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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pFLAG-PDGFRA-C (#RDB15462)

Expression vector of human PDGFRA (deletion mutant with only the C-terminal portion), FLAG-tag.

Clone info. Expression vector of human PDGFRA (deletion mutant with only the C-terminal portion), tagged with FLAG at N-terminal.
Comment Expression was confirmed by the depositor with immunoprecipitation and immunoblotting.
Vector backbone pFLAG-CMV (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human PDGFRA cDNA
Depositor|Developer Kondo, Takeshi |
 
Sequence (full) RDB15462zzk02.seq provided by the depositor

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Ibata, M et al. Cancer Sci. 108 (2), 200-207, 2017).
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する（Ibata, M et al. Cancer Sci. 108 (2), 200-207, 2017）。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15462 pFLAG-PDGFRA-C DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pFLAG-PDGFRA-C was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15462).

Reference section:

Ibata, M., Iwasaki, J., Fujioka, Y., Nakagawa, K., Darmanin, S., Onozawa, M., Hashimoto, D., Ohba, Y., Hatakeyama, S., Teshima, T., Kondo, T., Leukemogenic kinase FIP1L1-PDGFRA and a small ubiquitin-like modifier E3 ligase, PIAS1, form a positive cross-talk through their enzymatic activities. Cancer Sci. 108 (2): 200-207 (2017). PMID 27960034. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15462_B2Cgp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro, FLAG, insert 5'
Sequence file: RDB15462_B2Cga.seq check
>D04855A9_B2Cg_2_ CMV_Forward_A06_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNCNNNN NNNNNNNNNG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
   61 ATAAGCAGAG CTCGTTTAGT GAACCGTCAG AATTAATTCA GATCGATCTA CCATGGACTA
  121 CAAAGACGAT GACGACAAGC TTGCGGCCGC GAATTCATCG ATAGATCTGA TATCGGTACC
  181 AGTCGACTCT AGAGGATCTA CTAGTCATAT GGATTGGTCT AGACTGCCAT ATGACTCAAG
  241 ATGGGAGTTT CCAAGAGATG GACTAGTGCT TGGTCGGGTC TTGGGGTCTG GAGCGTTTGG
  301 GAAGGTGGTT GAAGGAACAG CCTATGGATT AAGCCGGTCC CAACCTGTCA TGAAAGTTGC
  361 AGTGAAGATG CTAAAACCCA CGGCCAGATC CAGTGAAAAA CAAGCTCTCA TGTCTGAACT
  421 GAGGATAATG ACTCACCTGG GGCCACATTT GAACATCGTA AACTTGCTGG GAGCCTGCAC
  481 CAAGTCAGGC CCCATTTACA TCATCACAGA GTATTACTTC TATGGAGATT TGGTCAACTA
  541 TTTGCATAAG AATAGGGATA GCTTCCTGAG CCACCACCCA GAGAAGCCAA AGAAAGAGCT
  601 GGATATCTTT GGACTGAACC CTGCTGATGA AAGCACACGG AGCTATGTTA TTTTATCTTT
  661 TGAAAACAAT GATGACTACA TGGACATGAA GCANGCTGAT ACTACACAGT ATGTCCCCAT
  721 GCTAGAAAGG AAAGAGGNTT CTAAATATTC CGACNTCCAG AGATCACTCT ATGATCGTCC
  781 NGCCTCATAT AAGAAGAAAT CTATGTTAGA NTCNNAAGTC AAAAACCTCC TTTCNNGNGA
  841 NNNCTCANAN NNCTTACTTT ATTGGNNTTG NNGANCTTCN CCTATCNNNT NGCCCNANNN
  901 ANNNNNTTTT NNNCTNCAAA AANNGNGTCC NCNNNNATCN GGCNNNNCNC NNNNNNNNNN
//
Primer: hGHpolyA-R (Pr0350)
Region: hGH pA, insert 3'
Sequence file: RDB15462_B2Cgb.seq check
>D04855A9_B2Cg_2_ hGHpolyA-R_B06_06_ABI24.ab1
    1 NNNNNNNNCN NNNNNNNNNN NNAANANTAT AGAAGGANAC CTAGTCAGAC NAAATGATGC
   61 AACTTAATTT TATTAGGACA AGGCTGGTGG GCACTGGAGT GGCAACTTCC AGGGCCAGGA
  121 GAGGCACTGG GGAGGGGTCA CAGGGATGCC ACCCGGGATC CGATTATCTC GAGTTACAGG
  181 AAGCTGTCTT CCACCAGGTC TGAAGAGTCT ATGCCGATGT CGTCCATCAT GTCGATGTCT
  241 TCAATGGTCT CGTCCTCTCT CTTGATGAAG GTGGAACTGC TGGAACCCGT CTCAATGGCA
  301 CTCTCTTCAG AGGTCTGCGA GCTGTGTCTG TTCCTCTTGC CCAGGTCCTC CTCCTCAGGG
  361 ACAGGGTCAA TGTCAGGCAG AGGAATGATG TAGCCACTGT CAGCGCTCAG TCTCTGCTCA
  421 TCCAGACCAC CCTCCCAGTC CTTCAGCTTG TCTTCCTCGT TTTTGTAGGT GACACCAATG
  481 TATGCATTGT CTGAGTCCAC ACGCATGCGT GCCACAGCAG GATGGTCACT CTTCAGGAAG
