Please review the QC test results indicated by icon below as well as clone information before placing your order.

pENTER-EGFR T790M/del19

Gateway(R) entry vector of human epidermal growth factor receptor (EGFR), EGFR-tyrosine kinase inhibitor (TKI) resistant and activating mutations.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Uchibori, K et al. Nat. Commun. 8: 14768, 2017)
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	Gateway® Entry clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Uchibori, K et al. Nat. Commun. 8: 14768, 2017)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	Gateway®テクノロジーを用いたエントリークローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15490_A7K8p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: rrnBT1ter_F2 (Pr0531) Region: rrnB T1 ter,attL1,insert 5' Sequence file: RDB15490_A7K8d.seq
>D04925A3_A7K8_1_rrnBT1ter_F2_C04_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN CCTTTCGTTT NNTTTGATGC CTGGCAGTTC CCTACTCTCG    61 CGTTAACGCT AGCATGGATG TTTTCCCAGT CACGACGTTG TAAAACGACG GCCAGTCTTA   121 AGCTCGGGCC CCAAATAATG ATTTTATTTT GACTGATAGT GACCTGTTCG TTGCAACAAA   181 TTGATGAGCA ATGCTTTTTT ATAATGCCAA CTTTGTACAA AAAAGCAGGC TCCGCGGCCG   241 CCCCCTTCAC CATGCGACCC TCCGGGACGG CCGGGGCAGC GCTCCTGGCG CTGCTGGCTG   301 CGCTCTGCCC GGCGAGTCGG GCTCTGGAGG AAAAGAAAGT TTGCCAAGGC ACGAGTAACA   361 AGCTCACGCA GTTGGGCACT TTTGAAGATC ATTTTCTCAG CCTCCAGAGG ATGTTCAATA   421 ACTGTGAGGT GGTCCTTGGG AATTTGGAAA TTACCTATGT GCAGAGGAAT TATGATCTTT   481 CCTTCTTAAA GACCATCCAG GAGGTGGCTG GTTATGTCCT CATTGCCCTC AACACAGTGG   541 AGCGAATTCC TTTGGAAAAC CTGCAGATCA TCAGAGGAAA TATGTACTAC GAAAATTCCT   601 ATGCCTTAGC AGTCTTATCT AACTATGATG CAAATAAAAC CGGACTGAAG GAGCTGCCCA   661 TGAGAAANTT ACAGGAAATC CTGCATGGCG CCGTGCGGTT CAGCAACAAC CCTGCCCTGT   721 GCAATGTGGA GAGCATCCAG TGGCGGGACA TAGTCAGCAG TGACTTTCTC AGCAACATGT   781 CGATGGACTT CCAGAACCAC CTGGGCAGCT GCCAAAAGTG TGNTCCAAGC TGTCCCAATN   841 NNANCTGCTG GGGTGCANGA NANGANAANT GCCAGAAACT GANCAAAATC ATCTGNGCCC   901 AGCAGNGCNC CGGGCGCTGC CNNGGCAAGN CCCCCNANNN NNNGCTGCCA CAACCANNNN 961 NNTGNNNNNN NNCNNNNCCC CNGGNNNNNC NNNNNNNNNN //
Primer: pDONR_R (Pr0071) Region: attL2,insert 3' Sequence file: RDB15490_A7K8e.seq
