Fluorescent nuclear marker accumulated not only in the nucleoplasm but also in the nucleolus.
| Catalog number | RDB15740 |
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| Resource name | GFP-NLS SV40 |
| Clone info. | Fluorescent nuclear marker accumulated not only in the nucleoplasm but also in the nucleolus: expression vector of GFP tagged with three tandem repeats of NLS derived from Simian virus 40 (SV40) at C-terminal. Expression was confirmed by the depositor with microscopic observation. Utilized EGFP with monomeric mutation (A206K, D.A. Zacharias et al., Science. 296 (5569): 913-916, 2002). |
| Vector backbone | pEGFP-C1 (plasmid) |
| Gene/insert name | cDNA |
| Depositor | Kinjo, Masataka | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kitamura, A. et al., Biochem. Biophys. Res. Commun., 463 (3): 401-406, 2015). |
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| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link] |
| Remarks | Please use Schedule A (GFP-A) additionally. |
[open/close]| Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
|---|---|---|---|---|
| RDB15740 | GFP-NLS SV40 | Under QC test. Please contact us. | DNA solution |
Electronic file
Original reference
| original | Kitamura, A., Efficient and dynamic nuclear localization of green fluorescent protein via RNA binding. Biochem. Biophys. Res. Commun. 463 (3): 401-406 (2015). PMID 26032495. |
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Further references such as user reports and related articles (go to bottom)
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This clone will be sequenced a portion and digested by restriction enzyme for examination.
Original, user report and related articles
| original | Kitamura, A., Efficient and dynamic nuclear localization of green fluorescent protein via RNA binding. Biochem. Biophys. Res. Commun. 463 (3): 401-406 (2015). PMID 26032495. |
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2019.07.17
GNP_filter3_RDBDEP_html_190432.pl