Gateway(R) entry vector of human epidermal growth factor receptor (EGFR), EGFR-activating mutation.
Catalog number | RDB15489 |
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Resource name | pENTER-EGFR Del19 |
Clone info. | Gateway(R) entry vector of human epidermal growth factor receptor (EGFR) mutant, deletion in exon 19, EGFR-activating mutation. cDNA was obtained from HCC-827 cells. Expression was confirmed by the depositor with western blotting after cloned into pLenti6.3 vector (by LR clonase II reaction). |
Vector backbone | pENTR (plasmid) |
Size of vector backbone | 2.6 kb |
Selectable markers | Kan^r |
Gene/insert name | human EGFR cDNA |
Depositor | Katayama, Ryohei | |
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Other clones in our bank | human EGFR (NCBI Gene 1956) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Uchibori, K et al. Nat. Commun. 8: 14768, 2017) |
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Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
Remarks | Gateway® Entry clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC. |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB15489 | pENTER-EGFR Del19 | DNA solution |
Electronic file
Original reference
original | Uchibori, K., Brigatinib combined with anti-EGFR antibody overcomes osimertinib resistance in EGFR-mutated non-small-cell lung cancer. Nat. Commun. 8: 14768 (2017). PMID 28287083. |
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Further references such as user reports and related articles (go to bottom)
Featured content
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
Test sheet | RDB15489_A7K8p1-1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
Region: rrnB T1 ter, attL1, insert 5' | Primer: rrnBT1ter_F2 | Sequence: RDB15489_A7K8a.seq |
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>D04925A2_A7K8_2_rrnBT1ter_F2_B04_04_ABI24.ab1 1 AACGCCAAGG TCTTCCGACT GAGCCTTTCG TTTTATTTGA TGCCTGGCAG TTCCCTACTC 61 TCGCGTTAAC GCTAGCATGG ATGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTC 121 TTAAGCTCGG GCCCCAAATA ATGATTTTAT TTTGACTGAT AGTGACCTGT TCGTTGCAAC 181 AAATTGATGA GCAATGCTTT TTTATAATGC CAACTTTGTA CAAAAAAGCA GGCTCCGCGG 241 CCGCCCCCTT CACCATGCGA CCCTCCGGGA CGGCCGGGGC AGCGCTCCTG GCGCTGCTGG 301 CTGCGCTCTG CCCGGCGAGT CGGGCTCTGG AGGAAAAGAA AGTTTGCCAA GGCACGAGTA 361 ACAAGCTCAC GCAGTTGGGC ACTTTTGAAG ATCATTTTCT CAGCCTCCAG AGGATGTTCA 421 ATAACTGTGA GGTGGTCCTT GGGAATTTGG AAATTACCTA TGTGCAGAGG AATTATGATC 481 TTTCCTTCTT AAAGACCATC CAGGAGGTGG CTGGTTATGT CCTCATTGCC CTCAACACAG 541 TGGAGCGAAT TCCTTTGGAA AACCTGCAGA TCATCAGAGG AAATATGTAC TACGAAAATT 601 CCTATGCCTT AGCAGTCTTA TCTAACTATG ATGCAAATAA AACCGGACTG AAGGAGCTGC 661 CCATGAGAAA TTTACAGGAA ATCCTGCATG GCGCCGTGCG GTTCAGCAAC AACCCTGCCC 721 TGTGCAATGT GGAGAGCATC CAGTGGCGGG ACATAGTCAG CAGTGACTTT CTCAGCAACA 781 TGTCGATGGA CTTCCAGAAC CACCTGGGCA GCTGCCAAAA GTGTGATCCA AGCTGTCCCA 841 ATGGGAGCTG CTGGGGTGCA GGAGAGGAGA ACTGCCAGAA ACTGACCAAA ATCATCTGTG 901 CCCAGCAGTG CTCCGGGCGC TGCCGTGGCA AGTCCCCCCA GTGACTGCTG CCACAACCAG 961 TGTGCTGCAG GCTGCACAGG CCCCCCGGGA GAGCGACTGC CCTGGTCTGC CCGCAAATTC 1021 CGAGACGAAA GCCACGTGCA AAGGACACCC TGCCCCCCCC CACTCATGCT TCTACAAACC 1081 CCCACCACGT ACAAGATTGG ATGATGAAAT CCCGAAGGTC AAAATAACAG GCCTTTTG // |
