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pcDNA3.1(+)_Flag-BATF2 (#RDB15468)

Expression vector of mouse basic leucine zipper transcription factor, ATF-like 2 (BATF2),FLAG-tag.

Clone info. Expression vector of mouse basic leucine zipper transcription factor, ATF-like 2 (BATF2), tagged with FLAG at N-terminal.
Comment Expression was confirmed by the depositor with immunoprecipitation.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Mouse Batf2 cDNA
Depositor|Developer Takeda, Kiyoshi |
 

Distribution information

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Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kitada, S et al. L. Exp. Med. 214 (5) : 1313-1331, 2017). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する（Kitada, S et al. L. Exp. Med. 214 (5) : 1313-1331, 2017）。非営利機関が非営利目的の教育・研究用に用いる場合以外は、大阪大学と別途MTAを締結すること。

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RDB15468 pcDNA3.1(+)_Flag-BATF2 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1(+)_Flag-BATF2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15468).

Reference section:

Kitada, S., Kayama, H., Okuzaki, D., Koga, R., Kobayashi, M., Arima, Y., Kumanogoh, A., Murakami, M., Ikawa, M., Takeda, K., BATF2 inhibits immunopathological Th17 responses by suppressing Il23a expression during Trypanosoma cruzi infection. J. Exp. Med. 214 (5): 1313-1331 (2017). PMID 28356392. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15468_A7Jop1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB15468_A7Joa.seq check
>D04995A2_A7Jo_3_CMV_Forward_E01_05_ABI08.ab1
    1 CTTTCACTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
  121 CTCACTATAG GGAGACCCAA GCTGGCTAGG TCGACACCAC CATGGACTAC AAGGACGACG
  181 ATGACAAGGG ATCCCAACTC TGTGGGAGTA GCGAGCTGCT GACTGAGACA GACCTTGGGG
  241 AGTCTCAGAA ACAGCTGAAG AAGAAGCAGA AGAACCGAGT GGCTGCCCAG CGCAGCCGGC
  301 AGAAGCACAC CAGTAAGGCG GACGCTCTGC ACCAGCAGCA CGAATCCTTG GAGAAACAGA
  361 ACCACGCCCT TCGGAAGGAG ATCCAGGCCT TGCAGACTGA GCTGGCAGGG TGGGGCCGGA
  421 CACTGCACTT GCATGAACGC CTGTGCAGAG TGGACTGTGA TCCCTGCCCT GTTCTGCTGC
  481 CCTCTGGATG CCCAATCCAG GCCAAGCAAC CCTCAGGACA ACCTGCCCCT CTTGGATACA
  541 ATGGCTGCCA GGAACAACTG GGCCTGTTCC AGACTCCGGG CTCATCTCCC CGAGCCCAGC
  601 ATCTCTCTCC AGGTCCATGC TCTCATGAGT CTCCTGGCCT CCTCCCTTCC CTCCTGCCTT
  661 CACTGGCCTT TGACCCTCTC ATGGTGAGGA CACCTCTAGC CCAGCTCTCC CCCAGCCCTG
  721 TCCTGTCTGC ATCATCACCT TCTGGTTCCA GCCTGCTGGG GTCTTTCTCT AAGCTTGATC
  781 CCCTCATACC CAGCCCCCAA GACCAACTGG CCCCACCACA ACCCCTCAGG CAGGAGCAGC
  841 CCACCAGTGG GAGGCTGGCA TCCTCTGACT CTCCAGCTGC TCTGGGACCA GAGTGTTCAC
  901 AGAACAGAGA GCATTTGCCG GCACTGCCTG GCTCCTCCAC TCATTGGCAG AAGTCATCTG
  961 TGGCCCCTAG CCCACAGGCC CTCATGGGCC TTCCCCCCTG CTTTCTTCTG CAAAAGTCCA
 1021 CTTTCTAGGG CGGGCCCGCG TCGACAGCTT TAAGTTTAAA CCGCTGATCC AGCTCGACCT
 1081 GTGCCGTTCT AGATGCAGGC ATCTGATGAT TGGCCCCGTC CCCCGAGCCT TCCTTGGAGC
//
Primer: BGH_rev2
Sequence file: RDB15468_A7Job.seq check
>D04995A2_A7Jo_3_BGH_rev2_F01_06_ABI08.ab1
