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pENTER-EGFR T790M/del19

Gateway(R) entry vector of human epidermal growth factor receptor (EGFR), EGFR-tyrosine kinase inhibitor (TKI) resistant and activating mutations.

Catalog number RDB15490
Resource name pENTER-EGFR T790M/del19
Clone info. Gateway(R) entry vector of human epidermal growth factor receptor (EGFR) mutant, T790M and deletion in exon 19, EGFR-tyrosine kinase inhibitor (TKI) resistant and activating mutations.
cDNA was obtained from HCC-827 cells. Expression was confirmed by the depositor with western blotting after cloned into pLenti6.3 vector (by LR clonase II reaction).
Vector backbone pENTR (plasmid)
Size of vector backbone 2.6 kb
Selectable markers Kan^r
Gene/insert name human EGFR cDNA
Depositor Katayama, Ryohei |
 
Other clones in our bank human EGFR (NCBI Gene 1956) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Uchibori, K et al. Nat. Commun. 8: 14768, 2017)
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Remarks
Gateway® Entry clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
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RDB15490 pENTER-EGFR T790M/del19 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Uchibori, K., Brigatinib combined with anti-EGFR antibody overcomes osimertinib resistance in EGFR-mutated non-small-cell lung cancer. Nat. Commun. 8: 14768 (2017). PMID 28287083.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15490_A7K8p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Region: rrnB T1 ter, attL1, insert 5' | Primer: rrnBT1ter_F2 | Sequence: RDB15490_A7K8a.seq
>D04925A3_A7K8_1_rrnBT1ter_F2_C04_07_ABI24.ab1
    1 AACCAGATCT TTCGACTGAG CCTTTCGTTT TATTTGATGC CTGGCAGTTC CCTACTCTCG
   61 CGTTAACGCT AGCATGGATG TTTTCCCAGT CACGACGTTG TAAAACGACG GCCAGTCTTA
  121 AGCTCGGGCC CCAAATAATG ATTTTATTTT GACTGATAGT GACCTGTTCG TTGCAACAAA
  181 TTGATGAGCA ATGCTTTTTT ATAATGCCAA CTTTGTACAA AAAAGCAGGC TCCGCGGCCG
  241 CCCCCTTCAC CATGCGACCC TCCGGGACGG CCGGGGCAGC GCTCCTGGCG CTGCTGGCTG
  301 CGCTCTGCCC GGCGAGTCGG GCTCTGGAGG AAAAGAAAGT TTGCCAAGGC ACGAGTAACA
  361 AGCTCACGCA GTTGGGCACT TTTGAAGATC ATTTTCTCAG CCTCCAGAGG ATGTTCAATA
  421 ACTGTGAGGT GGTCCTTGGG AATTTGGAAA TTACCTATGT GCAGAGGAAT TATGATCTTT
  481 CCTTCTTAAA GACCATCCAG GAGGTGGCTG GTTATGTCCT CATTGCCCTC AACACAGTGG
  541 AGCGAATTCC TTTGGAAAAC CTGCAGATCA TCAGAGGAAA TATGTACTAC GAAAATTCCT
  601 ATGCCTTAGC AGTCTTATCT AACTATGATG CAAATAAAAC CGGACTGAAG GAGCTGCCCA
  661 TGAGAAATTT ACAGGAAATC CTGCATGGCG CCGTGCGGTT CAGCAACAAC CCTGCCCTGT
  721 GCAATGTGGA GAGCATCCAG TGGCGGGACA TAGTCAGCAG TGACTTTCTC AGCAACATGT
  781 CGATGGACTT CCAGAACCAC CTGGGCAGCT GCCAAAAGTG TGATCCAAGC TGTCCCAATG
  841 GGAGCTGCTG GGGTGCAGGA GAGGAGAACT GCCAGAAACT GACCAAAATC ATCTGTGCCC
  901 AGCAGTGCTC CGGGCGCTGC CGTGGCAAGT CCCCCCAGTG ACTGCTGCCA CAACCAGTGT
  961 GCTGCAGCTG CACAGGCCCC CCGGGAGAGC GACTGCCTGG TCTGCCGCAA ATTCCGAGAC
 1021 GAAGCCACGT GCAAAGGACA CCTGCCCCCC CCACTCATGC CTCTACAAAC CCCCACCACG
1081 TTAACAGATT GGATGATGAA CCCGGAGGCA AAATAACAAG CCTTTTGGGA TGC
//
Region: attL2, insert 3' | Primer: pDONR_R | Sequence: RDB15490_A7K8b.seq
