Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pSIX3G137C (#RDB15722)

Plasmid clone of G137C mutant of secreted in xylem3 (SIX3) gene of Fusarium oxysporum f. sp. lycopersici (Fol).

Clone info. Plasmid clone of G137C mutant of secreted in xylem3 (SIX3) gene of Fusarium oxysporum f. sp. lycopersici (Fol). This clone was used in the loop-mediated isothermal amplification (LAMP) assay.
Comment Wild-type and variants mentioned in Ayukawa, Y. et al., Sci. Rep., 7 (1): 4253, 2017 can be acquired from the natural environment. GenBank: AM234063.1
Vector backbone pGEM-T easy (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Fusarium oxysporum f. sp. lycopersici SIX3
Depositor|Developer Komatsu, Ken |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution For profit-use of the BIOLOGICAL RESOURCE, the RECIPIENT must obtain a prior written consent from The University Research Administration Center, Tokyo University of Agriculture and Technology ( suishin@ml.tuat.ac.jp ). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature and state an acknowledgement to the DEPOSITOR. (Ayukawa, Y et al. Sci. Rep. 7(1): 4253, 2017)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 営利目的利用の希望については、希望者は予め農工大研究推進センターに連絡し、承諾を得る。利用者は、研究成果の公表にあたって寄託者の指定する文献の引用と、寄託者への謝辞の表明を必要とする。 (Ayukawa, Y et al. Sci. Rep. 7(1): 4253, 2017)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15722 pSIX3G137C DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pSIX3G137C was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15722).

Reference section:

Ayukawa, Y., Hanyuda, S., Fujita, N., Komatsu, K., Arie, T., Novel loop-mediated isothermal amplification (LAMP) assay with a universal QProbe can detect SNPs determining races in plant pathogenic fungi. Sci. Rep. 7 (1): 4253 (2017). PMID 28652587. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15722_A8A9p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40
Sequence file: RDB15722_A8A9a.seq check
>D05148A1-1_A8A9_M13_-40_E01_13_ABI24.ab1
    1 GGCAGAGGTG AAATTGTAAT ACGGACTCAC TATAGGGCGA ATTGGGCCCG ACGTCGCATG
   61 CTCCCGGCCG CCATGGCGGC CGCGGGAATT CGATTTATAT TACCGACCAT CTTGCCTAAA
  121 CATTTACCAC TATGCGTTTC CTTCTGCTTA TAGCCATGTC CATGACATGG GTTTGCTCTA
  181 TTGCTGGGCT ACCTGTGGAA GATGCCGATT CATCTGTCGG TCAGCTCCAA GGACGGGGTA
  241 ACCCATATTG CGTGTTTCCC GGCCGCCCCA CGTCTTCTAC TTCATTTACT ACGAGCTTCA
  301 GCACCGAACC TCTGGGTTAT GCTAGAATGT TACACAGAGA CCCTCCATAT GAGCGGGCTG
  361 GCAATTCGGG ACTGAATCAC CGCATTTACG AACGAAGTCG CGTTGGAGGC CTCCGCACTG
  421 TCATTGATGT CGCCCCCCCG GACGGACACC AAGCCATCGC CAACTACGAG ATCGAAGTCC
  481 GTCGTATTCC TGTTGCAACA CCTAATGCGG CTGGAGATTG CTTTCACACT GCACGCCTTA
  541 GTACCGGCTC TCGAGGTCCA GCCACAATCA GTTGGGATGC CGACGCTAGT TATACCTACT
  601 ATCTTACTAT CTCAGAGGAT TGATTCTGTG GCAGTTCCCC TTGGCAATCA CTAGTGAATT
  661 CGCGGCCGCC TGCAGGTCGA CCATATGGGA GAGCTCCCAA CGCGTTGGAT GCATAGCTTG
  721 AGTATTCTAT AGTGTCACCT AAATAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG
  781 TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA
  841 GCCTGGGGTG CCTAATGGAG TGGAGCTAAC TCACATTAAA TGCCGTTGCG CCTCCACTGC
  901 CCGCTTTCCA GTCGGGAAAA CTTGTCCGTG CCAGCCTGCA TAATTGAATC GGCACCGCCG
  961 GGAGAAGGCG TGCGATATTG GGGGCCTTTC CTGTTCCTCG TCCATGAATC CTTGCCTCGG
 1021 CTTCCGTGCG CGAAGGTACG ATCACTAGCG GTATCGGTAC CAATCGATAC GAAACTGTGC
1081 AACGG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ayukawa, Y., Novel loop-mediated isothermal amplification (LAMP) assay with a universal QProbe can detect SNPs determining races in plant pathogenic fungi. Sci. Rep. 7 (1): 4253 (2017). PMID 28652587. [link to RRC of NBRP]