Expression vector of Streptococcus pneumoniae G54 peptidase domain (PEP) of BlpA.
Clone info. | Expression vector of Streptococcus pneumoniae G54 peptidase domain (PEP) of BlpA, tagged with His at C-termonus. |
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Comment | Correspinding nucleotide 475628 to 476077 of GenBank CP001015 was chemically synthesized. |
Vector backbone | pET21-b (plasmid) |
Size of vector backbone | 5.4 kb |
Selectable markers | Amp^r |
Gene/insert name | Streptococcus pneumoniae G54 blpA genomic |
Depositor|Developer | Ishii, Seiji | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishii, S et al. Sci. Rep. 7(1): 4029, 2017) |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishii, S et al. Sci. Rep. 7(1): 4029, 2017)。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB15715 | pSPB1 | DNA solution |
Materials & Methods section:
The pSPB1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15715). |
Reference section:
Ishii, S., Fukui, K., Yokoshima, S., Kumagai, K., Beniyama, Y., Kodama, T., Fukuyama, T., Okabe, T., Nagano, T., Kojima, H., Yano, T., High-throughput Screening of Small Molecule Inhibitors of the Streptococcus Quorum-sensing Signal Pathway. Sci. Rep. 7 (1): 4029 (2017). PMID 28642545. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB15716_A7Lcp1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: pBR_BamHI_R2 Sequence file: RDB15716_A7Lca.seq ![]() |
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Primer: lacpro_F Sequence file: RDB15716_A7Lcb.seq ![]() |
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Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Ishii, S., High-throughput Screening of Small Molecule Inhibitors of the Streptococcus Quorum-sensing Signal Pathway. Sci. Rep. 7 (1): 4029 (2017). PMID 28642545. [link to RRC of NBRP] |
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