  541 TCCAGGTGAA TTTTTTCATA ACTCTTTTTA TATTGTCCAG GCAGCAGATT CTCCACAATC
  601 TCACTCAGGT GGTAAAAGGA GGGTCTCTTC TCCGGCTCAC TGTTCCAGCA TTTCACCATG
  661 ATCTCGTANA CTTCACTGGT AGCGTGGTCA GGCTTGGCCA TCCGGTACCC ACTCTTGATC
  721 TTATTGTAGA AAGTAGAATC CACCATCATG CCNGGGTAAN GGGTGCCACC AAGGGAAAAN
  781 ATCTCCCANA NCANAANGNC ATAANANCAG ACATCACTCA NTGTGGNGTA GANGNTGNCA
  841 AAGANGNTCT CNNNANCCNT CCNCTTCNCG GGNAGAAAGG NACTGNCNCC CNNNNCATAN
  901 TTCNNNTCNT GNATGANGNN NNNNGNNNNN NNNNAANNCN CNNNNNNTNN NNNNTTTTNN
961 NNNNGNNNNN NNNNN
//
Primer: PDGFRA_F1 (Pr1076)
Region: insert mid
Sequence file: RDB15462_B2Cgc.seq check
>D04855A9_B2Cg_2_ PDGFRA_F1_D06_12_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NCNNGAAAGG NNNAGGNTTC NNAAATATTC CGACATCCAG
   61 AGATCACTCT ATGATCGTCC AGCCTCATAT AAGAAGAAAT CTATGTTAGA CTCAGAAGTC
  121 AAAAACCTCC TTTCAGGTGA TAACTCAGAA GGCCTTACTT TATTGGATTT GTTGAGCTTC
  181 ACCTATCAAG TTGCCCGAGG AATGGAGTTT TTGGCTTCAA AAAATTGTGT CCACCGTGAT
  241 CTGGCTGCTC GCAACGTCCT CCTGGCACAA GGAAAAATTG TGAAGATCTG TGACTTTGGC
  301 ATGGCCAGAG ACATCATGCA TGATTCGAAC TATGTGTCGG GAGGCAGTAC CTTTCTGCCC
  361 GTGAAGTGGA TGGCTCCTGA GAGCATCTTT GACAACCTCT ACACCACACT GAGTGATGTC
  421 TGGTCTTATG GCATTCTGCT CTGGGAGATC TTTTCCCTTG GTGGCACCCC TTACCCCGGC
  481 ATGATGGTGG ATTCTACTTT CTACAATAAG ATCAAGAGTG GGTACCGGAT GGCCAAGCCT
  541 GACCACGCTA CCAGTGAAGT CTACGAGATC ATGGTGAAAT GCTGGAACAG TGAGCCGGAG
  601 AAGAGACCCT CCTTTTACCA CCTGAGTGAG ATTGTGGAGA ATCTGCTGCC TGGACAATAT
  661 AAAAAGAGTT ATGAAAAAAT TCACCTGGAC TTCCTGAAGA GTGACCATCC TGCTGTGGCA
  721 CGCATGCGTG TGGACTCAGA CAATGCATAC ATTGGTGTCA CCTACAAAAA CGAGGAAGAN
  781 NAGCTGAANG ANTGNNANGG TGGTCTGGAT GANCAGANAN TGANCGCTGA NAGTGGCTAC
  841 NTCATTCCTC TGNCTGANNT NGNNCCNGTC CCTGANGANG ANNANCTGGN NAAGAGGAAN
  901 AGANNCNGCT CGNANANCTC NNANANAGTG NCNTTGANAN NGGTTNCNNC ANTTCNNNNN
961 TCNTCNNNAN ANANNANNAN ANNNTNNNNN NNTCGANNNN NNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori, NeoR
Sequence file: RDB15462_B2Cgd.seq check
>D04855A9_B2Cg_2_ SV40pro_ori_F_C06_09_ABI24.ab1
    1 NNNNNNNNNN NNNNGNNGCC GCCTCGNCCT CTGAGCTATT CCNGAAGTAG TGAGGAGGCT
   61 TTTTTGGAGG AATTGATCAG CTTGGGATCT GATCAAGAGA CAGGATGAGG ATCGTTTCGC
  121 ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC
  181 GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA
  241 GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG
  301 CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG
  361 CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG
  421 GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG
  481 CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC
  541 ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA
  601 GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG CATGCCCGAC
  661 GGCGAGGANC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT
  721 GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGNTGGGTG TGGCGGANCG CTATCANGAN
  781 ATANCGTTGG NTACCCGNGA NATTGCTGNA NANCTTGGCG GCGAATGGGC TGANCGCTTC
  841 CNCNNGCTTT ACGGNATCGC CGCTCCNNAT TCNNANCGCA TCNNNNNCNA TCNNNNNCNN
  901 NANNANNNNN NCTGANCGGN NNTCTGGGNN NCNNAATGAN NNANNNANNN NNNCCNNNNN
961 NNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ibata, M., Leukemogenic kinase FIP1L1-PDGFRA and a small ubiquitin-like modifier E3 ligase, PIAS1, form a positive cross-talk through their enzymatic activities. Cancer Sci. 108 (2): 200-207 (2017). PMID 27960034. [link to RRC of NBRP]
reference Iwasaki, J., FIP1L1 presence in FIP1L1-RARA or FIP1L1-PDGFRA differentially contributes to the pathogenesis of distinct types of leukemia. Ann. Hematol. 93 (9): 1473-1481 (2014). PMID 24763514.