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pENTER-EGFR L858R

Gateway(R) entry vector of human epidermal growth factor receptor (EGFR), EGFR-activating mutation.

Catalog number RDB15493
Resource name pENTER-EGFR L858R
Clone info. Gateway(R) entry vector of human epidermal growth factor receptor (EGFR) mutant, L858R in exon 21, EGFR-activating mutation.
cDNA was obtained from A549 cells. Expression was confirmed by the depositor with western blotting after cloned into pLenti6.3 vector (by LR clonase II reaction).
Vector backbone pENTR (plasmid)
Size of vector backbone 2.6 kb
Selectable markers Kan^r
Gene/insert name human EGFR cDNA
Depositor Katayama, Ryohei |
 
Other clones in our bank human EGFR (NCBI Gene 1956) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Uchibori, K et al. Nat. Commun. 8: 14768, 2017)
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Remarks
Gateway® Entry clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
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RDB15493 pENTER-EGFR L858R DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Uchibori, K., Brigatinib combined with anti-EGFR antibody overcomes osimertinib resistance in EGFR-mutated non-small-cell lung cancer. Nat. Commun. 8: 14768 (2017). PMID 28287083.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15493_A7K8p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Region: rrnB T1 ter, attL1, insert 5' | Primer: rrnBT1ter_F2 | Sequence: RDB15493_A7K8a.seq
>D04925A6_A7K8_2_rrnBT1ter_F2_F04_16_ABI24.ab1
    1 AAACCAGATT CTTCGACTGA GCCTTTCGTT TTATTTGATG CCTGGCAGTT CCCTACTCTC
   61 GCGTTAACGC TAGCATGGAT GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTCTT
  121 AAGCTCGGGC CCCAAATAAT GATTTTATTT TGACTGATAG TGACCTGTTC GTTGCAACAA
  181 ATTGATGAGC AATGCTTTTT TATAATGCCA ACTTTGTACA AAAAAGCAGG CTCCGCGGCC
  241 GCCCCCTTCA CCATGCGACC CTCCGGGACG GCCGGGGCAG CGCTCCTGGC GCTGCTGGCT
  301 GCGCTCTGCC CGGCGAGTCG GGCTCTGGAG GAAAAGAAAG TTTGCCAAGG CACGAGTAAC
  361 AAGCTCACGC AGTTGGGCAC TTTTGAAGAT CATTTTCTCA GCCTCCAGAG GATGTTCAAT
  421 AACTGTGAGG TGGTCCTTGG GAATTTGGAA ATTACCTATG TGCAGAGGAA TTATGATCTT
  481 TCCTTCTTAA AGACCATCCA GGAGGTGGCT GGTTATGTCC TCATTGCCCT CAACACAGTG
  541 GAGCGAATTC CTTTGGAAAA CCTGCAGATC ATCAGAGGAA ATATGTACTA CGAAAATTCC
  601 TATGCCTTAG CAGTCTTATC TAACTATGAT GCAAATAAAA CCGGACTGAA GGAGCTGCCC
  661 ATGAGAAATT TACAGGAAAT CCTGCATGGC GCCGTGCGGT TCAGCAACAA CCCTGCCCTG
  721 TGCAATGTGG AGAGCATCCA GTGGCGGGAC ATAGTCAGCA GTGACTTTCT CAGCAACATG
  781 TCGATGGACT TCCAGAACCA CCTGGGCAGC TGCCAAAAGT GTGATCCAAG CTGTCCCAAT
  841 GGGAGCTGCT GGGGTGCAGG AGAGGAGAAC TGCCAGAAAC TGACCAAAAT CATCTGTGCC
  901 CAGCAGTGCT CCGGGCGCTG CCGTGGCAAG TCCCCCCAGT GACTGCTGCC ACAACCAGTG
  961 TGCTGCAGCT GCACAGGCCC CCGGGAGAGC GACTGCCTGG TTCTGCCGCA AATTCCGAGA
 1021 CGAAAGCCAC GTGCAAGGAC ACCTGCCCCC CCCACTTCAT GCTCTACACC CCCACCACGT
1081 TACAGATGAT GTGATCTCCG AAGGGCAAAT AACGAGGCTT TTG
//
Region: attL2, insert 3' | Primer: pDONR_R | Sequence: RDB15493_A7K8b.seq
>D04925A6_A7K8_2_pDONR_R_H05_23_ABI24.ab1
    1 GGGGACGTCA GGTACGCTAT GACCATGTAA TACGACTCAC TATAGGGGAT ATCAGCTGGA