>D04925A3_A7K8_1_pDONR_R_C08_08_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNCN NTGNNNNTGT AATACGACTC ACTATAGGGG ANATCAGCTG    61 GATGGCAAAT AATGATTTTA TTTTGACTGA TAGTGACCTG TTCGTTGCAA CAAATTGATA   121 AGCAATGCTT TCTTATAATG CCAACTTTGT ACAAGAAAGC TGGGTCGGCG CGCCCACCCT   181 TTCATGCTCC AATAAATTCA CTGCTTTGTG GCGCGACCCT TAGGTATTCT GCATTTTCAG   241 CTGTGGAGCC CTTAAAGATG CCATTTGGCT TGGCTTCCTT GGGAAAGAAG TCCTGCTGGT   301 AGTCAGGGTT GTCCAGGCTA ATTTGGTGGC TGCCTTTCTG GGCCCAGTGG GCAGGGCTGT   361 CGAATGTGCT GTTGACACAG GTGGGCTGGA CAGTGTTGAG ATACTCGGGG TTGCCCACTG   421 CAGTGCTGTG GGGGTCCTGG TAGTGTGGGT CTCTGCTGGG CGCGGGGTTC AGAGGCTGAT   481 TGTGATAGAC AGGATTCTGC ACAGAGCCAG CGGGCCTTTT GGGAACGGAC TGGTTTATGT   541 ATTCAGGCAC TGGGAGGAAG GTGTCGTCTA TGCTGTCCTC AGTCAAGGCG CCTGTGGGGT   601 CTGANCTGTA TCGCTGCAAG AAGCTGTCTT CCTTGATGGG ACAGCTTTGC AGCCCATTTC   661 TATCNATGCA AGCCACGGNG GAATTGTTGC TGGTTGCACT CAGANANCTC NNNANGGGAN   721 TCCGTGANGN GGANGGGCTG CTGNANAANC CCTGCTGTGG GNNGANGNAN TCGNCNGCNT   781 CCNCCNCNNC NTNCNTGNCN NCNNNNTNCN TCNNGNNNNN GNANANNNNN GNANNCNGNN 841 NNANTNNNNN AANGCNTNNN TNNNNNCNNN NNNNNNN //
Primer: EGFR-F5 (Pr0313) Region: insert mid(T790M/del19) Sequence file: RDB15490_A7K8f.seq
>D04925A3_A7K8_1_EGFR-F5_C09_09_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNGGNNG GTGGGGGCCC TCCTCTTGCT GCTGGTGGTG    61 GCCCTGGGGN NCGGCCTCTT CNTGCGAAGG CGCCACATCG TTCGGAAGCG CACGCTGCGG   121 AGGCTGCTGC AGGAGAGGGA GCTTGTGGAG CCTCTTACAC CCAGTGGAGA AGCTCCCAAC   181 CAAGCTCTCT TGAGGATCTT GAAGGAAACT GAATTCAAAA AGATCAAAGT GCTGGGCTCC   241 GGTGCGTTCG GCACGGTGTA TAAGGGACTC TGGATCCCAG AAGGTGAGAA AGTTAAAATT   301 CCCGTCGCTA TCAAGACATC TCCGAAAGCC AACAAGGAAA TCCTCGATGA AGCCTACGTG   361 ATGGCCAGCG TGGACAACCC CCACGTGTGC CGCCTGCTGG GCATCTGCCT CACCTCCACC   421 GTGCAGCTCA TCATGCAGCT CATGCCCTTC GGCTGCCTCC TGGACTATGT CCGGGAACAC   481 AAAGACAATA TTGGCTCCCA GTACCTGCTC AACTGGTGTG TGCAGATCGC AAAGGGCATG   541 AACTACTTGG AGGACCGTCG CTTGGTGCAC CGCGACCTGG CAGCCAGGAA CGTACTGGTG   601 AAAACACCGC AGCATGTCAA GATCACAGAT TTTGGGCTGG CCAAACTGCT GGGTGCGGAA   661 GAGAAAGAAT ACCATGCAGA AGGAGGCAAA GTGCCTATCA AGTGGATGGC ATTGGAATCA   721 ATTTTACACA GAATCTATAC CCACCAGAGT GATGTCTGGA GCTACGGNNT GANCGTTTGG   781 GAGTTGATGA NCTTTGGATC CAAGCCATAT GACGGAATCC CTGCCNGCGA GATCTCCTCC   841 NTCCTNGANA AAGGANAACG CCNCCNTCAG CCNCCCATAT GTANCNTCGA NGNCTACATG   901 ATCATGGNCN ANNGCTGGNT GATAGNNGCN NANAGNCGCC NNAANTTNCN NNNNNNGATC 961 ATCNNNNNNN NNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl