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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

rHpcidin(-1861)-luc (#RDB15720)

Reporter plasmid of rat hepcidin antimicrobial peptide (Hamp) promoter fragment (nt -1861 to -35).

Alternative name rhepcidin-luc
Clone info. Reporter plasmid of rat hepcidin antimicrobial peptide (Hamp) promoter fragment (nt -1861 to -35) fused with C-terminal Luciferase, translation initiation site is numbered as +1.
Comment Expression was confirmed by the depositor with luciferase reporter assay.
Vector backbone pGL4 (plasmid)
Size of vector backbone 4.2 kb
Selectable markers Amp^r
Gene/insert name rat Hamp genomic
Depositor|Developer Murakami, Masaru |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kanamori, Y et al. J. Biol. Chem. 292(24):10275-10287, 2017)
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kanamori, Y et al. J. Biol. Chem. 292(24):10275-10287, 2017)。
MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15720 rHpcidin(-1861)-luc DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The rHpcidin(-1861)-luc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15720).

Reference section:

Kanamori, Y., Murakami, M., Sugiyama, M., Hashimoto, O., Matsui, T., Funaba, M., Interleukin-1β (IL-1β) transcriptionally activates hepcidin by inducing CCAAT enhancer-binding protein δ (C/EBPδ) expression in hepatocytes. J. Biol. Chem. 292 (24): 10275-10287 (2017). PMID 28438835. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15720_A7Lpp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F ()
Sequence file: RDB15720_A7Lpa.seq check
>D05137A1_A7Lp_1_pGL4-4174F_A05_02_ABI24.ab1
    1 ATGGGGTCAG ACATTTCTCT GGCCTAACTG GCCGGTACCC CGCCTTGTCT GTCTGTGTGT
   61 CCATGTTGGG CATGGGGTGG GGAAAGAGTC CTAGGGTCCT CTTCAGGTCT AAGCTTAAGT
  121 TATACTGTGG CTCTGTGACA ATGGCTGGGC CAATTAGCTG AGGCCCTGTG GTGCAGATTA
  181 GGGGTATGGG AGCTGGGGTC AAAGACATAC CCCCTGGTGA CAGAGGGGAG GCTGGAGTCT
  241 CCTAGGTCAG TGCTTCTTGA AAACAGGGCT AGTGAGTATT CCTAGGCTCT GGGGCTGAAG
  301 CCCTCCCCTC TGCACCAGCC AATGATGGGG CCTGGCCTTG AGCCCCCCAC CCCACCCCCA
  361 AGAGAGGGCA GATGGCCAGA AAGCCAGGCT TGGCCCGGCA ATCTGTCGCC TCAAACTGTA
  421 GCTCTGGCGC CTGGGCTGTG ACCCCTGCCC TTGGCTGATG ATGAAACCTG TCCTGAGCGG
  481 CCACAGTGAT GCCTGACAGT CAGGGAATAC CATCATGTCC CCAAAAGACT GGCTCCCCAG
  541 TCCCAGGTCT AGGCCTGCCT GCCTCTCAGT TTCCCCTTGT TGTACTTTGT ACCTTGCCAC
  601 AACCTTGCTG GTCTCTTGTG TCTTGAGAAC CCTGTCTTTG GCCTTACCAG TTCCTAAACC
  661 CCCCTTTGAG GGAGCATGTG GAACAAATGA GAAGCCCCAG TGAGTAGCCG CCGAGGGGTA
  721 GAAGCATTCC CAGAATTGGG AATTGGACAG GGTTGGTTCC GGATGCGCCC TGTGTGACTC
  781 TGGCATGCTG TCCAGTAGTC TATCTGTGGA GTAAACGCTT TGTACACCAC GGAGTCCAGG
  841 TAATAGAAAC CAAACCACAG CACACAGGCT TTAGCAGTAC CCCAAGAACC AGCTACAAAA
  901 GCCCTGGTGT GGCCTGTGTA CCAGTCTCTG GTGTATGGAG TCCAGCACGG CATGGGGAGG
  961 TTCTGGCATA ATACCACTGC ACTTGGAGCT ATGTGATAGG CAGTCGCCCT CCTACTAAGC
 1021 CAAGTCTCAC ACTCTACTGT GTCACCTGTG CTTAGAGGAG GACAGCATTT GAGACTGGAA
1081 TGAATCATCA AGCCAGACCC CTGGCGGGAT TCAGATGGAA CCTTG
//
Primer: Luc_R ()
Sequence file: RDB15720_A7Lpb.seq check
>D05137A1_A7Lp_1_Luc-R_B05_05_ABI24.ab1
