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pENTER-EGFR Del19

Gateway(R) entry vector of human epidermal growth factor receptor (EGFR), EGFR-activating mutation.

Catalog number RDB15489
Resource name pENTER-EGFR Del19
Clone info. Gateway(R) entry vector of human epidermal growth factor receptor (EGFR) mutant, deletion in exon 19, EGFR-activating mutation.
cDNA was obtained from HCC-827 cells. Expression was confirmed by the depositor with western blotting after cloned into pLenti6.3 vector (by LR clonase II reaction).
Vector backbone pENTR (plasmid)
Size of vector backbone 2.6 kb
Selectable markers Kan^r
Gene/insert name human EGFR cDNA
Depositor Katayama, Ryohei |
 
Other clones in our bank human EGFR (NCBI Gene 1956) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Uchibori, K et al. Nat. Commun. 8: 14768, 2017)
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Remarks
Gateway® Entry clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
Distribution information (domestic users) [open/close]


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RDB15489 pENTER-EGFR Del19 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Uchibori, K., Brigatinib combined with anti-EGFR antibody overcomes osimertinib resistance in EGFR-mutated non-small-cell lung cancer. Nat. Commun. 8: 14768 (2017). PMID 28287083.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15489_A7K8p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Region: rrnB T1 ter, attL1, insert 5' | Primer: rrnBT1ter_F2 | Sequence: RDB15489_A7K8a.seq
>D04925A2_A7K8_2_rrnBT1ter_F2_B04_04_ABI24.ab1
    1 AACGCCAAGG TCTTCCGACT GAGCCTTTCG TTTTATTTGA TGCCTGGCAG TTCCCTACTC
   61 TCGCGTTAAC GCTAGCATGG ATGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTC
  121 TTAAGCTCGG GCCCCAAATA ATGATTTTAT TTTGACTGAT AGTGACCTGT TCGTTGCAAC
  181 AAATTGATGA GCAATGCTTT TTTATAATGC CAACTTTGTA CAAAAAAGCA GGCTCCGCGG
  241 CCGCCCCCTT CACCATGCGA CCCTCCGGGA CGGCCGGGGC AGCGCTCCTG GCGCTGCTGG
  301 CTGCGCTCTG CCCGGCGAGT CGGGCTCTGG AGGAAAAGAA AGTTTGCCAA GGCACGAGTA
  361 ACAAGCTCAC GCAGTTGGGC ACTTTTGAAG ATCATTTTCT CAGCCTCCAG AGGATGTTCA
  421 ATAACTGTGA GGTGGTCCTT GGGAATTTGG AAATTACCTA TGTGCAGAGG AATTATGATC
  481 TTTCCTTCTT AAAGACCATC CAGGAGGTGG CTGGTTATGT CCTCATTGCC CTCAACACAG
  541 TGGAGCGAAT TCCTTTGGAA AACCTGCAGA TCATCAGAGG AAATATGTAC TACGAAAATT
  601 CCTATGCCTT AGCAGTCTTA TCTAACTATG ATGCAAATAA AACCGGACTG AAGGAGCTGC
  661 CCATGAGAAA TTTACAGGAA ATCCTGCATG GCGCCGTGCG GTTCAGCAAC AACCCTGCCC
  721 TGTGCAATGT GGAGAGCATC CAGTGGCGGG ACATAGTCAG CAGTGACTTT CTCAGCAACA
  781 TGTCGATGGA CTTCCAGAAC CACCTGGGCA GCTGCCAAAA GTGTGATCCA AGCTGTCCCA
  841 ATGGGAGCTG CTGGGGTGCA GGAGAGGAGA ACTGCCAGAA ACTGACCAAA ATCATCTGTG
  901 CCCAGCAGTG CTCCGGGCGC TGCCGTGGCA AGTCCCCCCA GTGACTGCTG CCACAACCAG
  961 TGTGCTGCAG GCTGCACAGG CCCCCCGGGA GAGCGACTGC CCTGGTCTGC CCGCAAATTC
 1021 CGAGACGAAA GCCACGTGCA AAGGACACCC TGCCCCCCCC CACTCATGCT TCTACAAACC
1081 CCCACCACGT ACAAGATTGG ATGATGAAAT CCCGAAGGTC AAAATAACAG GCCTTTTG
//
Region: attL2, insert 3' | Primer: pDONR_R | Sequence: RDB15489_A7K8b.seq
>D04925A2_A7K8_2_EGFR-F5_B09_06_ABI24.ab1
