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pENTER-EGFR T790M/L858R

Gateway(R) entry vector of human epidermal growth factor receptor (EGFR), EGFR-tyrosine kinase inhibitor (TKI) resistant and activating mutations.

Catalog number RDB15494
Resource name pENTER-EGFR T790M/L858R
Clone info. Gateway(R) entry vector of human epidermal growth factor receptor (EGFR) mutant, T790M and L858R, EGFR-tyrosine kinase inhibitor (TKI) resistant and activating mutations.
cDNA was obtained from A549 cells. Expression was confirmed by the depositor with western blotting after cloned into pLenti6.3 vector (by LR clonase II reaction).
Vector backbone pENTR (plasmid)
Size of vector backbone 2.6 kb
Selectable markers Kan^r
Gene/insert name human EGFR cDNA
Depositor Katayama, Ryohei |
 
Other clones in our bank human EGFR (NCBI Gene 1956) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Uchibori, K et al. Nat. Commun. 8: 14768, 2017)
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Remarks
Gateway® Entry clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
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RDB15494 pENTER-EGFR T790M/L858R DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Uchibori, K., Brigatinib combined with anti-EGFR antibody overcomes osimertinib resistance in EGFR-mutated non-small-cell lung cancer. Nat. Commun. 8: 14768 (2017). PMID 28287083.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15494_A7K8p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Region: rrnB T1 ter, attL1, insert 5' | Primer: rrnBT1ter_F2 | Sequence: RDB15494_A7K8a.seq
>D04925A7_A7K8_2_rrnBT1ter_F2_G04_19_ABI24.ab1
    1 AGCCAGTCTT CGACTGAGCC TTTCGTTTTA TTTGATGCCT GGCAGTTCCC TACTCTCGCG
   61 TTAACGCTAG CATGGATGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTCTTAAG
  121 CTCGGGCCCC AAATAATGAT TTTATTTTGA CTGATAGTGA CCTGTTCGTT GCAACAAATT
  181 GATGAGCAAT GCTTTTTTAT AATGCCAACT TTGTACAAAA AAGCAGGCTC CGCGGCCGCC
  241 CCCTTCACCA TGCGACCCTC CGGGACGGCC GGGGCAGCGC TCCTGGCGCT GCTGGCTGCG
  301 CTCTGCCCGG CGAGTCGGGC TCTGGAGGAA AAGAAAGTTT GCCAAGGCAC GAGTAACAAG
  361 CTCACGCAGT TGGGCACTTT TGAAGATCAT TTTCTCAGCC TCCAGAGGAT GTTCAATAAC
  421 TGTGAGGTGG TCCTTGGGAA TTTGGAAATT ACCTATGTGC AGAGGAATTA TGATCTTTCC
  481 TTCTTAAAGA CCATCCAGGA GGTGGCTGGT TATGTCCTCA TTGCCCTCAA CACAGTGGAG
  541 CGAATTCCTT TGGAAAACCT GCAGATCATC AGAGGAAATA TGTACTACGA AAATTCCTAT
  601 GCCTTAGCAG TCTTATCTAA CTATGATGCA AATAAAACCG GACTGAAGGA GCTGCCCATG
  661 AGAAATTTAC AGGAAATCCT GCATGGCGCC GTGCGGTTCA GCAACAACCC TGCCCTGTGC
  721 AATGTGGAGA GCATCCAGTG GCGGGACATA GTCAGCAGTG ACTTTCTCAG CAACATGTCG
  781 ATGGACTTCC AGAACCACCT GGGCAGCTGC CAAAAGTGTG ATCCAAGCTG TCCCAATGGG
  841 AGCTGCTGGG GTGCAGGAGA GGAGAACTGC CAGAAACTGA CCAAAATCAT CTGTGCCCAG
  901 CAGTGCTCCG GGCGCTGCCG TGGCAAGTCC CCCCAGTGAC TGCTGCCACA ACCAGTGTGC
  961 TGCAGGCTGC ACAGGGCCCC CGGGAGAGCG ACTGCCCTGG TCTGCCGCAA ATTCCGAGAC
 1021 GAAGCCACGT GCAAAGGACA CCTGCCCCCC CCACTCATGC TCTACAAACC CCCACCACGT
1081 TACAGATTGG ATGGTGGAAA TCCCGAGGGA CAAAATAACA AGCCTTTTGA T
//
Region: attL2, insert 3' | Primer: pDONR_R | Sequence: RDB15494_A7K8b.seq
