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pStMuPEP1(WT)

Expression vector of Streptococcus mutans peptidase domain 1 (PEP1) of ComA, wild type.

Catalog number RDB15713
Resource name pStMuPEP1(WT)
Clone info. Expression vector of Streptococcus mutans peptidase domain 1 (PEP1) of ComA, wild type. The hydrophobic N-terminal regions (48 amino acids, comA) has been truncated from the PEP, tagged with His at C-terminus.
PEP of ComA PEP of S. mutans (strain ATCC No. 700610) was generated by PCR from pSMuP1. The primers used were 5'-ACATATGTATAAGCTAGTACCTCAGATTGATAC-3' (forward) and 5'-AGCTTATACATATGTATATCTCCTTCTTAAAGT-3' (reverse). Reference of genome sequence, GenBank AE014133.
Vector backbone pET21-b (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r
Gene/insert name Streptococcus mutans comA genomic
Depositor|Developer Ishii, Seiji |
 
Other clones in our bank Streptococcus mutans (KEGG orthology K20344) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishii, S et al. Sci. Rep. 7(1): 4029, 2017)
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RDB15713 pStMuPEP1(WT) DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Ishii, S., High-throughput Screening of Small Molecule Inhibitors of the Streptococcus Quorum-sensing Signal Pathway. Sci. Rep. 7 (1): 4029 (2017). PMID 28642545.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15713_A7Lcp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pBR_BamHI_R2
Sequence file: RDB15713_A7Lca.seq check
>D05129A1_A7Lc_1_pBR_BamHI_R2_A11_02_ABI24.ab1
    1 CTTGCTGCCA GAGTTTCGAG CGTAGCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC
   61 TCGATCCCGC GAAATTAATA CGACTCACTA TAGGGGAATT GTGAGCGGAT AACAATTCCC
  121 CTCTAGAAAT AATTTTGTTT AACTTTAAGA AGGAGATATA CATATGTATA AGCTAGTACC
  181 TCAGATTGAT ACCAGAGACT GTGGGCCGGC AGTGCTGGCA TCTGTTGCAA AGCATTACGG
  241 ATCTAATTAC TCTATCGCTT ATCTGCGGGA ACTCTCAAAG ACTAACAAGC AGGGAACAAC
  301 AGCTCTTGGC ATTGTTGAAG CTGCTAAAAA GTTAGGCTTT GAAACACGCT CTATCAAGGC
  361 GGATATGACG CTTTTTGATT ATAATGATTT GACCTATCCT TTTATCGTCC ATGTGATTAA
  421 AGGAAAACGT CTGCAGCATT ATTATGTCGT CTATGGCAGC CAGAATAATC AGCTGATTAT
  481 TGGAGATCCT GATCCTTCAG TTAAGGTGAC TAGGATGAGT AAGGAACGCT TTCAATCAGA
  541 GTGGACAGGC CTTGCAATTT TCCTAGCTCC TCAGCCTAAC TATAAGCCTC ATAAAGGTGA
  601 ACACCACCAC CACCACCACT AGGTCGACAA GCTTGCGGCC GCACTCGAGC ACCACCACCA
  661 CCACCACTGA GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC
  721 CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT
  781 GCTGAAAGGA GGAACTATAT CCGGATTGGC GAATGGGACG CGCCCTGTAG CGGCGCATTA
  841 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG
  901 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA
  961 GCTCTAAATC GGGGGGCTCC CTTTAGGGTT CCGGATTTAG TGCTTTACGG CACCTCGACC
 1021 CCAAAAACTT TGATTAGGGT GATGGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGATT
 1081 TTTCGCCCTT TGACGTTGAA GTTCACGATT CTTTAATAGT GACTTCTTGT TCCAAACCTG
1141 GAAACCAAAC AGCT
//
Primer: lacpro_F
Sequence file: RDB15713_A7Lcb.seq check
>D05129A1_A7Lc_1_laclpro_F_C11_08_ABI24.ab1
    1 GATATTAGGT ACATTATTTC GGATGCGCCG GAGAGAGTCA ATTCAGGGTG GTGAATGTGA
   61 AACCAGTAAC GTTATACGAT GTCGCAGAGT ATGCCGGTGT CTCTTATCAG ACCGTTTCCC
  121 GCGTGGTGAA CCAGGCCAGC CACGTTTCTG CGAAAACGCG GGAAAAAGTG GAAGCGGCGA
  181 TGGCGGAGCT GAATTACATT CCCAACCGCG TGGCACAACA ACTGGCGGGC AAACAGTCGT
  241 TGCTGATTGG CGTTGCCACC TCCAGTCTGG CCCTGCACGC GCCGTCGCAA ATTGTCGCGG
  301 CGATTAAATC TCGCGCCGAT CAACTGGGTG CCAGCGTGGT GGTGTCGATG GTAGAACGAA
  361 GCGGCGTCGA AGCCTGTAAA GCGGCGGTGC ACAATCTTCT CGCGCAACGC GTCAGTGGGC
  421 TGATCATTAA CTATCCGCTG GATGACCAGG ATGCCATTGC TGTGGAAGCT GCCTGCACTA
  481 ATGTTCCGGC GTTATTTCTT GATGTCTCTG ACCAGACACC CATCAACAGT ATTATTTTCT
  541 CCCATGAAGA CGGTACGCGA CTGGGCGTGG AGCATCTGGT CGCATTGGGT CACCAGCAAA
  601 TCGCGCTGTT AGCGGGCCCA TTAAGTTCTG TCTCGGCGCG TCTGCGTCTG GCTGGCTGGC
  661 ATAAATATCT CACTCGCAAT CAAATTCAGC CGATAGCGGA ACGGGAAGGC GACTGGAGTG
  721 CCATGTCCGG TTTTCAACAA ACCATGCAAA TGCTGAATGA GGGCATCGTT CCCACTGCGA
  781 TGCTGGTTGC CAACGATCAG ATGGCGCTGG GCGCAATGCG CGCCATTACC GAGTCCGGGC
  841 TGCGCGTTGG TGCGGATATC TCGGTAGTGG GATACGACGA TACCGAAGAC AGCTCATGTT
  901 ATATCCCGCC GTTAACCACC ATCAAACAGG ATTTTCGCCT GCTGGGGCAA ACCAGCGTGG
  961 ACCGCTTGCT GCAACTCTCT CAGGGCCCAG GCGGTGAAAG GGCAATCAGC TGTTGCCCGT
 1021 CTCACTGGTG AAAAGAAAAA ACCACCCTGG CGCCCAATAC GCAAACCCGC TCTCCCCGCG
 1081 CGTTGGCCCG ATTTCATATG CCAGCCTGGG CCACGACAGG TTCCCGACTG GAAAAGCCGG
1141 GGCCAATGGG AGC
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Ishii, S., High-throughput Screening of Small Molecule Inhibitors of the Streptococcus Quorum-sensing Signal Pathway. Sci. Rep. 7 (1): 4029 (2017). PMID 28642545.

2021.03.25

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