    1 AACGTAATCG TCATCGCCAC TGGGATGGTG GGGGCCCTCC TCTTGCTGCT GGTGGTGGCC
   61 CTGGGGATCG GCCTCTTCAT GCGAAGGCGC CACATCGTTC GGAAGCGCAC GCTGCGGAGG
  121 CTGCTGCAGG AGAGGGAGCT TGTGGAGCCT CTTACACCCA GTGGAGAAGC TCCCAACCAA
  181 GCTCTCTTGA GGATCTTGAA GGAAACTGAA TTCAAAAAGA TCAAAGTGCT GGGCTCCGGT
  241 GCGTTCGGCA CGGTGTATAA GGGACTCTGG ATCCCAGAAG GTGAGAAAGT TAAAATTCCC
  301 GTCGCTATCA AGACATCTCC GAAAGCCAAC AAGGAAATCC TCGATGAAGC CTACGTGATG
  361 GCCAGCGTGG ACAACCCCCA CGTGTGCCGC CTGCTGGGCA TCTGCCTCAC CTCCACCGTG
  421 CAACTCATCA CGCAGCTCAT GCCCTTCGGC TGCCTCCTGG ACTATGTCCG GGAACACAAA
  481 GACAATATTG GCTCCCAGTA CCTGCTCAAC TGGTGTGTGC AGATCGCAAA GGGCATGAAC
  541 TACTTGGAGG ACCGTCGCTT GGTGCACCGC GACCTGGCAG CCAGGAACGT ACTGGTGAAA
  601 ACACCGCAGC ATGTCAAGAT CACAGATTTT GGGCTGGCCA AACTGCTGGG TGCGGAAGAG
  661 AAAGAATACC ATGCAGAAGG AGGCAAAGTG CCTATCAAGT GGATGGCATT GGAATCAATT
  721 TTACACAGAA TCTATACCCA CCAGAGTGAT GTCTGGAGCT ACGGGGTGAC CGTTTGGGAG
  781 TTGATGACCT TTGGATCCAA GCCATATGAC GGAATCCCTG CCAGCGAGAT CTCCTCCATC
  841 CTGGAGAAAG GAGAACGCCT CCCTCAGCCA CCCATATGTA CCATCGATGT CTACATGATC
  901 ATGGTCAAGT GCTGGATGAT AGACGCAGAT AGTCGCCCAA AGTTCCGTGA GTTGATCATC
  961 GAAATTCTCC AAAATGGCCC GAGACCCCCC AGCGCTACCC TTTGTCATTC AGGGGGATGA
 1021 AGAATGCATT TGCCAGTCCC TACAGACTCG ACCTTCCTAC GTGCCTGATG TATGAAGAAG
1081 ACATTGACGA ACGTGAATGG ATGACCGACC GAGGT
//
Region: insert middle region (del19) | Primer: EGFR-F5 | Sequence: RDB15489_A7K8c.seq
>D04925A2_A7K8_2_EGFR-F5_B09_06_ABI24.ab1
    1 AACGTAATCG TCATCGCCAC TGGGATGGTG GGGGCCCTCC TCTTGCTGCT GGTGGTGGCC
   61 CTGGGGATCG GCCTCTTCAT GCGAAGGCGC CACATCGTTC GGAAGCGCAC GCTGCGGAGG
  121 CTGCTGCAGG AGAGGGAGCT TGTGGAGCCT CTTACACCCA GTGGAGAAGC TCCCAACCAA
  181 GCTCTCTTGA GGATCTTGAA GGAAACTGAA TTCAAAAAGA TCAAAGTGCT GGGCTCCGGT
  241 GCGTTCGGCA CGGTGTATAA GGGACTCTGG ATCCCAGAAG GTGAGAAAGT TAAAATTCCC
  301 GTCGCTATCA AGACATCTCC GAAAGCCAAC AAGGAAATCC TCGATGAAGC CTACGTGATG
  361 GCCAGCGTGG ACAACCCCCA CGTGTGCCGC CTGCTGGGCA TCTGCCTCAC CTCCACCGTG
  421 CAACTCATCA CGCAGCTCAT GCCCTTCGGC TGCCTCCTGG ACTATGTCCG GGAACACAAA
  481 GACAATATTG GCTCCCAGTA CCTGCTCAAC TGGTGTGTGC AGATCGCAAA GGGCATGAAC
  541 TACTTGGAGG ACCGTCGCTT GGTGCACCGC GACCTGGCAG CCAGGAACGT ACTGGTGAAA
  601 ACACCGCAGC ATGTCAAGAT CACAGATTTT GGGCTGGCCA AACTGCTGGG TGCGGAAGAG
  661 AAAGAATACC ATGCAGAAGG AGGCAAAGTG CCTATCAAGT GGATGGCATT GGAATCAATT
  721 TTACACAGAA TCTATACCCA CCAGAGTGAT GTCTGGAGCT ACGGGGTGAC CGTTTGGGAG
  781 TTGATGACCT TTGGATCCAA GCCATATGAC GGAATCCCTG CCAGCGAGAT CTCCTCCATC
  841 CTGGAGAAAG GAGAACGCCT CCCTCAGCCA CCCATATGTA CCATCGATGT CTACATGATC
  901 ATGGTCAAGT GCTGGATGAT AGACGCAGAT AGTCGCCCAA AGTTCCGTGA GTTGATCATC
  961 GAAATTCTCC AAAATGGCCC GAGACCCCCC AGCGCTACCC TTTGTCATTC AGGGGGATGA
 1021 AGAATGCATT TGCCAGTCCC TACAGACTCG ACCTTCCTAC GTGCCTGATG TATGAAGAAG
1081 ACATTGACGA ACGTGAATGG ATGACCGACC GAGGT
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Uchibori, K., Brigatinib combined with anti-EGFR antibody overcomes osimertinib resistance in EGFR-mutated non-small-cell lung cancer. Nat. Commun. 8: 14768 (2017). PMID 28287083.

2019.09.20

GNP_filter3_RDBDEP_html_190816.pl