    1 AGAATGTCGG GGCACAGAGC GTAGCGCTTC ATGGCTTTGT GCAGCTGCTC GCCGGCGGTC
   61 CCGTCTTCGA GTGGGTAGAA TGGCGCTGGG CCCTTCTTAA TGTTTTTGGC ATCTTCCATG
  121 GTGGCTTTAC CAACAGTACC GGATTGCCAA GCTTGGCCGC CGAGGCCAGA TCTTGATATC
  181 CTCGAGTGAG CTGGTGTCTA GGAGCCAGTC CCAGTCCTTT TTATGGGACC AGTGGGAGGA
  241 GGGTCACCTG AGCCCCCTCC CTTTTGCCCT GACTCATTTC CAAGAAGATA GTGGCGCCAA
  301 ACAGGCGGGA GAGATAAGCG GGAACAGAGT GACAGGGGAC AGGGTTATGT CACTCTCAGC
  361 CAGCAGAGGT GCATTCAGAA GGTGGGGCAG AGCCATGGAC AACAAGCCTC CAGCCAGCCA
  421 CCTGTGATGT CACCCCATGG CTCTCAGAAA AATCCCCCTC TTTTACCCTA AACATTGATT
  481 GGTGACTGGA AGTATTGTGC CTCTCCCCCC TTCAGTGTCC TCAGTATGGC TGTCACTCGC
  541 TGGGCACCTC AGGATCCAGC AATGGGGAAC ATATATGCAG GGTGCATCCC AGCATGAGTT
  601 AGGCACCGTG GCCAAGGGAT TGGACAGATG CCCAAGAGTT TGTGTCAGCC CCAACATGCG
  661 CCGTGGTGTC CAACATGAAG ACCGTATGAG GTAGCTGTCA CATCGGAGTC ACCTTCTGCC
  721 TATAGTGGCA GCAGTCATTC ATAGCTGGAG CCAAGGCATT TCATAGGCCA GCATATGAAC
  781 TAACGCACTG GCTTCACATG CCAGTGAGTG TACTGATTCT GGGTCTTTAA TACCAGTGCC
  841 AACTCACTTT TTGTGTTAAC AGCTGGTGCT ACTTGGCCTC TAAGTGGCTG CCTGCTTCCT
  901 CCTGGGCTCT ATTAGTGTTT ATGCTTAAAG CTGCCTTCCA GTCACTGACC GCACGGGTCT
  961 GGCTTCATGA TCATCCAGTC CCTCAAATGC TGTCTCCCTC TAAGCACAGG TGACACAGTA
 1021 GAGTGTGAGA CTTGGCTTAG TAGAGCCGAC TGCCCTATCA CATAGCTTCT AGTTGCCAGT
1081 TGGTAATTAT GCCAGAACCG TTCCCCCAAT TGACG
//
Primer: pGCAP_R ()
Sequence file: RDB15720_A7Lpc.seq check
>D05137A1_A7Lp_1_pGCAP_R_C05_08_ABI24.ab1
    1 AGAAATCTTA TCATGGTCTG CTCGAAGCGG CCGGCCGCCC CGACTCTAGA ATTATTACAC
   61 GGCGATCTTG CCGCCCTTCT TGGCCTTAAT GAGAATCTCG CGGATCTTGC GGGCGTCCAA
  121 CTTGCCGGTC AGTCCTTTAG GCACCTCGTC CACGAACACA ACACCACCGC GCAGCTTCTT
  181 GGCGGTTGTA ACCTGGCTGG CCACATAGTC CACGATCTCC TTCTCGGTCA TGGTTTTACC
  241 GTGTTCCAGC ACGACGACTG CGGCGGGCAG CTCGCCGGCA TCGTCGTCGG GCAGGCCGGC
  301 GACCCCGGCG TCGAAGATGT TGGGGTGTTG CAGCAGGATG CTCTCCAGTT CGGCTGGGGC
  361 TACCTGGTAG CCCTTGTATT TGATCAGGCT CTTCAGCCGG TCCACGATGA AGAAGTGCTC
  421 GTCCTCGTCC CAGTAGGCGA TGTCGCCGCT GTGCAGCCAG CCGTCCTTGT CGATGAGAGC
  481 GTTTGTAGCC TCGGGGTTGT TAACGTAGCC GCTCATGATC ATGGGGCCAC GGACGCACAG
  541 CTCGCCGCGC TGGTTCACAC CCAGTGTCTT ACCGGTGTCC AAGTCCACCA CCTTAGCCTC
  601 GAAGAAGGGC ACCACCTTGC CTACTGCGCC AGGCTTGTCG TCCCCTTCGG GGGTGATCAG
  661 AATGGCGCTG GTTGTTTCTG TCAGGCCGTA GCCCTGGCGG ATGCCTGGTA GGTGGAAGCG
  721 TTTGGCCACG GCCTCACCTA CCTCCTTGCT GAGCGGCGCC CCGCCGCTGG CGATCTCGTG
  781 CAAGTTGCTT AGGTCGTACT TGTCGATGAG AGTGCTCTTA GCGAAGAAGC TAAATAGTGT
  841 GGGCACCAGC AGGGCAGATT GAATCTTATA GTCTTGCAAG CTGCGCAAGA ATAGCTCCTC
  901 CTCGAAGCGG TACATGAGCA CGACCCCGAA AGCCGCAGAT CAAGTAGCCC CAGCGTGGGT
  961 GAAACATGCC GAAGCCGTGG GTGAAATGGC ACCACGCTGA GGGATAGCCG GGTGTCGGGG
 1021 ATGATCCTGG TTGCCGAAGA TGGGGGTCCG CGGGGCCATG ACTGATCGGA CCACAAGCGG
1081 TGCCGGTGCG GTTAAGGACT ACGACCCCTT TGGGGCCAAT CCTGGGTTAC CTTGGTCA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kanamori, Y., Interleukin-1β (IL-1β) transcriptionally activates hepcidin by inducing CCAAT enhancer-binding protein δ (C/EBPδ) expression in hepatocytes. J. Biol. Chem. 292 (24): 10275-10287 (2017). PMID 28438835. [link to RRC of NBRP]