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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

mIL-6(-300_-79)-luc (#RDB15721)

Reporter plasmid of mouse interleukin 6 (IL-6) promoter fragment (nt -300 to -79).

Alternative name mouse IL-6 promoter fragment (nt -300 to -79)/pGL4
Clone info. Reporter plasmid of mouse interleukin 6 (IL-6) promoter fragment (nt -300 to -79) fused with C-terminal Luciferase, translation initiation site is numbered as +1.
Comment Expression was confirmed by the depositor with luciferase reporter assay.
Vector backbone pGL4 (plasmid)
Size of vector backbone 4.2 kb
Selectable markers Amp^r
Gene/insert name mouse Il6 genomic
Depositor|Developer Murakami, Masaru |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kanamori, Y et al. J. Biol. Chem. 292(24):10275-10287, 2017)
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kanamori, Y et al. J. Biol. Chem. 292(24):10275-10287, 2017)。
MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15721 mIL-6(-300_-79)-luc DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The mIL-6(-300_-79)-luc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15721).

Reference section:

Kanamori, Y., Murakami, M., Sugiyama, M., Hashimoto, O., Matsui, T., Funaba, M., Interleukin-1β (IL-1β) transcriptionally activates hepcidin by inducing CCAAT enhancer-binding protein δ (C/EBPδ) expression in hepatocytes. J. Biol. Chem. 292 (24): 10275-10287 (2017). PMID 28438835. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15721_A7Lpp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F ()
Sequence file: RDB15721_A7Lpa.seq check
>D05137A2_A7Lp_1_pGL4-4174F_D05_11_ABI24.ab1
    1 ATGGGTCAGT ACTTTCTCTG GCCTAACTGG CCGGTACCGA GTGCTCATGC TTCTTAGGGC
   61 TAGCCTCAAG GATGACTTAA GCACACTTTC CCCTTCCTAG TTGTGATTCT TTCGATGCTA
  121 AACGACGTCA CATTGTGCAA TCTTAATAAG GTTTCCAATC AGCCCCACCC ACTCTGGCCC
  181 CACCCCCACC CTCCAACAAA GATTTTTATC AAATGTGGGA TTTTCCCATG AGTCTCAAAA
  241 TTAGAGAGTT GACTCCTAAT AAGCTTGGCA ATCCGGTACT GTTGGTAAAG CCACCATGGA
  301 AGATGCCAAA AACATTAAGA AGGGCCCAGC GCCATTCTAC CCACTCGAAG ACGGGACCGC
  361 CGGCGAGCAG CTGCACAAAG CCATGAAGCG CTACGCCCTG GTGCCCGGCA CCATCGCCTT
  421 TACCGACGCA CATATCGAGG TGGACATTAC CTACGCCGAG TACTTCGAGA TGAGCGTTCG
  481 GCTGGCAGAA GCTATGAAGC GCTATGGGCT GAATACAAAC CATCGGATCG TGGTGTGCAG
  541 CGAGAATAGC TTGCAGTTCT TCATGCCCGT GTTGGGTGCC CTGTTCATCG GTGTGGCTGT