Region: attL2, insert 3' | Primer: pDONR_R | Sequence: RDB15489_A7K8b.seq |
>D04925A2_A7K8_2_EGFR-F5_B09_06_ABI24.ab1 1 AACGTAATCG TCATCGCCAC TGGGATGGTG GGGGCCCTCC TCTTGCTGCT GGTGGTGGCC 61 CTGGGGATCG GCCTCTTCAT GCGAAGGCGC CACATCGTTC GGAAGCGCAC GCTGCGGAGG 121 CTGCTGCAGG AGAGGGAGCT TGTGGAGCCT CTTACACCCA GTGGAGAAGC TCCCAACCAA 181 GCTCTCTTGA GGATCTTGAA GGAAACTGAA TTCAAAAAGA TCAAAGTGCT GGGCTCCGGT 241 GCGTTCGGCA CGGTGTATAA GGGACTCTGG ATCCCAGAAG GTGAGAAAGT TAAAATTCCC 301 GTCGCTATCA AGACATCTCC GAAAGCCAAC AAGGAAATCC TCGATGAAGC CTACGTGATG 361 GCCAGCGTGG ACAACCCCCA CGTGTGCCGC CTGCTGGGCA TCTGCCTCAC CTCCACCGTG 421 CAACTCATCA CGCAGCTCAT GCCCTTCGGC TGCCTCCTGG ACTATGTCCG GGAACACAAA 481 GACAATATTG GCTCCCAGTA CCTGCTCAAC TGGTGTGTGC AGATCGCAAA GGGCATGAAC 541 TACTTGGAGG ACCGTCGCTT GGTGCACCGC GACCTGGCAG CCAGGAACGT ACTGGTGAAA 601 ACACCGCAGC ATGTCAAGAT CACAGATTTT GGGCTGGCCA AACTGCTGGG TGCGGAAGAG 661 AAAGAATACC ATGCAGAAGG AGGCAAAGTG CCTATCAAGT GGATGGCATT GGAATCAATT 721 TTACACAGAA TCTATACCCA CCAGAGTGAT GTCTGGAGCT ACGGGGTGAC CGTTTGGGAG 781 TTGATGACCT TTGGATCCAA GCCATATGAC GGAATCCCTG CCAGCGAGAT CTCCTCCATC 841 CTGGAGAAAG GAGAACGCCT CCCTCAGCCA CCCATATGTA CCATCGATGT CTACATGATC 901 ATGGTCAAGT GCTGGATGAT AGACGCAGAT AGTCGCCCAA AGTTCCGTGA GTTGATCATC 961 GAAATTCTCC AAAATGGCCC GAGACCCCCC AGCGCTACCC TTTGTCATTC AGGGGGATGA 1021 AGAATGCATT TGCCAGTCCC TACAGACTCG ACCTTCCTAC GTGCCTGATG TATGAAGAAG 1081 ACATTGACGA ACGTGAATGG ATGACCGACC GAGGT // |
Region: insert middle region (del19) | Primer: EGFR-F5 | Sequence: RDB15489_A7K8c.seq |
>D04925A2_A7K8_2_EGFR-F5_B09_06_ABI24.ab1 1 AACGTAATCG TCATCGCCAC TGGGATGGTG GGGGCCCTCC TCTTGCTGCT GGTGGTGGCC 61 CTGGGGATCG GCCTCTTCAT GCGAAGGCGC CACATCGTTC GGAAGCGCAC GCTGCGGAGG 121 CTGCTGCAGG AGAGGGAGCT TGTGGAGCCT CTTACACCCA GTGGAGAAGC TCCCAACCAA 181 GCTCTCTTGA GGATCTTGAA GGAAACTGAA TTCAAAAAGA TCAAAGTGCT GGGCTCCGGT 241 GCGTTCGGCA CGGTGTATAA GGGACTCTGG ATCCCAGAAG GTGAGAAAGT TAAAATTCCC 301 GTCGCTATCA AGACATCTCC GAAAGCCAAC AAGGAAATCC TCGATGAAGC CTACGTGATG 361 GCCAGCGTGG ACAACCCCCA CGTGTGCCGC CTGCTGGGCA TCTGCCTCAC CTCCACCGTG 421 CAACTCATCA CGCAGCTCAT GCCCTTCGGC TGCCTCCTGG ACTATGTCCG GGAACACAAA 481 GACAATATTG GCTCCCAGTA CCTGCTCAAC TGGTGTGTGC AGATCGCAAA GGGCATGAAC 541 TACTTGGAGG ACCGTCGCTT GGTGCACCGC GACCTGGCAG CCAGGAACGT ACTGGTGAAA 601 ACACCGCAGC ATGTCAAGAT CACAGATTTT GGGCTGGCCA AACTGCTGGG TGCGGAAGAG 661 AAAGAATACC ATGCAGAAGG AGGCAAAGTG CCTATCAAGT GGATGGCATT GGAATCAATT 721 TTACACAGAA TCTATACCCA CCAGAGTGAT GTCTGGAGCT ACGGGGTGAC CGTTTGGGAG 781 TTGATGACCT TTGGATCCAA GCCATATGAC GGAATCCCTG CCAGCGAGAT CTCCTCCATC 841 CTGGAGAAAG GAGAACGCCT CCCTCAGCCA CCCATATGTA CCATCGATGT CTACATGATC 901 ATGGTCAAGT GCTGGATGAT AGACGCAGAT AGTCGCCCAA AGTTCCGTGA GTTGATCATC 961 GAAATTCTCC AAAATGGCCC GAGACCCCCC AGCGCTACCC TTTGTCATTC AGGGGGATGA 1021 AGAATGCATT TGCCAGTCCC TACAGACTCG ACCTTCCTAC GTGCCTGATG TATGAAGAAG 1081 ACATTGACGA ACGTGAATGG ATGACCGACC GAGGT // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Uchibori, K., Brigatinib combined with anti-EGFR antibody overcomes osimertinib resistance in EGFR-mutated non-small-cell lung cancer. Nat. Commun. 8: 14768 (2017). PMID 28287083. |
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2019.09.20
GNP_filter3_RDBDEP_html_190816.pl