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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGAL3-IL23 promoter (#RDB15467)

Luciferase reporter of the mouse interleukin 23 alpha subunit (IL23 alpha) promote.

Clone info. Luciferase reporter of the mouse interleukin 23, alpha subunit (IL23 alpha) promoter was inserted. Genomic DNA (128,297,963-128,298,387nt of NC_000076.6) corresponding to 1 to 122nt of NM_031252.2 and 0.3 kb up-stream was cloned.
Comment Expression was confirmed by the depositor with luciferase assay.
Vector backbone pGAL3 (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Gene/insert name Mouse Il23a genomic DNA
Depositor|Developer Takeda, Kiyoshi |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kitada, S et al. L. Exp. Med. 214 (5) : 1313-1331, 2017). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
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提供依頼書  [依頼書の記入例 ]
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手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する（Kitada, S et al. L. Exp. Med. 214 (5) : 1313-1331, 2017）。非営利機関が非営利目的の教育・研究用に用いる場合以外は、大阪大学と別途MTAを締結すること。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15467 pGAL3-IL23 promoter DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGAL3-IL23 promoter was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15467).

Reference section:

Kitada, S., Kayama, H., Okuzaki, D., Koga, R., Kobayashi, M., Arima, Y., Kumanogoh, A., Murakami, M., Ikawa, M., Takeda, K., BATF2 inhibits immunopathological Th17 responses by suppressing Il23a expression during Trypanosoma cruzi infection. J. Exp. Med. 214 (5): 1313-1331 (2017). PMID 28356392. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15467_A7Jop1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: PGL4-417F
Sequence file: RDB15467_A7Joa.seq check
>D04995A1_A7Jo_3_pGL4-4174F_A01_01_ABI08.ab1
    1 GATGGGGTCA GACATTTCTC TATCGATAGG TACCGAGCTC TTACGCGTGC TAGCCCGGGC
   61 TCGAGATTAC ACAGGAAGGA ACTGGAGATA TTCATTTAAA TGTCCATTAC TTCCCTGCCC
  121 CTTCTGCAGT CTTCTGTGTT CCTGGAGAGC ATGACACATG TGTCCCGACC TAGGCCTCTA
  181 GCCACAACAA CCTCACCACT CATTTCCCCT GGAACTGAAG CGGCATACCT GGGCTCTCCA
  241 AAGAGGGAGA TGATGTAGGG AGGGGAATCC CACCTGCTCT GAGTCACCTG CTAGTGTGAA
  301 GGGCGGGCCT TACAGTGCAG GGACCTTAAA AAGAGACTGG GAGACAAAAG GAAGAAATCA
  361 GGAAGCCGCT TAGAAGTCGG ACTACAGAGT TAGACTCAGA ACCAAAGGAG GTGGATAGGG
  421 GGTCCACAGG CCTGGTGCAG ATCACAGAGC CAGCCAGATC TGAGAAGCAG GGAACAAGAT
  481 GCTGGATTAA GCTTGGCATT CCGGTACTGT TGGTAAAGCC ACCATGGAAG ACGCCAAAAA
