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pFLAG-PDGFRA-C

Expression vector of human PDGFRA (deletion mutant with only the C-terminal portion), FLAG-tag.

Catalog number RDB15462
Resource name pFLAG-PDGFRA-C
Clone info. Expression vector of human PDGFRA (deletion mutant with only the C-terminal portion), tagged with FLAG at N-terminal.
Comment Expression was confirmed by the depositor with immunoprecipitation and immunoblotting.
Vector backbone pFLAG-CMV (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human PDGFRA cDNA
Depositor|Developer Kondo, Takeshi |
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External Database
human PDGFRA

          Reference sequence
            
           
          Sequence (full) RDB15462zzk02.seq provided by the depositor

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Ibata, M et al. Cancer Sci. 108 (2), 200-207, 2017).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ibata, M et al. Cancer Sci. 108 (2), 200-207, 2017)。
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          RDB15462 pFLAG-PDGFRA-C DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pFLAG-PDGFRA-C was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15462).

          Reference section:

          Ibata, M., Iwasaki, J., Fujioka, Y., Nakagawa, K., Darmanin, S., Onozawa, M., Hashimoto, D., Ohba, Y., Hatakeyama, S., Teshima, T., Kondo, T., Leukemogenic kinase FIP1L1-PDGFRA and a small ubiquitin-like modifier E3 ligase, PIAS1, form a positive cross-talk through their enzymatic activities. Cancer Sci. 108 (2): 200-207 (2017). PMID 27960034.

          Further references such as user reports and related articles (go to bottom)