>D04925A3_A7K8_1_pDONR_R_C08_08_ABI24.ab1
    1 GGCGGACGGC AGGGAACGCT ATGACCATGT AATACGACTC ACTATAGGGG ATATCAGCTG
   61 GATGGCAAAT AATGATTTTA TTTTGACTGA TAGTGACCTG TTCGTTGCAA CAAATTGATA
  121 AGCAATGCTT TCTTATAATG CCAACTTTGT ACAAGAAAGC TGGGTCGGCG CGCCCACCCT
  181 TTCATGCTCC AATAAATTCA CTGCTTTGTG GCGCGACCCT TAGGTATTCT GCATTTTCAG
  241 CTGTGGAGCC CTTAAAGATG CCATTTGGCT TGGCTTCCTT GGGAAAGAAG TCCTGCTGGT
  301 AGTCAGGGTT GTCCAGGCTA ATTTGGTGGC TGCCTTTCTG GGCCCAGTGG GCAGGGCTGT
  361 CGAATGTGCT GTTGACACAG GTGGGCTGGA CAGTGTTGAG ATACTCGGGG TTGCCCACTG
  421 CAGTGCTGTG GGGGTCCTGG TAGTGTGGGT CTCTGCTGGG CGCGGGGTTC AGAGGCTGAT
  481 TGTGATAGAC AGGATTCTGC ACAGAGCCAG CGGGCCTTTT GGGAACGGAC TGGTTTATGT
  541 ATTCAGGCAC TGGGAGGAAG GTGTCGTCTA TGCTGTCCTC AGTCAAGGCG CCTGTGGGGT
  601 CTGAGCTGTA TCGCTGCAAG AAGCTGTCTT CCTTGATGGG ACAGCTTTGC AGCCCATTTC
  661 TATCAATGCA AGCCACGGTG GAATTGTTGC TGGTTGCACT CAGAGAGCTC ACGAGGGGAG
  721 TCCGTGACGT GGAGGGGCTG CTGAAGAAGC CCTGCTGTGG GATGAGGTAC TCGTCGGCAT
  781 CCACCACGTC GTCCATGTCT TCTTCATCCA TCAGGGCACG GTAGAAGTTG GGAGTCTGTA
  841 GGACTTGGCA AATGCATTCT TTCATCCCCC TGAATGACAG GTAGCGCTGG GGGTCTCGGG
  901 CCATTTGGAG AATTCGATGA TCACTCACGG ACTTGGGGCG ACTATCTGCG TCTATCATCC
  961 AGCACTGAAC AATGATCATG TAGAACATTC GAATGTACAT ATGGGTGGCC TGAGGAGCGT
 1021 CTCTTCTCAG GAATGAGAAT CCCGCTTGCA CGATCGTCAA TGGCTGGAAT CGAAGATCAT
1081 TCAAACT
//
Region: insert middle region (T790M, del19) | Primer: EGFR-F5 | Sequence: RDB15490_A7K8c.seq
>D04925A3_A7K8_1_EGFR-F5_C09_09_ABI24.ab1
    1 AAAATAAATC CGGTCATCGC CACTGGGATG GTGGGGGCCC TCCTCTTGCT GCTGGTGGTG
   61 GCCCTGGGGA TCGGCCTCTT CATGCGAAGG CGCCACATCG TTCGGAAGCG CACGCTGCGG
  121 AGGCTGCTGC AGGAGAGGGA GCTTGTGGAG CCTCTTACAC CCAGTGGAGA AGCTCCCAAC
  181 CAAGCTCTCT TGAGGATCTT GAAGGAAACT GAATTCAAAA AGATCAAAGT GCTGGGCTCC
  241 GGTGCGTTCG GCACGGTGTA TAAGGGACTC TGGATCCCAG AAGGTGAGAA AGTTAAAATT
  301 CCCGTCGCTA TCAAGACATC TCCGAAAGCC AACAAGGAAA TCCTCGATGA AGCCTACGTG
  361 ATGGCCAGCG TGGACAACCC CCACGTGTGC CGCCTGCTGG GCATCTGCCT CACCTCCACC
  421 GTGCAGCTCA TCATGCAGCT CATGCCCTTC GGCTGCCTCC TGGACTATGT CCGGGAACAC
  481 AAAGACAATA TTGGCTCCCA GTACCTGCTC AACTGGTGTG TGCAGATCGC AAAGGGCATG
  541 AACTACTTGG AGGACCGTCG CTTGGTGCAC CGCGACCTGG CAGCCAGGAA CGTACTGGTG
  601 AAAACACCGC AGCATGTCAA GATCACAGAT TTTGGGCTGG CCAAACTGCT GGGTGCGGAA
  661 GAGAAAGAAT ACCATGCAGA AGGAGGCAAA GTGCCTATCA AGTGGATGGC ATTGGAATCA
  721 ATTTTACACA GAATCTATAC CCACCAGAGT GATGTCTGGA GCTACGGGGT GACCGTTTGG
  781 GAGTTGATGA CCTTTGGATC CAAGCCATAT GACGGAATCC CTGCCAGCGA GATCTCCTCC
  841 ATCCTGGAGA AAGGAGAACG CCTCCCTCAG CCACCCATAT GTACCATCGA TGTCTACATG
  901 ATCATGGTCA AGTGCTGGAT GATAGACGCA GATAGTCGCC CAAAGTTCCG TGAGTTGATC
  961 ATCGAATTCT CCAAAATGGC CCGAGACCCC CCAGCGCTAC CCTTTGTCAT TCAGGGGGAT
 1021 GAAAGAATGC ATTTGCCCAA AGTCCCTACA GACCTCGACC TTCTACCGTG CCCTTGATGT
1081 ATGAAGAAGG ACATGGAACG GAACGGTGGT GGAATGGCCC GA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Uchibori, K., Brigatinib combined with anti-EGFR antibody overcomes osimertinib resistance in EGFR-mutated non-small-cell lung cancer. Nat. Commun. 8: 14768 (2017). PMID 28287083.

2019.09.20

GNP_filter3_RDBDEP_html_190816.pl