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pENTER-EGFR C797S/del19

Gateway(R) entry vector of human epidermal growth factor receptor (EGFR), EGFR-tyrosine kinase inhibitor (TKI) resistant and activating mutations.

Catalog number RDB15491
Resource name pENTER-EGFR C797S/del19
Clone info. Gateway(R) entry vector of human epidermal growth factor receptor (EGFR) mutant, C797S and deletion in exon 19, EGFR-tyrosine kinase inhibitor (TKI) resistant and activating mutations.
cDNA was obtained from HCC-827 cells. Expression was confirmed by the depositor with western blotting after cloned into pLenti6.3 vector (by LR clonase II reaction).
Vector backbone pENTR (plasmid)
Size of vector backbone 2.6 kb
Selectable markers Kan^r
Gene/insert name human EGFR cDNA
Depositor Katayama, Ryohei |
 
Other clones in our bank human EGFR (NCBI Gene 1956) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Uchibori, K et al. Nat. Commun. 8: 14768, 2017)
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Remarks
Gateway® Entry clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
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RDB15491 pENTER-EGFR C797S/del19 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Uchibori, K., Brigatinib combined with anti-EGFR antibody overcomes osimertinib resistance in EGFR-mutated non-small-cell lung cancer. Nat. Commun. 8: 14768 (2017). PMID 28287083.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15491_A7K8p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Region: rrnB T1 ter, attL1, insert 5' | Primer: rrnBT1ter_F2 | Sequence: RDB15491_A7K8a.seq
>D04925A4_A7K8_2_rrnBT1ter_F2_D04_10_ABI24.ab1
    1 AGACAGGTCT TCCGACTGAG CCTTTCGTTT TATTTGATGC CTGGCAGTTC CCTACTCTCG
   61 CGTTAACGCT AGCATGGATG TTTTCCCAGT CACGACGTTG TAAAACGACG GCCAGTCTTA
  121 AGCTCGGGCC CCAAATAATG ATTTTATTTT GACTGATAGT GACCTGTTCG TTGCAACAAA
  181 TTGATGAGCA ATGCTTTTTT ATAATGCCAA CTTTGTACAA AAAAGCAGGC TCCGCGGCCG
  241 CCCCCTTCAC CATGCGACCC TCCGGGACGG CCGGGGCAGC GCTCCTGGCG CTGCTGGCTG
  301 CGCTCTGCCC GGCGAGTCGG GCTCTGGAGG AAAAGAAAGT TTGCCAAGGC ACGAGTAACA
  361 AGCTCACGCA GTTGGGCACT TTTGAAGATC ATTTTCTCAG CCTCCAGAGG ATGTTCAATA
  421 ACTGTGAGGT GGTCCTTGGG AATTTGGAAA TTACCTATGT GCAGAGGAAT TATGATCTTT
  481 CCTTCTTAAA GACCATCCAG GAGGTGGCTG GTTATGTCCT CATTGCCCTC AACACAGTGG
  541 AGCGAATTCC TTTGGAAAAC CTGCAGATCA TCAGAGGAAA TATGTACTAC GAAAATTCCT
  601 ATGCCTTAGC AGTCTTATCT AACTATGATG CAAATAAAAC CGGACTGAAG GAGCTGCCCA
  661 TGAGAAATTT ACAGGAAATC CTGCATGGCG CCGTGCGGTT CAGCAACAAC CCTGCCCTGT
  721 GCAATGTGGA GAGCATCCAG TGGCGGGACA TAGTCAGCAG TGACTTTCTC AGCAACATGT
  781 CGATGGACTT CCAGAACCAC CTGGGCAGCT GCCAAAAGTG TGATCCAAGC TGTCCCAATG
  841 GGAGCTGCTG GGGTGCAGGA GAGGAGAACT GCCAGAAACT GACCAAAATC ATCTGTGCCC
  901 AGCAGTGCTC CGGGCGCTGC CGTGGCAAGT CCCCCCAGTG ACTGCTGCCA CAACCAGTGT
  961 GCTGCAGCTG CACAGGCCCC CCGGGGAGAG CGACTGCCTG GTCTGCCGCA AATTCCGAGA
 1021 CGAAGCCACG TGCAAGGACA CCTGCCCCCC CCACTCATGC TCTACACCCC CCACCCACGT
1081 ACAGAATTGA TGGTGCACCC GAAGGGCCAA AATAACAGCC TTTTTGGGGG TGTCA
//
Region: attL2, insert 3' | Primer: pDONR_R | Sequence: RDB15491_A7K8b.seq
>D04925A4_A7K8_2_pDONR_R_D08_11_ABI24.ab1
    1 GGGGACGTCA GGGACGCTAT GACCATGTAA TACGACTCAC TATAGGGGAT ATCAGCTGGA
   61 TGGCAAATAA TGATTTTATT TTGACTGATA GTGACCTGTT CGTTGCAACA AATTGATAAG
  121 CAATGCTTTC TTATAATGCC AACTTTGTAC AAGAAAGCTG GGTCGGCGCG CCCACCCTTT
  181 CATGCTCCAA TAAATTCACT GCTTTGTGGC GCGACCCTTA GGTATTCTGC ATTTTCAGCT
  241 GTGGAGCCCT TAAAGATGCC ATTTGGCTTG GCTTCCTTGG GAAAGAAGTC CTGCTGGTAG
  301 TCAGGGTTGT CCAGGCTAAT TTGGTGGCTG CCTTTCTGGG CCCAGTGGGC AGGGCTGTCG
  361 AATGTGCTGT TGACACAGGT GGGCTGGACA GTGTTGAGAT ACTCGGGGTT GCCCACTGCA
  421 GTGCTGTGGG GGTCCTGGTA GTGTGGGTCT CTGCTGGGCG CGGGGTTCAG AGGCTGATTG
  481 TGATAGACAG GATTCTGCAC AGAGCCAGCG GGCCTTTTGG GAACGGACTG GTTTATGTAT
  541 TCAGGCACTG GGAGGAAGGT GTCGTCTATG CTGTCCTCAG TCAAGGCGCC TGTGGGGTCT
  601 GAGCTGTATC GCTGCAAGAA GCTGTCTTCC TTGATGGGAC AGCTTTGCAG CCCATTTCTA
  661 TCAATGCAAG CCACGGTGGA ATTGTTGCTG GTTGCACTCA GAGAGCTCAG GAGGGGAGTC
  721 CGTGACGTGG AGGGGCTGCT GAAGAAGCCC TGCTGTGGGA TGAGGTACTC GTCGGCATCC
  781 ACCACGTCGT CCATGTCTTC TTCATCCATC AGGGCACGGT AGAAGTTGGA GTCTGTAGGA
  841 CTTGGCAAAT GCATTCTTTC ATCCCCCTGA ATGACAAGGT AGCGCTGGGG GGTCTCGGGC
  901 CATTTTGGAG AATTCGATGA TCAACTCACG GAACTTTGGG CGACTATCTG CGTCTATCAT
  961 CCAGCACTTG ACCATGATCA TGTAGACATC GATGGTACAT ATGGGTGGCT GAGGAGGCGT
 1021 TTCTCCTTTC TCCAGGATGG GAGGAGATCT CGGCTGGGCA AGGATTCCCG CTCATATGAC
1081 TGGAATTCGA AGATCATCAA CCTCCCCAAA ACCAGT
//
Region: insert middle region (C797S, del19) | Primer: EGFR-F5 | Sequence: RDB15491_A7K8c.seq
>D04925A4_A7K8_2_EGFR-F5_D09_12_ABI24.ab1
    1 ACCTTATCGT CATCGCCACT GGGATGGTGG GGGCCCTCCT CTTGCTGCTG GTGGTGGCCC
   61 TGGGGATCGG CCTCTTCATG CGAAGGCGCC ACATCGTTCG GAAGCGCACG CTGCGGAGGC
  121 TGCTGCAGGA GAGGGAGCTT GTGGAGCCTC TTACACCCAG TGGAGAAGCT CCCAACCAAG
  181 CTCTCTTGAG GATCTTGAAG GAAACTGAAT TCAAAAAGAT CAAAGTGCTG GGCTCCGGTG
  241 CGTTCGGCAC GGTGTATAAG GGACTCTGGA TCCCAGAAGG TGAGAAAGTT AAAATTCCCG
  301 TCGCTATCAA GACATCTCCG AAAGCCAACA AGGAAATCCT CGATGAAGCC TACGTGATGG
  361 CCAGCGTGGA CAACCCCCAC GTGTGCCGCC TGCTGGGCAT CTGCCTCACC TCCACCGTGC
  421 AACTCATCAC GCAGCTCATG CCCTTCGGCT CCCTCCTGGA CTATGTCCGG GAACACAAAG
  481 ACAATATTGG CTCCCAGTAC CTGCTCAACT GGTGTGTGCA GATCGCAAAG GGCATGAACT
  541 ACTTGGAGGA CCGTCGCTTG GTGCACCGCG ACCTGGCAGC CAGGAACGTA CTGGTGAAAA
  601 CACCGCAGCA TGTCAAGATC ACAGATTTTG GGCTGGCCAA ACTGCTGGGT GCGGAAGAGA
  661 AAGAATACCA TGCAGAAGGA GGCAAAGTGC CTATCAAGTG GATGGCATTG GAATCAATTT
  721 TACACAGAAT CTATACCCAC CAGAGTGATG TCTGGAGCTA CGGGGTGACC GTTTGGGAGT
  781 TGATGACCTT TGGATCCAAG CCATATGACG GAATCCCTGC CAGCGAGATC TCCTCCATCC
  841 TGGAGAAAGG AGAACGCCTC CCTCAGCCAC CCATATGTAC CATCGATGTC TACATGATCA
  901 TGGTCAAGTG CTGGATGATA GACGCAGATA GTCGCCCAAA GTTCCGTGAG TTGATCATCG
  961 AATTCTCCAA AATGGCCCGA GACCCCCAGC GCTACCCTTG TCATTCACGG GGGATGAAGA
 1021 TGCATTTGCC AAGTCCTACA GACTCTAACT TTCTACCGTG CCCCTGATCA TGGAAGGAGA
1081 CATGGGACGA ACGTTGGATT GGAATTGGCC GGACG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Uchibori, K., Brigatinib combined with anti-EGFR antibody overcomes osimertinib resistance in EGFR-mutated non-small-cell lung cancer. Nat. Commun. 8: 14768 (2017). PMID 28287083.

2019.09.20

GNP_filter3_RDBDEP_html_190816.pl