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GFP-NLS SV40 (#RDB15740)

Fluorescent nuclear marker accumulated not only in the nucleoplasm but also in the nucleolus.

Clone info. Fluorescent nuclear marker accumulated not only in the nucleoplasm but also in the nucleolus: expression vector of GFP tagged with three tandem repeats of NLS derived from Simian virus 40 (SV40) at C-terminal.
Comment Expression was confirmed by the depositor with microscopic observation. Utilized EGFP with monomeric mutation (A206K, D.A. Zacharias et al., Science. 296 (5569): 913-916, 2002).
Vector backbone pEGFP-C1 (plasmid)
Gene/insert name cDNA
Depositor|Developer Kinjo, Masataka |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kitamura, A. et al., Biochem. Biophys. Res. Commun., 463 (3): 401-406, 2015).
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kitamura, A. et al., Biochem. Biophys. Res. Commun., 463 (3): 401-406, 2015)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB15740 GFP-NLS SV40 Under QC test. Please contact us. DNA solution

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How to cite this biological resource

Materials & Methods section:

The GFP-NLS SV40 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15740).

Reference section:

Kitamura, A., Nakayama, Y., Kinjo, M., Efficient and dynamic nuclear localization of green fluorescent protein via RNA binding. Biochem. Biophys. Res. Commun. 463 (3): 401-406 (2015). PMID 26032495. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Kitamura, A., Efficient and dynamic nuclear localization of green fluorescent protein via RNA binding. Biochem. Biophys. Res. Commun. 463 (3): 401-406 (2015). PMID 26032495. [link to RRC of NBRP]