Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pSPB1 (#RDB15715)

Expression vector of Streptococcus pneumoniae G54 peptidase domain (PEP) of BlpA.

Clone info. Expression vector of Streptococcus pneumoniae G54 peptidase domain (PEP) of BlpA, tagged with His at C-termonus.
Comment Correspinding nucleotide 475628 to 476077 of GenBank CP001015 was chemically synthesized.
Vector backbone pET21-b (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r
Gene/insert name Streptococcus pneumoniae G54 blpA genomic
Depositor|Developer Ishii, Seiji |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishii, S et al. Sci. Rep. 7(1): 4029, 2017)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishii, S et al. Sci. Rep. 7(1): 4029, 2017)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15715 pSPB1 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pSPB1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15715).

Reference section:

Ishii, S., Fukui, K., Yokoshima, S., Kumagai, K., Beniyama, Y., Kodama, T., Fukuyama, T., Okabe, T., Nagano, T., Kojima, H., Yano, T., High-throughput Screening of Small Molecule Inhibitors of the Streptococcus Quorum-sensing Signal Pathway. Sci. Rep. 7 (1): 4029 (2017). PMID 28642545. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15716_A7Lcp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pBR_BamHI_R2
Sequence file: RDB15716_A7Lca.seq check
1
Primer: lacpro_F
Sequence file: RDB15716_A7Lcb.seq check
1

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ishii, S., High-throughput Screening of Small Molecule Inhibitors of the Streptococcus Quorum-sensing Signal Pathway. Sci. Rep. 7 (1): 4029 (2017). PMID 28642545. [link to RRC of NBRP]