  541 CATAAAGAAA GGCCCGGCGC CATTCTATCC GCTGGAAGAT GGAACCGCTG GAGAGCAACT
  601 GCATAAGGCT ATGAAGAGAT ACGCCCTGGT TCCTGGAACA ATTGCTTTTA CAGATGCACA
  661 TATCGAGGTG GACATCACTT ACGCTGAGTA CTTCGAAATG TCCGTTCGGT TGGCAGAAGC
  721 TATGAAACGA TATGGGCTGA ATACAAATCA CAGAATCGTC GTATGCAGTG AAAACTCTCT
  781 TCAATTCTTT ATGCCGGTGT TGGGCGCGTT ATTTATCGGA GTTGCAGTTG CGCCCGCGAA
  841 CGACATTTAT AATGAACGTG AATTGCTCAA CAGTATGGGC ATTTCGCAGC CTACCGTGGT
  901 GTTCGTTTCC AAAAAGGGGT TGCAAAAAAA TTTTGAACGT GCAAAAAAAG CTCCCAATCA
  961 TCCAAAAAAT TATTATCATG GATTCTAAAA CGGATTACCA GGGATTTCAG TCGATGTACA
 1021 CGTTCGTCAC ATCTCATCTA CCTCCCGGGT TTTAATGAAT ACGATTTTTG TGCCAGAGTC
 1081 CCTTCGATTA GGACCAGAAC GATTTGCAAC TGAATCATGA AACCTCCCTC TCTGGGAAAT
1141 TCCTTAA
//
Primer: pGCAP_R
Sequence file: RDB15467_A7Job.seq check
>D04995A1_A7Jo_3_pGCAP_R_D01_04_ABI08.ab1
    1 AGTAACGTTA TCATGTCTGC TCGAAGCGGC CGGCCGCCCC GACTCTAGGG AATTCCGGAA
   61 TTCCGGAATT CCCTAGAATT ACACGGCGAT CTTTCCGCCC TTCTTGGCCT TTATGAGGAT
  121 CTCTCTGATT TTTCTTGCGT CGAGTTTTCC GGTAAGACCT TTCGGTACTT CGTCCACAAA
  181 CACAACTCCT CCGCGCAACT TTTTCGCGGT TGTTACTTGA CTGGCGACGT AATCCACGAT
  241 CTCTTTTTCC GTCATCGTCT TTCCGTGCTC CAAAACAACA ACGGCGGCGG GAAGTTCACC
  301 GGCGTCATCG TCGGGAAGAC CTGCGACACC TGCGTCGAAG ATGTTGGGGT GTTGGAGCAA
  361 GATGGATTCC AATTCAGCGG GAGCCACCTG ATAGCCTTTG TACTTAATCA GAGACTTCAG
  421 GCGGTCAACG ATGAAGAAGT GTTCGTCTTC GTCCCAGTAA GCTATGTCTC CAGAATGTAG
  481 CCATCCATCC TTGTCAATCA AGGCGTTGGT CGCTTCCGGA TTGTTTACAT AACCGGACAT
  541 AATCATAGGA CCTCTCACAC ACAGTTCGCC TCTTTGATTA ACGCCCAGCG TTTTCCCGGT
  601 ATCCAGATCC ACAACCTTCG CTTCAAAAAA TGGAACAACT TTACCGACCG CGCCCGGTTT
  661 ATCATCCCCC TCGGGTGTAA TCAGAATAGC TGATGTAGTC TCAGTGAGCC CATATCCTTG
  721 CCTGATACCT GGCAGATGGA ACCTCTTGGC AACCGCTTCC CCGACTTCCT TAGAGAGGGG
  781 AGCGCCACCA GAAGCAATTT CGTGTAAATT AGATAAATCG TATTTGTCAA TCAGAGTGCT
  841 TTTGGCGAAG AAGGAGAATA GGGTTGGCAC CAGCAGCGCA CTTTGAATCT TGTAATCCTG
  901 AAAGGCTCCT CAGAAACAGC TCTTCTTCAA ATCTATACAT TAAGACGACT CGAAATCCAC
  961 ATATCAAATA TCCGAGTGTA GTAAACATTC CAAAAACCGT GATGGAAATG GAACAACACT
 1021 TTAAAATCGC AGTAATCCCG GAAATGATTT GAATTGCCCA AAAAAATAGG AATCTCTGCA
 1081 TGCGAGAATC CTCACGCAGC CAGTTCTATG AGGCCAGAGC GAAACACCTT TAAGGCCAGA
1141 AACACCAGGT TAGAATT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kitada, S., BATF2 inhibits immunopathological Th17 responses by suppressing Il23a expression during Trypanosoma cruzi infection. J. Exp. Med. 214 (5): 1313-1331 (2017). PMID 28356392. [link to RRC of NBRP]