  601 GGCCCCAGCT AACGACATCT ACAACGAGCG CGAGCTGCTG AACAGCATGG GCATCAGCCA
  661 GCCCACCGTC GTATTCGTGA GCAAGAAAGG GCTGCAAAAG ATCCTCAACG TGCAAAAGAA
  721 GCTACCGATC ATACAAAAGA TCATCATCAT GGATAGCAAG ACCGACTACC AGGGCTTCCA
  781 AAGCATGTAC ACCTTCGTGA CTTCCCATTT GCCACCCGGC TTCAACGAGT ACGACTTCGT
  841 GCCCGAGAGC TTCGACCGGG GACAAAACCA TCGCCCTGAT CATGAACAGT AGTGGCAGTA
  901 CCGGATTGCC CAAAGGGCGT AGCCCTACCG CACCGCACCG CTTGTGTCCG ATTCAGTCAT
  961 GCCCGCGACC CCATCTTCGG CAACCAGATC ATCCCCCGAC ACCGCTATCC TCAGCGTGGT
 1021 GCCCATTTCA CCACGGCTTT CGCATGTTTC ACCACGCTGG GCCTACTTTG ATCTGCGGCT
 1081 TTCGGGTTCC ATGCTTCATG GTAACGGCTT CCGAGAGAGC TTAATTCTTG GCCGCCAAGC
1141 CATC
//
Primer: pGCAP_R ()
Sequence file: RDB15721_A7Lpb.seq check
>D05137A2_A7Lp_1_pGCAP_R_E05_14_ABI24.ab1
    1 ACAACATATC ATGTCTGCTC GAGCGGCCGG CCGCCCCGAC TCTAGAATTA TTACACGGCG
   61 ATCTTGCCGC CCTTCTTGGC CTTAATGAGA ATCTCGCGGA TCTTGCGGGC GTCCAACTTG
  121 CCGGTCAGTC CTTTAGGCAC CTCGTCCACG AACACAACAC CACCGCGCAG CTTCTTGGCG
  181 GTTGTAACCT GGCTGGCCAC ATAGTCCACG ATCTCCTTCT CGGTCATGGT TTTACCGTGT
  241 TCCAGCACGA CGACTGCGGC GGGCAGCTCG CCGGCATCGT CGTCGGGCAG GCCGGCGACC
  301 CCGGCGTCGA AGATGTTGGG GTGTTGCAGC AGGATGCTCT CCAGTTCGGC TGGGGCTACC
  361 TGGTAGCCCT TGTATTTGAT CAGGCTCTTC AGCCGGTCCA CGATGAAGAA GTGCTCGTCC
  421 TCGTCCCAGT AGGCGATGTC GCCGCTGTGC AGCCAGCCGT CCTTGTCGAT GAGAGCGTTT
  481 GTAGCCTCGG GGTTGTTAAC GTAGCCGCTC ATGATCATGG GGCCACGGAC GCACAGCTCG
  541 CCGCGCTGGT TCACACCCAG TGTCTTACCG GTGTCCAAGT CCACCACCTT AGCCTCGAAG
  601 AAGGGCACCA CCTTGCCTAC TGCGCCAGGC TTGTCGTCCC CTTCGGGGGT GATCAGAATG
  661 GCGCTGGTTG TTTCTGTCAG GCCGTAGCCC TGGCGGATGC CTGGTAGGTG GAAGCGTTTG
  721 GCCACGGCCT CACCTACCTC CTTGCTGAGC GGCGCCCCGC CGCTGGCGAT CTCGTGCAAG
  781 TTGCTTAGGT CGTACTTGTC GATGAGAGTG CTCTTAGCGA AGAAGCTAAA TAGTGTGGGC
  841 ACCAGCAGGG CAGATTGAAT CTTATAGTCT TGCAAGCTGC GCAAGAATAG CTCCTCCTCG
  901 AAGCGGTACA TGAGCACGAC CCGAAAGCCG CAGATCAAGT AGCCCAGCGT GGTGAACATG
  961 CCGAAGCCGT GGGTGAAATG GCACCACGCT GAGGATAGCG GTGTCGGGGA TGATCTGGGT
 1021 TTGCCGAGAT GGGGGTCCGC CGGGCCATGA ACTGATCGGA CACAGGCGGG TGCCGTTGCG
1081 GTTAAGGGCT ACGCCCTTGG GCCAATCCGG TTACTGGC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kanamori, Y., Interleukin-1β (IL-1β) transcriptionally activates hepcidin by inducing CCAAT enhancer-binding protein δ (C/EBPδ) expression in hepatocytes. J. Biol. Chem. 292 (24): 10275-10287 (2017). PMID 28438835. [link to RRC of NBRP]