>D04925A7_A7K8_2_pDONR_R_G05_20_ABI24.ab1
    1 GCGGACGCAG GACGCTATGA CCATGTAATA CGACTCACTA TAGGGGATAT CAGCTGGATG
   61 GCAAATAATG ATTTTATTTT GACTGATAGT GACCTGTTCG TTGCAACAAA TTGATAAGCA
  121 ATGCTTTCTT ATAATGCCAA CTTTGTACAA GAAAGCTGGG TCGGCGCGCC CACCCTTTCA
  181 TGCTCCAATA AATTCACTGC TTTGTGGCGC GACCCTTAGG TATTCTGCAT TTTCAGCTGT
  241 GGAGCCCTTA AAGATGCCAT TTGGCTTGGC TTCCTTGGGA AAGAAGTCCT GCTGGTAGTC
  301 AGGGTTGTCC AGGCTAATTT GGTGGCTGCC TTTCTGGGCC CAGTGGGCAG GGCTGTCGAA
  361 TGTGCTGTTG ACACAGGTGG GCTGGACAGT GTTGAGATAC TCGGGGTTGC CCACTGCAGT
  421 GCTGTGGGGG TCCTGGTAGT GTGGGTCTCT GCTGGGCGCG GGGTTCAGAG GCTGATTGTG
  481 ATAGACAGGA TTCTGCACAG AGCCAGCGGG CCTTTTGGGA ACGGACTGGT TTATGTATTC
  541 AGGCACTGGG AGGAAGGTGT CGTCTATGCT GTCCTCAGTC AAGGCGCCTG TGGGGTCTGA
  601 GCTGTATCGC TGCAAGAAGC TGTCTTCCTT GATGGGACAG CTTTGCAGCC CATTTCTATC
  661 AATGCAAGCC ACGGTGGAAT TGTTGCTGGT TGCACTCAGA GAGCTCAGGA GGGGAGTCCG
  721 TGACGTGGAG GGGCTGCTGA AGAAGCCCTG CTGTGGGATG AGGTACTCGT CGGCATCCAC
  781 CACGTCGTCC ATGTCTTCTT CATCCATCAG GGCACGGTAG AAGTTGGAGT CTGTAGGACT
  841 TGGCAAATGC ATTCTTTCAT CCCCCTGAAT GACAAGGTAG CGCTGGGGGT CTCGGGCCAT
  901 TTTGGAGAAT TCGATGATCA ACTCACGGAA CTTTGGGCGA CTATCTGCGT CTATCATCCA
  961 GCACTTGACC ATGATCATGT AGACATCGAT GGTACATATG GGTGGCTGAG GAGGCGTTCT
 1021 CCTTTCTCCA GGATGGAGGA GATCTCGCTG GCAGGATTCC CGTCATATGG ACATGAATTC
1081 GAAAGGTCCA TCCAACCTTC TCAAACCG
//
Region: insert middle region (T790M, L858R) | Primer: EGFR-F5 | Sequence: RDB15494_A7K8c.seq
>D04925A7_A7K8_2_EGFR-F5_G06_21_ABI24.ab1
    1 GAAATAATCG TCATCGCCAC TGGGATGGTG GGGGCCCTCC TCTTGCTGCT GGTGGTGGCC
   61 CTGGGGATCG GCCTCTTCAT GCGAAGGCGC CACATCGTTC GGAAGCGCAC GCTGCGGAGG
  121 CTGCTGCAGG AGAGGGAGCT TGTGGAGCCT CTTACACCCA GTGGAGAAGC TCCCAACCAA
  181 GCTCTCTTGA GGATCTTGAA GGAAACTGAA TTCAAAAAGA TCAAAGTGCT GGGCTCCGGT
  241 GCGTTCGGCA CGGTGTATAA GGGACTCTGG ATCCCAGAAG GTGAGAAAGT TAAAATTCCC
  301 GTCGCTATCA AGGAATTAAG AGAAGCAACA TCTCCGAAAG CCAACAAGGA AATCCTCGAT
  361 GAAGCCTACG TGATGGCCAG CGTGGACAAC CCCCACGTGT GCCGCCTGCT GGGCATCTGC
  421 CTCACCTCCA CCGTGCAGCT CATCATGCAG CTCATGCCCT TCGGCTGCCT CCTGGACTAT
  481 GTCCGGGAAC ACAAAGACAA TATTGGCTCC CAGTACCTGC TCAACTGGTG TGTGCAGATC
  541 GCAAAGGGCA TGAACTACTT GGAGGACCGT CGCTTGGTGC ACCGCGACCT GGCAGCCAGG
  601 AACGTACTGG TGAAAACACC GCAGCATGTC AAGATCACAG ATTTTGGGCG GGCCAAACTG
  661 CTGGGTGCGG AAGAGAAAGA ATACCATGCA GAAGGAGGCA AAGTGCCTAT CAAGTGGATG
  721 GCATTGGAAT CAATTTTACA CAGAATCTAT ACCCACCAGA GTGATGTCTG GAGCTACGGG
  781 GTGACCGTTT GGGAGTTGAT GACCTTTGGA TCCAAGCCAT ATGACGGAAT CCCTGCCAGC
  841 GAGATCTCCT CCATCCTGGA GAAAGGAGAA CGCCTCCCTC AGCCACCCAT ATGTACCATC
  901 GATGTCTACA TGATCATGGT CAAGTGCTGG ATGATAGACG CAGATAGTCG CCCAAAGTTC
  961 CGTGAGTTTG ATCATCGAAA TTCTCCCAAA ATGGGCCCGA GACCCCCAGC GCTACCCTTT
 1021 GTCATTTCAT GGGGATGAAA GACTGCATTT GGCTAGTCTA CAGACTCCAA CTCTACGTTG
1081 CCTTGATGAA TGAAGGAGAA CATGG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Uchibori, K., Brigatinib combined with anti-EGFR antibody overcomes osimertinib resistance in EGFR-mutated non-small-cell lung cancer. Nat. Commun. 8: 14768 (2017). PMID 28287083.

2019.09.20

GNP_filter3_RDBDEP_html_190816.pl