          References and tips

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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15462_B2Cgp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: CMV pro, FLAG, insert 5'
          Sequence file: RDB15462_B2Cga.seq check
          >D04855A9_B2Cg_2_ CMV_Forward_A06_03_ABI24.ab1
              1 NNNNNNNNNN NNNNNCNNNN NNNNNNNNNG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
             61 ATAAGCAGAG CTCGTTTAGT GAACCGTCAG AATTAATTCA GATCGATCTA CCATGGACTA
            121 CAAAGACGAT GACGACAAGC TTGCGGCCGC GAATTCATCG ATAGATCTGA TATCGGTACC
            181 AGTCGACTCT AGAGGATCTA CTAGTCATAT GGATTGGTCT AGACTGCCAT ATGACTCAAG
            241 ATGGGAGTTT CCAAGAGATG GACTAGTGCT TGGTCGGGTC TTGGGGTCTG GAGCGTTTGG
            301 GAAGGTGGTT GAAGGAACAG CCTATGGATT AAGCCGGTCC CAACCTGTCA TGAAAGTTGC
            361 AGTGAAGATG CTAAAACCCA CGGCCAGATC CAGTGAAAAA CAAGCTCTCA TGTCTGAACT
            421 GAGGATAATG ACTCACCTGG GGCCACATTT GAACATCGTA AACTTGCTGG GAGCCTGCAC
            481 CAAGTCAGGC CCCATTTACA TCATCACAGA GTATTACTTC TATGGAGATT TGGTCAACTA
            541 TTTGCATAAG AATAGGGATA GCTTCCTGAG CCACCACCCA GAGAAGCCAA AGAAAGAGCT
            601 GGATATCTTT GGACTGAACC CTGCTGATGA AAGCACACGG AGCTATGTTA TTTTATCTTT
            661 TGAAAACAAT GATGACTACA TGGACATGAA GCANGCTGAT ACTACACAGT ATGTCCCCAT
            721 GCTAGAAAGG AAAGAGGNTT CTAAATATTC CGACNTCCAG AGATCACTCT ATGATCGTCC
            781 NGCCTCATAT AAGAAGAAAT CTATGTTAGA NTCNNAAGTC AAAAACCTCC TTTCNNGNGA
            841 NNNCTCANAN NNCTTACTTT ATTGGNNTTG NNGANCTTCN CCTATCNNNT NGCCCNANNN
            901 ANNNNNTTTT NNNCTNCAAA AANNGNGTCC NCNNNNATCN GGCNNNNCNC NNNNNNNNNN
          //
          Primer: hGHpolyA-R (Pr0350)
          Region: hGH pA, insert 3'
          Sequence file: RDB15462_B2Cgb.seq check
          >D04855A9_B2Cg_2_ hGHpolyA-R_B06_06_ABI24.ab1
              1 NNNNNNNNCN NNNNNNNNNN NNAANANTAT AGAAGGANAC CTAGTCAGAC NAAATGATGC
             61 AACTTAATTT TATTAGGACA AGGCTGGTGG GCACTGGAGT GGCAACTTCC AGGGCCAGGA
            121 GAGGCACTGG GGAGGGGTCA CAGGGATGCC ACCCGGGATC CGATTATCTC GAGTTACAGG
            181 AAGCTGTCTT CCACCAGGTC TGAAGAGTCT ATGCCGATGT CGTCCATCAT GTCGATGTCT
            241 TCAATGGTCT CGTCCTCTCT CTTGATGAAG GTGGAACTGC TGGAACCCGT CTCAATGGCA
            301 CTCTCTTCAG AGGTCTGCGA GCTGTGTCTG TTCCTCTTGC CCAGGTCCTC CTCCTCAGGG
            361 ACAGGGTCAA TGTCAGGCAG AGGAATGATG TAGCCACTGT CAGCGCTCAG TCTCTGCTCA
            421 TCCAGACCAC CCTCCCAGTC CTTCAGCTTG TCTTCCTCGT TTTTGTAGGT GACACCAATG
            481 TATGCATTGT CTGAGTCCAC ACGCATGCGT GCCACAGCAG GATGGTCACT CTTCAGGAAG
            541 TCCAGGTGAA TTTTTTCATA ACTCTTTTTA TATTGTCCAG GCAGCAGATT CTCCACAATC
            601 TCACTCAGGT GGTAAAAGGA GGGTCTCTTC TCCGGCTCAC TGTTCCAGCA TTTCACCATG
            661 ATCTCGTANA CTTCACTGGT AGCGTGGTCA GGCTTGGCCA TCCGGTACCC ACTCTTGATC
            721 TTATTGTAGA AAGTAGAATC CACCATCATG CCNGGGTAAN GGGTGCCACC AAGGGAAAAN
            781 ATCTCCCANA NCANAANGNC ATAANANCAG ACATCACTCA NTGTGGNGTA GANGNTGNCA
            841 AAGANGNTCT CNNNANCCNT CCNCTTCNCG GGNAGAAAGG NACTGNCNCC CNNNNCATAN
            901 TTCNNNTCNT GNATGANGNN NNNNGNNNNN NNNNAANNCN CNNNNNNTNN NNNNTTTTNN
          961 NNNNGNNNNN NNNNN
          //
          Primer: PDGFRA_F1 (Pr1076)
          Region: insert mid
          Sequence file: RDB15462_B2Cgc.seq check
          >D04855A9_B2Cg_2_ PDGFRA_F1_D06_12_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NCNNGAAAGG NNNAGGNTTC NNAAATATTC CGACATCCAG
             61 AGATCACTCT ATGATCGTCC AGCCTCATAT AAGAAGAAAT CTATGTTAGA CTCAGAAGTC
            121 AAAAACCTCC TTTCAGGTGA TAACTCAGAA GGCCTTACTT TATTGGATTT GTTGAGCTTC
            181 ACCTATCAAG TTGCCCGAGG AATGGAGTTT TTGGCTTCAA AAAATTGTGT CCACCGTGAT
            241 CTGGCTGCTC GCAACGTCCT CCTGGCACAA GGAAAAATTG TGAAGATCTG TGACTTTGGC
            301 ATGGCCAGAG ACATCATGCA TGATTCGAAC TATGTGTCGG GAGGCAGTAC CTTTCTGCCC
            361 GTGAAGTGGA TGGCTCCTGA GAGCATCTTT GACAACCTCT ACACCACACT GAGTGATGTC
            421 TGGTCTTATG GCATTCTGCT CTGGGAGATC TTTTCCCTTG GTGGCACCCC TTACCCCGGC
            481 ATGATGGTGG ATTCTACTTT CTACAATAAG ATCAAGAGTG GGTACCGGAT GGCCAAGCCT
            541 GACCACGCTA CCAGTGAAGT CTACGAGATC ATGGTGAAAT GCTGGAACAG TGAGCCGGAG
            601 AAGAGACCCT CCTTTTACCA CCTGAGTGAG ATTGTGGAGA ATCTGCTGCC TGGACAATAT
            661 AAAAAGAGTT ATGAAAAAAT TCACCTGGAC TTCCTGAAGA GTGACCATCC TGCTGTGGCA
            721 CGCATGCGTG TGGACTCAGA CAATGCATAC ATTGGTGTCA CCTACAAAAA CGAGGAAGAN
            781 NAGCTGAANG ANTGNNANGG TGGTCTGGAT GANCAGANAN TGANCGCTGA NAGTGGCTAC
            841 NTCATTCCTC TGNCTGANNT NGNNCCNGTC CCTGANGANG ANNANCTGGN NAAGAGGAAN
            901 AGANNCNGCT CGNANANCTC NNANANAGTG NCNTTGANAN NGGTTNCNNC ANTTCNNNNN
          961 TCNTCNNNAN ANANNANNAN ANNNTNNNNN NNTCGANNNN NNNNNN
          //
          Primer: SV40pro_ori_F (Pr0731)
          Region: SV40 pro_ori, NeoR
          Sequence file: RDB15462_B2Cgd.seq check
          >D04855A9_B2Cg_2_ SV40pro_ori_F_C06_09_ABI24.ab1
              1 NNNNNNNNNN NNNNGNNGCC GCCTCGNCCT CTGAGCTATT CCNGAAGTAG TGAGGAGGCT
             61 TTTTTGGAGG AATTGATCAG CTTGGGATCT GATCAAGAGA CAGGATGAGG ATCGTTTCGC
            121 ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC
            181 GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA
            241 GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG
            301 CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG
            361 CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG
            421 GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG
            481 CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC
            541 ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA
            601 GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG CATGCCCGAC
            661 GGCGAGGANC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT
            721 GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGNTGGGTG TGGCGGANCG CTATCANGAN
            781 ATANCGTTGG NTACCCGNGA NATTGCTGNA NANCTTGGCG GCGAATGGGC TGANCGCTTC
            841 CNCNNGCTTT ACGGNATCGC CGCTCCNNAT TCNNANCGCA TCNNNNNCNA TCNNNNNCNN
            901 NANNANNNNN NCTGANCGGN NNTCTGGGNN NCNNAATGAN NNANNNANNN NNNCCNNNNN
          961 NNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Ibata, M., Leukemogenic kinase FIP1L1-PDGFRA and a small ubiquitin-like modifier E3 ligase, PIAS1, form a positive cross-talk through their enzymatic activities. Cancer Sci. 108 (2): 200-207 (2017). PMID 27960034.
          reference Iwasaki, J., FIP1L1 presence in FIP1L1-RARA or FIP1L1-PDGFRA differentially contributes to the pathogenesis of distinct types of leukemia. Ann. Hematol. 93 (9): 1473-1481 (2014). PMID 24763514.

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl