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pREP1-Short

Expression vector of S. pombe dg non-coding RNA gene SPNCRNA.232 genomic region containing dg intron.

Catalog number RDB15425
Resource name pREP1-Short
Clone info. Expression vector of S. pombe dg non-coding RNA gene SPNCRNA.232 consisting of shortened upstream sequence (146 bp), dg intron, and 106 bp downstream sequence with the nmt1 promoter and LEU2 marker. See Fig. 8 of Mutazono, M., PLoS Genet., 13 (2): e1006606, 2017.
Comment Expression was confirmed by the depositor with RT-PCR
Vector backbone pREP1 (plasmid)
Selectable markers Amp^r (E. coli), LEU2 (S. pombe).
Gene/insert name Schizosaccharomyces pombe 972h- miscRNA (SPNCRNA.232) genomic
Depositor|Developer Tani, Tokio |
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External Database
Schizosaccharomyces pombe 972h- miscRNA (SPNCRNA.232)
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Mutazono, M. et al. PLoS Genet. 13(2): e1006606)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mutazono, M. et al. PLoS Genet. 13(2): e1006606)。
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          RDB15425 pREP1-Short Under QC test. Please contact us. DNA solution

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          References and tips

          Electronic file

          Original reference

          original Mutazono, M., The intron in centromeric noncoding RNA facilitates RNAi-mediated formation of heterochromatin. PLoS Genet. 13 (2): e1006606 (2017). PMID 28231281.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Mutazono, M., The intron in centromeric noncoding RNA facilitates RNAi-mediated formation of heterochromatin. PLoS Genet. 13 (2): e1006606 (2017). PMID 28231281.

          2022.05.17

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