RDB15720 - rHpcidin(-1861)-luc

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rHpcidin(-1861)-luc

Reporter plasmid of rat hepcidin antimicrobial peptide (Hamp) promoter fragment (nt -1861 to -35).

Catalog number	RDB15720
Resource name	rHpcidin(-1861)-luc
Alternative name	rhepcidin-luc
Clone info.	Reporter plasmid of rat hepcidin antimicrobial peptide (Hamp) promoter fragment (nt -1861 to -35) fused with C-terminal Luciferase, translation initiation site is numbered as +1.
Comment	Expression was confirmed by the depositor with luciferase reporter assay.
Vector backbone	pGL4 (plasmid)
Size of vector backbone	4.2 kb
Selectable markers	Amp^r
Gene/insert name	rat Hamp genomic
Depositor\|Developer	Murakami, Masaru \|

Other clones in our bank

External Database
rat Hamp

NCBI Gene

Reference sequence

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kanamori, Y et al. J. Biol. Chem. 292(24):10275-10287, 2017)

Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kanamori, Y et al. J. Biol. Chem. 292(24):10275-10287, 2017)
Additional terms and conditions for distribution	The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kanamori, Y et al. J. Biol. Chem. 292(24):10275-10287, 2017)。
付加的提供条件	本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15720	rHpcidin(-1861)-luc	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original	Kanamori, Y., Interleukin-1β (IL-1β) transcriptionally activates hepcidin by inducing CCAAT enhancer-binding protein δ (C/EBPδ) expression in hepatocytes. J. Biol. Chem. 292 (24): 10275-10287 (2017). PMID 28438835.

