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GFP-NLS SV40

Fluorescent nuclear marker accumulated not only in the nucleoplasm but also in the nucleolus.

Catalog number RDB15740
Resource name GFP-NLS SV40
Clone info. Fluorescent nuclear marker accumulated not only in the nucleoplasm but also in the nucleolus: expression vector of GFP tagged with three tandem repeats of NLS derived from Simian virus 40 (SV40) at C-terminal.
Expression was confirmed by the depositor with microscopic observation. Utilized EGFP with monomeric mutation (A206K, D.A. Zacharias et al., Science. 296 (5569): 913-916, 2002).
Vector backbone pEGFP-C1 (plasmid)
Gene/insert name cDNA
Depositor Kinjo, Masataka |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kitamura, A. et al., Biochem. Biophys. Res. Commun., 463 (3): 401-406, 2015).
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Schedule A (GFP-A) [Link]
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB15740 GFP-NLS SV40 Under QC test. Please contact us. DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Kitamura, A., Efficient and dynamic nuclear localization of green fluorescent protein via RNA binding. Biochem. Biophys. Res. Commun. 463 (3): 401-406 (2015). PMID 26032495.

Further references such as user reports and related articles (go to bottom)

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Kitamura, A., Efficient and dynamic nuclear localization of green fluorescent protein via RNA binding. Biochem. Biophys. Res. Commun. 463 (3): 401-406 (2015). PMID 26032495.

2019.07.17

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