTA
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Kitamura, A., Efficient and dynamic nuclear localization of green fluorescent protein via RNA binding. Biochem. Biophys. Res. Commun. 463 (3): 401-406 (2015). PMID 26032495.

            2022.07.13

            GNP_filter3_RDBDEP_html_220713.pl