   61 TGGCAAATAA TGATTTTATT TTGACTGATA GTGACCTGTT CGTTGCAACA AATTGATAAG
  121 CAATGCTTTC TTATAATGCC AACTTTGTAC AAGAAAGCTG GGTCGGCGCG CCCACCCTTT
  181 CATGCTCCAA TAAATTCACT GCTTTGTGGC GCGACCCTTA GGTATTCTGC ATTTTCAGCT
  241 GTGGAGCCCT TAAAGATGCC ATTTGGCTTG GCTTCCTTGG GAAAGAAGTC CTGCTGGTAG
  301 TCAGGGTTGT CCAGGCTAAT TTGGTGGCTG CCTTTCTGGG CCCAGTGGGC AGGGCTGTCG
  361 AATGTGCTGT TGACACAGGT GGGCTGGACA GTGTTGAGAT ACTCGGGGTT GCCCACTGCA
  421 GTGCTGTGGG GGTCCTGGTA GTGTGGGTCT CTGCTGGGCG CGGGGTTCAG AGGCTGATTG
  481 TGATAGACAG GATTCTGCAC AGAGCCAGCG GGCCTTTTGG GAACGGACTG GTTTATGTAT
  541 TCAGGCACTG GGAGGAAGGT GTCGTCTATG CTGTCCTCAG TCAAGGCGCC TGTGGGGTCT
  601 GAGCTGTATC GCTGCAAGAA GCTGTCTTCC TTGATGGGAC AGCTTTGCAG CCCATTTCTA
  661 TCAATGCAAG CCACGGTGGA ATTGTTGCTG GTTGCACTCA GAGAGCTCAG GAGGGGAGTC
  721 CGTGACGTGG AGGGGCTGCT GAAGAAGCCC TGCTGTGGGA TGAGGTACTC GTCGGCATCC
  781 ACCACGTCGT CCATGTCTTC TTCATCCATC AGGGCACGGT AGAAGTTGGA GTCTGTAGGA
  841 CTTGGCAAAT GCATTCTTTC ATCCCCCCTG AATGACAAGG TAGCGCTGGG GGGTCTCGGG
  901 CCATTTTTGG AGAAATTCGA TGATCAACTC ACGGGAACTT TGGGGCGACT ATCTGCGTCT
  961 ATCATCCAGC ACTTGACCCA TGATCATGTA GACATCGATG GTACATATGG GTGGGCTGAG
 1021 GGAGGCGTTC TCCTTTCTCC AGGATGGAGG GAGATCTCGC TGGCAGGATT TCCGTCATAT
1081 GCTGAATTCA AAGGTCATCA ACCTTCCCAA AACG
//
Region: insert middle region (L858R) | Primer: EGFR-F5 | Sequence: RDB15493_A7K8c.seq
>D04925A6_A7K8_2_EGFR-F5_F09_18_ABI24.ab1
    1 AACGTAATCG TTCATCGCCA CTGGGATGGT GGGGGCCCTC CTCTTGCTGC TGGTGGTGGC
   61 CCTGGGGATC GGCCTCTTCA TGCGAAGGCG CCACATCGTT CGGAAGCGCA CGCTGCGGAG
  121 GCTGCTGCAG GAGAGGGAGC TTGTGGAGCC TCTTACACCC AGTGGAGAAG CTCCCAACCA
  181 AGCTCTCTTG AGGATCTTGA AGGAAACTGA ATTCAAAAAG ATCAAAGTGC TGGGCTCCGG
  241 TGCGTTCGGC ACGGTGTATA AGGGACTCTG GATCCCAGAA GGTGAGAAAG TTAAAATTCC
  301 CGTCGCTATC AAGGAATTAA GAGAAGCAAC ATCTCCGAAA GCCAACAAGG AAATCCTCGA
  361 TGAAGCCTAC GTGATGGCCA GCGTGGACAA CCCCCACGTG TGCCGCCTGC TGGGCATCTG
  421 CCTCACCTCC ACCGTGCAAC TCATCACGCA GCTCATGCCC TTCGGCTGCC TCCTGGACTA
  481 TGTCCGGGAA CACAAAGACA ATATTGGCTC CCAGTACCTG CTCAACTGGT GTGTGCAGAT
  541 CGCAAAGGGC ATGAACTACT TGGAGGACCG TCGCTTGGTG CACCGCGACC TGGCAGCCAG
  601 GAACGTACTG GTGAAAACAC CGCAGCATGT CAAGATCACA GATTTTGGGC GGGCCAAACT
  661 GCTGGGTGCG GAAGAGAAAG AATACCATGC AGAAGGAGGC AAAGTGCCTA TCAAGTGGAT
  721 GGCATTGGAA TCAATTTTAC ACAGAATCTA TACCCACCAG AGTGATGTCT GGAGCTACGG
  781 GGTGACCGTT TGGGAGTTGA TGACCTTTGG ATCCAAGCCA TATGACGGAA TCCCTGCCAG
  841 CGAGATCTCC TCCATCCTGG AGAAAGGAGA ACGCCTCCCT CAGCCACCCA TATGTACCAT
  901 CGATGTCTAC ATGATCATGG TCAAGTGCTG GATGATAGAC GCAGATAGTC GCCCAAAGTT
  961 TCCGTGAGTT GATCATCGAA TTTCTCCCAA AATGGGCCCG AGACCCCCAG CGCTACCCTT
 1021 TGTTCATTCA TGGGGGAATG AAAGAATGCC ATTTGGCTAG GTTCGTACAG ACCTCCGACT
1081 CTACCGCTGC CGTGGAATGC AATGCAGGAA GGAACCATG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Uchibori, K., Brigatinib combined with anti-EGFR antibody overcomes osimertinib resistance in EGFR-mutated non-small-cell lung cancer. Nat. Commun. 8: 14768 (2017). PMID 28287083.

2019.09.20

GNP_filter3_RDBDEP_html_190816.pl