    1 CAACGGGTTA GGAAGGCACG GGGGAGGGGC AACAACAGAT GGCTGGCAAC TAGAAGGCAC
   61 AGTCGAGGCT GATCAGCGGT TTAAACTTAA GCTGTCGACG CGGCCGCCTA GAAGTGGACT
  121 TTTGCAGAAG AAAGCAGGGG GAAGGCCATG AGGGCCTGTG GGCTAGGGGC CACAGATGAC
  181 TTCTGCCAAT GAGTGGAGGA GCCAGGCAGT GCCGGCAAAT GCTCTCTGTT CTGTGAACAC
  241 TCTGGTCCCA GAGCAGCTGG AGAGTCAGAG GATGCCAGCC TCCCACTGGT GGGCTGCTCC
  301 TGCCTGAGGG GTTGTGGTGG GGCCAGTTGG TCTTGGGGGC TGGGTATGAG GGGATCAAGC
  361 TTAGAGAAAG ACCCCAGCAG GCTGGAACCA GAAGGTGATG ATGCAGACAG GACAGGGCTG
  421 GGGGAGAGCT GGGCTAGAGG TGTCCTCACC ATGAGAGGGT CAAAGGCCAG TGAAGGCAGG
  481 AGGGAAGGGA GGAGGCCAGG AGACTCATGA GAGCATGGAC CTGGAGAGAG ATGCTGGGCT
  541 CGGGGAGATG AGCCCGGAGT CTGGAACAGG CCCAGTTGTT CCTGGCAGCC ATTGTATCCA
  601 AGAGGGGCAG GTTGTCCTGA GGGTTGCTTG GCCTGGATTG GGCATCCAGA GGGCAGCAGA
  661 ACAGGGCAGG GATCACAGTC CACTCTGCAC AGGCGTTCAT GCAAGTGCAG TGTCCGGCCC
  721 CACCCTGCCA GCTCAGTCTG CAAGGCCTGG ATCTCCTTCC GAAGGGCGTG GTTCTGTTTC
  781 TCCAAGGATT CGTGCTGCTG GTGCAGAGCG TCCGCCTTAC TGGTGTGCTT CTGCCGGCTG
  841 CGCTGGGCAG CCACTCGGTT CTTCTGCTTC TTCTTCAGCT GTTTCTGAGA CTCCCCAAGG
  901 TCTGTCTCAG TCAGCAGCTC GCTACTCCCA CAGAGTTGGG GATCCCTTGT CATCGTCGTC
  961 CTTGTAGTCC ATGGGTGGTG TCGACCCTAG CCCAGCTTGG GGTCTTCCCT ATAGTGAGTC
 1021 GTATTAATTT TCGAATAAGC CAAGTAGCAG TGGGGTTTCT TCTAGTTAAG CCAGGAGAGC
 1081 CTCCTGCTTT AATAATAGGA CTTCCCACCG TAACACGCCC GTAACTGCCC AATTATGGCG
1141 GTTCCCAATT GAGGGGGCCG GA
//
Primer: SV40pro_F
Sequence file: RDB15468_A7Joc.seq check
>D04995A2_A7Jo_3_SV40pro_F_G01_07_ABI08.ab1
    1 ACTGGTCCTG GACATTCAGA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA
   61 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG
  121 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
  181 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
  241 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
  301 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
  361 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
  421 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
  481 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
  541 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
  601 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG
  661 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
  721 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT
  781 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGGCTGA
  841 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
  901 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGGTTC GAAATGACCG ACCAAGCGAC
  961 GCCCAAACCT GCCCATCACG AGATTTCGAT TCCCACCCGC CGCCCTTTCT ATGAAAGGTT
 1021 GGCTTTCGGA ATCGTTTTCC CGGGAACGCC GCTGGATGAT CCCTCCAGCG CGTGATCTCA
1081 TGCTGAGCTC TCGCCCAACC CCACTTGTTA TTGCAGCCTA ATCATGGTTA CAAATT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kitada, S., BATF2 inhibits immunopathological Th17 responses by suppressing Il23a expression during Trypanosoma cruzi infection. J. Exp. Med. 214 (5): 1313-1331 (2017). PMID 28356392. [link to RRC of NBRP]