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pREP1-BP+3'SS (#RDB15431)

Expression vector of S. pombe dg non-coding RNA gene SPNCRNA.232 genomic region containing dg intron with 3' branch point and splice site mutation.

Clone info. Expression vector of S. pombe dg non-coding RNA gene SPNCRNA.232 consisting of 570 bp upstream sequence, dg intron with 3' branch point and splice site mutation, and 106 bp downstream sequence with the nmt1 promoter and LEU2 marker. See Fig. 7 of Mutazono, M., PLoS Genet., 13 (2): e1006606, 2017.
Comment Expression was confirmed by the depositor with RT-PCR
Vector backbone pREP1 (plasmid)
Selectable markers Amp^r (E. coli), LEU2 (S. pombe).
Gene/insert name Schizosaccharomyces pombe 972h- miscRNA (SPNCRNA.232) genomic
Depositor|Developer Tani, Tokio |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Mutazono, M. et al. PLoS Genet. 13(2): e1006606)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mutazono, M. et al. PLoS Genet. 13(2): e1006606)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB15431 pREP1-BP+3'SS Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pREP1-BP+3'SS was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15431).

Reference section:

Mutazono, M., Morita, M., Tsukahara, C., Chinen, M., Nishioka, S., Yumikake, T., Dohke, K., Sakamoto, M., Ideue, T., Nakayama, J.I., Ishii, K., Tani, T., The intron in centromeric noncoding RNA facilitates RNAi-mediated formation of heterochromatin. PLoS Genet. 13 (2): e1006606 (2017). PMID 28231281. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Mutazono, M., The intron in centromeric noncoding RNA facilitates RNAi-mediated formation of heterochromatin. PLoS Genet. 13 (2): e1006606 (2017). PMID 28231281. [link to RRC of NBRP]