Further references such as user reports and related articles (go to bottom)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15720_A7Lpp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F Sequence file: RDB15720_A7Lpa.seq
>D05137A1_A7Lp_1_pGL4-4174F_A05_02_ABI24.ab1 1 ATGGGGTCAG ACATTTCTCT GGCCTAACTG GCCGGTACCC CGCCTTGTCT GTCTGTGTGT 61 CCATGTTGGG CATGGGGTGG GGAAAGAGTC CTAGGGTCCT CTTCAGGTCT AAGCTTAAGT 121 TATACTGTGG CTCTGTGACA ATGGCTGGGC CAATTAGCTG AGGCCCTGTG GTGCAGATTA 181 GGGGTATGGG AGCTGGGGTC AAAGACATAC CCCCTGGTGA CAGAGGGGAG GCTGGAGTCT 241 CCTAGGTCAG TGCTTCTTGA AAACAGGGCT AGTGAGTATT CCTAGGCTCT GGGGCTGAAG 301 CCCTCCCCTC TGCACCAGCC AATGATGGGG CCTGGCCTTG AGCCCCCCAC CCCACCCCCA 361 AGAGAGGGCA GATGGCCAGA AAGCCAGGCT TGGCCCGGCA ATCTGTCGCC TCAAACTGTA 421 GCTCTGGCGC CTGGGCTGTG ACCCCTGCCC TTGGCTGATG ATGAAACCTG TCCTGAGCGG 481 CCACAGTGAT GCCTGACAGT CAGGGAATAC CATCATGTCC CCAAAAGACT GGCTCCCCAG 541 TCCCAGGTCT AGGCCTGCCT GCCTCTCAGT TTCCCCTTGT TGTACTTTGT ACCTTGCCAC 601 AACCTTGCTG GTCTCTTGTG TCTTGAGAAC CCTGTCTTTG GCCTTACCAG TTCCTAAACC 661 CCCCTTTGAG GGAGCATGTG GAACAAATGA GAAGCCCCAG TGAGTAGCCG CCGAGGGGTA 721 GAAGCATTCC CAGAATTGGG AATTGGACAG GGTTGGTTCC GGATGCGCCC TGTGTGACTC 781 TGGCATGCTG TCCAGTAGTC TATCTGTGGA GTAAACGCTT TGTACACCAC GGAGTCCAGG 841 TAATAGAAAC CAAACCACAG CACACAGGCT TTAGCAGTAC CCCAAGAACC AGCTACAAAA 901 GCCCTGGTGT GGCCTGTGTA CCAGTCTCTG GTGTATGGAG TCCAGCACGG CATGGGGAGG 961 TTCTGGCATA ATACCACTGC ACTTGGAGCT ATGTGATAGG CAGTCGCCCT CCTACTAAGC 1021 CAAGTCTCAC ACTCTACTGT GTCACCTGTG CTTAGAGGAG GACAGCATTT GAGACTGGAA 1081 TGAATCATCA AGCCAGACCC CTGGCGGGAT TCAGATGGAA CCTTG //
Primer: Luc_R Sequence file: RDB15720_A7Lpb.seq
>D05137A1_A7Lp_1_Luc-R_B05_05_ABI24.ab1 1 AGAATGTCGG GGCACAGAGC GTAGCGCTTC ATGGCTTTGT GCAGCTGCTC GCCGGCGGTC 61 CCGTCTTCGA GTGGGTAGAA TGGCGCTGGG CCCTTCTTAA TGTTTTTGGC ATCTTCCATG 121 GTGGCTTTAC CAACAGTACC GGATTGCCAA GCTTGGCCGC CGAGGCCAGA TCTTGATATC 181 CTCGAGTGAG CTGGTGTCTA GGAGCCAGTC CCAGTCCTTT TTATGGGACC AGTGGGAGGA 241 GGGTCACCTG AGCCCCCTCC CTTTTGCCCT GACTCATTTC CAAGAAGATA GTGGCGCCAA 301 ACAGGCGGGA GAGATAAGCG GGAACAGAGT GACAGGGGAC AGGGTTATGT CACTCTCAGC 361 CAGCAGAGGT GCATTCAGAA GGTGGGGCAG AGCCATGGAC AACAAGCCTC CAGCCAGCCA 421 CCTGTGATGT CACCCCATGG CTCTCAGAAA AATCCCCCTC TTTTACCCTA AACATTGATT 481 GGTGACTGGA AGTATTGTGC CTCTCCCCCC TTCAGTGTCC TCAGTATGGC TGTCACTCGC 541 TGGGCACCTC AGGATCCAGC AATGGGGAAC ATATATGCAG GGTGCATCCC AGCATGAGTT 601 AGGCACCGTG GCCAAGGGAT TGGACAGATG CCCAAGAGTT TGTGTCAGCC CCAACATGCG 661 CCGTGGTGTC CAACATGAAG ACCGTATGAG GTAGCTGTCA CATCGGAGTC ACCTTCTGCC 721 TATAGTGGCA GCAGTCATTC ATAGCTGGAG CCAAGGCATT TCATAGGCCA GCATATGAAC 781 TAACGCACTG GCTTCACATG CCAGTGAGTG TACTGATTCT GGGTCTTTAA TACCAGTGCC 841 AACTCACTTT TTGTGTTAAC AGCTGGTGCT ACTTGGCCTC TAAGTGGCTG CCTGCTTCCT 901 CCTGGGCTCT ATTAGTGTTT ATGCTTAAAG CTGCCTTCCA GTCACTGACC GCACGGGTCT 961 GGCTTCATGA TCATCCAGTC CCTCAAATGC TGTCTCCCTC TAAGCACAGG TGACACAGTA 1021 GAGTGTGAGA CTTGGCTTAG TAGAGCCGAC TGCCCTATCA CATAGCTTCT AGTTGCCAGT 1081 TGGTAATTAT GCCAGAACCG TTCCCCCAAT TGACG //
Primer: pGCAP_R Sequence file: RDB15720_A7Lpc.seq
>D05137A1_A7Lp_1_pGCAP_R_C05_08_ABI24.ab1 1 AGAAATCTTA TCATGGTCTG CTCGAAGCGG CCGGCCGCCC CGACTCTAGA ATTATTACAC 61 GGCGATCTTG CCGCCCTTCT TGGCCTTAAT GAGAATCTCG CGGATCTTGC GGGCGTCCAA 121 CTTGCCGGTC AGTCCTTTAG GCACCTCGTC CACGAACACA ACACCACCGC GCAGCTTCTT 181 GGCGGTTGTA ACCTGGCTGG CCACATAGTC CACGATCTCC TTCTCGGTCA TGGTTTTACC 241 GTGTTCCAGC ACGACGACTG CGGCGGGCAG CTCGCCGGCA TCGTCGTCGG GCAGGCCGGC 301 GACCCCGGCG TCGAAGATGT TGGGGTGTTG CAGCAGGATG CTCTCCAGTT CGGCTGGGGC 361 TACCTGGTAG CCCTTGTATT TGATCAGGCT CTTCAGCCGG TCCACGATGA AGAAGTGCTC 421 GTCCTCGTCC CAGTAGGCGA TGTCGCCGCT GTGCAGCCAG CCGTCCTTGT CGATGAGAGC 481 GTTTGTAGCC TCGGGGTTGT TAACGTAGCC GCTCATGATC ATGGGGCCAC GGACGCACAG 541 CTCGCCGCGC TGGTTCACAC CCAGTGTCTT ACCGGTGTCC AAGTCCACCA CCTTAGCCTC 601 GAAGAAGGGC ACCACCTTGC CTACTGCGCC AGGCTTGTCG TCCCCTTCGG GGGTGATCAG 661 AATGGCGCTG GTTGTTTCTG TCAGGCCGTA GCCCTGGCGG ATGCCTGGTA GGTGGAAGCG 721 TTTGGCCACG GCCTCACCTA CCTCCTTGCT GAGCGGCGCC CCGCCGCTGG CGATCTCGTG 781 CAAGTTGCTT AGGTCGTACT TGTCGATGAG AGTGCTCTTA GCGAAGAAGC TAAATAGTGT 841 GGGCACCAGC AGGGCAGATT GAATCTTATA GTCTTGCAAG CTGCGCAAGA ATAGCTCCTC 901 CTCGAAGCGG TACATGAGCA CGACCCCGAA AGCCGCAGAT CAAGTAGCCC CAGCGTGGGT 961 GAAACATGCC GAAGCCGTGG GTGAAATGGC ACCACGCTGA GGGATAGCCG GGTGTCGGGG 1021 ATGATCCTGG TTGCCGAAGA TGGGGGTCCG CGGGGCCATG ACTGATCGGA CCACAAGCGG 1081 TGCCGGTGCG GTTAAGGACT ACGACCCCTT TGGGGCCAAT CCTGGGTTAC CTTGGTCA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Kanamori, Y., Interleukin-1β (IL-1β) transcriptionally activates hepcidin by inducing CCAAT enhancer-binding protein δ (C/EBPδ) expression in hepatocytes. J. Biol. Chem. 292 (24): 10275-10287 (2017). PMID 28438835.

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl