Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pSPB1

Expression vector of Streptococcus pneumoniae G54 peptidase domain (PEP) of BlpA.

Catalog number RDB15715
Resource name pSPB1
Clone info. Expression vector of Streptococcus pneumoniae G54 peptidase domain (PEP) of BlpA, tagged with His at C-termonus.
Correspinding nucleotide 475628 to 476077 of GenBank CP001015 was chemically synthesized.
Vector backbone pET21-b (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r
Gene/insert name Streptococcus pneumoniae G54 blpA genomic
Depositor|Developer Ishii, Seiji |
 
Other clones in our bank Streptococcus pneumoniae G54 (KEGG orthology K20344) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishii, S et al. Sci. Rep. 7(1): 4029, 2017)
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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RDB15715 pSPB1 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Ishii, S., High-throughput Screening of Small Molecule Inhibitors of the Streptococcus Quorum-sensing Signal Pathway. Sci. Rep. 7 (1): 4029 (2017). PMID 28642545.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15716_A7Lcp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pBR_BamHI_R2
Sequence file: RDB15716_A7Lca.seq check
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Primer: lacpro_F
Sequence file: RDB15716_A7Lcb.seq check
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Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Ishii, S., High-throughput Screening of Small Molecule Inhibitors of the Streptococcus Quorum-sensing Signal Pathway. Sci. Rep. 7 (1): 4029 (2017). PMID 28642545.

2021.03.25

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